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The map label for this gene is dnaN [H]

Identifier: 116493576

GI number: 116493576

Start: 1603

End: 2742

Strand: Direct

Name: dnaN [H]

Synonym: LSEI_0002

Alternate gene names: 116493576

Gene position: 1603-2742 (Clockwise)

Preceding gene: 116493575

Following gene: 116493577

Centisome position: 0.06

GC content: 44.65

Gene sequence:

>1140_bases
ATGAAATTTACGATCACCCGATCCACATTCTTAAAAACCTTGAATGATGTTTCTCGGGCCATTTCAACCAAAACAACCAT
TCCGATTCTGACTGGATTGAAAATTGTGCTCAACGATAGCGGCTTAATCCTCACTGGTAGTGATGCCGATATTTCAATCG
AATCACGGATCAACGCAACGGATGAAAATAATGATCTCCAAATCGGAAGCACTGGTGAAATCGTCTTGCCAGCCCGTTTC
TTTAGCGAAATCGTTAAACGTTTGCCAGAAAGCACAATGACGCTTGAAGTTAAAGACAATTTCCAAACCGTCATCACGTC
TGGGGCATCTGAATTTACCATTAATGGTTTGGATGCCAATAACTATCCGCGGTTGCCAGAAATTACAGCCGATGCAGCGT
TGAGTGTTTCAGCTGATGTGTTACGTCAGTTGATTAACCAAACAGTTATTGCCGTTTCTAATCAGGAAAGTCGACCAATC
TTGACAGGGGTTCATCTGACAATTACCGGCGATCAGTTGGTAGCTGTTGCGACTGATTCCCACCGCCTTGCGCAACGTAG
CCTGACCTTACCAACAGCAAGTGCCTCGGATTATGACATCATTATTCCAGGGAAAAGTCTGACAGAACTTAGCCGCATGT
TAAGCGATGACGTTGAAAAAATCGAAATTCGCATTGCGGAAAATCAAGTGCTGTTCGTGTTTGGGCAAACGGCCTTTTAT
TCACGCTTACTTGAAGGCAACTACCCTGACACAAGCCGACTTATTCCAACATCCAGCAATACGCAAGCGGAATTTGACGC
ACCAGCATTGCTGGCCTCCATTCAACGTGCCAGCTTGCTGAGTCACGAAAGTAGCAACAATGTAGTTCGGCTTGTCCTTA
ATATCGCTGATCAAAAAGCGACTATCTACGGTAATTCGCCAGATGTCGGGAATGTTGAAGAAGTACTTAGTTTCAACAAA
CTTAGCGGCGAGGATCTAGAAATCTCGTTCAACCCTGATTATATGAAGGATGCCTTGCAAGGATTTGGCCAAACGGCAAT
TGAAGTTGACTTCACCGCACCATTGCGACCATTCACTTTGGTTCCAACAGAAGACAAGGAACGGTTTATCCAACTCATCA
CGCCAGTTCGAACGTTCTAA

Upstream 100 bases:

>100_bases
AGTTGTTGTTTCTCAAAGTTTTCCACAGTTTGAACACGCCCTATTACTATTACTTCAAAAGCTTTATATTATATATATAA
AAGACGTTCAGGAGGCTCTT

Downstream 100 bases:

>100_bases
CTAAACATGACTTGAAAACGCCTTTACCGAAAAGATTCGGTGAGGCGTTTTTTTGACGAACTTATGCTTCATGAAATGAA
CTGATAATGTGCGCATCAAA

Product: DNA polymerase sliding clamp subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MKFTITRSTFLKTLNDVSRAISTKTTIPILTGLKIVLNDSGLILTGSDADISIESRINATDENNDLQIGSTGEIVLPARF
FSEIVKRLPESTMTLEVKDNFQTVITSGASEFTINGLDANNYPRLPEITADAALSVSADVLRQLINQTVIAVSNQESRPI
LTGVHLTITGDQLVAVATDSHRLAQRSLTLPTASASDYDIIIPGKSLTELSRMLSDDVEKIEIRIAENQVLFVFGQTAFY
SRLLEGNYPDTSRLIPTSSNTQAEFDAPALLASIQRASLLSHESSNNVVRLVLNIADQKATIYGNSPDVGNVEEVLSFNK
LSGEDLEISFNPDYMKDALQGFGQTAIEVDFTAPLRPFTLVPTEDKERFIQLITPVRTF

Sequences:

>Translated_379_residues
MKFTITRSTFLKTLNDVSRAISTKTTIPILTGLKIVLNDSGLILTGSDADISIESRINATDENNDLQIGSTGEIVLPARF
FSEIVKRLPESTMTLEVKDNFQTVITSGASEFTINGLDANNYPRLPEITADAALSVSADVLRQLINQTVIAVSNQESRPI
LTGVHLTITGDQLVAVATDSHRLAQRSLTLPTASASDYDIIIPGKSLTELSRMLSDDVEKIEIRIAENQVLFVFGQTAFY
SRLLEGNYPDTSRLIPTSSNTQAEFDAPALLASIQRASLLSHESSNNVVRLVLNIADQKATIYGNSPDVGNVEEVLSFNK
LSGEDLEISFNPDYMKDALQGFGQTAIEVDFTAPLRPFTLVPTEDKERFIQLITPVRTF
>Mature_379_residues
MKFTITRSTFLKTLNDVSRAISTKTTIPILTGLKIVLNDSGLILTGSDADISIESRINATDENNDLQIGSTGEIVLPARF
FSEIVKRLPESTMTLEVKDNFQTVITSGASEFTINGLDANNYPRLPEITADAALSVSADVLRQLINQTVIAVSNQESRPI
LTGVHLTITGDQLVAVATDSHRLAQRSLTLPTASASDYDIIIPGKSLTELSRMLSDDVEKIEIRIAENQVLFVFGQTAFY
SRLLEGNYPDTSRLIPTSSNTQAEFDAPALLASIQRASLLSHESSNNVVRLVLNIADQKATIYGNSPDVGNVEEVLSFNK
LSGEDLEISFNPDYMKDALQGFGQTAIEVDFTAPLRPFTLVPTEDKERFIQLITPVRTF

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=339, Percent_Identity=30.0884955752212, Blast_Score=162, Evalue=3e-41,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41457; Mature: 41457

Theoretical pI: Translated: 4.39; Mature: 4.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFTITRSTFLKTLNDVSRAISTKTTIPILTGLKIVLNDSGLILTGSDADISIESRINAT
CEEEEEHHHHHHHHHHHHHHHHCCCCEEHHCCEEEEEECCCEEEECCCCCEEEECCCCCC
DENNDLQIGSTGEIVLPARFFSEIVKRLPESTMTLEVKDNFQTVITSGASEFTINGLDAN
CCCCCEEECCCCCEEECHHHHHHHHHHCCCCEEEEEECCCCEEEHCCCCCEEEEECCCCC
NYPRLPEITADAALSVSADVLRQLINQTVIAVSNQESRPILTGVHLTITGDQLVAVATDS
CCCCCCCCCCCHHHCCCHHHHHHHHCCEEEEECCCCCCCEEEEEEEEEECCEEEEEECCC
HRLAQRSLTLPTASASDYDIIIPGKSLTELSRMLSDDVEKIEIRIAENQVLFVFGQTAFY
HHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEEECCEEEEEEECHHHH
SRLLEGNYPDTSRLIPTSSNTQAEFDAPALLASIQRASLLSHESSNNVVRLVLNIADQKA
HHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEE
TIYGNSPDVGNVEEVLSFNKLSGEDLEISFNPDYMKDALQGFGQTAIEVDFTAPLRPFTL
EEECCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCEEE
VPTEDKERFIQLITPVRTF
CCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKFTITRSTFLKTLNDVSRAISTKTTIPILTGLKIVLNDSGLILTGSDADISIESRINAT
CEEEEEHHHHHHHHHHHHHHHHCCCCEEHHCCEEEEEECCCEEEECCCCCEEEECCCCCC
DENNDLQIGSTGEIVLPARFFSEIVKRLPESTMTLEVKDNFQTVITSGASEFTINGLDAN
CCCCCEEECCCCCEEECHHHHHHHHHHCCCCEEEEEECCCCEEEHCCCCCEEEEECCCCC
NYPRLPEITADAALSVSADVLRQLINQTVIAVSNQESRPILTGVHLTITGDQLVAVATDS
CCCCCCCCCCCHHHCCCHHHHHHHHCCEEEEECCCCCCCEEEEEEEEEECCEEEEEECCC
HRLAQRSLTLPTASASDYDIIIPGKSLTELSRMLSDDVEKIEIRIAENQVLFVFGQTAFY
HHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEEEEECCEEEEEEECHHHH
SRLLEGNYPDTSRLIPTSSNTQAEFDAPALLASIQRASLLSHESSNNVVRLVLNIADQKA
HHHHCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCEE
TIYGNSPDVGNVEEVLSFNKLSGEDLEISFNPDYMKDALQGFGQTAIEVDFTAPLRPFTL
EEECCCCCCCCHHHHHHHCCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEECCCCCCEEE
VPTEDKERFIQLITPVRTF
CCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377; 2846289 [H]