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The map label for this gene is birA [H]

Identifier: 116493364

GI number: 116493364

Start: 1620259

End: 1621242

Strand: Reverse

Name: birA [H]

Synonym: PEPE_1636

Alternate gene names: 116493364

Gene position: 1621242-1620259 (Counterclockwise)

Preceding gene: 116493365

Following gene: 116493363

Centisome position: 88.48

GC content: 37.2

Gene sequence:

>984_bases
ATGGGGAATAAAACAAGCCAAAAAGTTTTATTAGAATTATTGAAAGATCGAGATCGTTGGGTATCTGGAAATGAAATAGC
AGATAGTCTAGGCGTTACCCGCGAAACGATTTGGAAAAATATTAACCTATTAAAGAAACGCGGTCACCAAATCCAAAGTC
AGAAAAATCTAGGTTACAAATATGAAAGTAGCTCTAAGTTGGATGAAGTAGTCATTAATTTTTATAAAAATAAAAGTGTT
GATCATGTTGCTGTTGAAGAGCAAGTCGATTCAACTCAAATGTATGCTAAAAACTATCTCGCTACTCACCAAGTCGAAAA
GGCATTTGCTGTGATTAGCGAGCAGCAAACGCAAGGATACGGACGAAGAGGACGTAGTTTTTACTCACCCAAAGAACAAG
GACTTTACTTTAGCATAGTTATCCCTAAGCCCACTTTTGAAATTAACCAGGTGGGCTTAATAACTACTGGGGTTGCCTAT
GTGATTGCACAAACATTTAAAGATTTCTTCCCTGAACGTGAATTTAAATTGAAATGGGTTAACGACATTTTAATGAATCG
ACGAAAGGTGGCAGGAATTATTACTGAGGCAATTACCGAAGTAGAATCTGCTTCTACGCAAGCGTTTGTAATCGGGGTGG
GAATTAACTTAACCACGCCTGAATTTCCAGAAGAATTACAACAAAAAGCGGGAGCCATTGTTGCTAAAAATGAACCGAAC
ATTGATCGTAATTTAATCGCCGCCCAATTAATTAATACGATTGTAGATAATTGGCAAAATTATTTAGATGGCAGCTTTTT
GTCCGAGTATCGAAGAATGTCAATCGTAATTGGTAAGCCAGTTACCTTACAAGTCGGAACGAAACAAGTAAAGGGGATTG
CAACGGATATTAACGAGTATGGAAATATCGTCGTAAAGTTGGATGATGGACAAGTTAAAGCTTTTAATAACGGTGAAATT
ACCAAGGTGAATTTGGACTGGTAA

Upstream 100 bases:

>100_bases
CAGGTGATACAATCAAAGCTATTTTAGTAATTATCATAGCCAATCGATTGGAGAAGATAGTACACTTAAACTAGTTAAAT
AGATTAAGGGTGGTTATAAG

Downstream 100 bases:

>100_bases
TTATGGTCTCCGTAAGATATAGGTAATGAGCTTGAGCTTTTTTATTCATTGAGTGTTATCATGAAAGAATGTATCAGATA
ACGAAGGAGATGAATTACAA

Product: biotin operon repressor

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MGNKTSQKVLLELLKDRDRWVSGNEIADSLGVTRETIWKNINLLKKRGHQIQSQKNLGYKYESSSKLDEVVINFYKNKSV
DHVAVEEQVDSTQMYAKNYLATHQVEKAFAVISEQQTQGYGRRGRSFYSPKEQGLYFSIVIPKPTFEINQVGLITTGVAY
VIAQTFKDFFPEREFKLKWVNDILMNRRKVAGIITEAITEVESASTQAFVIGVGINLTTPEFPEELQQKAGAIVAKNEPN
IDRNLIAAQLINTIVDNWQNYLDGSFLSEYRRMSIVIGKPVTLQVGTKQVKGIATDINEYGNIVVKLDDGQVKAFNNGEI
TKVNLDW

Sequences:

>Translated_327_residues
MGNKTSQKVLLELLKDRDRWVSGNEIADSLGVTRETIWKNINLLKKRGHQIQSQKNLGYKYESSSKLDEVVINFYKNKSV
DHVAVEEQVDSTQMYAKNYLATHQVEKAFAVISEQQTQGYGRRGRSFYSPKEQGLYFSIVIPKPTFEINQVGLITTGVAY
VIAQTFKDFFPEREFKLKWVNDILMNRRKVAGIITEAITEVESASTQAFVIGVGINLTTPEFPEELQQKAGAIVAKNEPN
IDRNLIAAQLINTIVDNWQNYLDGSFLSEYRRMSIVIGKPVTLQVGTKQVKGIATDINEYGNIVVKLDDGQVKAFNNGEI
TKVNLDW
>Mature_326_residues
GNKTSQKVLLELLKDRDRWVSGNEIADSLGVTRETIWKNINLLKKRGHQIQSQKNLGYKYESSSKLDEVVINFYKNKSVD
HVAVEEQVDSTQMYAKNYLATHQVEKAFAVISEQQTQGYGRRGRSFYSPKEQGLYFSIVIPKPTFEINQVGLITTGVAYV
IAQTFKDFFPEREFKLKWVNDILMNRRKVAGIITEAITEVESASTQAFVIGVGINLTTPEFPEELQQKAGAIVAKNEPNI
DRNLIAAQLINTIVDNWQNYLDGSFLSEYRRMSIVIGKPVTLQVGTKQVKGIATDINEYGNIVVKLDDGQVKAFNNGEIT
KVNLDW

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=303, Percent_Identity=27.3927392739274, Blast_Score=112, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36977; Mature: 36845

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNKTSQKVLLELLKDRDRWVSGNEIADSLGVTRETIWKNINLLKKRGHQIQSQKNLGYK
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCEE
YESSSKLDEVVINFYKNKSVDHVAVEEQVDSTQMYAKNYLATHQVEKAFAVISEQQTQGY
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRRGRSFYSPKEQGLYFSIVIPKPTFEINQVGLITTGVAYVIAQTFKDFFPEREFKLKWV
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHH
NDILMNRRKVAGIITEAITEVESASTQAFVIGVGINLTTPEFPEELQQKAGAIVAKNEPN
HHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCC
IDRNLIAAQLINTIVDNWQNYLDGSFLSEYRRMSIVIGKPVTLQVGTKQVKGIATDINEY
CHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHHHHH
GNIVVKLDDGQVKAFNNGEITKVNLDW
CCEEEEECCCEEEEECCCCEEEEEECC
>Mature Secondary Structure 
GNKTSQKVLLELLKDRDRWVSGNEIADSLGVTRETIWKNINLLKKRGHQIQSQKNLGYK
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHCCCCEE
YESSSKLDEVVINFYKNKSVDHVAVEEQVDSTQMYAKNYLATHQVEKAFAVISEQQTQGY
ECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRRGRSFYSPKEQGLYFSIVIPKPTFEINQVGLITTGVAYVIAQTFKDFFPEREFKLKWV
CCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHH
NDILMNRRKVAGIITEAITEVESASTQAFVIGVGINLTTPEFPEELQQKAGAIVAKNEPN
HHHHHCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHCCEEEECCCCC
IDRNLIAAQLINTIVDNWQNYLDGSFLSEYRRMSIVIGKPVTLQVGTKQVKGIATDINEY
CHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHEEEEECCCEEEEECCHHHHHHHHHHHHH
GNIVVKLDDGQVKAFNNGEITKVNLDW
CCEEEEECCCEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]