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The map label for this gene is tilS/hprT [H]

Identifier: 116493274

GI number: 116493274

Start: 1514204

End: 1514740

Strand: Reverse

Name: tilS/hprT [H]

Synonym: PEPE_1546

Alternate gene names: 116493274

Gene position: 1514740-1514204 (Counterclockwise)

Preceding gene: 116493275

Following gene: 116493273

Centisome position: 82.66

GC content: 37.43

Gene sequence:

>537_bases
ATGGACAATGATATCGCCAGAGTTTTGTACAGTAAGGCGGATATAAAGGAAGCTTGCGAACGTTTAGGTAAACAATTAAC
AAAGGACTATGCAGGAAAACGTCCTTTAATCTTAAGTGTATTAACGGGTGCTATGTTCTTCACAACGGATGTCGTTCGTG
AATTTGATGTATATTCTGAAATTGATTTTATCGATGTTTCAAGTTACAAAGGTGGCATGGAATCAACGGGAACTGTTGAT
TTGGTGTCAGATTTATCTGAAGATGTTAATGGACGCGATGTTTTAATTATTGAAGATATCGTTGATACTGGGCGGACATT
AAGTTATATTGTGGATTTATTATATTCACGCAATGCGAAAAGCGTTAAGATTTGTACCTTCTTAGACAAGCCAGCTGGAC
GTGTTGTAGAAGCTAAAGCTGACTACGTTGGTTTTACCGTACCTAAAGAGTTTGTAGTGGGTTATGGATTAGACTATCAA
GGCTATTACCGAAACTTACCTTATGTGGGAGTTTTGAAGCCAGAAGTTTACGAATAA

Upstream 100 bases:

>100_bases
ATTTTATGGGTACCAGGTTTGCGAAAGGCCTGGTTAAAGGAACCTTTCCTAAAAGATAATCAACAATTTATTATGGAATT
TAGAATTGGAGGAAGTCCCC

Downstream 100 bases:

>100_bases
AATCATTGGTCAAAGTTAGTCAAATATGGTATTATATCGTCAATGGTCAAGATTGTAACTGATATTTCTTGATGCCTTAC
AAGGAGGAACTACATGAAAA

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 178; Mature: 178

Protein sequence:

>178_residues
MDNDIARVLYSKADIKEACERLGKQLTKDYAGKRPLILSVLTGAMFFTTDVVREFDVYSEIDFIDVSSYKGGMESTGTVD
LVSDLSEDVNGRDVLIIEDIVDTGRTLSYIVDLLYSRNAKSVKICTFLDKPAGRVVEAKADYVGFTVPKEFVVGYGLDYQ
GYYRNLPYVGVLKPEVYE

Sequences:

>Translated_178_residues
MDNDIARVLYSKADIKEACERLGKQLTKDYAGKRPLILSVLTGAMFFTTDVVREFDVYSEIDFIDVSSYKGGMESTGTVD
LVSDLSEDVNGRDVLIIEDIVDTGRTLSYIVDLLYSRNAKSVKICTFLDKPAGRVVEAKADYVGFTVPKEFVVGYGLDYQ
GYYRNLPYVGVLKPEVYE
>Mature_178_residues
MDNDIARVLYSKADIKEACERLGKQLTKDYAGKRPLILSVLTGAMFFTTDVVREFDVYSEIDFIDVSSYKGGMESTGTVD
LVSDLSEDVNGRDVLIIEDIVDTGRTLSYIVDLLYSRNAKSVKICTFLDKPAGRVVEAKADYVGFTVPKEFVVGYGLDYQ
GYYRNLPYVGVLKPEVYE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=180, Percent_Identity=37.2222222222222, Blast_Score=105, Evalue=2e-23,
Organism=Homo sapiens, GI9910262, Length=181, Percent_Identity=33.1491712707182, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI87081689, Length=165, Percent_Identity=45.4545454545455, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17510817, Length=182, Percent_Identity=33.5164835164835, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 19934; Mature: 19934

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNDIARVLYSKADIKEACERLGKQLTKDYAGKRPLILSVLTGAMFFTTDVVREFDVYSE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
IDFIDVSSYKGGMESTGTVDLVSDLSEDVNGRDVLIIEDIVDTGRTLSYIVDLLYSRNAK
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCC
SVKICTFLDKPAGRVVEAKADYVGFTVPKEFVVGYGLDYQGYYRNLPYVGVLKPEVYE
CEEEEEEECCCCCCEEECCCCEECCCCCHHHHEECCCCCCHHHCCCCEEEECCCCCCC
>Mature Secondary Structure
MDNDIARVLYSKADIKEACERLGKQLTKDYAGKRPLILSVLTGAMFFTTDVVREFDVYSE
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
IDFIDVSSYKGGMESTGTVDLVSDLSEDVNGRDVLIIEDIVDTGRTLSYIVDLLYSRNAK
CCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHCCCCC
SVKICTFLDKPAGRVVEAKADYVGFTVPKEFVVGYGLDYQGYYRNLPYVGVLKPEVYE
CEEEEEEECCCCCCEEECCCCEECCCCCHHHHEECCCCCCHHHCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]