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The map label for this gene is yxjB [H]

Identifier: 116493512

GI number: 116493512

Start: 1760773

End: 1761612

Strand: Direct

Name: yxjB [H]

Synonym: PEPE_1784

Alternate gene names: 116493512

Gene position: 1760773-1761612 (Clockwise)

Preceding gene: 116493505

Following gene: 116493516

Centisome position: 96.09

GC content: 35.83

Gene sequence:

>840_bases
TTGAATAAAATCGAATTAGCAACCCAATTTATCCAACGACACTTACACCAATTTCAGTGTCCCATCTGTAAATCGAATTT
CAATGAAGTTCAATCCGGGACTTTAATTTGTAATAATCAGCATTCATTTAATATTGCCAAAAAAGGAAGTCTCTTCTTTT
TAAATAATCCTGTTAACACTGAATATACTAATGAGATGTTAACTTATCGAAATCAGTTTTTGACGGCTGGTTTTTTCAAC
CCGATGTTAGAAGAAGTTATCAATAATATTTCGGGAGATTTAATTTTAGATGCAGGTTGTGGAGAAGGAACTACTACTAA
ATGGATGGCATCTCATTTCGACGGAGACTTCGTGGGTTTGGATATCTCTAAACCAGCGATTAATATTGCCGGCTCTGGAA
TTGCTCCTCAACCCTATCCTTTATTTATGGTGGGCGATTTAGCTCAATTACCTTTCGCAGATCAAGGTTTAACTTCAATC
GTCAACATTTTATCTCCAGCCAATTATCAAGAATTTGATCGAACGCTGGCAACCCATGGTGAATTAATTAAAGTGATTCC
TGGAAATCATTATCTAAGTGAACTTCGACAACTCGTCTACCCTGATGGTGAACATGCGACCTATGATAATGCTCCGGTTA
AAAACCATTTTATCGAACACTATGAACACGTCGAATTTTCTAAAATCAGCTATAAATTTCCGCTTAATGATAAATTATTC
ACGGCTCTTTTCAATATGACACCTTTAACTTGGCAAGCTCAGGATCGTAAAGAGGCTATTTTACAAGATGGGTTAAAAGA
AATCACGGCGGAATTTGAAATTGGTGTAGTTCATAAATAA

Upstream 100 bases:

>100_bases
ACTAGCTTCTTTTCGTTACATTAAACTAACGATTGAAACAATCCGGTGGTATTAAATGGTAGAATAGTTTTAATATCTTT
CAGAATATCGAGGTTTCATC

Downstream 100 bases:

>100_bases
TAAAAAGGCTTCAATCAATGTTAACGAATTGATTGAAGCCTTTTTATATATTATTAATATTTTAAATTTATTGTAGTTTT
TGAATGTACTTGGATTAAGT

Product: SAM-dependent methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNKIELATQFIQRHLHQFQCPICKSNFNEVQSGTLICNNQHSFNIAKKGSLFFLNNPVNTEYTNEMLTYRNQFLTAGFFN
PMLEEVINNISGDLILDAGCGEGTTTKWMASHFDGDFVGLDISKPAINIAGSGIAPQPYPLFMVGDLAQLPFADQGLTSI
VNILSPANYQEFDRTLATHGELIKVIPGNHYLSELRQLVYPDGEHATYDNAPVKNHFIEHYEHVEFSKISYKFPLNDKLF
TALFNMTPLTWQAQDRKEAILQDGLKEITAEFEIGVVHK

Sequences:

>Translated_279_residues
MNKIELATQFIQRHLHQFQCPICKSNFNEVQSGTLICNNQHSFNIAKKGSLFFLNNPVNTEYTNEMLTYRNQFLTAGFFN
PMLEEVINNISGDLILDAGCGEGTTTKWMASHFDGDFVGLDISKPAINIAGSGIAPQPYPLFMVGDLAQLPFADQGLTSI
VNILSPANYQEFDRTLATHGELIKVIPGNHYLSELRQLVYPDGEHATYDNAPVKNHFIEHYEHVEFSKISYKFPLNDKLF
TALFNMTPLTWQAQDRKEAILQDGLKEITAEFEIGVVHK
>Mature_279_residues
MNKIELATQFIQRHLHQFQCPICKSNFNEVQSGTLICNNQHSFNIAKKGSLFFLNNPVNTEYTNEMLTYRNQFLTAGFFN
PMLEEVINNISGDLILDAGCGEGTTTKWMASHFDGDFVGLDISKPAINIAGSGIAPQPYPLFMVGDLAQLPFADQGLTSI
VNILSPANYQEFDRTLATHGELIKVIPGNHYLSELRQLVYPDGEHATYDNAPVKNHFIEHYEHVEFSKISYKFPLNDKLF
TALFNMTPLTWQAQDRKEAILQDGLKEITAEFEIGVVHK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=250, Percent_Identity=29.2, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 31477; Mature: 31477

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIELATQFIQRHLHQFQCPICKSNFNEVQSGTLICNNQHSFNIAKKGSLFFLNNPVNT
CCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEECCCCCEEEEECCCEEEECCCCCC
EYTNEMLTYRNQFLTAGFFNPMLEEVINNISGDLILDAGCGEGTTTKWMASHFDGDFVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEE
DISKPAINIAGSGIAPQPYPLFMVGDLAQLPFADQGLTSIVNILSPANYQEFDRTLATHG
ECCCCCEEECCCCCCCCCCCEEEECCHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
ELIKVIPGNHYLSELRQLVYPDGEHATYDNAPVKNHFIEHYEHVEFSKISYKFPLNDKLF
CEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCHHHH
TALFNMTPLTWQAQDRKEAILQDGLKEITAEFEIGVVHK
HHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHEEEEECC
>Mature Secondary Structure
MNKIELATQFIQRHLHQFQCPICKSNFNEVQSGTLICNNQHSFNIAKKGSLFFLNNPVNT
CCHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEECCCCCEEEEECCCEEEECCCCCC
EYTNEMLTYRNQFLTAGFFNPMLEEVINNISGDLILDAGCGEGTTTKWMASHFDGDFVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHCCCCEEEE
DISKPAINIAGSGIAPQPYPLFMVGDLAQLPFADQGLTSIVNILSPANYQEFDRTLATHG
ECCCCCEEECCCCCCCCCCCEEEECCHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCC
ELIKVIPGNHYLSELRQLVYPDGEHATYDNAPVKNHFIEHYEHVEFSKISYKFPLNDKLF
CEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCEEEEEEEECCCCHHHH
TALFNMTPLTWQAQDRKEAILQDGLKEITAEFEIGVVHK
HHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]