Click here to switch to the map view.

The map label for this gene is mscL

Identifier: 116493538

GI number: 116493538

Start: 1791361

End: 1791783

Strand: Direct

Name: mscL

Synonym: PEPE_1812

Alternate gene names: 116493538

Gene position: 1791361-1791783 (Clockwise)

Preceding gene: 116493537

Following gene: 116493540

Centisome position: 97.76

GC content: 30.26

Gene sequence:

>423_bases
TTGCTTAAAGAATTTAAAGAATTTGTTTCACGTGGAAACGTCCTTGATTTAGCTGTCGGTGTTATTATTGGTGGAGCTTT
TACTTCCATCGTTAAATCATTAGTTAATTATTTAATTAATCCTTTAATTGGTTTATTTATCGGCGGAATTGATTTTTCAG
ATTGGGTACTTAAGGTAGCCGGAGCTACTTTCAAGTTTGGTAGTTTTATCAATGCTGTCATTAACTTCTTAATCATTGCA
TTTGTTGTCTTTATTTTGGTTAAATTAGTCAACAAGTTTCTTCCTAAGAAAGAAGAACAAGAAGACGATGAAGCTGAAGC
AATTGAAAATAGTGAAATTTACCTTAAAGAAATTCGCGATGCATTAGTAAATAATCCTGAATTACTTTCCAACTTAAAAA
ACAAAGAAGATTCAACTAAATAA

Upstream 100 bases:

>100_bases
AAATTACAGTTCCCTCACTTAGGTAAGTTTTGATAACAGTTCGATTCTGTTAGAGGGATTCAGAGAATTAATCTCTAAAA
AAAACTAGGAGTTGGTTGAT

Downstream 100 bases:

>100_bases
CCTAAAAAACGTTTCTTGAGAGTTTTCTCAAGAAACGTTTTTTTACTAAAGTTATTCAGTTCTTAAAGCAATTGCTGCAT
CTTTTTTAGCAGCCATTCTA

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 140

Protein sequence:

>140_residues
MLKEFKEFVSRGNVLDLAVGVIIGGAFTSIVKSLVNYLINPLIGLFIGGIDFSDWVLKVAGATFKFGSFINAVINFLIIA
FVVFILVKLVNKFLPKKEEQEDDEAEAIENSEIYLKEIRDALVNNPELLSNLKNKEDSTK

Sequences:

>Translated_140_residues
MLKEFKEFVSRGNVLDLAVGVIIGGAFTSIVKSLVNYLINPLIGLFIGGIDFSDWVLKVAGATFKFGSFINAVINFLIIA
FVVFILVKLVNKFLPKKEEQEDDEAEAIENSEIYLKEIRDALVNNPELLSNLKNKEDSTK
>Mature_140_residues
MLKEFKEFVSRGNVLDLAVGVIIGGAFTSIVKSLVNYLINPLIGLFIGGIDFSDWVLKVAGATFKFGSFINAVINFLIIA
FVVFILVKLVNKFLPKKEEQEDDEAEAIENSEIYLKEIRDALVNNPELLSNLKNKEDSTK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=129, Percent_Identity=48.8372093023256, Blast_Score=108, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_PEDPA (Q03D91)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805273.1
- ProteinModelPortal:   Q03D91
- SMR:   Q03D91
- STRING:   Q03D91
- GeneID:   4417358
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1812
- NMPDR:   fig|278197.10.peg.1604
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   FATITKS
- PhylomeDB:   Q03D91
- BioCyc:   PPEN278197:PEPE_1812-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15598; Mature: 15598

Theoretical pI: Translated: 4.62; Mature: 4.62

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x18c2ab1c)-; HASH(0x19176b48)-; HASH(0x1919c5c0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKEFKEFVSRGNVLDLAVGVIIGGAFTSIVKSLVNYLINPLIGLFIGGIDFSDWVLKVA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GATFKFGSFINAVINFLIIAFVVFILVKLVNKFLPKKEEQEDDEAEAIENSEIYLKEIRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHH
ALVNNPELLSNLKNKEDSTK
HHCCCHHHHHHHHCCCCCCH
>Mature Secondary Structure
MLKEFKEFVSRGNVLDLAVGVIIGGAFTSIVKSLVNYLINPLIGLFIGGIDFSDWVLKVA
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GATFKFGSFINAVINFLIIAFVVFILVKLVNKFLPKKEEQEDDEAEAIENSEIYLKEIRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHH
ALVNNPELLSNLKNKEDSTK
HHCCCHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA