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The map label for this gene is pgk

Identifier: 116494474

GI number: 116494474

Start: 950097

End: 951287

Strand: Direct

Name: pgk

Synonym: LSEI_0968

Alternate gene names: 116494474

Gene position: 950097-951287 (Clockwise)

Preceding gene: 116494473

Following gene: 116494475

Centisome position: 32.82

GC content: 47.36

Gene sequence:

>1191_bases
TTGGCTAAATTAATTGTTTCAGACTTAGATGTTAAGGACAAAAAAGTCCTGATCCGCGTTGACTTCAACGTGCCGATCAA
AGATGGTGTTATCGGTGATGATAACCGGATCGTGGCAGCATTGCCAACCATCCAGTATGTTATTGATCATGGTGGAAAGG
CAATCCTCTTGTCACATCTCGGCCGTGTCAAGACCGAAGAAGACAAGGCTAAGCTGACCTTGAAGCCAGTTGCAGAACGT
TTGAGTGAATTGCTGAAGAAGCCAGTGACATTTGTCCCGGCAACTCGCGGCAAGGAACTTGAAGATGCTATCAACAAGAT
GAATGATGGCGACGTTCTTGTTATGGAAAACACCCGGTTTGAAGATCTTGACGGCAAGAAAGAATCCGGCAACGATCCTG
AACTTGGCAAGTACTGGGCAAGTCTTGGCGACTTATTCGTTAACGATGCCTTTGGGACTGCACACCGCAGCCATGCTTCA
AACGTCGGCATTGCTTCCAACATGAAGCAAACTGCTGCCGGCTTCTTGATGGAAAAGGAAATCAAGTTCTTAGGCGATGC
CGTTGACAACCCGAAACACCCATTCATTGCTATCTTGGGTGGTGCAAAGGTTTCCGATAAGATCGGCGTGATCGAAAACT
TGGTACCTAAGGCTGATAAGATTCTTATCGGCGGCGGCATGACCTATACTTTCTATGCTGCTAAGGGTATGAGCATCGGT
AAGTCTTTGGTTGAAAAAGACAAGATCGAACTTGCCAAGAAGATCATGGATCAGGCTGGCGACAAGCTGCTTTTGCCGGT
TGATTCCGTTGTTGCAACTGAATTCTCTAACGATGCCCCACATAAAGTTGTTGACGGCGACATTCCTGATGGCTATATGG
CCTTGGATATCGGCCCGAAGACAATCAAAGAATTCAAAGATGCTTTGCAAGGTGCCAAGACCGTTGTTTGGAATGGCCCA
ATGGGTGTCTTCGAAATGAGCAACTATGCTGAAGGAACCCTTGAAGTCGGCCGTGCCCTTGGCGACTTGAAGGATGCTAC
TACAATTATCGGCGGCGGCGATTCAACAGCTGCTGCTAAGCAATTGGGTATTGCACCTAAGATTACCCATATTTCCACTG
GCGGTGGCGCTAGCCTTGAATATCTTGAAGGCAAGACCTTGCCAGGTATCGCAGCTATTTCTGACAAGTAA

Upstream 100 bases:

>100_bases
ATGTTTTAGCATCCTGCTGAAAACGAGCGCTTATTTCTGGTGTTAAGCGGTCGTTACTTGAAGTTAAGTCATCTTTAGGA
AAAATAGGAGGTCATTTCTC

Downstream 100 bases:

>100_bases
TTGTCAGGCATGCTGATCTAACAGTCACTACCGGCAGACGGTGTCTGTCGGTACATATGAAAGGAAGATATGCCATGCGT
ACACCATTCATTGCTGGTAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGVIGDDNRIVAALPTIQYVIDHGGKAILLSHLGRVKTEEDKAKLTLKPVAER
LSELLKKPVTFVPATRGKELEDAINKMNDGDVLVMENTRFEDLDGKKESGNDPELGKYWASLGDLFVNDAFGTAHRSHAS
NVGIASNMKQTAAGFLMEKEIKFLGDAVDNPKHPFIAILGGAKVSDKIGVIENLVPKADKILIGGGMTYTFYAAKGMSIG
KSLVEKDKIELAKKIMDQAGDKLLLPVDSVVATEFSNDAPHKVVDGDIPDGYMALDIGPKTIKEFKDALQGAKTVVWNGP
MGVFEMSNYAEGTLEVGRALGDLKDATTIIGGGDSTAAAKQLGIAPKITHISTGGGASLEYLEGKTLPGIAAISDK

Sequences:

