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The map label for this gene is yojA [H]

Identifier: 116493800

GI number: 116493800

Start: 225291

End: 226643

Strand: Direct

Name: yojA [H]

Synonym: LSEI_0229

Alternate gene names: 116493800

Gene position: 225291-226643 (Clockwise)

Preceding gene: 116493799

Following gene: 116493802

Centisome position: 7.78

GC content: 48.93

Gene sequence:

>1353_bases
ATGGATATACTCGTCCTGGTCTTAGGTATCCTGCTCTTACTGGTGCTTATCATCAAGTTTAAGTTCAACACGTATGTTTC
CTTGATCATCACAGCTGTCGTCGTCGCCCTTGGTTTGGGAATGGCGCCAGCCAAGATTGCGACCAGTATTCAAAATGGGA
TTGGCGCGCAACTAGGTGAGCTGGCCTTGGTCTTCGGCTTCGGTGCCATGCTTGGTCGATTGGTTGCTGATGCTGGTGGA
GCTTACCGCATTGCGCATACCCTCATCAATGCCTTTGGTCGCCGCGGATTGCAACTTGCCGTTGTTTTGGCATCTTTCAT
TATCGGTATTGCTTTGTTCTTTGAAGTTGGGATTGTCTTGCTGGTTCCAATTGTTTTCGCCATTGCGGCTGAAGCAGGCG
TTCCAATTCTGACACTTGGTATCCCGATGGCAGCGGCTTTGTCGGTGACCCATGGGTTCTTGCCGCCACATCCAGCTCCA
ACGGCGATCTCGACTGCATTAGGCGCTTCTGCTGGTCTGGTTTTGGCCTATGGCGTGATCATCGCCATTCCGACTGTTTA
TATTGCCGGACCGTTGTTTTCCAAACTTGCGCATCGCTTTGTGCCAGATGCTTTTGAAAGGCGCGGTAATTTGAAAGCAT
TGGGGCCGCAAAAAACCTTTAAATTGGAGGAAACACCAAGTTTTGGCATCTCCGTTCTCACATCACTTTTCCCAGTGATT
TTGATGGCGATTGCCACGGTTTATGAATTGGTATTTCATGGTGGCAAACTTCCAAAGACACCAAATGGTCTTGATAACGT
TATCGCATTCATTGGTTCACCAAGTATCGCCATGCTGATTTCATTGTTGTTCGCTATGTGGGCAATGGGCTATGCGCGCA
AGCTGCCAGCCAAAGCCATTATGACAACCCTCGAGGACGCTGTGAAATCAATTGCGATGTTACTACTCGTCATTGGTGGC
GGCGGTGCCTTCAAACAAGTCCTGATTGATGGTGGCGTCGGCGACTCAGTAAAAAATTTGTTCGTTGGCTCAGGCATTTC
ACCACTTATCTTGGGTTGGCTGATTGCGGTTGTCTTACGAATTGCACTGGGATCCGCGACTGTTGCGGCCATGACGGCCT
CTGGACTGGTTCTGCCACTCATGCAAAGTGCTGGCATCCAACCAGCTTTGATGGTACTGGCAATTGGTGCGGGTTCATTA
GCTGCCAGTCACGTTAATGACGCCGGTTTCTGGATGTTTCGTGAGTACTTTGACTTGACCATTAAACAGACCTTGTTGAC
GTGGACATTGTTAGAAACCATTATTTCAGTTGTTGGACTTGGTGGGGTGCTGTTGTTGAGTTTGGTGGTTTAA

Upstream 100 bases:

>100_bases
ACAGATCAAACGACATGTCTCGAGAAAAACGTCAATGAACGTTTTTCATAAATAAAATTGTAAGCGATTTCTTAATCTGC
AAAATTTAGGGGGAATAGAA

Downstream 100 bases:

>100_bases
GAGTAGCTGAGGAAACTTAGGACATTCAATCTAAGCAAAATGATCTTAGAGAGGAGCTTATCTTTAAGATCATTTTTTCT
ATTCAAAAAACAAACGGCAT

Product: H+/gluconate symporter related permease

Products: gluconate [Cytoplasm]; L-idonate [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDILVLVLGILLLLVLIIKFKFNTYVSLIITAVVVALGLGMAPAKIATSIQNGIGAQLGELALVFGFGAMLGRLVADAGG
AYRIAHTLINAFGRRGLQLAVVLASFIIGIALFFEVGIVLLVPIVFAIAAEAGVPILTLGIPMAAALSVTHGFLPPHPAP
TAISTALGASAGLVLAYGVIIAIPTVYIAGPLFSKLAHRFVPDAFERRGNLKALGPQKTFKLEETPSFGISVLTSLFPVI
LMAIATVYELVFHGGKLPKTPNGLDNVIAFIGSPSIAMLISLLFAMWAMGYARKLPAKAIMTTLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDSVKNLFVGSGISPLILGWLIAVVLRIALGSATVAAMTASGLVLPLMQSAGIQPALMVLAIGAGSL
AASHVNDAGFWMFREYFDLTIKQTLLTWTLLETIISVVGLGGVLLLSLVV

Sequences:

>Translated_450_residues
MDILVLVLGILLLLVLIIKFKFNTYVSLIITAVVVALGLGMAPAKIATSIQNGIGAQLGELALVFGFGAMLGRLVADAGG
AYRIAHTLINAFGRRGLQLAVVLASFIIGIALFFEVGIVLLVPIVFAIAAEAGVPILTLGIPMAAALSVTHGFLPPHPAP
TAISTALGASAGLVLAYGVIIAIPTVYIAGPLFSKLAHRFVPDAFERRGNLKALGPQKTFKLEETPSFGISVLTSLFPVI
LMAIATVYELVFHGGKLPKTPNGLDNVIAFIGSPSIAMLISLLFAMWAMGYARKLPAKAIMTTLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDSVKNLFVGSGISPLILGWLIAVVLRIALGSATVAAMTASGLVLPLMQSAGIQPALMVLAIGAGSL
AASHVNDAGFWMFREYFDLTIKQTLLTWTLLETIISVVGLGGVLLLSLVV
>Mature_450_residues
MDILVLVLGILLLLVLIIKFKFNTYVSLIITAVVVALGLGMAPAKIATSIQNGIGAQLGELALVFGFGAMLGRLVADAGG
AYRIAHTLINAFGRRGLQLAVVLASFIIGIALFFEVGIVLLVPIVFAIAAEAGVPILTLGIPMAAALSVTHGFLPPHPAP
TAISTALGASAGLVLAYGVIIAIPTVYIAGPLFSKLAHRFVPDAFERRGNLKALGPQKTFKLEETPSFGISVLTSLFPVI
LMAIATVYELVFHGGKLPKTPNGLDNVIAFIGSPSIAMLISLLFAMWAMGYARKLPAKAIMTTLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDSVKNLFVGSGISPLILGWLIAVVLRIALGSATVAAMTASGLVLPLMQSAGIQPALMVLAIGAGSL
AASHVNDAGFWMFREYFDLTIKQTLLTWTLLETIISVVGLGGVLLLSLVV

Specific function: Transport Of Gluconate And L-Idonate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=453, Percent_Identity=51.2141280353201, Blast_Score=422, Evalue=1e-119,
Organism=Escherichia coli, GI1790716, Length=449, Percent_Identity=49.6659242761693, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI2367370, Length=451, Percent_Identity=51.6629711751663, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1790776, Length=455, Percent_Identity=41.978021978022, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1788707, Length=453, Percent_Identity=35.5408388520971, Blast_Score=253, Evalue=2e-68,
Organism=Escherichia coli, GI48994939, Length=446, Percent_Identity=35.2017937219731, Blast_Score=228, Evalue=9e-61,
Organism=Escherichia coli, GI1789097, Length=437, Percent_Identity=32.4942791762014, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 46680; Mature: 46680

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILVLVLGILLLLVLIIKFKFNTYVSLIITAVVVALGLGMAPAKIATSIQNGIGAQLGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
LALVFGFGAMLGRLVADAGGAYRIAHTLINAFGRRGLQLAVVLASFIIGIALFFEVGIVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVPIVFAIAAEAGVPILTLGIPMAAALSVTHGFLPPHPAPTAISTALGASAGLVLAYGVI
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
IAIPTVYIAGPLFSKLAHRFVPDAFERRGNLKALGPQKTFKLEETPSFGISVLTSLFPVI
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
LMAIATVYELVFHGGKLPKTPNGLDNVIAFIGSPSIAMLISLLFAMWAMGYARKLPAKAI
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
MTTLEDAVKSIAMLLLVIGGGGAFKQVLIDGGVGDSVKNLFVGSGISPLILGWLIAVVLR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
IALGSATVAAMTASGLVLPLMQSAGIQPALMVLAIGAGSLAASHVNDAGFWMFREYFDLT
HHHCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHH
IKQTLLTWTLLETIISVVGLGGVLLLSLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MDILVLVLGILLLLVLIIKFKFNTYVSLIITAVVVALGLGMAPAKIATSIQNGIGAQLGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
LALVFGFGAMLGRLVADAGGAYRIAHTLINAFGRRGLQLAVVLASFIIGIALFFEVGIVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVPIVFAIAAEAGVPILTLGIPMAAALSVTHGFLPPHPAPTAISTALGASAGLVLAYGVI
HHHHHHHHHHHCCCCEEEECHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCHHHHHHHHH
IAIPTVYIAGPLFSKLAHRFVPDAFERRGNLKALGPQKTFKLEETPSFGISVLTSLFPVI
HHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHH
LMAIATVYELVFHGGKLPKTPNGLDNVIAFIGSPSIAMLISLLFAMWAMGYARKLPAKAI
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHH
MTTLEDAVKSIAMLLLVIGGGGAFKQVLIDGGVGDSVKNLFVGSGISPLILGWLIAVVLR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
IALGSATVAAMTASGLVLPLMQSAGIQPALMVLAIGAGSLAASHVNDAGFWMFREYFDLT
HHHCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHH
IKQTLLTWTLLETIISVVGLGGVLLLSLVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gluconate [Periplasm]; Proton [Periplasm]; L-idonate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] Proton [Periplasm] + L-idonate [Periplasm] = L-idonate [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]