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The map label for this gene is murE

Identifier: 116493794

GI number: 116493794

Start: 217922

End: 219475

Strand: Direct

Name: murE

Synonym: LSEI_0223

Alternate gene names: 116493794

Gene position: 217922-219475 (Clockwise)

Preceding gene: 116493793

Following gene: 116493795

Centisome position: 7.53

GC content: 46.85

Gene sequence:

>1554_bases
ATGGCACTATCACTTAGAGCATGTATCTTATTATTAAAGGAACATCATCTGCTAAAGAGTGCAGCAATTCAGAATACTGA
AGATGTCGATATGACCGGCATCGCCTATGATTCACGCAAGGTCTCTGGACCAACGCTGTTTTTCTGCAAAGGCGACAAGT
TCCGACCAATTTATCTTTCCATGGCCAAGGACAACGGTGCGCGAACTTATGTTGCCGAGAAACCTTACGTTGAAGGCAAC
GGCTTAAACGCCTTGATCGTGCGGAATATTACTAAAGCAATGGCGATTTTGAGCGCTGCTTTCTTTGATTATCCGCAAGA
TGATTTGTTTGTGATCGCTTATACGGGGACCAAGGGGAAAACCACGGCTTCCTATTTTACAGAAGCTATTTTAAATGAGG
CTCGTCCACGACACATCGCGCTTTTTTCTACAATTGATACGGTTGTTGGCCCTGAGCCCGATCAGCGTTTCAAGTCTAAT
CTGACAACGCCAGAGAGCCTCGATCTTTTCCGCGATATGCGCGAGGCTGTCGAAAACGGTATGACCCATCTCGTGATGGA
AGTGTCGAGTCAGGCATATTTGCGCAATCGCGTTTTTGGATTGACGTATGACGTCGGCTTCTTCCTGAACATTACGCCAG
ATCACATCGGACCGAATGAACATCCAACCTTTGCTAATTACTTACACAACAAGCTGCAATTGTTGGTCAATGCGCGCAAA
GTGGTCATTAATGCCGAAACCGAGCATTTTGACCAGGTTTATGCTGCCGCAACGACCACCACTTATCCAGAAAGCATCTA
CCTGTTTGCCAGTGCTGGCTTCAGACCTAAACGCGATGATATCGATATTGATTTTCGCTTTGATAGTCAGGAAGCTGACG
TTGCTGAGAGTCGTTTCACTTTAGTACCAGTCACGGAAAAAGCAGCAGCATTGAACATCGGCGGTCACTACTCGCTTGCT
TTGATTGGTGATTTTAACGAAAGTAACGCGACGGCTGCTATTATTGGTGCTGGATTGGCTGGCGTGGATGCATCTGCCGC
TGTTCCGGGTATTGCAAAAGTTAAAATCCCCGGCCGCATGGAGCATACGAAAGTTGCCGGACATGGTGCTGTCTATGTTG
ATTATGCGCACAATTACGCTAGTATGAAGGCGTTATTGTCGTTTTTGAAACACGCTTACAAGGATCCTCGATTACTGGTT
GTGGTTGGATCCCCCGGTGATAAAGGTGTTTCTCGTCGTCCCGGATTTGCTAAGGTTCTCACTGAGTTTGCAACTGAGGC
ATTCTTGACTACCGACGATCCTGGGTACGAAGATCCAGCAAACATCATTGAGGAAATCGATAGTAAAATCGATCATAGCA
AAGTTAAAGTACACAAAGTGCTTGATCGGAAAAAGGCCATTGCTGAAGCTATTAGCGACAGCGGACCAAATGATATCGTG
GTAGTGGCTGGCAAAGGGGCCGACCCCTATCAAAAGGTACGCGGCGTCAACACACCATGGCCAACAGACATGGCTGTTGT
AAAGCAAGTCGCTAAATCGCTACAGGAAGGCTGA

Upstream 100 bases:

>100_bases
ACACAGATAGTTGTGACAGTCCGACTGGTTTAATGCCAGTACCGCGCTTATGCTATAATGAAGCTTGAATTCACGGCATA
TAAAAAGGAGTAGCAGCCCA

Downstream 100 bases:

>100_bases
TACGAATGCGTCAATTTTTCACCGTTATTGGCGGCATGGGCACGCCGGCAACCGAGAGTTATGTCCGTTTGTTAAACCAG
CGTACCCACGCGCACAGCGA

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: ADP; H+; Phosphate; UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate [C]

Alternate protein names: UDP-MurNAc-tripeptide synthetase

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MALSLRACILLLKEHHLLKSAAIQNTEDVDMTGIAYDSRKVSGPTLFFCKGDKFRPIYLSMAKDNGARTYVAEKPYVEGN
GLNALIVRNITKAMAILSAAFFDYPQDDLFVIAYTGTKGKTTASYFTEAILNEARPRHIALFSTIDTVVGPEPDQRFKSN
LTTPESLDLFRDMREAVENGMTHLVMEVSSQAYLRNRVFGLTYDVGFFLNITPDHIGPNEHPTFANYLHNKLQLLVNARK
VVINAETEHFDQVYAAATTTTYPESIYLFASAGFRPKRDDIDIDFRFDSQEADVAESRFTLVPVTEKAAALNIGGHYSLA
LIGDFNESNATAAIIGAGLAGVDASAAVPGIAKVKIPGRMEHTKVAGHGAVYVDYAHNYASMKALLSFLKHAYKDPRLLV
VVGSPGDKGVSRRPGFAKVLTEFATEAFLTTDDPGYEDPANIIEEIDSKIDHSKVKVHKVLDRKKAIAEAISDSGPNDIV
VVAGKGADPYQKVRGVNTPWPTDMAVVKQVAKSLQEG

Sequences:

>Translated_517_residues
MALSLRACILLLKEHHLLKSAAIQNTEDVDMTGIAYDSRKVSGPTLFFCKGDKFRPIYLSMAKDNGARTYVAEKPYVEGN
GLNALIVRNITKAMAILSAAFFDYPQDDLFVIAYTGTKGKTTASYFTEAILNEARPRHIALFSTIDTVVGPEPDQRFKSN
LTTPESLDLFRDMREAVENGMTHLVMEVSSQAYLRNRVFGLTYDVGFFLNITPDHIGPNEHPTFANYLHNKLQLLVNARK
VVINAETEHFDQVYAAATTTTYPESIYLFASAGFRPKRDDIDIDFRFDSQEADVAESRFTLVPVTEKAAALNIGGHYSLA
LIGDFNESNATAAIIGAGLAGVDASAAVPGIAKVKIPGRMEHTKVAGHGAVYVDYAHNYASMKALLSFLKHAYKDPRLLV
VVGSPGDKGVSRRPGFAKVLTEFATEAFLTTDDPGYEDPANIIEEIDSKIDHSKVKVHKVLDRKKAIAEAISDSGPNDIV
VVAGKGADPYQKVRGVNTPWPTDMAVVKQVAKSLQEG
>Mature_516_residues
ALSLRACILLLKEHHLLKSAAIQNTEDVDMTGIAYDSRKVSGPTLFFCKGDKFRPIYLSMAKDNGARTYVAEKPYVEGNG
LNALIVRNITKAMAILSAAFFDYPQDDLFVIAYTGTKGKTTASYFTEAILNEARPRHIALFSTIDTVVGPEPDQRFKSNL
TTPESLDLFRDMREAVENGMTHLVMEVSSQAYLRNRVFGLTYDVGFFLNITPDHIGPNEHPTFANYLHNKLQLLVNARKV
VINAETEHFDQVYAAATTTTYPESIYLFASAGFRPKRDDIDIDFRFDSQEADVAESRFTLVPVTEKAAALNIGGHYSLAL
IGDFNESNATAAIIGAGLAGVDASAAVPGIAKVKIPGRMEHTKVAGHGAVYVDYAHNYASMKALLSFLKHAYKDPRLLVV
VGSPGDKGVSRRPGFAKVLTEFATEAFLTTDDPGYEDPANIIEEIDSKIDHSKVKVHKVLDRKKAIAEAISDSGPNDIVV
VAGKGADPYQKVRGVNTPWPTDMAVVKQVAKSLQEG

Specific function: Catalyzes the addition of an amino acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=485, Percent_Identity=25.7731958762887, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_LACC3 (Q03CI6)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805528.1
- STRING:   Q03CI6
- GeneID:   4419225
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0223
- eggNOG:   COG0769
- HOGENOM:   HBG351099
- OMA:   WINATNI
- ProtClustDB:   PRK00139
- BioCyc:   LCAS321967:LSEI_0223-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: 6.3.2.13 [C]

Molecular weight: Translated: 56571; Mature: 56440

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: BINDING 38-38 BINDING 160-160 BINDING 189-189 BINDING 197-197

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSLRACILLLKEHHLLKSAAIQNTEDVDMTGIAYDSRKVSGPTLFFCKGDKFRPIYLS
CCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEE
MAKDNGARTYVAEKPYVEGNGLNALIVRNITKAMAILSAAFFDYPQDDLFVIAYTGTKGK
EECCCCCEEEEECCCEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TTASYFTEAILNEARPRHIALFSTIDTVVGPEPDQRFKSNLTTPESLDLFRDMREAVENG
CHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCC
MTHLVMEVSSQAYLRNRVFGLTYDVGFFLNITPDHIGPNEHPTFANYLHNKLQLLVNARK
HHEEEHHHHHHHHHHHCEEEEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEE
VVINAETEHFDQVYAAATTTTYPESIYLFASAGFRPKRDDIDIDFRFDSQEADVAESRFT
EEEECCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHCCEE
LVPVTEKAAALNIGGHYSLALIGDFNESNATAAIIGAGLAGVDASAAVPGIAKVKIPGRM
EEEECCCCEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
EHTKVAGHGAVYVDYAHNYASMKALLSFLKHAYKDPRLLVVVGSPGDKGVSRRPGFAKVL
CCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
TEFATEAFLTTDDPGYEDPANIIEEIDSKIDHSKVKVHKVLDRKKAIAEAISDSGPNDIV
HHHHHHHEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VVAGKGADPYQKVRGVNTPWPTDMAVVKQVAKSLQEG
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALSLRACILLLKEHHLLKSAAIQNTEDVDMTGIAYDSRKVSGPTLFFCKGDKFRPIYLS
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCEEEEE
MAKDNGARTYVAEKPYVEGNGLNALIVRNITKAMAILSAAFFDYPQDDLFVIAYTGTKGK
EECCCCCEEEEECCCEECCCCCCEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
TTASYFTEAILNEARPRHIALFSTIDTVVGPEPDQRFKSNLTTPESLDLFRDMREAVENG
CHHHHHHHHHHHCCCCCEEEEEEHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHHHCC
MTHLVMEVSSQAYLRNRVFGLTYDVGFFLNITPDHIGPNEHPTFANYLHNKLQLLVNARK
HHEEEHHHHHHHHHHHCEEEEEEECEEEEEECCCCCCCCCCCCHHHHHHHHHHHHEEEEE
VVINAETEHFDQVYAAATTTTYPESIYLFASAGFRPKRDDIDIDFRFDSQEADVAESRFT
EEEECCHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCCCCHHHCCEE
LVPVTEKAAALNIGGHYSLALIGDFNESNATAAIIGAGLAGVDASAAVPGIAKVKIPGRM
EEEECCCCEEEECCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCC
EHTKVAGHGAVYVDYAHNYASMKALLSFLKHAYKDPRLLVVVGSPGDKGVSRRPGFAKVL
CCEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
TEFATEAFLTTDDPGYEDPANIIEEIDSKIDHSKVKVHKVLDRKKAIAEAISDSGPNDIV
HHHHHHHEEECCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VVAGKGADPYQKVRGVNTPWPTDMAVVKQVAKSLQEG
EEECCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10 {D-allo-cystathionine}} 3.9 {L-allo-cystathionine}} 0.62 {ATP}} 0.076 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.035 {UDP-N-MurNAc-L-Ala-D-Glu}} 0.04 {meso-2,6-diaminoheptanedioate}} 0.036 {meso-2,6-diaminoheptanedioate}} 0.097 {dihydrouridine-5´-dip

Substrates: meso-2,6-Diaminoheptanedioate; ATP; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate [C]

Specific reaction: meso-2,6-Diaminoheptanedioate + ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate --> ADP + H+ + Phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diaminopimelate [C]

General reaction: Carboxamide formation; Carboxylic acid amide formation [C]

Inhibitor: MurNAc (alpha-methyl)-L-Ala-D-Glu; MurNAc (beta-methyl)-L-Ala-D-Glu; N alpha-Propionyl -L-Ala-2-amino-6-phosphonohexanoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA