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The map label for this gene is dnaA

Identifier: 116493575

GI number: 116493575

Start: 81

End: 1430

Strand: Direct

Name: dnaA

Synonym: LSEI_0001

Alternate gene names: 116493575

Gene position: 81-1430 (Clockwise)

Preceding gene: NA

Following gene: 116493576

Centisome position: 0.0

GC content: 43.85

Gene sequence:

>1350_bases
ATGCCCAATTTAGAGGAACTTTGGGCTTACCTGAATGATAAATTCCGTGAAGAATTGACCCCGGTCGGTTACAGCACATG
GATCCAGACAGCAAAACCCGTCAAATTAACCAAAGATAAACTCGAAATTGAAGTCCCAGCTTCTTTACACAAAGCTTATT
GGGAAAAGAATCTCGTGACCAAGGTAGTCGAAGGCGTTTACGAATTCGCGCAACTTGAAGTTGATCCCGTCATTATGACC
AAGGATGAGTTGCAACCAGCTCCGGCTACAGATCAACGTCCTGCGGTTGAAGAAGACGATCAAAATCTGACTTTTAAGGC
AAAAACCCATTTAAATCCGAAATATACGTTTGACCACTTCGTGATTGGTAAGGGAAACCAAATGGCCCACGCGGCTGCTT
TGGCGGTTGCTGAAGCCCCTGGGACAACATACAACCCTCTGTTTATTTATGGTGGCGTTGGTCTTGGCAAAACCCACTTA
ATGCAAGCGATTGGTAATTTGGTGCTAGGTAATAATCCAGCAGCCAACATAAAATACGTCACCAGTGAGAATTTTGCTAA
TGACTTCATTAACTCGATTCAAACCAAACAACAGGAACAATTTCGGCAAGAATACCGAAATGTGGATCTGCTGTTGGTAG
ATGATATTCAGTTTTTTGGTGACAAAGAGGCCACACAGGAAGAATTTTTTCATACCTTCAATACGCTGTATGAAAATATG
AAGCAAATTGTTTTGACCAGTGATCGCCTGCCTAACGAAATCCCCAAACTTCAAGAACGGTTGGTTTCGCGGTTTAATAA
GGGCTTGTCTGTCGATGTCACACCGCCAGATCTGGAAACACGAATCGCTATTTTGCGGAATAAAGCCGATGCTGAAGATC
TCAGCATTCCAGATGACACCTTGTCTTACATTGCTGGTCAAATTGAAAGTAACGTACGTGATCTCGAAGGTGCGTTAGTA
CGCGTTCAAGCCTTTTCAACCATGAAAAACGAAGATATCACGACCAGCTTAGCCGCGGATGCCTTGAAAGCCCTGAAACT
TGATGACCGCAGCGGACAATTGACCATTCCGCAAATCCTGAATGCTGTCGCGAAATATTTTCAGCTGACGGTTCAGGATC
TTAAAGGAAAGAAACGCGTCAAACAGATTGTCATTCCGCGCCAAATCGCGATGTATCTAGCGCGCGAAATGACCGATAAC
AGTTTACCGAAAATAGGTCAGGAAATCGGCGGCAAGGATCATACAACCGTCATCCATGCCCACGAAAAAATTATGGCTTC
AATGACAACTGATGAGAATTTAAAAGCTCAAGTGATCGAATTGCGGAATATCTTGAAAAACCGCGGGTAA

Upstream 100 bases:

>100_bases
GGTGCCAATGAGCGCGTTTTTATATAGGAATGTCCGTCATCACTCGTCAGCATAGCTATAAGCTGGTTTGCTACCGGACA
TAATTGGAGGAGGGGCATGA

Downstream 100 bases:

>100_bases
TGCACATGTGGATAACCTTCAAAAACAAACCATTTTATCCGTCGCACTTATCCACAAGTGCATAACTTTCTCAGTTGTTG
TTTCTCAAAGTTTTCCACAG

Product: chromosomal replication initiation protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MPNLEELWAYLNDKFREELTPVGYSTWIQTAKPVKLTKDKLEIEVPASLHKAYWEKNLVTKVVEGVYEFAQLEVDPVIMT
KDELQPAPATDQRPAVEEDDQNLTFKAKTHLNPKYTFDHFVIGKGNQMAHAAALAVAEAPGTTYNPLFIYGGVGLGKTHL
MQAIGNLVLGNNPAANIKYVTSENFANDFINSIQTKQQEQFRQEYRNVDLLLVDDIQFFGDKEATQEEFFHTFNTLYENM
KQIVLTSDRLPNEIPKLQERLVSRFNKGLSVDVTPPDLETRIAILRNKADAEDLSIPDDTLSYIAGQIESNVRDLEGALV
RVQAFSTMKNEDITTSLAADALKALKLDDRSGQLTIPQILNAVAKYFQLTVQDLKGKKRVKQIVIPRQIAMYLAREMTDN
SLPKIGQEIGGKDHTTVIHAHEKIMASMTTDENLKAQVIELRNILKNRG

Sequences:

