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The map label for this gene is lysS

Identifier: 116493270

GI number: 116493270

Start: 1508447

End: 1509943

Strand: Reverse

Name: lysS

Synonym: PEPE_1542

Alternate gene names: 116493270

Gene position: 1509943-1508447 (Counterclockwise)

Preceding gene: 116493271

Following gene: 116493269

Centisome position: 82.4

GC content: 40.41

Gene sequence:

>1497_bases
GTGGGTGAAAAGCAACAACCAAGTATGAATGACCAACTAAGAGTTCGTCGCGAAAAGATGCAAGAACTTCGTGATAAGGG
CATCGATCCATTTGGTCATCGTTTTGAGAGAACACATTTAGCAGCACAATTACACGAAAAGTACAGTGATATGACAAAGG
AAGAGCTTGATGAACAGGGCGTGGTAGCTACGATTGCCGGCCGTATGATCTCTAAGCGTGGTAAGGGTAAGGTCGGTTTT
GCAGATCTTAAAGACCGTTCTGGAAAAATGCAATTGTACGTTCGTAAGGATGTTTTAGGCGAAGATGTATACAAAGTCTT
TAAGCGTTCTGATATTGGTGATTTCCTCGGTGTAACTGGGGATGTCATGAAAACCGATATGGGTGAATTAACAATTAAAG
TAACGGGACTAACTTTCTTAAGTAAAGCGCTCCGTCCTCTTCCCGACAAGTTCCATGGTTTACAAAACGTTGAACAAATT
TATCGTCAACGTTATCTTGATTTAATTGCTAATGATGATAGTATGGATCGTTTCAGAAAACGGACTAAGATTGTTTCAGC
AGTAAGAAGATATCTTGATGGTAATGATTTTGCCGAAGTTGAAACACCAGTGCTACATACTCAAGCTGGTGGAGCTAGTG
CACGTCCATTTATTACACACCATAATGCCCTAGATATCAATTTATATTTACGGATTGCGTTGGAACTTCATTTAAAACGG
CTAATTGTTGGTGGAATGGAACGAGTATACGAAATCGGTCGCGTATTCAGAAATGAAGGGATTGATACTCGTCATAATCC
TGAATTCACTATGCTCGAAACATACGCAGCTTACTTTGATTTCCATGACGTAATGGATGAAACTGAAGGAATTTTTAAGG
CAGCAGTTGCAGCGGTTTCAGATGATGGTAAGATTACGTACCAAGGAACAGAACTTGACTTAGGTCAAGATTTTGAACGT
CTACACATGGTCGATGCAATCAAGAAATATGCGGGAGTGGACTTTTGGCCTAAGATGTCGGTTGAAGATGCGAAGAAACT
TGCTGATGAAAATGGTATCCACTACGAGTCTTGGTGGACAGTTGGACACATCATCAATGAATTCTTTGAAGAAAAGGTTC
AAGACCAATTGAAACAACCGGTGTTTATTTACGGACATCCAGTAGAAATTTCTCCACTAGCTAAGAAGAACGCCGAAGAT
GATCGTTTTACAGATCGATTTGAGTTGTACATCTTAGGTAATGAATATGGTAATGCCTTTACTGAATTAAATGATCCTAT
CGACCAACGGGCACGTTTTGAAGCTCAAGTTGCAGAACGTGAAGCAGGTAACGATGAAGCTGAAGGTATTGACGAAGATT
ATATCGAAGCACTTGAATATGGTATGCCACCAACAGGGGGACTTGGAATTGGGATTGATCGTTTAGTGATGTTGCTAACT
GATGCTCCTTCAATTCGTGATGTACTATTGTTCCCAACGATGAAACCCATCGATTAA

Upstream 100 bases:

>100_bases
ACTCGAAACTAGAAGAGTAAGAACTTTGAATTAAGGTTGCAATTTGTTGTTTTAGTAGGTAATAATTATATTTGAATAAA
TTTAAGAGGGGGACAAAATA

Downstream 100 bases:

>100_bases
ATAAGAACTAAGAAAAGAATGAGAATAGCAAAATTCTCATTCTTTTTTTGTGCATTTTACGCAAGGAAAAAGATGTTTTT
TTAGCTTCATGAAATCGTTA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 498; Mature: 497

Protein sequence:

>498_residues
MGEKQQPSMNDQLRVRREKMQELRDKGIDPFGHRFERTHLAAQLHEKYSDMTKEELDEQGVVATIAGRMISKRGKGKVGF
ADLKDRSGKMQLYVRKDVLGEDVYKVFKRSDIGDFLGVTGDVMKTDMGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQI
YRQRYLDLIANDDSMDRFRKRTKIVSAVRRYLDGNDFAEVETPVLHTQAGGASARPFITHHNALDINLYLRIALELHLKR
LIVGGMERVYEIGRVFRNEGIDTRHNPEFTMLETYAAYFDFHDVMDETEGIFKAAVAAVSDDGKITYQGTELDLGQDFER
LHMVDAIKKYAGVDFWPKMSVEDAKKLADENGIHYESWWTVGHIINEFFEEKVQDQLKQPVFIYGHPVEISPLAKKNAED
DRFTDRFELYILGNEYGNAFTELNDPIDQRARFEAQVAEREAGNDEAEGIDEDYIEALEYGMPPTGGLGIGIDRLVMLLT
DAPSIRDVLLFPTMKPID

Sequences:

>Translated_498_residues
MGEKQQPSMNDQLRVRREKMQELRDKGIDPFGHRFERTHLAAQLHEKYSDMTKEELDEQGVVATIAGRMISKRGKGKVGF
ADLKDRSGKMQLYVRKDVLGEDVYKVFKRSDIGDFLGVTGDVMKTDMGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQI
YRQRYLDLIANDDSMDRFRKRTKIVSAVRRYLDGNDFAEVETPVLHTQAGGASARPFITHHNALDINLYLRIALELHLKR
LIVGGMERVYEIGRVFRNEGIDTRHNPEFTMLETYAAYFDFHDVMDETEGIFKAAVAAVSDDGKITYQGTELDLGQDFER
LHMVDAIKKYAGVDFWPKMSVEDAKKLADENGIHYESWWTVGHIINEFFEEKVQDQLKQPVFIYGHPVEISPLAKKNAED
DRFTDRFELYILGNEYGNAFTELNDPIDQRARFEAQVAEREAGNDEAEGIDEDYIEALEYGMPPTGGLGIGIDRLVMLLT
DAPSIRDVLLFPTMKPID
>Mature_497_residues
GEKQQPSMNDQLRVRREKMQELRDKGIDPFGHRFERTHLAAQLHEKYSDMTKEELDEQGVVATIAGRMISKRGKGKVGFA
DLKDRSGKMQLYVRKDVLGEDVYKVFKRSDIGDFLGVTGDVMKTDMGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIY
RQRYLDLIANDDSMDRFRKRTKIVSAVRRYLDGNDFAEVETPVLHTQAGGASARPFITHHNALDINLYLRIALELHLKRL
IVGGMERVYEIGRVFRNEGIDTRHNPEFTMLETYAAYFDFHDVMDETEGIFKAAVAAVSDDGKITYQGTELDLGQDFERL
HMVDAIKKYAGVDFWPKMSVEDAKKLADENGIHYESWWTVGHIINEFFEEKVQDQLKQPVFIYGHPVEISPLAKKNAEDD
RFTDRFELYILGNEYGNAFTELNDPIDQRARFEAQVAEREAGNDEAEGIDEDYIEALEYGMPPTGGLGIGIDRLVMLLTD
APSIRDVLLFPTMKPID

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=40.4669260700389, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=40.8284023668639, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI40789249, Length=246, Percent_Identity=25.609756097561, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1789256, Length=491, Percent_Identity=51.5274949083503, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI1790571, Length=497, Percent_Identity=51.7102615694165, Blast_Score=509, Evalue=1e-145,
Organism=Escherichia coli, GI87082379, Length=326, Percent_Identity=31.2883435582822, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788173, Length=248, Percent_Identity=27.4193548387097, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787161, Length=348, Percent_Identity=24.1379310344828, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=504, Percent_Identity=41.6666666666667, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17535927, Length=504, Percent_Identity=41.6666666666667, Blast_Score=379, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71994340, Length=503, Percent_Identity=41.7495029821074, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI17551876, Length=323, Percent_Identity=24.7678018575851, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=509, Percent_Identity=41.8467583497053, Blast_Score=371, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=536, Percent_Identity=32.8358208955224, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI24640849, Length=517, Percent_Identity=40.2321083172147, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=517, Percent_Identity=40.2321083172147, Blast_Score=375, Evalue=1e-104,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_PEDPA (Q03E09)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_805005.1
- ProteinModelPortal:   Q03E09
- SMR:   Q03E09
- STRING:   Q03E09
- GeneID:   4417174
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1542
- NMPDR:   fig|278197.10.peg.1351
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   RNEGMDH
- PhylomeDB:   Q03E09
- ProtClustDB:   PRK00484
- BioCyc:   PPEN278197:PEPE_1542-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56919; Mature: 56788

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKQQPSMNDQLRVRREKMQELRDKGIDPFGHRFERTHLAAQLHEKYSDMTKEELDEQG
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVATIAGRMISKRGKGKVGFADLKDRSGKMQLYVRKDVLGEDVYKVFKRSDIGDFLGVTG
CHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCCCC
DVMKTDMGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIYRQRYLDLIANDDSMDRFRK
HHHHCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTKIVSAVRRYLDGNDFAEVETPVLHTQAGGASARPFITHHNALDINLYLRIALELHLKR
HHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCEEEEEHHHHHHHHHHHHH
LIVGGMERVYEIGRVFRNEGIDTRHNPEFTMLETYAAYFDFHDVMDETEGIFKAAVAAVS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDGKITYQGTELDLGQDFERLHMVDAIKKYAGVDFWPKMSVEDAKKLADENGIHYESWWT
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEHHHHH
VGHIINEFFEEKVQDQLKQPVFIYGHPVEISPLAKKNAEDDRFTDRFELYILGNEYGNAF
HHHHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHCCCCCCCCCCEEEEEEEECCCCCHH
TELNDPIDQRARFEAQVAEREAGNDEAEGIDEDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DAPSIRDVLLFPTMKPID
CCCCHHHEEECCCCCCCC
>Mature Secondary Structure 
GEKQQPSMNDQLRVRREKMQELRDKGIDPFGHRFERTHLAAQLHEKYSDMTKEELDEQG
CCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VVATIAGRMISKRGKGKVGFADLKDRSGKMQLYVRKDVLGEDVYKVFKRSDIGDFLGVTG
CHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCHHHHCCCC
DVMKTDMGELTIKVTGLTFLSKALRPLPDKFHGLQNVEQIYRQRYLDLIANDDSMDRFRK
HHHHCCCCCEEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
RTKIVSAVRRYLDGNDFAEVETPVLHTQAGGASARPFITHHNALDINLYLRIALELHLKR
HHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEEEECCEEEEEHHHHHHHHHHHHH
LIVGGMERVYEIGRVFRNEGIDTRHNPEFTMLETYAAYFDFHDVMDETEGIFKAAVAAVS
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DDGKITYQGTELDLGQDFERLHMVDAIKKYAGVDFWPKMSVEDAKKLADENGIHYESWWT
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCEEHHHHH
VGHIINEFFEEKVQDQLKQPVFIYGHPVEISPLAKKNAEDDRFTDRFELYILGNEYGNAF
HHHHHHHHHHHHHHHHHCCCEEEECCCEEECCHHHCCCCCCCCCCEEEEEEEECCCCCHH
TELNDPIDQRARFEAQVAEREAGNDEAEGIDEDYIEALEYGMPPTGGLGIGIDRLVMLLT
HHCCCCHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
DAPSIRDVLLFPTMKPID
CCCCHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA