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The map label for this gene is mleR [H]

Identifier: 116493346

GI number: 116493346

Start: 1596590

End: 1597477

Strand: Direct

Name: mleR [H]

Synonym: PEPE_1618

Alternate gene names: 116493346

Gene position: 1596590-1597477 (Clockwise)

Preceding gene: 116493342

Following gene: 116493350

Centisome position: 87.13

GC content: 34.8

Gene sequence:

>888_bases
ATGAACATTAAAGATTTGAATTATTTTCATGAACTCGTAAAAAATAAAAATTTTTCTTCAGTTGCCGAAATTTTTCATGT
TAGCCAACCGACAATTAGTATGTCAGTTCGCCGATTAGAAGAAGAGTTTAATACCACCCTTTTCACTCGCGACCAAGGAC
GTCACCACTTAAATGTCACTTTGAGCGGTCAACAACTAGATGAACATGTTAATGCGATTTTGGATCATATCGCCATTGCG
CAAAATAGTATCAACAATATTAATACCAACAAACTCATTTTGGGATTACCCCCCATTATGACCAAATACTTTTTTCCAAA
AATCGCTAAACAGCTTACCGAACAAAATCTATTAAAAGCAATTGTTCCCGTAGAAAAAGGATCAGCTGAGTTAGAAAAAA
TGCTTTTAAATGGAACCTTGGATATGTCATTAATGGGATCGGTTCAAAATGAACATCACCGAAATTTAGCCTACCAACAC
TTGGGAAAAGACCATTTTACCGCTATCGTTGGTAACCAATCACCTTTCTTTAAACGTGAAAAGATTTGGTTTTCTGAATT
AAAGAATGAAAAATTTCTTACTTTTGATAGTAACTTCGTTCATTCTGAAGCCTTAAAAAATTTTAGTAAGATTACAAACT
TTCGACCTAATATCGTCTATCAAAGTAACGACGCTCAGGTCATTCAAGAAATGGTTGCTCAAAATGTTGGAGTCGGATTT
ATCGCCAATTCAGCACTCACTACCCGCCCAGATATTAAAGCATTGCCTTTTTATGATGAAGACCAACCTTTCTTCTATAT
GAATTTGGTCCAACGTAAAGACCGGATTCTAAGTCCCAATCAACAAAAGATTGCCGATTTAATTTATGGAAATTCGCTAA
TGAAATAA

Upstream 100 bases:

>100_bases
TAATCCCTCGTAAAAACTATAGTCAAATTTATTATTAGTTATAAATATTTTTTATATTAATTCTAAAAATGCTGTTATAA
TAGTATTTCGGAGGTGCAAT

Downstream 100 bases:

>100_bases
AAAAGTTTGATACTTGATTAAAAATCGCGTATCAAACTTTTTTTGAATCTACTTTAGAAACGTGCATATCCTAAAGCATG
TTGCGCAGCTAAATTAATTA

Product: malolactic fermentation system transcription activator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNIKDLNYFHELVKNKNFSSVAEIFHVSQPTISMSVRRLEEEFNTTLFTRDQGRHHLNVTLSGQQLDEHVNAILDHIAIA
QNSINNINTNKLILGLPPIMTKYFFPKIAKQLTEQNLLKAIVPVEKGSAELEKMLLNGTLDMSLMGSVQNEHHRNLAYQH
LGKDHFTAIVGNQSPFFKREKIWFSELKNEKFLTFDSNFVHSEALKNFSKITNFRPNIVYQSNDAQVIQEMVAQNVGVGF
IANSALTTRPDIKALPFYDEDQPFFYMNLVQRKDRILSPNQQKIADLIYGNSLMK

Sequences:

>Translated_295_residues
MNIKDLNYFHELVKNKNFSSVAEIFHVSQPTISMSVRRLEEEFNTTLFTRDQGRHHLNVTLSGQQLDEHVNAILDHIAIA
QNSINNINTNKLILGLPPIMTKYFFPKIAKQLTEQNLLKAIVPVEKGSAELEKMLLNGTLDMSLMGSVQNEHHRNLAYQH
LGKDHFTAIVGNQSPFFKREKIWFSELKNEKFLTFDSNFVHSEALKNFSKITNFRPNIVYQSNDAQVIQEMVAQNVGVGF
IANSALTTRPDIKALPFYDEDQPFFYMNLVQRKDRILSPNQQKIADLIYGNSLMK
>Mature_295_residues
MNIKDLNYFHELVKNKNFSSVAEIFHVSQPTISMSVRRLEEEFNTTLFTRDQGRHHLNVTLSGQQLDEHVNAILDHIAIA
QNSINNINTNKLILGLPPIMTKYFFPKIAKQLTEQNLLKAIVPVEKGSAELEKMLLNGTLDMSLMGSVQNEHHRNLAYQH
LGKDHFTAIVGNQSPFFKREKIWFSELKNEKFLTFDSNFVHSEALKNFSKITNFRPNIVYQSNDAQVIQEMVAQNVGVGF
IANSALTTRPDIKALPFYDEDQPFFYMNLVQRKDRILSPNQQKIADLIYGNSLMK

Specific function: Required for malolactic fermentation. It is most probably a transcriptional activator [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI157672245, Length=270, Percent_Identity=22.962962962963, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787530, Length=268, Percent_Identity=25.3731343283582, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790399, Length=296, Percent_Identity=23.3108108108108, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1788887, Length=245, Percent_Identity=24.0816326530612, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33898; Mature: 33898

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKDLNYFHELVKNKNFSSVAEIFHVSQPTISMSVRRLEEEFNTTLFTRDQGRHHLNVT
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEE
LSGQQLDEHVNAILDHIAIAQNSINNINTNKLILGLPPIMTKYFFPKIAKQLTEQNLLKA
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
IVPVEKGSAELEKMLLNGTLDMSLMGSVQNEHHRNLAYQHLGKDHFTAIVGNQSPFFKRE
HCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
KIWFSELKNEKFLTFDSNFVHSEALKNFSKITNFRPNIVYQSNDAQVIQEMVAQNVGVGF
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEE
IANSALTTRPDIKALPFYDEDQPFFYMNLVQRKDRILSPNQQKIADLIYGNSLMK
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIKDLNYFHELVKNKNFSSVAEIFHVSQPTISMSVRRLEEEFNTTLFTRDQGRHHLNVT
CCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCEEEEECCCCEEEEEE
LSGQQLDEHVNAILDHIAIAQNSINNINTNKLILGLPPIMTKYFFPKIAKQLTEQNLLKA
ECHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
IVPVEKGSAELEKMLLNGTLDMSLMGSVQNEHHRNLAYQHLGKDHFTAIVGNQSPFFKRE
HCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHH
KIWFSELKNEKFLTFDSNFVHSEALKNFSKITNFRPNIVYQSNDAQVIQEMVAQNVGVGF
HHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHCCCCEE
IANSALTTRPDIKALPFYDEDQPFFYMNLVQRKDRILSPNQQKIADLIYGNSLMK
EECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2498286; 11337471 [H]