>Translated_449_residues
MPNLEELWAYLNDKFREELTPVGYSTWIQTAKPVKLTKDKLEIEVPASLHKAYWEKNLVTKVVEGVYEFAQLEVDPVIMT
KDELQPAPATDQRPAVEEDDQNLTFKAKTHLNPKYTFDHFVIGKGNQMAHAAALAVAEAPGTTYNPLFIYGGVGLGKTHL
MQAIGNLVLGNNPAANIKYVTSENFANDFINSIQTKQQEQFRQEYRNVDLLLVDDIQFFGDKEATQEEFFHTFNTLYENM
KQIVLTSDRLPNEIPKLQERLVSRFNKGLSVDVTPPDLETRIAILRNKADAEDLSIPDDTLSYIAGQIESNVRDLEGALV
RVQAFSTMKNEDITTSLAADALKALKLDDRSGQLTIPQILNAVAKYFQLTVQDLKGKKRVKQIVIPRQIAMYLAREMTDN
SLPKIGQEIGGKDHTTVIHAHEKIMASMTTDENLKAQVIELRNILKNRG
>Mature_448_residues
PNLEELWAYLNDKFREELTPVGYSTWIQTAKPVKLTKDKLEIEVPASLHKAYWEKNLVTKVVEGVYEFAQLEVDPVIMTK
DELQPAPATDQRPAVEEDDQNLTFKAKTHLNPKYTFDHFVIGKGNQMAHAAALAVAEAPGTTYNPLFIYGGVGLGKTHLM
QAIGNLVLGNNPAANIKYVTSENFANDFINSIQTKQQEQFRQEYRNVDLLLVDDIQFFGDKEATQEEFFHTFNTLYENMK
QIVLTSDRLPNEIPKLQERLVSRFNKGLSVDVTPPDLETRIAILRNKADAEDLSIPDDTLSYIAGQIESNVRDLEGALVR
VQAFSTMKNEDITTSLAADALKALKLDDRSGQLTIPQILNAVAKYFQLTVQDLKGKKRVKQIVIPRQIAMYLAREMTDNS
LPKIGQEIGGKDHTTVIHAHEKIMASMTTDENLKAQVIELRNILKNRG

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family

Homologues:

Organism=Escherichia coli, GI2367267, Length=317, Percent_Identity=50.4731861198738, Blast_Score=330, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAA_LACC3 (Q03D55)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805309.1
- ProteinModelPortal:   Q03D55
- SMR:   Q03D55
- STRING:   Q03D55
- GeneID:   4421687
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0001
- eggNOG:   COG0593
- HOGENOM:   HBG695666
- OMA:   DEVAFLI
- PhylomeDB:   Q03D55
- ProtClustDB:   PRK00149
- BioCyc:   LCAS321967:LSEI_0001-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00377
- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921
- Gene3D:   G3DSA:1.10.1750.10
- PRINTS:   PR00051
- SMART:   SM00382
- SMART:   SM00760
- TIGRFAMs:   TIGR00362

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C; SSF48295 Trp_repress_rep

EC number: NA

Molecular weight: Translated: 50614; Mature: 50483

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNLEELWAYLNDKFREELTPVGYSTWIQTAKPVKLTKDKLEIEVPASLHKAYWEKNLVT
CCCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEECCCCEEEECCHHHHHHHHHHHHHH
KVVEGVYEFAQLEVDPVIMTKDELQPAPATDQRPAVEEDDQNLTFKAKTHLNPKYTFDHF
HHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECEE
VIGKGNQMAHAAALAVAEAPGTTYNPLFIYGGVGLGKTHLMQAIGNLVLGNNPAANIKYV
EEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHEEECCCCCCCEEEE
TSENFANDFINSIQTKQQEQFRQEYRNVDLLLVDDIQFFGDKEATQEEFFHTFNTLYENM
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHH
KQIVLTSDRLPNEIPKLQERLVSRFNKGLSVDVTPPDLETRIAILRNKADAEDLSIPDDT
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHH
LSYIAGQIESNVRDLEGALVRVQAFSTMKNEDITTSLAADALKALKLDDRSGQLTIPQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHH
NAVAKYFQLTVQDLKGKKRVKQIVIPRQIAMYLAREMTDNSLPKIGQEIGGKDHTTVIHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEH
HEKIMASMTTDENLKAQVIELRNILKNRG
HHHHHHHCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNLEELWAYLNDKFREELTPVGYSTWIQTAKPVKLTKDKLEIEVPASLHKAYWEKNLVT
CCHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCEECCCCEEEECCHHHHHHHHHHHHHH
KVVEGVYEFAQLEVDPVIMTKDELQPAPATDQRPAVEEDDQNLTFKAKTHLNPKYTFDHF
HHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECEE
VIGKGNQMAHAAALAVAEAPGTTYNPLFIYGGVGLGKTHLMQAIGNLVLGNNPAANIKYV
EEECCCCHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHEEECCCCCCCEEEE
TSENFANDFINSIQTKQQEQFRQEYRNVDLLLVDDIQFFGDKEATQEEFFHTFNTLYENM
ECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHCCCCHHHHHHHHHHHHHHHHH
KQIVLTSDRLPNEIPKLQERLVSRFNKGLSVDVTPPDLETRIAILRNKADAEDLSIPDDT
HHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCCCCCCCHHH
LSYIAGQIESNVRDLEGALVRVQAFSTMKNEDITTSLAADALKALKLDDRSGQLTIPQIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHH
NAVAKYFQLTVQDLKGKKRVKQIVIPRQIAMYLAREMTDNSLPKIGQEIGGKDHTTVIHA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEH
HEKIMASMTTDENLKAQVIELRNILKNRG
HHHHHHHCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA