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The map label for this gene is murF [H]

Identifier: 116493290

GI number: 116493290

Start: 1531083

End: 1532465

Strand: Reverse

Name: murF [H]

Synonym: PEPE_1562

Alternate gene names: 116493290

Gene position: 1532465-1531083 (Counterclockwise)

Preceding gene: 116493291

Following gene: 116493289

Centisome position: 83.63

GC content: 37.74

Gene sequence:

>1383_bases
ATGAAAATGCAACTTAGCGAAATTGCGCGCGCTCTTGGAACGGATGAATATCCTGAACTATGGAACGATATTAGCGTAAC
GAGTGTTCAATTTGATAGTCGAAAATTATCTCCAGGATCACTTTTTGTGCCAATTATGGGTGAACGTGACGGACATGATT
TTATTAGTCAAGCAGTAACAGCAGGCGCCGTTGCAACTTTATGGGCGGAAGACCACCAAGAACGGCGTCCAGCTGATTTA
CCAAGTATTCTAGTAAAAAATCCATTAGAAGCTCTGCAAAAACTTGCTAAATATTATCTGGATAAGATTAACCCTAAGGT
AGTTGCTATTACTGGAAGTAATGGAAAGACAACTACTAAGGATATGACTGCAGCAGTTCTGGAAGCAGAATATAATGTGG
TAAAAACTAAAGCAAACTTTAACAACGAACTGGGCGTGCCAATTACTATTTTGGGAATGAGCTCTAATACGGAAGTATTG
GTAGTTGAAATGGGAATGGATCGTCCAGGACAGTTACACTTTTTAAGTGAATTGGTTCAACCTGATATTGCTGTGATTAC
GATGATTGGCGAAGCCCATATTGAATTTTTTAAGACGCGTGAGCGGATTGCTGATGCAAAAATGGAAATTACAGATGGCC
TAAAAGAGGACGGTTGTTTTGTTTTTAACGGGGATGAACCGCTATTAGAACAGCGTGCTAAAGACCTCAATGTTGAACAA
TACCGTTTTGGGCTTAAAGCAGATAATGATGTCAGCGCCAGCGATATCCAGGGAACTGACACAAGTACAATGTTCAAAAC
TAATTTGGATGAAGAAACCACCGTAACAATTCCAGTATTGGGTGATTACAATGTAACTAATGCGTTAGCAGCAATCGCCG
TTGGACATAAGTACCATATTGACCTAAAAAAAGAAGCTGAAGCGCTAGTTGACTTTGATTTAACCAAAAATCGAACAGAG
TGGTTAACTGGAAAAAAAGGCGAACGTATTTTAAGTGATGTTTATAATTCTAATCCGACTGCTGCTCGAGCAGTATTGGA
TTCATTTGCTAACGTAAATACTGAAGGACGTCGTTTGGTAGTTCTAGGAGATATGTTGGAATTAGGTGAGAATGCTGATC
AAATGCATGCTAGCTTAGCTGAAAAATTAAATGCCAAGCGTTTTGCACAAGTATATTTAATTGGCCCACATATGAAAGCT
CTTGAAAAACGACTGATTGATGAAGATATCTACAGTAAAGATGCTGTTCATTATTATGAAACTGGCGAATTAAAGCGACT
TACAGATGATCTTTCAACTGAAATTCAAGCAAATGATAGTATTTTATTAAAAGCAAGTCACGGAATTCATTTAGAAAATG
TTGTTGATAAAATTAAAAATTAA

Upstream 100 bases:

>100_bases
GGGATAAGATGTTTAGTCTACTTTTAAGGTAAGTTAATTTAATGAGCTATTATGGTATAATTGTAGCCAGGTTTTTTAAT
TTAAGGGGAGTTTTAAAAAG

Downstream 100 bases:

>100_bases
AAAATAAAGTTGTGATGTGGCTTTTAGATTTTTTACAGCGTATGTAAATTATTCTTAAAACGCGGTTGCCGACCTTTGAG
TGGGGAATGCTAGCCTAAAT

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKMQLSEIARALGTDEYPELWNDISVTSVQFDSRKLSPGSLFVPIMGERDGHDFISQAVTAGAVATLWAEDHQERRPADL
PSILVKNPLEALQKLAKYYLDKINPKVVAITGSNGKTTTKDMTAAVLEAEYNVVKTKANFNNELGVPITILGMSSNTEVL
VVEMGMDRPGQLHFLSELVQPDIAVITMIGEAHIEFFKTRERIADAKMEITDGLKEDGCFVFNGDEPLLEQRAKDLNVEQ
YRFGLKADNDVSASDIQGTDTSTMFKTNLDEETTVTIPVLGDYNVTNALAAIAVGHKYHIDLKKEAEALVDFDLTKNRTE
WLTGKKGERILSDVYNSNPTAARAVLDSFANVNTEGRRLVVLGDMLELGENADQMHASLAEKLNAKRFAQVYLIGPHMKA
LEKRLIDEDIYSKDAVHYYETGELKRLTDDLSTEIQANDSILLKASHGIHLENVVDKIKN

Sequences:

>Translated_460_residues
MKMQLSEIARALGTDEYPELWNDISVTSVQFDSRKLSPGSLFVPIMGERDGHDFISQAVTAGAVATLWAEDHQERRPADL
PSILVKNPLEALQKLAKYYLDKINPKVVAITGSNGKTTTKDMTAAVLEAEYNVVKTKANFNNELGVPITILGMSSNTEVL
VVEMGMDRPGQLHFLSELVQPDIAVITMIGEAHIEFFKTRERIADAKMEITDGLKEDGCFVFNGDEPLLEQRAKDLNVEQ
YRFGLKADNDVSASDIQGTDTSTMFKTNLDEETTVTIPVLGDYNVTNALAAIAVGHKYHIDLKKEAEALVDFDLTKNRTE
WLTGKKGERILSDVYNSNPTAARAVLDSFANVNTEGRRLVVLGDMLELGENADQMHASLAEKLNAKRFAQVYLIGPHMKA
LEKRLIDEDIYSKDAVHYYETGELKRLTDDLSTEIQANDSILLKASHGIHLENVVDKIKN
>Mature_460_residues
MKMQLSEIARALGTDEYPELWNDISVTSVQFDSRKLSPGSLFVPIMGERDGHDFISQAVTAGAVATLWAEDHQERRPADL
PSILVKNPLEALQKLAKYYLDKINPKVVAITGSNGKTTTKDMTAAVLEAEYNVVKTKANFNNELGVPITILGMSSNTEVL
VVEMGMDRPGQLHFLSELVQPDIAVITMIGEAHIEFFKTRERIADAKMEITDGLKEDGCFVFNGDEPLLEQRAKDLNVEQ
YRFGLKADNDVSASDIQGTDTSTMFKTNLDEETTVTIPVLGDYNVTNALAAIAVGHKYHIDLKKEAEALVDFDLTKNRTE
WLTGKKGERILSDVYNSNPTAARAVLDSFANVNTEGRRLVVLGDMLELGENADQMHASLAEKLNAKRFAQVYLIGPHMKA
LEKRLIDEDIYSKDAVHYYETGELKRLTDDLSTEIQANDSILLKASHGIHLENVVDKIKN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=444, Percent_Identity=31.981981981982, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51073; Mature: 51073

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMQLSEIARALGTDEYPELWNDISVTSVQFDSRKLSPGSLFVPIMGERDGHDFISQAVT
CCCCHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
AGAVATLWAEDHQERRPADLPSILVKNPLEALQKLAKYYLDKINPKVVAITGSNGKTTTK
HHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
DMTAAVLEAEYNVVKTKANFNNELGVPITILGMSSNTEVLVVEMGMDRPGQLHFLSELVQ
HHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHC
PDIAVITMIGEAHIEFFKTRERIADAKMEITDGLKEDGCFVFNGDEPLLEQRAKDLNVEQ
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCHHH
YRFGLKADNDVSASDIQGTDTSTMFKTNLDEETTVTIPVLGDYNVTNALAAIAVGHKYHI
HEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEE
DLKKEAEALVDFDLTKNRTEWLTGKKGERILSDVYNSNPTAARAVLDSFANVNTEGRRLV
ECHHHHHHHEEECCCCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEE
VLGDMLELGENADQMHASLAEKLNAKRFAQVYLIGPHMKALEKRLIDEDIYSKDAVHYYE
EEECHHHHCCCHHHHHHHHHHHHCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEC
TGELKRLTDDLSTEIQANDSILLKASHGIHLENVVDKIKN
CCCHHHHHHHHHCCEECCCEEEEEECCCCCHHHHHHHHCC
>Mature Secondary Structure
MKMQLSEIARALGTDEYPELWNDISVTSVQFDSRKLSPGSLFVPIMGERDGHDFISQAVT
CCCCHHHHHHHHCCCCCHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHH
AGAVATLWAEDHQERRPADLPSILVKNPLEALQKLAKYYLDKINPKVVAITGSNGKTTTK
HHHHHHHHHHHHHHCCCCCCHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHH
DMTAAVLEAEYNVVKTKANFNNELGVPITILGMSSNTEVLVVEMGMDRPGQLHFLSELVQ
HHHHHHHHHCCEEEEEECCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHC
PDIAVITMIGEAHIEFFKTRERIADAKMEITDGLKEDGCFVFNGDEPLLEQRAKDLNVEQ
CCEEEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHCCCHHH
YRFGLKADNDVSASDIQGTDTSTMFKTNLDEETTVTIPVLGDYNVTNALAAIAVGHKYHI
HEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEE
DLKKEAEALVDFDLTKNRTEWLTGKKGERILSDVYNSNPTAARAVLDSFANVNTEGRRLV
ECHHHHHHHEEECCCCCCHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEE
VLGDMLELGENADQMHASLAEKLNAKRFAQVYLIGPHMKALEKRLIDEDIYSKDAVHYYE
EEECHHHHCCCHHHHHHHHHHHHCHHHEEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEC
TGELKRLTDDLSTEIQANDSILLKASHGIHLENVVDKIKN
CCCHHHHHHHHHCCEECCCEEEEEECCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]