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The map label for this gene is pox5 [H]

Identifier: 116493000

GI number: 116493000

Start: 1238398

End: 1240146

Strand: Reverse

Name: pox5 [H]

Synonym: PEPE_1252

Alternate gene names: 116493000

Gene position: 1240146-1238398 (Counterclockwise)

Preceding gene: 116493001

Following gene: 116492998

Centisome position: 67.68

GC content: 38.88

Gene sequence:

>1749_bases
ATGGCAGAAAAAATTCAAGCAGGAATAGCAATGTTAAAAGTAATTGAATCATGGGGTGTAGACCATATTTACGGTTACCC
TGGGGGATCAATTAATTCAACTATGGATTCTTTGCAAAAAGAACAAGACAATATTAAATACATCCAAGTTCGACATGAAC
AAGTCGGGGCACTTGCAGCAGCAGCTGACGCCAAATTGACTGGTAAAATTGGGGTAGCTTTTGGGTCAGCAGGACCCGGC
GCAGTCAACCTACTTAATGGATTGTATGATGCAAAGGAAGACCATGTTCCTGTTTTGGCAATTGTAGGACAAGTCCCACA
TACCCGAATGAATTATGATTACTTCCAAGAATTCAATGAATCTCCTATGTTCGATGACGTTGCGGTTTACAATCGAATTG
TGATGACCCCAGAAAGTTTGCCATATGTAATTGATAAAGCGATTCGTATGGCATATAAAGAAAAGGGAGTAGCGGTAGTT
ATTATTCCGAATGACTTTGGCTACGTAGAAATCCCAACTGGCAATTATTCTACTGAAAAAACACGTAATCAACCTGAGAT
TAAACCTCAACCTGATTCTGAATTAGTGCAAGATGCTTGGGATATGATTAAAAATGCTAAATATCCAGTTATTCATTTAG
GACAAGGTGCTCGCGGAGCCACCAAAGAAGTTGAGGCATTATCTGAAAAACTTCAAATTCCAATCGTAATTACTGGGTTA
GCTAAGGGCGTAATCCCTGATGAATATGAGGGGAATATGGGGCCATTTAACCGAGCGGCTTCTAAGGTTTCTAATGAATT
AATTAAGAAAACGGACCTTTTAATTTCAATTGGTGCTAACTTCCCATTTGCAGCAATTGTATATGGAGATCGACCATTTA
AGTATATTCAAGTTGATAATGATCAAGCTATTTTTGGGCGCCATCATTCACTTGATTTAGGAATTTGGGCAGATGCTAAA
TTATTCGTTGAAAAATTGGATGAAATTAGCGAAGCCGTTCAACCGCGTCCATTCTACAAAGCGGCTGTGGCTGCCCAAAA
AGACTGGCGTAAATACCTAAAGGGCTTGATGAATCGGGCAGATGGACCTCTAGAATACGAACAAGTTTATCAACAAATTA
ACCGAATTGCAGATGACGACGCCGTATTTTCAATGGATGTTGGGGATAATATTATTAATAGTTTCCGTTTCCTTGATATG
AATCCTAAACAAAAAATCTTAACTTCAGCTTTGTTTGCAACTATGGGTTGTGGAATTCCTGGAGCAATGGCTAGCAAGTT
AAGCTATCCAGATCGTCAAGTATTCAATATTGCTGGAGATGGAGCGTTCTCAATGGTAATGCAGGATATGATTACCGAAA
AACGTTATAACTTACCAATCATTAACATCGTTACATCTAACCGCCATCTAAGTTTTATTAAGTCTGAACAAGATGACGTT
CCTCAAGATGAATTCGGGGTAGATTTAACTGATGCTGACTACGCTAAAATTGCCGAAGGAATGGGTGTTAAAGGAATTCG
AGTCGATGAAATTAGTCAGCTTGAAAAGGCCTTTGATGATGCTGTTGCAGAAGTAAAAGCTGGTCGTCCGGTGTTAATTG
ATGCCAATATCACGGATAAACGAGGGATTCCAGTTGAACTTGATTTGGAACAGGATGATACATTGTATACACCTGAATTT
CTTAAAGAATGGGATGCAGAAGAATTGAAGCCATTTGATGAATATTTAGCTCAATATTCTGCCGAATAA

Upstream 100 bases:

>100_bases
TTTACTCGGTAAGCATATTTATTGATTTTTACTTATAAAAGATAAATAATCAGATATGAAAAACGAAAACGTTTTCAAAA
AGCTGAGGAGGAAATTATTC

Downstream 100 bases:

>100_bases
TTAGGCTATATAAAAAGCGCTTCGGCTTAGAACCGAAGTGCTTTTTGGTAGAAATATTATAGATATCTAAGTTCTTTTGA
AGTATGGGTAAATGGTAGGC

Product: pyruvate oxidase

Products: NA

Alternate protein names: Pyruvic oxidase; POX [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAEKIQAGIAMLKVIESWGVDHIYGYPGGSINSTMDSLQKEQDNIKYIQVRHEQVGALAAAADAKLTGKIGVAFGSAGPG
AVNLLNGLYDAKEDHVPVLAIVGQVPHTRMNYDYFQEFNESPMFDDVAVYNRIVMTPESLPYVIDKAIRMAYKEKGVAVV
IIPNDFGYVEIPTGNYSTEKTRNQPEIKPQPDSELVQDAWDMIKNAKYPVIHLGQGARGATKEVEALSEKLQIPIVITGL
AKGVIPDEYEGNMGPFNRAASKVSNELIKKTDLLISIGANFPFAAIVYGDRPFKYIQVDNDQAIFGRHHSLDLGIWADAK
LFVEKLDEISEAVQPRPFYKAAVAAQKDWRKYLKGLMNRADGPLEYEQVYQQINRIADDDAVFSMDVGDNIINSFRFLDM
NPKQKILTSALFATMGCGIPGAMASKLSYPDRQVFNIAGDGAFSMVMQDMITEKRYNLPIINIVTSNRHLSFIKSEQDDV
PQDEFGVDLTDADYAKIAEGMGVKGIRVDEISQLEKAFDDAVAEVKAGRPVLIDANITDKRGIPVELDLEQDDTLYTPEF
LKEWDAEELKPFDEYLAQYSAE

Sequences:

>Translated_582_residues
MAEKIQAGIAMLKVIESWGVDHIYGYPGGSINSTMDSLQKEQDNIKYIQVRHEQVGALAAAADAKLTGKIGVAFGSAGPG
AVNLLNGLYDAKEDHVPVLAIVGQVPHTRMNYDYFQEFNESPMFDDVAVYNRIVMTPESLPYVIDKAIRMAYKEKGVAVV
IIPNDFGYVEIPTGNYSTEKTRNQPEIKPQPDSELVQDAWDMIKNAKYPVIHLGQGARGATKEVEALSEKLQIPIVITGL
AKGVIPDEYEGNMGPFNRAASKVSNELIKKTDLLISIGANFPFAAIVYGDRPFKYIQVDNDQAIFGRHHSLDLGIWADAK
LFVEKLDEISEAVQPRPFYKAAVAAQKDWRKYLKGLMNRADGPLEYEQVYQQINRIADDDAVFSMDVGDNIINSFRFLDM
NPKQKILTSALFATMGCGIPGAMASKLSYPDRQVFNIAGDGAFSMVMQDMITEKRYNLPIINIVTSNRHLSFIKSEQDDV
PQDEFGVDLTDADYAKIAEGMGVKGIRVDEISQLEKAFDDAVAEVKAGRPVLIDANITDKRGIPVELDLEQDDTLYTPEF
LKEWDAEELKPFDEYLAQYSAE
>Mature_581_residues
AEKIQAGIAMLKVIESWGVDHIYGYPGGSINSTMDSLQKEQDNIKYIQVRHEQVGALAAAADAKLTGKIGVAFGSAGPGA
VNLLNGLYDAKEDHVPVLAIVGQVPHTRMNYDYFQEFNESPMFDDVAVYNRIVMTPESLPYVIDKAIRMAYKEKGVAVVI
IPNDFGYVEIPTGNYSTEKTRNQPEIKPQPDSELVQDAWDMIKNAKYPVIHLGQGARGATKEVEALSEKLQIPIVITGLA
KGVIPDEYEGNMGPFNRAASKVSNELIKKTDLLISIGANFPFAAIVYGDRPFKYIQVDNDQAIFGRHHSLDLGIWADAKL
FVEKLDEISEAVQPRPFYKAAVAAQKDWRKYLKGLMNRADGPLEYEQVYQQINRIADDDAVFSMDVGDNIINSFRFLDMN
PKQKILTSALFATMGCGIPGAMASKLSYPDRQVFNIAGDGAFSMVMQDMITEKRYNLPIINIVTSNRHLSFIKSEQDDVP
QDEFGVDLTDADYAKIAEGMGVKGIRVDEISQLEKAFDDAVAEVKAGRPVLIDANITDKRGIPVELDLEQDDTLYTPEFL
KEWDAEELKPFDEYLAQYSAE

Specific function: Important for the aerobic growth. Decarboxylates pyruvate in four steps. The energy released is partially stored in acetyl phosphate [H]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=26.3157894736842, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI21361361, Length=592, Percent_Identity=24.1554054054054, Blast_Score=123, Evalue=5e-28,
Organism=Escherichia coli, GI1787096, Length=552, Percent_Identity=32.0652173913043, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790104, Length=472, Percent_Identity=30.7203389830508, Blast_Score=215, Evalue=8e-57,
Organism=Escherichia coli, GI87081685, Length=556, Percent_Identity=25.1798561151079, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786717, Length=555, Percent_Identity=25.5855855855856, Blast_Score=182, Evalue=7e-47,
Organism=Escherichia coli, GI1788716, Length=499, Percent_Identity=22.4448897795591, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17542570, Length=586, Percent_Identity=24.2320819112628, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17531299, Length=569, Percent_Identity=24.6045694200351, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531301, Length=569, Percent_Identity=24.6045694200351, Blast_Score=118, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323755, Length=557, Percent_Identity=24.5960502692998, Blast_Score=156, Evalue=7e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=546, Percent_Identity=26.1904761904762, Blast_Score=143, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6320123, Length=551, Percent_Identity=20.6896551724138, Blast_Score=68, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323163, Length=489, Percent_Identity=22.9038854805726, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321524, Length=475, Percent_Identity=20.6315789473684, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19922626, Length=581, Percent_Identity=25.6454388984509, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014092
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.3.3 [H]

Molecular weight: Translated: 64695; Mature: 64564

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEKIQAGIAMLKVIESWGVDHIYGYPGGSINSTMDSLQKEQDNIKYIQVRHEQVGALAA
CCHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
AADAKLTGKIGVAFGSAGPGAVNLLNGLYDAKEDHVPVLAIVGQVPHTRMNYDYFQEFNE
HCCCCEECEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCC
SPMFDDVAVYNRIVMTPESLPYVIDKAIRMAYKEKGVAVVIIPNDFGYVEIPTGNYSTEK
CCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCC
TRNQPEIKPQPDSELVQDAWDMIKNAKYPVIHLGQGARGATKEVEALSEKLQIPIVITGL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEC
AKGVIPDEYEGNMGPFNRAASKVSNELIKKTDLLISIGANFPFAAIVYGDRPFKYIQVDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCEEEEEECC
DQAIFGRHHSLDLGIWADAKLFVEKLDEISEAVQPRPFYKAAVAAQKDWRKYLKGLMNRA
CCEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DGPLEYEQVYQQINRIADDDAVFSMDVGDNIINSFRFLDMNPKQKILTSALFATMGCGIP
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCEEEECCCHHHHHHHHHHHHHHCCCCC
GAMASKLSYPDRQVFNIAGDGAFSMVMQDMITEKRYNLPIINIVTSNRHLSFIKSEQDDV
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCC
PQDEFGVDLTDADYAKIAEGMGVKGIRVDEISQLEKAFDDAVAEVKAGRPVLIDANITDK
CCHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
RGIPVELDLEQDDTLYTPEFLKEWDAEELKPFDEYLAQYSAE
CCCCEEEECCCCCCEECHHHHHHCCHHHCCHHHHHHHHHCCC
>Mature Secondary Structure 
AEKIQAGIAMLKVIESWGVDHIYGYPGGSINSTMDSLQKEQDNIKYIQVRHEQVGALAA
CHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHH
AADAKLTGKIGVAFGSAGPGAVNLLNGLYDAKEDHVPVLAIVGQVPHTRMNYDYFQEFNE
HCCCCEECEEEEEECCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCHHHHHHCCC
SPMFDDVAVYNRIVMTPESLPYVIDKAIRMAYKEKGVAVVIIPNDFGYVEIPTGNYSTEK
CCCCCHHHHHHEEEECCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCEEEECCCCCCCCC
TRNQPEIKPQPDSELVQDAWDMIKNAKYPVIHLGQGARGATKEVEALSEKLQIPIVITGL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCEEEEEC
AKGVIPDEYEGNMGPFNRAASKVSNELIKKTDLLISIGANFPFAAIVYGDRPFKYIQVDN
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEECCCCEEEEEECC
DQAIFGRHHSLDLGIWADAKLFVEKLDEISEAVQPRPFYKAAVAAQKDWRKYLKGLMNRA
CCEEECCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
DGPLEYEQVYQQINRIADDDAVFSMDVGDNIINSFRFLDMNPKQKILTSALFATMGCGIP
CCCCHHHHHHHHHHHHCCCCCEEEECCCHHHHHCEEEECCCHHHHHHHHHHHHHHCCCCC
GAMASKLSYPDRQVFNIAGDGAFSMVMQDMITEKRYNLPIINIVTSNRHLSFIKSEQDDV
HHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCC
PQDEFGVDLTDADYAKIAEGMGVKGIRVDEISQLEKAFDDAVAEVKAGRPVLIDANITDK
CCHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
RGIPVELDLEQDDTLYTPEFLKEWDAEELKPFDEYLAQYSAE
CCCCEEEECCCCCCEECHHHHHHCCHHHCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12566566; 8145244; 8438155 [H]