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The map label for this gene is polC [H]

Identifier: 116493923

GI number: 116493923

Start: 355948

End: 356853

Strand: Direct

Name: polC [H]

Synonym: LSEI_0356

Alternate gene names: 116493923

Gene position: 355948-356853 (Clockwise)

Preceding gene: 116493922

Following gene: 116493927

Centisome position: 12.29

GC content: 50.77

Gene sequence:

>906_bases
ATGATCGTGATCACGTTATCCAAAGTACCGCCATCGCTCAGAGGCGTTCTGACGAAGTGGTGCCAAGAGGTCCAGACCGG
GGTCTATGTTGGCCGGTTTAGCGCGAGAATCCGCGACTCTTTATGGGAACGGATCCAGCGTGATATCGGGTCGGGCGAGG
CCACCATCGTCTTTAACGCAAAAAACGAACTCGGCTACCAGTTCAGAACTACCCGGACGGATCGTGAGGTCGTCGATTAC
GATGGGTTACCGCTCTTGCTGAGAAAGAACACGAACCCTGAATTGGTCCAGCTTGGCTTCAGCAACGCCGCAAAGTTTCG
CAAAGCCAAGAAATTTGCCCGAGGAAAGCCGCCGACGAACGCGACGAAGGTTGCTGAAGGCGTTCCATTTGTCGCCTTGG
ATATTGAAACTACCGGATTGGATGCTGAACGAGATGCAATTATTGCCATTGGGGCGGTAAAAGGACCGTCAGTTAACGAA
ACCGAAAAATTCACTACCTTGGTGAATCCACGAAGGCCGCTTCCATCAACGGTTGCCAAGTTGACGGGATTGTCGGCGGA
GGAGTTGGTTGCCAATGGGATCAGTCTGAGCGAGGCAGTGCAGGCCTTACGGGATTTTACCGGCTCGCTCCCTTTAGTCG
GATATAATCTGCGTTTCGACATGGGATTTCTTTCGGCAGCGGGTCGTGAAACTGGGTTAACGTCTTTTGGGAATCAGACT
TTTGATTTACTACCGCTTGTTAAGAAATCCAGTCGCTTTCTGGATAATTACAAGCTTAGTACAGTGCTGGCACATTATCA
GATCGATAACGAGCAACCGCATCACGCGTTGTCTGATGCGACGGCAACAATGGCACTGGCGGACAAACTCATAAAAAAAG
GGATCTTAACGATTGGCAAACGTTGA

Upstream 100 bases:

>100_bases
ACGATGATTTGGACGCGGACGTGGTCAATCTCTGGGATGATAAGCTTGGACTGCAGAAATTCGGCGTGTCCTACCGTGAG
TCAACGGAGGACGGGCAATC

Downstream 100 bases:

>100_bases
TGGGATAGGGATTTTTTAGTGTTTTCCCCGCACATGCGGGGGTGATCCCTTCTCTGACTGGCTTACCGCTTCTTTGCATT
TGTTTTCCCCGCACATGCGG

Product: 3'-5' exonuclease and CRISPR-associated Cas2 family protein

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MIVITLSKVPPSLRGVLTKWCQEVQTGVYVGRFSARIRDSLWERIQRDIGSGEATIVFNAKNELGYQFRTTRTDREVVDY
DGLPLLLRKNTNPELVQLGFSNAAKFRKAKKFARGKPPTNATKVAEGVPFVALDIETTGLDAERDAIIAIGAVKGPSVNE
TEKFTTLVNPRRPLPSTVAKLTGLSAEELVANGISLSEAVQALRDFTGSLPLVGYNLRFDMGFLSAAGRETGLTSFGNQT
FDLLPLVKKSSRFLDNYKLSTVLAHYQIDNEQPHHALSDATATMALADKLIKKGILTIGKR

Sequences:

>Translated_301_residues
MIVITLSKVPPSLRGVLTKWCQEVQTGVYVGRFSARIRDSLWERIQRDIGSGEATIVFNAKNELGYQFRTTRTDREVVDY
DGLPLLLRKNTNPELVQLGFSNAAKFRKAKKFARGKPPTNATKVAEGVPFVALDIETTGLDAERDAIIAIGAVKGPSVNE
TEKFTTLVNPRRPLPSTVAKLTGLSAEELVANGISLSEAVQALRDFTGSLPLVGYNLRFDMGFLSAAGRETGLTSFGNQT
FDLLPLVKKSSRFLDNYKLSTVLAHYQIDNEQPHHALSDATATMALADKLIKKGILTIGKR
>Mature_301_residues
MIVITLSKVPPSLRGVLTKWCQEVQTGVYVGRFSARIRDSLWERIQRDIGSGEATIVFNAKNELGYQFRTTRTDREVVDY
DGLPLLLRKNTNPELVQLGFSNAAKFRKAKKFARGKPPTNATKVAEGVPFVALDIETTGLDAERDAIIAIGAVKGPSVNE
TEKFTTLVNPRRPLPSTVAKLTGLSAEELVANGISLSEAVQALRDFTGSLPLVGYNLRFDMGFLSAAGRETGLTSFGNQT
FDLLPLVKKSSRFLDNYKLSTVLAHYQIDNEQPHHALSDATATMALADKLIKKGILTIGKR

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI87082153, Length=87, Percent_Identity=41.3793103448276, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 32979; Mature: 32979

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVITLSKVPPSLRGVLTKWCQEVQTGVYVGRFSARIRDSLWERIQRDIGSGEATIVFNA
CEEEEECCCCHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
KNELGYQFRTTRTDREVVDYDGLPLLLRKNTNPELVQLGFSNAAKFRKAKKFARGKPPTN
CCCCCCEEEECCCCCCEECCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
ATKVAEGVPFVALDIETTGLDAERDAIIAIGAVKGPSVNETEKFTTLVNPRRPLPSTVAK
HHHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHH
LTGLSAEELVANGISLSEAVQALRDFTGSLPLVGYNLRFDMGFLSAAGRETGLTSFGNQT
HHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCCCCCCCCCCCC
FDLLPLVKKSSRFLDNYKLSTVLAHYQIDNEQPHHALSDATATMALADKLIKKGILTIGK
HHHHHHHHHHHHHHHCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
R
C
>Mature Secondary Structure
MIVITLSKVPPSLRGVLTKWCQEVQTGVYVGRFSARIRDSLWERIQRDIGSGEATIVFNA
CEEEEECCCCHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
KNELGYQFRTTRTDREVVDYDGLPLLLRKNTNPELVQLGFSNAAKFRKAKKFARGKPPTN
CCCCCCEEEECCCCCCEECCCCCEEEEECCCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
ATKVAEGVPFVALDIETTGLDAERDAIIAIGAVKGPSVNETEKFTTLVNPRRPLPSTVAK
HHHHHCCCCEEEEEEEECCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCHHHHHH
LTGLSAEELVANGISLSEAVQALRDFTGSLPLVGYNLRFDMGFLSAAGRETGLTSFGNQT
HHCCCHHHHHHCCCCHHHHHHHHHHHCCCCCEEEEEEEECCHHHHHCCCCCCCCCCCCCC
FDLLPLVKKSSRFLDNYKLSTVLAHYQIDNEQPHHALSDATATMALADKLIKKGILTIGK
HHHHHHHHHHHHHHHCHHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]