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The map label for this gene is purN [H]

Identifier: 116493197

GI number: 116493197

Start: 1433158

End: 1433739

Strand: Reverse

Name: purN [H]

Synonym: PEPE_1459

Alternate gene names: 116493197

Gene position: 1433739-1433158 (Counterclockwise)

Preceding gene: 116493198

Following gene: 116493196

Centisome position: 78.24

GC content: 38.14

Gene sequence:

>582_bases
ATGATTAATATTGCAGTTTTTGCATCAGGAACAGGGACTAATTTTATGGCATTATACCAACATATTCGAGAAACAAAAGT
TCCCATTAAAATTGCATGCTTAATTTGTGACCAACCCAATGCGCCGGTTGTTACTAAAGCTGATGAGTTAGGAATTCCAG
TCTGGACCCACCGTTTGAGAGAATTTGAAGATAAAGTGAGCTATGAAAAGGCGATTTTACGTGAGCTAAAAAAATATAAT
TTAGCATTAATCATTTTAGCTGGGTACATGAAGATTGTTACCAAAGTTTTGTTAGAGGCTTATCCACATGCCATTCTTAA
TATCCATCCAGCGCTGTTACCATCGTTTCCGGGGCGCCATGGAATTGAGGATGCGTTTGAATATGGAGTAAAAATTACGG
GAGTAACGATTCACTGGATTGACGGAGGGATTGATACTGGCCCGATTATTGCTCAGCAACCTGTACCGGTTTTACAAGGG
GATGATGTAGAGCATTTAGCTCAACGTATCCATCAAGTTGAACATGATTTATATTTTAGAAGCATCTGCCAAGTATTAAA
ACAGAGAAAGTTGATTAACTAA

Upstream 100 bases:

>100_bases
TAGCAGAACTTGAACAACAGAAAGAAACATATTTTAAGATTGGTAAAGTAAAAAAACGGGAGAAAGATGCACCAGCGGTT
TACCTTTCAGGAGGGAGCAG

Downstream 100 bases:

>100_bases
GGAGAGATTAAGCCATGAAAACTGCATTACTGAGTGTGTCAGATAAAACTGGAATCGTTGAATTTGCTAGAGGTTTAGTT
AAGGCAGATTTTAGAATCAT

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MINIAVFASGTGTNFMALYQHIRETKVPIKIACLICDQPNAPVVTKADELGIPVWTHRLREFEDKVSYEKAILRELKKYN
LALIILAGYMKIVTKVLLEAYPHAILNIHPALLPSFPGRHGIEDAFEYGVKITGVTIHWIDGGIDTGPIIAQQPVPVLQG
DDVEHLAQRIHQVEHDLYFRSICQVLKQRKLIN

Sequences:

>Translated_193_residues
MINIAVFASGTGTNFMALYQHIRETKVPIKIACLICDQPNAPVVTKADELGIPVWTHRLREFEDKVSYEKAILRELKKYN
LALIILAGYMKIVTKVLLEAYPHAILNIHPALLPSFPGRHGIEDAFEYGVKITGVTIHWIDGGIDTGPIIAQQPVPVLQG
DDVEHLAQRIHQVEHDLYFRSICQVLKQRKLIN
>Mature_193_residues
MINIAVFASGTGTNFMALYQHIRETKVPIKIACLICDQPNAPVVTKADELGIPVWTHRLREFEDKVSYEKAILRELKKYN
LALIILAGYMKIVTKVLLEAYPHAILNIHPALLPSFPGRHGIEDAFEYGVKITGVTIHWIDGGIDTGPIIAQQPVPVLQG
DDVEHLAQRIHQVEHDLYFRSICQVLKQRKLIN

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=36.7231638418079, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=36.7231638418079, Blast_Score=128, Evalue=4e-30,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=36.7231638418079, Blast_Score=128, Evalue=4e-30,
Organism=Escherichia coli, GI1788846, Length=177, Percent_Identity=30.5084745762712, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787483, Length=128, Percent_Identity=30.46875, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=177, Percent_Identity=34.4632768361582, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24582400, Length=192, Percent_Identity=34.8958333333333, Blast_Score=130, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21752; Mature: 21752

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIAVFASGTGTNFMALYQHIRETKVPIKIACLICDQPNAPVVTKADELGIPVWTHRLR
CEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHCCHHHHHHHH
EFEDKVSYEKAILRELKKYNLALIILAGYMKIVTKVLLEAYPHAILNIHPALLPSFPGRH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCC
GIEDAFEYGVKITGVTIHWIDGGIDTGPIIAQQPVPVLQGDDVEHLAQRIHQVEHDLYFR
CCHHHHHCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SICQVLKQRKLIN
HHHHHHHHHCCCC
>Mature Secondary Structure
MINIAVFASGTGTNFMALYQHIRETKVPIKIACLICDQPNAPVVTKADELGIPVWTHRLR
CEEEEEEECCCCCHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEECHHHHCCHHHHHHHH
EFEDKVSYEKAILRELKKYNLALIILAGYMKIVTKVLLEAYPHAILNIHPALLPSFPGRH
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCC
GIEDAFEYGVKITGVTIHWIDGGIDTGPIIAQQPVPVLQGDDVEHLAQRIHQVEHDLYFR
CCHHHHHCCEEEEEEEEEEECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SICQVLKQRKLIN
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]