>Translated_396_residues
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGVIGDDNRIVAALPTIQYVIDHGGKAILLSHLGRVKTEEDKAKLTLKPVAER
LSELLKKPVTFVPATRGKELEDAINKMNDGDVLVMENTRFEDLDGKKESGNDPELGKYWASLGDLFVNDAFGTAHRSHAS
NVGIASNMKQTAAGFLMEKEIKFLGDAVDNPKHPFIAILGGAKVSDKIGVIENLVPKADKILIGGGMTYTFYAAKGMSIG
KSLVEKDKIELAKKIMDQAGDKLLLPVDSVVATEFSNDAPHKVVDGDIPDGYMALDIGPKTIKEFKDALQGAKTVVWNGP
MGVFEMSNYAEGTLEVGRALGDLKDATTIIGGGDSTAAAKQLGIAPKITHISTGGGASLEYLEGKTLPGIAAISDK
>Mature_395_residues
AKLIVSDLDVKDKKVLIRVDFNVPIKDGVIGDDNRIVAALPTIQYVIDHGGKAILLSHLGRVKTEEDKAKLTLKPVAERL
SELLKKPVTFVPATRGKELEDAINKMNDGDVLVMENTRFEDLDGKKESGNDPELGKYWASLGDLFVNDAFGTAHRSHASN
VGIASNMKQTAAGFLMEKEIKFLGDAVDNPKHPFIAILGGAKVSDKIGVIENLVPKADKILIGGGMTYTFYAAKGMSIGK
SLVEKDKIELAKKIMDQAGDKLLLPVDSVVATEFSNDAPHKVVDGDIPDGYMALDIGPKTIKEFKDALQGAKTVVWNGPM
GVFEMSNYAEGTLEVGRALGDLKDATTIIGGGDSTAAAKQLGIAPKITHISTGGGASLEYLEGKTLPGIAAISDK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=415, Percent_Identity=44.8192771084337, Blast_Score=324, Evalue=8e-89,
Organism=Homo sapiens, GI31543397, Length=417, Percent_Identity=42.9256594724221, Blast_Score=311, Evalue=6e-85,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=42.3173803526448, Blast_Score=297, Evalue=8e-82,
Organism=Caenorhabditis elegans, GI17508823, Length=416, Percent_Identity=46.875, Blast_Score=347, Evalue=8e-96,
Organism=Saccharomyces cerevisiae, GI6319857, Length=418, Percent_Identity=47.3684210526316, Blast_Score=342, Evalue=5e-95,
Organism=Drosophila melanogaster, GI17136394, Length=412, Percent_Identity=44.4174757281553, Blast_Score=314, Evalue=7e-86,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=43.6144578313253, Blast_Score=305, Evalue=3e-83,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_LACC3 (Q03AK6)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806208.1
- STRING:   Q03AK6
- GeneID:   4419209
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0968
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q03AK6
- ProtClustDB:   PRK00073
- BioCyc:   LCAS321967:LSEI_0968-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 42238; Mature: 42107

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 36-36 BINDING 119-119 BINDING 156-156 BINDING 207-207 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLIVSDLDVKDKKVLIRVDFNVPIKDGVIGDDNRIVAALPTIQYVIDHGGKAILLSHL
CCCEEEECCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECCHHHHHHHCCCCEEHHHHH
GRVKTEEDKAKLTLKPVAERLSELLKKPVTFVPATRGKELEDAINKMNDGDVLVMENTRF
CCCCCCCCHHEEEHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEEEECCCC
EDLDGKKESGNDPELGKYWASLGDLFVNDAFGTAHRSHASNVGIASNMKQTAAGFLMEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
IKFLGDAVDNPKHPFIAILGGAKVSDKIGVIENLVPKADKILIGGGMTYTFYAAKGMSIG
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCHHH
KSLVEKDKIELAKKIMDQAGDKLLLPVDSVVATEFSNDAPHKVVDGDIPDGYMALDIGPK
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEECCCCCCCEEEEECCHH
TIKEFKDALQGAKTVVWNGPMGVFEMSNYAEGTLEVGRALGDLKDATTIIGGGDSTAAAK
HHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHH
QLGIAPKITHISTGGGASLEYLEGKTLPGIAAISDK
HHCCCCEEEEEECCCCCEEEEECCCCCCCEECCCCC
>Mature Secondary Structure 
AKLIVSDLDVKDKKVLIRVDFNVPIKDGVIGDDNRIVAALPTIQYVIDHGGKAILLSHL
CCEEEECCCCCCCEEEEEEECCCCCCCCEECCCCEEEEECCHHHHHHHCCCCEEHHHHH
GRVKTEEDKAKLTLKPVAERLSELLKKPVTFVPATRGKELEDAINKMNDGDVLVMENTRF
CCCCCCCCHHEEEHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHCCCCCEEEEECCCC
EDLDGKKESGNDPELGKYWASLGDLFVNDAFGTAHRSHASNVGIASNMKQTAAGFLMEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHH
IKFLGDAVDNPKHPFIAILGGAKVSDKIGVIENLVPKADKILIGGGMTYTFYAAKGMSIG
HHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCHHH
KSLVEKDKIELAKKIMDQAGDKLLLPVDSVVATEFSNDAPHKVVDGDIPDGYMALDIGPK
HHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHCCCCCCCCEECCCCCCCEEEEECCHH
TIKEFKDALQGAKTVVWNGPMGVFEMSNYAEGTLEVGRALGDLKDATTIIGGGDSTAAAK
HHHHHHHHHCCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHCEEEEECCCCHHHHH
QLGIAPKITHISTGGGASLEYLEGKTLPGIAAISDK
HHCCCCEEEEEECCCCCEEEEECCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA