Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 116493271

GI number: 116493271

Start: 1510010

End: 1511008

Strand: Reverse

Name: dus1 [H]

Synonym: PEPE_1543

Alternate gene names: 116493271

Gene position: 1511008-1510010 (Counterclockwise)

Preceding gene: 116493272

Following gene: 116493270

Centisome position: 82.46

GC content: 39.64

Gene sequence:

>999_bases
ATGGAATGGAAAATTGGGGACATTACAATCCCAAATCAACTAGTTGTTGCTCCAATGGCCGGTGTGACTAATACTGCTTT
TCGAATGATCTGTAAAAAATTCGGTGCGGGATTAGTGGTCTGCGAAATGATATCCGACCGAGGAATTATCTATGGAAACA
AAAAAACTCTGAGCATGATGACAGTTGATCCAAATGAACATCCAATGAGTATTCAAATTTTTGGTGGTACTAAAGAAACA
TTAGTAGAAGCTGCTAAATTTGTTGATCAAAATACTGATGCAGATATTATTGACATCAATATGGGATGTCCCGTAAACAA
AGTTGTTAATACTGATGCTGGTGCCCGTTGGTTGCTGGATCCTAATAAGGTATATGAGATGGTATCTTACGTGACGGATG
CGGTTAAAAAACCAGTAACAGTTAAAATGCGAACTGGTTGGGATGCTAACCATATTTTAGCGGTTGAAAATGCATTAGCA
GCCGAACGTGCGGGTGCTTCTGCGCTTGCTATGCACGGTCGAACTCGTAAACAAATGTATACTGGACAAGCAGATTGGAA
TATTTTAAAAGAAGTTGGAAGTCAGTTACATATTCCATTTATGGGTAATGGAGATGTACGGACACCTGAAGATGCCAAAA
GAATGTTGGATGAAGTTGGTGCGGACGCGGTAATGATTGGCCGTGCTGTACTGGGTAATCCATGGGAATTACAACGAATT
TCAGCTTATTTAAATGATGGAACAGTTATTGATGAACCTACACCAAGAGAAAAAATTGAGATTGCAAAAGAGCATTTACA
CGCTCTAGTAGAATTGAAGGGTGAAAATGTCGGTCCGCGAGAATTCCGTGGACAAGCTGCCTACTACCTCAAGGGTATTC
CACATTCAGCTCGAACCAAGGTGGCTGTAACGGGAGCAGATACTGAGCAACAAATGATTAATATTTTTGATAGTTTTATT
GAAAAACTCGAAACTAGAAGAGTAAGAACTTTGAATTAA

Upstream 100 bases:

>100_bases
TCATTGACCAAAAATAAGTGAAATTGGTAAGCTTCTCCACAATCATGAATTTCTGTGATATTATATTCTGCATGCATAAA
AAGTTTGACGAGGTGAAAAA

Downstream 100 bases:

>100_bases
GGTTGCAATTTGTTGTTTTAGTAGGTAATAATTATATTTGAATAAATTTAAGAGGGGGACAAAATAGTGGGTGAAAAGCA
ACAACCAAGTATGAATGACC

Product: tRNA-dihydrouridine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEWKIGDITIPNQLVVAPMAGVTNTAFRMICKKFGAGLVVCEMISDRGIIYGNKKTLSMMTVDPNEHPMSIQIFGGTKET
LVEAAKFVDQNTDADIIDINMGCPVNKVVNTDAGARWLLDPNKVYEMVSYVTDAVKKPVTVKMRTGWDANHILAVENALA
AERAGASALAMHGRTRKQMYTGQADWNILKEVGSQLHIPFMGNGDVRTPEDAKRMLDEVGADAVMIGRAVLGNPWELQRI
SAYLNDGTVIDEPTPREKIEIAKEHLHALVELKGENVGPREFRGQAAYYLKGIPHSARTKVAVTGADTEQQMINIFDSFI
EKLETRRVRTLN

Sequences:

>Translated_332_residues
MEWKIGDITIPNQLVVAPMAGVTNTAFRMICKKFGAGLVVCEMISDRGIIYGNKKTLSMMTVDPNEHPMSIQIFGGTKET
LVEAAKFVDQNTDADIIDINMGCPVNKVVNTDAGARWLLDPNKVYEMVSYVTDAVKKPVTVKMRTGWDANHILAVENALA
AERAGASALAMHGRTRKQMYTGQADWNILKEVGSQLHIPFMGNGDVRTPEDAKRMLDEVGADAVMIGRAVLGNPWELQRI
SAYLNDGTVIDEPTPREKIEIAKEHLHALVELKGENVGPREFRGQAAYYLKGIPHSARTKVAVTGADTEQQMINIFDSFI
EKLETRRVRTLN
>Mature_332_residues
MEWKIGDITIPNQLVVAPMAGVTNTAFRMICKKFGAGLVVCEMISDRGIIYGNKKTLSMMTVDPNEHPMSIQIFGGTKET
LVEAAKFVDQNTDADIIDINMGCPVNKVVNTDAGARWLLDPNKVYEMVSYVTDAVKKPVTVKMRTGWDANHILAVENALA
AERAGASALAMHGRTRKQMYTGQADWNILKEVGSQLHIPFMGNGDVRTPEDAKRMLDEVGADAVMIGRAVLGNPWELQRI
SAYLNDGTVIDEPTPREKIEIAKEHLHALVELKGENVGPREFRGQAAYYLKGIPHSARTKVAVTGADTEQQMINIFDSFI
EKLETRRVRTLN

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=235, Percent_Identity=30.2127659574468, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI239788462, Length=229, Percent_Identity=30.5676855895196, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI40807366, Length=269, Percent_Identity=28.2527881040892, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI8923374, Length=255, Percent_Identity=26.6666666666667, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI31742496, Length=223, Percent_Identity=29.5964125560538, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1789660, Length=322, Percent_Identity=34.1614906832298, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1788462, Length=242, Percent_Identity=30.5785123966942, Blast_Score=102, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17543114, Length=231, Percent_Identity=30.7359307359307, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17507177, Length=247, Percent_Identity=31.5789473684211, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=219, Percent_Identity=26.9406392694064, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17510279, Length=247, Percent_Identity=26.7206477732794, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323560, Length=230, Percent_Identity=30, Blast_Score=102, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6323433, Length=283, Percent_Identity=26.8551236749117, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=223, Percent_Identity=23.7668161434978, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585320, Length=237, Percent_Identity=32.0675105485232, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24580595, Length=224, Percent_Identity=32.1428571428571, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI19920448, Length=224, Percent_Identity=32.1428571428571, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI45549423, Length=235, Percent_Identity=27.6595744680851, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=28.125, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 36711; Mature: 36711

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEWKIGDITIPNQLVVAPMAGVTNTAFRMICKKFGAGLVVCEMISDRGIIYGNKKTLSMM
CCEEECCEECCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHCCCEEEECCCEEEEE
TVDPNEHPMSIQIFGGTKETLVEAAKFVDQNTDADIIDINMGCPVNKVVNTDAGARWLLD
EECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEC
PNKVYEMVSYVTDAVKKPVTVKMRTGWDANHILAVENALAAERAGASALAMHGRTRKQMY
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCHHHHHCCCHHHHHC
TGQADWNILKEVGSQLHIPFMGNGDVRTPEDAKRMLDEVGADAVMIGRAVLGNPWELQRI
CCCCHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECHHHHCCCHHHHHH
SAYLNDGTVIDEPTPREKIEIAKEHLHALVELKGENVGPREFRGQAAYYLKGIPHSARTK
HHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCCCCE
VAVTGADTEQQMINIFDSFIEKLETRRVRTLN
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MEWKIGDITIPNQLVVAPMAGVTNTAFRMICKKFGAGLVVCEMISDRGIIYGNKKTLSMM
CCEEECCEECCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEHHCCCEEEECCCEEEEE
TVDPNEHPMSIQIFGGTKETLVEAAKFVDQNTDADIIDINMGCPVNKVVNTDAGARWLLD
EECCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHCCCCCCCEEEEC
PNKVYEMVSYVTDAVKKPVTVKMRTGWDANHILAVENALAAERAGASALAMHGRTRKQMY
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHHHHHHHHCCCHHHHHCCCHHHHHC
TGQADWNILKEVGSQLHIPFMGNGDVRTPEDAKRMLDEVGADAVMIGRAVLGNPWELQRI
CCCCHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECHHHHCCCHHHHHH
SAYLNDGTVIDEPTPREKIEIAKEHLHALVELKGENVGPREFRGQAAYYLKGIPHSARTK
HHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEEEECCCCCCCCE
VAVTGADTEQQMINIFDSFIEKLETRRVRTLN
EEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]