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The map label for this gene is cspLA [H]

Identifier: 116493183

GI number: 116493183

Start: 1419492

End: 1419692

Strand: Reverse

Name: cspLA [H]

Synonym: PEPE_1445

Alternate gene names: 116493183

Gene position: 1419692-1419492 (Counterclockwise)

Preceding gene: 116493184

Following gene: 116493182

Centisome position: 77.48

GC content: 41.29

Gene sequence:

>201_bases
ATGGAACAAGGTACAGTTAAATGGTTTAACGCTGATAAAGGTTTTGGTTTTATTACTCGCGAAGATGGTAGTGACGTATT
CGTTCACTTCTCAGCTATCCAAGGTGACGGATTCAAGACTCTTGAAGAAGGCCAAGCAGTTTCTTTCGACGTTGAAGAAA
GCGACCGTGGACCACAAGCAGTTAACGTAAACAAAAACTAA

Upstream 100 bases:

>100_bases
CGTTTCATTTTGAACTATCCCCATTACTACCGGCTCAAGCTACAAATTAAACAATGCCGTCTATTACTATATAGATTAGG
TTTACAGGAGGATGTTCATA

Downstream 100 bases:

>100_bases
TTTAAATTAAAATAGTTATGAGAGTAGGGAGCTTCGGCAACCTACTCTTTTTTTATGCAAAACATAAAGATTTAGTTAAA
TACTATTAATTAATTAGGTA

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: CspL [H]

Number of amino acids: Translated: 66; Mature: 66

Protein sequence:

>66_residues
MEQGTVKWFNADKGFGFITREDGSDVFVHFSAIQGDGFKTLEEGQAVSFDVEESDRGPQAVNVNKN

Sequences:

>Translated_66_residues
MEQGTVKWFNADKGFGFITREDGSDVFVHFSAIQGDGFKTLEEGQAVSFDVEESDRGPQAVNVNKN
>Mature_66_residues
MEQGTVKWFNADKGFGFITREDGSDVFVHFSAIQGDGFKTLEEGQAVSFDVEESDRGPQAVNVNKN

Specific function: Binds To And Stimulates The Transcription Of The CCAAT- Containing, Cold-Shock-Inducible Promoters Of The H-Ns And Gyra Proteins. Binds Also To The Inverted Repeat 5'-ATTGG-3'. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1789979, Length=62, Percent_Identity=70.9677419354839, Blast_Score=89, Evalue=4e-20,
Organism=Escherichia coli, GI1788126, Length=63, Percent_Identity=68.2539682539683, Blast_Score=87, Evalue=2e-19,
Organism=Escherichia coli, GI1786841, Length=62, Percent_Identity=67.741935483871, Blast_Score=85, Evalue=9e-19,
Organism=Escherichia coli, GI2367114, Length=61, Percent_Identity=67.2131147540984, Blast_Score=85, Evalue=1e-18,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=67.2131147540984, Blast_Score=84, Evalue=2e-18,
Organism=Escherichia coli, GI1787834, Length=61, Percent_Identity=65.5737704918033, Blast_Score=80, Evalue=3e-17,
Organism=Escherichia coli, GI1787107, Length=61, Percent_Identity=57.3770491803279, Blast_Score=75, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 7285; Mature: 7285

Theoretical pI: Translated: 4.18; Mature: 4.18

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQGTVKWFNADKGFGFITREDGSDVFVHFSAIQGDGFKTLEEGQAVSFDVEESDRGPQA
CCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCEE
VNVNKN
EEECCC
>Mature Secondary Structure
MEQGTVKWFNADKGFGFITREDGSDVFVHFSAIQGDGFKTLEEGQAVSFDVEESDRGPQA
CCCCCEEEEECCCCCEEEEECCCCEEEEEEEEEECCCCCCCCCCCEEEEECCCCCCCCEE
VNVNKN
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669; 9439003 [H]