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The map label for this gene is purH

Identifier: 116493196

GI number: 116493196

Start: 1431608

End: 1433143

Strand: Reverse

Name: purH

Synonym: PEPE_1458

Alternate gene names: 116493196

Gene position: 1433143-1431608 (Counterclockwise)

Preceding gene: 116493197

Following gene: 116493195

Centisome position: 78.21

GC content: 38.8

Gene sequence:

>1536_bases
ATGAAAACTGCATTACTGAGTGTGTCAGATAAAACTGGAATCGTTGAATTTGCTAGAGGTTTAGTTAAGGCAGATTTTAG
AATCATATCAACGGGTGGAACTAAAAAAGTTTTGGAAGAAGCTGGATTATCTATTGTTAGTGTTGAAGCTATTACTAATT
TTCCAGAAATGTTGGACGGACGAGTTAAGACGCTACATCCTGCCATTCACGGAGGATTGTTAGCGCGCCGTGACTTACCG
GAACATTTGGCAGCCCTCAAAGAACATAATATTGATTTGATTGATCTAGTTTGTGTTAATCTTTATCCGTTTAAATCGAC
TATTATGCAAAATGGAGTGACGGAAGCAGAAGCAATTGAACAAATTGATATTGGCGGTCCTAGTATGTTACGCTCAGCGG
CTAAAAATTTTGCATCAGTTTTACCCGTAGTTGATCCTAAAGATTATCAACCAGTTTTAGCTGCACTAGCCCATCAGACG
GACAATGTTAAATTTCGGCGAGCGTTAGCTTTGAAGGTCTTTCAGCATACTGCTGCTTATGACACTTTGATTGCTCAATA
TCTTGGACAAAACGGTGAAATTTTTCCAGATGAACTTACCAAGACTTATACTAAAAAACAAGTAATGCGTTATGGCGAAA
ATAGTCATCAAAAAGCAGCCTTTTATGAAGATGCGCTACCTGTACCATTTTCGATTGCTCAAGCTCAACAATTGCACGGC
AAAGAATTGTCATACAATAACATCAAGGATGCTGATGCCGCTTTAAAAATGAGCGCCGAATTTAATCAACCAGCCGTGGT
TGCGGTTAAGCATATGAATCCATGTGGGATTGGATTAGGTCAAAATATTGAAGAAGCTTGGGACCGCGCTTATGAAGCAG
ACTCGATGTCAATTTTTGGTGGGATTATCGTCTTAAATCGACCAGTAGATTTGGCGACAGCAGAAAAAATGCATAAACTT
TTTCTTGAAATTATTATTGCACCTAGTTTTGAAAAAGAGGCTTTTACAGTTTTAGCTCAAAAAAAGAACTTACGGATTAT
GACGGTCGATTTTAATCAACATCAGGATGCTAAAGAACTTGAAACAGTTTCAGTAATGGGCGGGCTCTTGGTTCAAGAAC
AGGATGCGGTAGTTGAAACGGCGACTGACTTCAAAGTGGTTTCTAAACGTCAGCCAACGGAAAGTGAACTTAAGGCAATG
GTATTTGGTCAAACCGTTGTTAAACACGTTAAAAGTAATGCAATTGTGATTACAACTGATCAACAAACCTTAGGGATTGG
TGCAGGCCAAATGAATCGAATTGGTTCGGTTGAAATTGCGGTTAAACAGGCTGAAGGTTCAGCCAATTTTAAAAATGCGG
TGATGGCTTCGGATGCATTTTTCCCTATGGAAGATTGTGTTGAATATGCTGCTAAACATGGAATTAAAGCTATCGTTGAA
CCTGGTGGAAGTATCAAAGATCAAGCTTCAATTGATAAGGCGGATGAGTTAGGAATTTCATTAATATTTAGTGGACGTCG
TCACTTTAGACATTAA

Upstream 100 bases:

>100_bases
TAGCTCAACGTATCCATCAAGTTGAACATGATTTATATTTTAGAAGCATCTGCCAAGTATTAAAACAGAGAAAGTTGATT
AACTAAGGAGAGATTAAGCC

Downstream 100 bases:

