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The map label for this gene is apbE [H]

Identifier: 116493095

GI number: 116493095

Start: 1334657

End: 1335598

Strand: Reverse

Name: apbE [H]

Synonym: PEPE_1349

Alternate gene names: 116493095

Gene position: 1335598-1334657 (Counterclockwise)

Preceding gene: 116493096

Following gene: 116493094

Centisome position: 72.89

GC content: 38.54

Gene sequence:

>942_bases
ATGGAGTTAGTTAATAAACAGCATCATGCTTTAGGAACGGTAATTAACCTTTCAATAGCCTCCATCTATCAAGTTGATAT
CTTAGAAAAAACTATAAAAATGATTGATGATTTCGAAGACCGTTTAACAGTTAATCGACCATCTTCGGAAGTGATGGAGA
TTAACCATCAAGCTGGAAAACAAGCTGTTCAAGTTTCGGAAACAACGTATCGCTTAATTGAAAAGGCGGTAGAAGTTAGT
AGATGGCAATTAGGTTTTAACGTGGCCATTGGGCCGCTAGTCAAATTGTGGAAAATTGGATTTGATGGGGCGAATAAACC
TAGTCATGCTCAAATTTTAGAACGGATAAAATTAATTGACCCGCAAGCAATTGTTTTGAACGCTCAAAATCGAAGTGTTT
TCCTTAAAAAAGAGGGAATGGAATTAGATTTAGGGGGGATTGCGAAAGGATTTATTGCTGACGAAATTAAGGCATATTGG
CAAAGCGAACACGTAGCGTCAGGAATTATTGATTTGGGCGGTAATGTGTTGTTAGTGGGGCGGAGTCAGCATCCCGATGC
AAAATGGAAAGTTGGGATTCAAAATCCAATTAAATCAAGGCACGTCCCGTTGGGAGTTCTACATATTCCAGAAAAGTCTG
TTGGCACATCAGGCATTTATGAACGGAAATTAATAGTTGAAGGGCAGACATATCATCACATGTTTGATTCAGCGACGGGA
TATCCAATTGCTAATAATTTAGCTAGCGTGTCCATAGTAAGCGATCGTTCGATTGATGGAGAAATTTGGTCAACCATTTG
TTTTTATCAAGGAATTGAAAAGGGCAAAGCGCTACTGGCCAATCAACCAGGAATTGAAGCAGTGTTTATTACACGGGATT
TGAAAGTTTATGTAACCGATGGACTACAGAATTGTTTCAATGAAATCGCGAATAATAGTTAA

Upstream 100 bases:

>100_bases
GCTTTTTTTATTGAAAATAATTTCCAAACTACAAAAGAAATCGTTTTCTAGGTAAGGTATAATATTAGATATAAATATAT
TGGGAAAAGGGGGCGGACGA

Downstream 100 bases:

>100_bases
TCCATATTTTTACATATAGAATGGGGAATATTTATGAAAAAATTTGTTGCAATCGTTGGAACGAATAGTAAAAAATCAAC
CAACCGTAAATTACTACAGT

Product: thiamine biosynthesis membrane-associated lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MELVNKQHHALGTVINLSIASIYQVDILEKTIKMIDDFEDRLTVNRPSSEVMEINHQAGKQAVQVSETTYRLIEKAVEVS
RWQLGFNVAIGPLVKLWKIGFDGANKPSHAQILERIKLIDPQAIVLNAQNRSVFLKKEGMELDLGGIAKGFIADEIKAYW
QSEHVASGIIDLGGNVLLVGRSQHPDAKWKVGIQNPIKSRHVPLGVLHIPEKSVGTSGIYERKLIVEGQTYHHMFDSATG
YPIANNLASVSIVSDRSIDGEIWSTICFYQGIEKGKALLANQPGIEAVFITRDLKVYVTDGLQNCFNEIANNS

Sequences:

>Translated_313_residues
MELVNKQHHALGTVINLSIASIYQVDILEKTIKMIDDFEDRLTVNRPSSEVMEINHQAGKQAVQVSETTYRLIEKAVEVS
RWQLGFNVAIGPLVKLWKIGFDGANKPSHAQILERIKLIDPQAIVLNAQNRSVFLKKEGMELDLGGIAKGFIADEIKAYW
QSEHVASGIIDLGGNVLLVGRSQHPDAKWKVGIQNPIKSRHVPLGVLHIPEKSVGTSGIYERKLIVEGQTYHHMFDSATG
YPIANNLASVSIVSDRSIDGEIWSTICFYQGIEKGKALLANQPGIEAVFITRDLKVYVTDGLQNCFNEIANNS
>Mature_313_residues
MELVNKQHHALGTVINLSIASIYQVDILEKTIKMIDDFEDRLTVNRPSSEVMEINHQAGKQAVQVSETTYRLIEKAVEVS
RWQLGFNVAIGPLVKLWKIGFDGANKPSHAQILERIKLIDPQAIVLNAQNRSVFLKKEGMELDLGGIAKGFIADEIKAYW
QSEHVASGIIDLGGNVLLVGRSQHPDAKWKVGIQNPIKSRHVPLGVLHIPEKSVGTSGIYERKLIVEGQTYHHMFDSATG
YPIANNLASVSIVSDRSIDGEIWSTICFYQGIEKGKALLANQPGIEAVFITRDLKVYVTDGLQNCFNEIANNS

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=300, Percent_Identity=24.3333333333333, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34677; Mature: 34677

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELVNKQHHALGTVINLSIASIYQVDILEKTIKMIDDFEDRLTVNRPSSEVMEINHQAGK
CCCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCHHHHHHHHHHCH
QAVQVSETTYRLIEKAVEVSRWQLGFNVAIGPLVKLWKIGFDGANKPSHAQILERIKLID
HHEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
PQAIVLNAQNRSVFLKKEGMELDLGGIAKGFIADEIKAYWQSEHVASGIIDLGGNVLLVG
CCEEEEECCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
RSQHPDAKWKVGIQNPIKSRHVPLGVLHIPEKSVGTSGIYERKLIVEGQTYHHMFDSATG
CCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHCCCCC
YPIANNLASVSIVSDRSIDGEIWSTICFYQGIEKGKALLANQPGIEAVFITRDLKVYVTD
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEECEEEEEEH
GLQNCFNEIANNS
HHHHHHHHHHCCC
>Mature Secondary Structure
MELVNKQHHALGTVINLSIASIYQVDILEKTIKMIDDFEDRLTVNRPSSEVMEINHQAGK
CCCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCHHHHEEECCCCHHHHHHHHHHCH
QAVQVSETTYRLIEKAVEVSRWQLGFNVAIGPLVKLWKIGFDGANKPSHAQILERIKLID
HHEEHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
PQAIVLNAQNRSVFLKKEGMELDLGGIAKGFIADEIKAYWQSEHVASGIIDLGGNVLLVG
CCEEEEECCCCEEEEEECCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
RSQHPDAKWKVGIQNPIKSRHVPLGVLHIPEKSVGTSGIYERKLIVEGQTYHHMFDSATG
CCCCCCCEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHCCCCC
YPIANNLASVSIVSDRSIDGEIWSTICFYQGIEKGKALLANQPGIEAVFITRDLKVYVTD
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCEEECCCCCCEEEEEEECEEEEEEH
GLQNCFNEIANNS
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]