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The map label for this gene is radA [H]

Identifier: 116493241

GI number: 116493241

Start: 1470729

End: 1472099

Strand: Reverse

Name: radA [H]

Synonym: PEPE_1503

Alternate gene names: 116493241

Gene position: 1472099-1470729 (Counterclockwise)

Preceding gene: 116493242

Following gene: 116493240

Centisome position: 80.34

GC content: 41.21

Gene sequence:

>1371_bases
TTGGCTAAAGTAAAAACACAGTTTTTGTGTTCAGAATGTGGGTATGTTTCACCTAAATTCTTAGGACGTTGTCCTAATTG
TGGAAGATGGAACACTCTGGTTGAAGAGCGGATTAGTGAACCTAAAAATGAACGTAAGAGTCGCGTTAGTTTTGAGGGTA
AAAGTAGTAAACCACAACTAATTTCAGAAGTTAAAATGCACGAAGAACCGCGGGTTAAAACAGGGATGGAAGAACTGAAC
CGAGTGCTCGGTGGTGGAGTAGTGGATGGCTCACTAGTTCTAATTGGTGGAGATCCAGGAATTGGAAAGTCGACCCTGTT
GTTACAACTATCTGGGCAATTAGCTGCTACTGATCGAGATGTTTTATACGTGTCTGGGGAAGAAAGTGCTTCACAGATCA
AGATGCGCGCCGAACGTTTACAGGTTAACAGTGAACGTTTTTACTTGTACCCTGAAACTGATATGTCCAATATTCGTGCG
GTCATTGAGGAACTACATCCTCAGTATGTAATTATTGATTCTGTTCAAACCATGCAAGAGCCCGACATTGATTCAGCAGT
AGGTAGTGTATCGCAAATTCGAGAAATTACAGCCGAATTAATGCAGATTGCTAAAACAAATAACATTACTATTTTTATTG
TTGGTCATGTAACTAAAGGCGGAGCCATTGCTGGTCCTAAAATTTTAGAACATATGGTTGATACAGTACTTTATTTTGAA
GGCGATTTGCATCATACGTATCGAATTTTACGAGCGGTTAAAAATCGATTTGGTTCTACGAATGAATTGGGAATTTTCGA
AATGCGAGAGGAAGGGCTTCGAGAAGTAGCCAATCCTTCGGAAATCTTTTTAGAGGAACGCCTGCAAGATGCTAACGGTT
CCGCTATTGTGGTTTCGATGGAAGGTACTCGACCAATTTTGGTCGAAATTCAAGCTTTGATTACGCCTACCATTTTTGGG
AATGCTCAGCGGACTGCTAGCGGGTTGGATCGTAACCGAGTCTCATTGATTATGGCAGTTTTAGAAAAAAGGGCTAATTT
AACGTTGCAGAACCAAGACGCCTACTTAAAGGCTGCTGGCGGAGTGAAATTGGATGAGCCAGCAATTGATTTGGCAATTG
CGATGAGTATTGTATCGAGTTTTAAGAATCAAGGGACGAGACCGACTGATAGCTTTGTTGGCGAAATTGGTTTAACTGGT
GAAATCCGACGGGTTAATCGGATTGAACAACGGGTTGCCGAAGCTCAAAAACTAGGTTTTCAACGAATTTTAATTCCTAA
GAATAATTTGAAGGGTTGGAATCCACCTAAAGGGATTGAAGTAGTAGGAGTAACAACGATTACGGACGCAATTCGGAAGG
CGTTTAATTAG

Upstream 100 bases:

>100_bases
GTCATTAAAAATCAATAAAGGTCAAACTAATTTTGATTAAATTATAGAAATAAATGCTATCCTAATTGTACGAATTTAAA
GTTGAGGGGGAATTTAAAGC

Downstream 100 bases:

>100_bases
AAAGGAGGTGAATTAAAATGAAAAATATTAAAAAACGAATTGTCCAAGCGGTGTTTGCTTTAGCTGGTGGTACTTTAGGA
ATGTCGTATCTACCAGTTGT

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MAKVKTQFLCSECGYVSPKFLGRCPNCGRWNTLVEERISEPKNERKSRVSFEGKSSKPQLISEVKMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAATDRDVLYVSGEESASQIKMRAERLQVNSERFYLYPETDMSNIRA
VIEELHPQYVIIDSVQTMQEPDIDSAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLQDANGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRANLTLQNQDAYLKAAGGVKLDEPAIDLAIAMSIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFQRILIPKNNLKGWNPPKGIEVVGVTTITDAIRKAFN

Sequences:

>Translated_456_residues
MAKVKTQFLCSECGYVSPKFLGRCPNCGRWNTLVEERISEPKNERKSRVSFEGKSSKPQLISEVKMHEEPRVKTGMEELN
RVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAATDRDVLYVSGEESASQIKMRAERLQVNSERFYLYPETDMSNIRA
VIEELHPQYVIIDSVQTMQEPDIDSAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLQDANGSAIVVSMEGTRPILVEIQALITPTIFG
NAQRTASGLDRNRVSLIMAVLEKRANLTLQNQDAYLKAAGGVKLDEPAIDLAIAMSIVSSFKNQGTRPTDSFVGEIGLTG
EIRRVNRIEQRVAEAQKLGFQRILIPKNNLKGWNPPKGIEVVGVTTITDAIRKAFN
>Mature_455_residues
AKVKTQFLCSECGYVSPKFLGRCPNCGRWNTLVEERISEPKNERKSRVSFEGKSSKPQLISEVKMHEEPRVKTGMEELNR
VLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAATDRDVLYVSGEESASQIKMRAERLQVNSERFYLYPETDMSNIRAV
IEELHPQYVIIDSVQTMQEPDIDSAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFEG
DLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLQDANGSAIVVSMEGTRPILVEIQALITPTIFGN
AQRTASGLDRNRVSLIMAVLEKRANLTLQNQDAYLKAAGGVKLDEPAIDLAIAMSIVSSFKNQGTRPTDSFVGEIGLTGE
IRRVNRIEQRVAEAQKLGFQRILIPKNNLKGWNPPKGIEVVGVTTITDAIRKAFN

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=457, Percent_Identity=44.6389496717724, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 50362; Mature: 50230

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKVKTQFLCSECGYVSPKFLGRCPNCGRWNTLVEERISEPKNERKSRVSFEGKSSKPQL
CCCCHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHH
ISEVKMHEEPRVKTGMEELNRVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAATDRD
HHHHHHCCCCCHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEECCCEEECCCC
VLYVSGEESASQIKMRAERLQVNSERFYLYPETDMSNIRAVIEELHPQYVIIDSVQTMQE
EEEEECCCHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDIDSAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLQDANGSAIVVSM
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEE
EGTRPILVEIQALITPTIFGNAQRTASGLDRNRVSLIMAVLEKRANLTLQNQDAYLKAAG
CCCCCEEEEHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCEEEECC
GVKLDEPAIDLAIAMSIVSSFKNQGTRPTDSFVGEIGLTGEIRRVNRIEQRVAEAQKLGF
CCEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
QRILIPKNNLKGWNPPKGIEVVGVTTITDAIRKAFN
EEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHCC
>Mature Secondary Structure 
AKVKTQFLCSECGYVSPKFLGRCPNCGRWNTLVEERISEPKNERKSRVSFEGKSSKPQL
CCCHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHH
ISEVKMHEEPRVKTGMEELNRVLGGGVVDGSLVLIGGDPGIGKSTLLLQLSGQLAATDRD
HHHHHHCCCCCHHHHHHHHHHHHCCCEECCEEEEEECCCCCCCCEEEEEECCCEEECCCC
VLYVSGEESASQIKMRAERLQVNSERFYLYPETDMSNIRAVIEELHPQYVIIDSVQTMQE
EEEEECCCHHHHHHHHHHHHEECCCEEEEECCCCHHHHHHHHHHHCCCEEEEECHHHHCC
PDIDSAVGSVSQIREITAELMQIAKTNNITIFIVGHVTKGGAIAGPKILEHMVDTVLYFE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCC
GDLHHTYRILRAVKNRFGSTNELGIFEMREEGLREVANPSEIFLEERLQDANGSAIVVSM
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEE
EGTRPILVEIQALITPTIFGNAQRTASGLDRNRVSLIMAVLEKRANLTLQNQDAYLKAAG
CCCCCEEEEHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCEEEECC
GVKLDEPAIDLAIAMSIVSSFKNQGTRPTDSFVGEIGLTGEIRRVNRIEQRVAEAQKLGF
CCEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCC
QRILIPKNNLKGWNPPKGIEVVGVTTITDAIRKAFN
EEEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8016066 [H]