>100_bases
GCGGAGGTACCAGATGGCAAAAGTACTTGTGGTTGGAAATGGTGCAAGGGAGCACGTTCTTGGACGTCAGCTATTGAAAA
GTAATCAAGTAGAAAAAGTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MKTALLSVSDKTGIVEFARGLVKADFRIISTGGTKKVLEEAGLSIVSVEAITNFPEMLDGRVKTLHPAIHGGLLARRDLP
EHLAALKEHNIDLIDLVCVNLYPFKSTIMQNGVTEAEAIEQIDIGGPSMLRSAAKNFASVLPVVDPKDYQPVLAALAHQT
DNVKFRRALALKVFQHTAAYDTLIAQYLGQNGEIFPDELTKTYTKKQVMRYGENSHQKAAFYEDALPVPFSIAQAQQLHG
KELSYNNIKDADAALKMSAEFNQPAVVAVKHMNPCGIGLGQNIEEAWDRAYEADSMSIFGGIIVLNRPVDLATAEKMHKL
FLEIIIAPSFEKEAFTVLAQKKNLRIMTVDFNQHQDAKELETVSVMGGLLVQEQDAVVETATDFKVVSKRQPTESELKAM
VFGQTVVKHVKSNAIVITTDQQTLGIGAGQMNRIGSVEIAVKQAEGSANFKNAVMASDAFFPMEDCVEYAAKHGIKAIVE
PGGSIKDQASIDKADELGISLIFSGRRHFRH

Sequences:

>Translated_511_residues
MKTALLSVSDKTGIVEFARGLVKADFRIISTGGTKKVLEEAGLSIVSVEAITNFPEMLDGRVKTLHPAIHGGLLARRDLP
EHLAALKEHNIDLIDLVCVNLYPFKSTIMQNGVTEAEAIEQIDIGGPSMLRSAAKNFASVLPVVDPKDYQPVLAALAHQT
DNVKFRRALALKVFQHTAAYDTLIAQYLGQNGEIFPDELTKTYTKKQVMRYGENSHQKAAFYEDALPVPFSIAQAQQLHG
KELSYNNIKDADAALKMSAEFNQPAVVAVKHMNPCGIGLGQNIEEAWDRAYEADSMSIFGGIIVLNRPVDLATAEKMHKL
FLEIIIAPSFEKEAFTVLAQKKNLRIMTVDFNQHQDAKELETVSVMGGLLVQEQDAVVETATDFKVVSKRQPTESELKAM
VFGQTVVKHVKSNAIVITTDQQTLGIGAGQMNRIGSVEIAVKQAEGSANFKNAVMASDAFFPMEDCVEYAAKHGIKAIVE
PGGSIKDQASIDKADELGISLIFSGRRHFRH
>Mature_511_residues
MKTALLSVSDKTGIVEFARGLVKADFRIISTGGTKKVLEEAGLSIVSVEAITNFPEMLDGRVKTLHPAIHGGLLARRDLP
EHLAALKEHNIDLIDLVCVNLYPFKSTIMQNGVTEAEAIEQIDIGGPSMLRSAAKNFASVLPVVDPKDYQPVLAALAHQT
DNVKFRRALALKVFQHTAAYDTLIAQYLGQNGEIFPDELTKTYTKKQVMRYGENSHQKAAFYEDALPVPFSIAQAQQLHG
KELSYNNIKDADAALKMSAEFNQPAVVAVKHMNPCGIGLGQNIEEAWDRAYEADSMSIFGGIIVLNRPVDLATAEKMHKL
FLEIIIAPSFEKEAFTVLAQKKNLRIMTVDFNQHQDAKELETVSVMGGLLVQEQDAVVETATDFKVVSKRQPTESELKAM
VFGQTVVKHVKSNAIVITTDQQTLGIGAGQMNRIGSVEIAVKQAEGSANFKNAVMASDAFFPMEDCVEYAAKHGIKAIVE
PGGSIKDQASIDKADELGISLIFSGRRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family

Homologues:

Organism=Homo sapiens, GI20127454, Length=474, Percent_Identity=37.1308016877637, Blast_Score=274, Evalue=2e-73,
Organism=Escherichia coli, GI1790439, Length=526, Percent_Identity=47.7186311787072, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI71985564, Length=609, Percent_Identity=31.8555008210181, Blast_Score=258, Evalue=7e-69,
Organism=Caenorhabditis elegans, GI71985574, Length=311, Percent_Identity=28.6173633440514, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=475, Percent_Identity=36.8421052631579, Blast_Score=277, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6323056, Length=472, Percent_Identity=36.6525423728814, Blast_Score=273, Evalue=7e-74,
Organism=Drosophila melanogaster, GI24649832, Length=601, Percent_Identity=32.4459234608985, Blast_Score=275, Evalue=7e-74,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR9_PEDPA (Q03E83)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804931.1
- ProteinModelPortal:   Q03E83
- SMR:   Q03E83
- STRING:   Q03E83
- GeneID:   4417281
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1458
- NMPDR:   fig|278197.10.peg.1283
- eggNOG:   COG0138
- HOGENOM:   HBG498048
- OMA:   ASDGFFP
- PhylomeDB:   Q03E83
- ProtClustDB:   PRK00881
- BioCyc:   PPEN278197:PEPE_1458-MONOMER
- HAMAP:   MF_00139
- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607
- Gene3D:   G3DSA:3.40.50.1380
- PANTHER:   PTHR11692
- PIRSF:   PIRSF000414
- SMART:   SM00798
- SMART:   SM00851
- TIGRFAMs:   TIGR00355

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS; SSF53927 Cytidine_deaminase-like; SSF52335 MGS-like_dom

EC number: =2.1.2.3; =3.5.4.10

Molecular weight: Translated: 55931; Mature: 55931

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTALLSVSDKTGIVEFARGLVKADFRIISTGGTKKVLEEAGLSIVSVEAITNFPEMLDG
CCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEEEHHHHCCHHHHCC
RVKTLHPAIHGGLLARRDLPEHLAALKEHNIDLIDLVCVNLYPFKSTIMQNGVTEAEAIE
CHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHH
QIDIGGPSMLRSAAKNFASVLPVVDPKDYQPVLAALAHQTDNVKFRRALALKVFQHTAAY
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DTLIAQYLGQNGEIFPDELTKTYTKKQVMRYGENSHQKAAFYEDALPVPFSIAQAQQLHG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCC
KELSYNNIKDADAALKMSAEFNQPAVVAVKHMNPCGIGLGQNIEEAWDRAYEADSMSIFG
CCCCCCCCCCHHHHEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEC
GIIVLNRPVDLATAEKMHKLFLEIIIAPSFEKEAFTVLAQKKNLRIMTVDFNQHQDAKEL
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCEEEEEECCCCCCHHHH
ETVSVMGGLLVQEQDAVVETATDFKVVSKRQPTESELKAMVFGQTVVKHVKSNAIVITTD
HHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
QQTLGIGAGQMNRIGSVEIAVKQAEGSANFKNAVMASDAFFPMEDCVEYAAKHGIKAIVE
CCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHCCCEEEEC
PGGSIKDQASIDKADELGISLIFSGRRHFRH
CCCCCCCCCCCCCHHHCCEEEEECCHHHCCC
>Mature Secondary Structure
MKTALLSVSDKTGIVEFARGLVKADFRIISTGGTKKVLEEAGLSIVSVEAITNFPEMLDG
CCCEEEEECCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHCCCEEEEEHHHHCCHHHHCC
RVKTLHPAIHGGLLARRDLPEHLAALKEHNIDLIDLVCVNLYPFKSTIMQNGVTEAEAIE
CHHHHHHHHHCCHHHHCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHCCCCHHHHHH
QIDIGGPSMLRSAAKNFASVLPVVDPKDYQPVLAALAHQTDNVKFRRALALKVFQHTAAY
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
DTLIAQYLGQNGEIFPDELTKTYTKKQVMRYGENSHQKAAFYEDALPVPFSIAQAQQLHG
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCHHHHHHHHHCC
KELSYNNIKDADAALKMSAEFNQPAVVAVKHMNPCGIGLGQNIEEAWDRAYEADSMSIFG
CCCCCCCCCCHHHHEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCEEEC
GIIVLNRPVDLATAEKMHKLFLEIIIAPSFEKEAFTVLAQKKNLRIMTVDFNQHQDAKEL
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHEECCCCEEEEEECCCCCCHHHH
ETVSVMGGLLVQEQDAVVETATDFKVVSKRQPTESELKAMVFGQTVVKHVKSNAIVITTD
HHHHHHHHHHEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEC
QQTLGIGAGQMNRIGSVEIAVKQAEGSANFKNAVMASDAFFPMEDCVEYAAKHGIKAIVE
CCEECCCCCCCCCCCCEEEEEEECCCCCCCCHHHEECCCCCCHHHHHHHHHHCCCEEEEC
PGGSIKDQASIDKADELGISLIFSGRRHFRH
CCCCCCCCCCCCCHHHCCEEEEECCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA