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The map label for this gene is clcA [H]

Identifier: 116494479

GI number: 116494479

Start: 955512

End: 957083

Strand: Direct

Name: clcA [H]

Synonym: LSEI_0973

Alternate gene names: 116494479

Gene position: 955512-957083 (Clockwise)

Preceding gene: 116494478

Following gene: 116494480

Centisome position: 33.0

GC content: 48.16

Gene sequence:

>1572_bases
TTGGCACGACCTGTAAAAGAAAAATTTGACGTTACTCGCCTGCGATTTGTGCTACTCGGACTACTCGTTGGTTTAATGAG
CGGCACTGTTGTCAGCGCGTTTCGCTATTGTATTGAAGTAGGGCTGCGCTATAGTCGGCTGGTATATGGGCACTTGCGAA
TAGCACCGTTTGTCTGGTGGCAATGGGCGTTGTTGATCGGTGTCAATCTTGGTTTGGCGTTGATCGTGGCGTGGCTTCTC
AAGAAAGAGCCCTATATTTCTGGCTCTGGGATTCCGCAAGTTGAAGGCCAATTGGCTGGTGAATTGGAAATGCACTGGTG
GTCGATCCTCTGGCGAAAATTTGTCGGCGGTGTTCTGGCGCTGGGGCCTGGGTTGTTTCTCGGACGGGAAGGACCGTCGA
TTCAGTTAGGCGCTTCAGTCGGGCAAGGTTTAGCTGCCGGGTTCAAGCTATCAGGAACTGATCGGCGATTGTTAATTGCA
TCCGGTTCTGCTGCAGGATTGGCGGCGGCATTTAATGCACCCATTGCTGGGACCTTGTTTGTCTTAGAAGAGGTTTATCA
TAATTTCTCACCGCTTGTTTGGTTAACGGCATTGGCTGGGGCAATTGGATCGGATTTTGTTTCACTGAATGTTTTCGGAC
TTGTTCCGGTGCTGCATTTAAGCTATTCGCGTAGTTTACCTGTATCGAGTTATTGGCATTTAATTTTGCTAGGCATTCTT
TTGGGCTTGTTTGGGTACTTATACCAGCGAATCTTGTTGGTGATGCCGCGGTGGTATCGCAACTTGACACATTTACCGCG
GCCATTACAAGGCATTGTACCGTTTCTGCTGGTGATTCTGGTTGGCTATTTTGCACCAAACTTACTTGGTGGTGGCAATG
GTTTGATTGTCGGTTTTGGCCAGTATGTGCCATCTTTGCTTGTATTGATCGCAATTTTTGTTGTACGTTTCGTTTTTTCA
ATGATTTCATATGGTTCTGGATTACCAGGTGGCATTTTTCTGCCGATTCTTTCTTTGGGTGCCGTGATTGGTGCCATTTA
TGGCGTCTTCATGAATCAACTCGGCTTATTGCCGCACGTCTATATCATGAACTTAATCATTTTTTCAATGGCCGGCTACT
TTGCTGGCATTGGGAAAGCACCGTTTACTGCGATTCTATTGGTAACGGAGATGGTTGGTAATTTGACGCATTTGATGCCG
TTAGCGGTCCTATCGTTAACTGCTTATCTCGTGGTTGATTTGCTTGGCGGTGCGCCGATCTACGAAGCGTTACTCGAGCA
AATGACAATGCCGAAAACAGTTCAACAGCTTCATCGACCCGACCGACTGGAAATACCGGTTTTCTTCGGCAGTCCCTTGA
ATGGCAAAATGGTCCGCGATATGCCATGGCCTAAAGAAGCATTACTAATCGGAATCCGGCGTGGCGAACAGGAAGTGATT
CCACATGGTGATTCCTTGATCCGTGAAGGTGATACGTTGGTGCTGTTAACGGATGCGACCCAACGTGCTCGGGTCAAGCG
GCGGATTGATGCGCTATCGGCGGCGTTGGGGAAAACGCACCAAAACTCATGA

Upstream 100 bases:

>100_bases
GAAAAACAATAATTGGTTTTGCTGACTCGGGTGACTGAGTCGTTTTTGATTCCCAGCCAGAGGTATATACTGAGATCATG
TTGTACTGGAGGGGAATTAC

Downstream 100 bases:

>100_bases
GCAAGTTATCTGTCAGAACGTTGACGTGCTATTATTGTGAGTTATTTTACTTGCTGTGAGACGGTTCAAGTAGCTAAAAA
GGTGGATTTCAGCAAAGATG

Product: chloride channel protein EriC

Products: Cl Ion [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MARPVKEKFDVTRLRFVLLGLLVGLMSGTVVSAFRYCIEVGLRYSRLVYGHLRIAPFVWWQWALLIGVNLGLALIVAWLL
KKEPYISGSGIPQVEGQLAGELEMHWWSILWRKFVGGVLALGPGLFLGREGPSIQLGASVGQGLAAGFKLSGTDRRLLIA
SGSAAGLAAAFNAPIAGTLFVLEEVYHNFSPLVWLTALAGAIGSDFVSLNVFGLVPVLHLSYSRSLPVSSYWHLILLGIL
LGLFGYLYQRILLVMPRWYRNLTHLPRPLQGIVPFLLVILVGYFAPNLLGGGNGLIVGFGQYVPSLLVLIAIFVVRFVFS
MISYGSGLPGGIFLPILSLGAVIGAIYGVFMNQLGLLPHVYIMNLIIFSMAGYFAGIGKAPFTAILLVTEMVGNLTHLMP
LAVLSLTAYLVVDLLGGAPIYEALLEQMTMPKTVQQLHRPDRLEIPVFFGSPLNGKMVRDMPWPKEALLIGIRRGEQEVI
PHGDSLIREGDTLVLLTDATQRARVKRRIDALSAALGKTHQNS

Sequences:

>Translated_523_residues
MARPVKEKFDVTRLRFVLLGLLVGLMSGTVVSAFRYCIEVGLRYSRLVYGHLRIAPFVWWQWALLIGVNLGLALIVAWLL
KKEPYISGSGIPQVEGQLAGELEMHWWSILWRKFVGGVLALGPGLFLGREGPSIQLGASVGQGLAAGFKLSGTDRRLLIA
SGSAAGLAAAFNAPIAGTLFVLEEVYHNFSPLVWLTALAGAIGSDFVSLNVFGLVPVLHLSYSRSLPVSSYWHLILLGIL
LGLFGYLYQRILLVMPRWYRNLTHLPRPLQGIVPFLLVILVGYFAPNLLGGGNGLIVGFGQYVPSLLVLIAIFVVRFVFS
MISYGSGLPGGIFLPILSLGAVIGAIYGVFMNQLGLLPHVYIMNLIIFSMAGYFAGIGKAPFTAILLVTEMVGNLTHLMP
LAVLSLTAYLVVDLLGGAPIYEALLEQMTMPKTVQQLHRPDRLEIPVFFGSPLNGKMVRDMPWPKEALLIGIRRGEQEVI
PHGDSLIREGDTLVLLTDATQRARVKRRIDALSAALGKTHQNS
>Mature_522_residues
ARPVKEKFDVTRLRFVLLGLLVGLMSGTVVSAFRYCIEVGLRYSRLVYGHLRIAPFVWWQWALLIGVNLGLALIVAWLLK
KEPYISGSGIPQVEGQLAGELEMHWWSILWRKFVGGVLALGPGLFLGREGPSIQLGASVGQGLAAGFKLSGTDRRLLIAS
GSAAGLAAAFNAPIAGTLFVLEEVYHNFSPLVWLTALAGAIGSDFVSLNVFGLVPVLHLSYSRSLPVSSYWHLILLGILL
GLFGYLYQRILLVMPRWYRNLTHLPRPLQGIVPFLLVILVGYFAPNLLGGGNGLIVGFGQYVPSLLVLIAIFVVRFVFSM
ISYGSGLPGGIFLPILSLGAVIGAIYGVFMNQLGLLPHVYIMNLIIFSMAGYFAGIGKAPFTAILLVTEMVGNLTHLMPL
AVLSLTAYLVVDLLGGAPIYEALLEQMTMPKTVQQLHRPDRLEIPVFFGSPLNGKMVRDMPWPKEALLIGIRRGEQEVIP
HGDSLIREGDTLVLLTDATQRARVKRRIDALSAALGKTHQNS

Specific function: Proton-coupled chloride transporter. Functions as antiport system and exchanges two chloride ions for 1 proton. Probably acts as an electrical shunt for an outwardly-directed proton pump that is linked to amino acid decarboxylation, as part of the extreme

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcA subfamily [H]

Homologues:

Organism=Homo sapiens, GI153252026, Length=519, Percent_Identity=25.4335260115607, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI4557473, Length=437, Percent_Identity=27.0022883295195, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI189217923, Length=437, Percent_Identity=26.7734553775744, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI189217921, Length=437, Percent_Identity=26.7734553775744, Blast_Score=97, Evalue=5e-20,
Organism=Homo sapiens, GI55770838, Length=438, Percent_Identity=25.1141552511416, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI55770840, Length=438, Percent_Identity=25.1141552511416, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1786350, Length=369, Percent_Identity=35.230352303523, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87081943, Length=323, Percent_Identity=30.6501547987616, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17532615, Length=431, Percent_Identity=24.8259860788863, Blast_Score=92, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6322500, Length=429, Percent_Identity=25.8741258741259, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24665008, Length=434, Percent_Identity=27.1889400921659, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45551566, Length=421, Percent_Identity=27.3159144893112, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 57015; Mature: 56884

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARPVKEKFDVTRLRFVLLGLLVGLMSGTVVSAFRYCIEVGLRYSRLVYGHLRIAPFVWW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QWALLIGVNLGLALIVAWLLKKEPYISGSGIPQVEGQLAGELEMHWWSILWRKFVGGVLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCHHHH
LGPGLFLGREGPSIQLGASVGQGLAAGFKLSGTDRRLLIASGSAAGLAAAFNAPIAGTLF
HCCCCEECCCCCCEEECCHHCCHHHCCCEECCCCCEEEEECCCCCHHHHHHCCCCHHHHH
VLEEVYHNFSPLVWLTALAGAIGSDFVSLNVFGLVPVLHLSYSRSLPVSSYWHLILLGIL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LGLFGYLYQRILLVMPRWYRNLTHLPRPLQGIVPFLLVILVGYFAPNLLGGGNGLIVGFG
HHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
QYVPSLLVLIAIFVVRFVFSMISYGSGLPGGIFLPILSLGAVIGAIYGVFMNQLGLLPHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YIMNLIIFSMAGYFAGIGKAPFTAILLVTEMVGNLTHLMPLAVLSLTAYLVVDLLGGAPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YEALLEQMTMPKTVQQLHRPDRLEIPVFFGSPLNGKMVRDMPWPKEALLIGIRRGEQEVI
HHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHHEEEHHCCCCCCC
PHGDSLIREGDTLVLLTDATQRARVKRRIDALSAALGKTHQNS
CCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ARPVKEKFDVTRLRFVLLGLLVGLMSGTVVSAFRYCIEVGLRYSRLVYGHLRIAPFVWW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
QWALLIGVNLGLALIVAWLLKKEPYISGSGIPQVEGQLAGELEMHWWSILWRKFVGGVLA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCHHHH
LGPGLFLGREGPSIQLGASVGQGLAAGFKLSGTDRRLLIASGSAAGLAAAFNAPIAGTLF
HCCCCEECCCCCCEEECCHHCCHHHCCCEECCCCCEEEEECCCCCHHHHHHCCCCHHHHH
VLEEVYHNFSPLVWLTALAGAIGSDFVSLNVFGLVPVLHLSYSRSLPVSSYWHLILLGIL
HHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LGLFGYLYQRILLVMPRWYRNLTHLPRPLQGIVPFLLVILVGYFAPNLLGGGNGLIVGFG
HHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHH
QYVPSLLVLIAIFVVRFVFSMISYGSGLPGGIFLPILSLGAVIGAIYGVFMNQLGLLPHV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
YIMNLIIFSMAGYFAGIGKAPFTAILLVTEMVGNLTHLMPLAVLSLTAYLVVDLLGGAPI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
YEALLEQMTMPKTVQQLHRPDRLEIPVFFGSPLNGKMVRDMPWPKEALLIGIRRGEQEVI
HHHHHHHCCCCHHHHHHCCCCCEEEEEEECCCCCCCEECCCCCCHHHHEEEHHCCCCCCC
PHGDSLIREGDTLVLLTDATQRARVKRRIDALSAALGKTHQNS
CCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cl Ion [Periplasm] [C]

Specific reaction: Cl Ion [Periplasm] = Cl Ion [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA