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The map label for this gene is nadE [H]

Identifier: 116493261

GI number: 116493261

Start: 1493317

End: 1494135

Strand: Reverse

Name: nadE [H]

Synonym: PEPE_1524

Alternate gene names: 116493261

Gene position: 1494135-1493317 (Counterclockwise)

Preceding gene: 116493262

Following gene: 116493260

Centisome position: 81.54

GC content: 42.37

Gene sequence:

>819_bases
TTGCGAGAATTACAAAAAGAGATTATTGAAACTTTAAAGGTTCAACCGACGATTGACCCAAAACAGGAAATTCGTCGTAG
TGTAGATTTTATGAAAAATTATCTAAAAAAAGTTCCTAGTTTACGTTCCTTAGTGCTCGGAATCTCAGGTGGACAGGATT
CAACCCTTGCCGGTGCGTTAGCTGAAATTGCGGTTCGTGAATTACGGGAAGAAACGGGTATTCAAGACTACCGGTTCATT
GCAGTTCGCTTGCCCTATGGTGTGCAAGCGGATGAATCCGATGCAATGAAAGCAATCGAATTTATGCAAGCCGATGAAGT
AGTTCGAATTGATATCAAACCAGCGGCAGATGCCATGGTTGCTGCGTTGGAAGCTAATGATTTAAAAATTTCTGATTTTA
ACAAAGGGAATATCAAAGCTCGTGAACGGATGATTGCTCAATATGGTATTGCGGGAAGTAATGCTGGAATTGTAATTGGA
ACCGATCATGCTGCAGAATCTGTTACGGGATTTTATACTAAATTTGGTGATGGCGGAGCTGATATTATCCCTCTTTGGCG
CTTGGATAAGCGACAAGGAAAGGCAATGCTAAAAGAGCTCAACGCACCGGTTGAACTTTATGAAAAGACCCCAACGGCTG
ATTTAGAAGAGGAGCGTCCAGCGTTGCCTGACGAAGTGGCCTTAGGGGTTACTTACGACGATATTGACAACTATCTGGAA
GGTCGCGAAATTAGTTCTGAAGCGGCTGACAAAATTGAAGGTTGGTATAAACGGACGGCTCATAAACGAGTTTTACCTTA
TACGGTTTTCGACAACTAA

Upstream 100 bases:

>100_bases
TGGATCTTTCACAAGATTGCTACGACAATAAAGTTGCTATTATCAAGCGAGTCCGTGAATATGTTGGGAAACTAAAATTT
TAACAAACTAGGTGATAAAA

Downstream 100 bases:

>100_bases
TTATTAAGAGAAGGGGTTTAGCTTGTAATTAGTTTGAATGGTCGGTTATAATCAAATTGTAGACATGAGGGAGTAACTTG
CAGACTATCTGCGATAATAT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MRELQKEIIETLKVQPTIDPKQEIRRSVDFMKNYLKKVPSLRSLVLGISGGQDSTLAGALAEIAVRELREETGIQDYRFI
AVRLPYGVQADESDAMKAIEFMQADEVVRIDIKPAADAMVAALEANDLKISDFNKGNIKARERMIAQYGIAGSNAGIVIG
TDHAAESVTGFYTKFGDGGADIIPLWRLDKRQGKAMLKELNAPVELYEKTPTADLEEERPALPDEVALGVTYDDIDNYLE
GREISSEAADKIEGWYKRTAHKRVLPYTVFDN

Sequences:

>Translated_272_residues
MRELQKEIIETLKVQPTIDPKQEIRRSVDFMKNYLKKVPSLRSLVLGISGGQDSTLAGALAEIAVRELREETGIQDYRFI
AVRLPYGVQADESDAMKAIEFMQADEVVRIDIKPAADAMVAALEANDLKISDFNKGNIKARERMIAQYGIAGSNAGIVIG
TDHAAESVTGFYTKFGDGGADIIPLWRLDKRQGKAMLKELNAPVELYEKTPTADLEEERPALPDEVALGVTYDDIDNYLE
GREISSEAADKIEGWYKRTAHKRVLPYTVFDN
>Mature_272_residues
MRELQKEIIETLKVQPTIDPKQEIRRSVDFMKNYLKKVPSLRSLVLGISGGQDSTLAGALAEIAVRELREETGIQDYRFI
AVRLPYGVQADESDAMKAIEFMQADEVVRIDIKPAADAMVAALEANDLKISDFNKGNIKARERMIAQYGIAGSNAGIVIG
TDHAAESVTGFYTKFGDGGADIIPLWRLDKRQGKAMLKELNAPVELYEKTPTADLEEERPALPDEVALGVTYDDIDNYLE
GREISSEAADKIEGWYKRTAHKRVLPYTVFDN

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=269, Percent_Identity=60.2230483271376, Blast_Score=339, Evalue=1e-94,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30282; Mature: 30282

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRELQKEIIETLKVQPTIDPKQEIRRSVDFMKNYLKKVPSLRSLVLGISGGQDSTLAGAL
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHH
AEIAVRELREETGIQDYRFIAVRLPYGVQADESDAMKAIEFMQADEVVRIDIKPAADAMV
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHH
AALEANDLKISDFNKGNIKARERMIAQYGIAGSNAGIVIGTDHAAESVTGFYTKFGDGGA
HHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
DIIPLWRLDKRQGKAMLKELNAPVELYEKTPTADLEEERPALPDEVALGVTYDDIDNYLE
CEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHC
GREISSEAADKIEGWYKRTAHKRVLPYTVFDN
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCC
>Mature Secondary Structure
MRELQKEIIETLKVQPTIDPKQEIRRSVDFMKNYLKKVPSLRSLVLGISGGQDSTLAGAL
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHEECCCCCCHHHHHHH
AEIAVRELREETGIQDYRFIAVRLPYGVQADESDAMKAIEFMQADEVVRIDIKPAADAMV
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHH
AALEANDLKISDFNKGNIKARERMIAQYGIAGSNAGIVIGTDHAAESVTGFYTKFGDGGA
HHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCC
DIIPLWRLDKRQGKAMLKELNAPVELYEKTPTADLEEERPALPDEVALGVTYDDIDNYLE
CEEEEECCCHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHC
GREISSEAADKIEGWYKRTAHKRVLPYTVFDN
CCHHHHHHHHHHHHHHHHHHCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA