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The map label for this gene is purB [H]

Identifier: 116492994

GI number: 116492994

Start: 1231423

End: 1232721

Strand: Reverse

Name: purB [H]

Synonym: PEPE_1246

Alternate gene names: 116492994

Gene position: 1232721-1231423 (Counterclockwise)

Preceding gene: 116492996

Following gene: 116492993

Centisome position: 67.27

GC content: 37.03

Gene sequence:

>1299_bases
ATGATTGATCGTTATGCAAGACCCGAAATGCAAAAAATTTGGAACCGCCAGTCCCAATTTCAAAGTTGGCTTGACGTTGA
AATAGCCGTTGTTAAAGCTTGGAATAAGGATGGAAAGGTTCCTGATCAAGATCTCGAACTAATCGAAAAGAAAGCTAAGT
TTGATTTAGATCGAATTGATGAGCTTGAAAGTCAAACGCATCACGATGTAATTGCCTTTACTAGGTCCGTTTCCGAATCT
TTAGGCGAAGAGCGAAAATGGATTCATTACGGATTAACCAGTACAGACGTTGTCGATACAGCGCAGTCGCTTCGACTCAA
AGCTGCTAATGAAATTATTTCGGCAGATTTAGAAACACTAAAAACAACCTTGAAAGAACTATCAATCAAATATAAAGATT
TAATTGCCGTTGGACGGACTCATGGAATTCATGCCGAACCAACTACAATTGGATTAAAATTTTTACGCTGGTATTCAGAA
ATTAATCGTAATATTGAACGTTTTAATCATGCCCGTAAAAACATCGAAGTTGGCAAGATTAGTGGGGCAGTGGGGACTTT
TGCTAACATTACTCCTCAAATTGAAGAATGGGTTTGCCAAATGCTTGGAATTGGGCATCAAGAAATTTCGACTCAAATCC
TCCCACGTGATTTACACACAGAATATTTGAGTGTTTTGGCCTTAATCGCCTCCCAAATTGAAGAAATTGCAACTGAAATT
AGACATTTACAGCGTACAGAAGTCAATGAAATGCGAGAAGGATTTGCTAAAGGACAAAAAGGTTCATCAGCGATGCCTCA
TAAACGTAATCCTATTGGTTCTGAAAATGTTTCAGGACTAAGTCGAGTTGTTCGTGGAGAAATGATAACAGCGTTTGAAA
ATATGAATTTATGGCATGAACGAGACATTTCTCATTCGTCTGCTGAACGTGTAATTTTACCAGATTCAACAGCTTTAGTT
GACTACATTCTTACACGAATGAATCGGATTTTAAATAACTTGGTAGTTAATGAAGATGAAGTTCAAAAAGACCTTGAAAT
GACTCATGGATTGATTTTTTCTCAACAAGTTATGTTGAAATTGATTGATAAAGGTTGGACCCGCGAGGAAGCCTACGATA
CAGTTCAGCCAATCGCTATGATGGTATGGAATACTGGGCAACATTTTATGGATTTACTACTTGAAAATGATAAAATTAAG
TCAATTCTTGATGATGAAGAAATTCGAGCTATTTTTAACTATCAATACCATCTTAAAAACGTTGATGCAATCTACCAACG
GTGTGGTATCGAAGCATAA

Upstream 100 bases:

>100_bases
ATTATACAAAAGGTAATTCTTTTAATGGTTCGTGTTTATTAATACTGAAGATTTTGGTAAAATGGAGAGGTAAATATTTT
TAATTCAAAGGAGTAATCAA

Downstream 100 bases:

>100_bases
TTTAGATTAAAAGGGATGAGGAATTTTGATGAAAAAATCAAATGAAGCGTTACTTGTAATTGATTATACGAATGATTTTG
TGGCATCGAATGGTGCGTTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIDRYARPEMQKIWNRQSQFQSWLDVEIAVVKAWNKDGKVPDQDLELIEKKAKFDLDRIDELESQTHHDVIAFTRSVSES
LGEERKWIHYGLTSTDVVDTAQSLRLKAANEIISADLETLKTTLKELSIKYKDLIAVGRTHGIHAEPTTIGLKFLRWYSE
INRNIERFNHARKNIEVGKISGAVGTFANITPQIEEWVCQMLGIGHQEISTQILPRDLHTEYLSVLALIASQIEEIATEI
RHLQRTEVNEMREGFAKGQKGSSAMPHKRNPIGSENVSGLSRVVRGEMITAFENMNLWHERDISHSSAERVILPDSTALV
DYILTRMNRILNNLVVNEDEVQKDLEMTHGLIFSQQVMLKLIDKGWTREEAYDTVQPIAMMVWNTGQHFMDLLLENDKIK
SILDDEEIRAIFNYQYHLKNVDAIYQRCGIEA

Sequences:

>Translated_432_residues
MIDRYARPEMQKIWNRQSQFQSWLDVEIAVVKAWNKDGKVPDQDLELIEKKAKFDLDRIDELESQTHHDVIAFTRSVSES
LGEERKWIHYGLTSTDVVDTAQSLRLKAANEIISADLETLKTTLKELSIKYKDLIAVGRTHGIHAEPTTIGLKFLRWYSE
INRNIERFNHARKNIEVGKISGAVGTFANITPQIEEWVCQMLGIGHQEISTQILPRDLHTEYLSVLALIASQIEEIATEI
RHLQRTEVNEMREGFAKGQKGSSAMPHKRNPIGSENVSGLSRVVRGEMITAFENMNLWHERDISHSSAERVILPDSTALV
DYILTRMNRILNNLVVNEDEVQKDLEMTHGLIFSQQVMLKLIDKGWTREEAYDTVQPIAMMVWNTGQHFMDLLLENDKIK
SILDDEEIRAIFNYQYHLKNVDAIYQRCGIEA
>Mature_432_residues
MIDRYARPEMQKIWNRQSQFQSWLDVEIAVVKAWNKDGKVPDQDLELIEKKAKFDLDRIDELESQTHHDVIAFTRSVSES
LGEERKWIHYGLTSTDVVDTAQSLRLKAANEIISADLETLKTTLKELSIKYKDLIAVGRTHGIHAEPTTIGLKFLRWYSE
INRNIERFNHARKNIEVGKISGAVGTFANITPQIEEWVCQMLGIGHQEISTQILPRDLHTEYLSVLALIASQIEEIATEI
RHLQRTEVNEMREGFAKGQKGSSAMPHKRNPIGSENVSGLSRVVRGEMITAFENMNLWHERDISHSSAERVILPDSTALV
DYILTRMNRILNNLVVNEDEVQKDLEMTHGLIFSQQVMLKLIDKGWTREEAYDTVQPIAMMVWNTGQHFMDLLLENDKIK
SILDDEEIRAIFNYQYHLKNVDAIYQRCGIEA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=391, Percent_Identity=25.3196930946292, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=25.8145363408521, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1787376, Length=313, Percent_Identity=30.0319488817891, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17508577, Length=341, Percent_Identity=28.1524926686217, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32564234, Length=304, Percent_Identity=28.2894736842105, Blast_Score=105, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323391, Length=429, Percent_Identity=26.5734265734266, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324993, Length=205, Percent_Identity=28.780487804878, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24647570, Length=398, Percent_Identity=22.8643216080402, Blast_Score=115, Evalue=8e-26,
Organism=Drosophila melanogaster, GI78710009, Length=192, Percent_Identity=28.6458333333333, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49735; Mature: 49735

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDRYARPEMQKIWNRQSQFQSWLDVEIAVVKAWNKDGKVPDQDLELIEKKAKFDLDRID
CCCCCCCCHHHHHHCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHH
ELESQTHHDVIAFTRSVSESLGEERKWIHYGLTSTDVVDTAQSLRLKAANEIISADLETL
HHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTTLKELSIKYKDLIAVGRTHGIHAEPTTIGLKFLRWYSEINRNIERFNHARKNIEVGKI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
SGAVGTFANITPQIEEWVCQMLGIGHQEISTQILPRDLHTEYLSVLALIASQIEEIATEI
CCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVNEMREGFAKGQKGSSAMPHKRNPIGSENVSGLSRVVRGEMITAFENMNLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
RDISHSSAERVILPDSTALVDYILTRMNRILNNLVVNEDEVQKDLEMTHGLIFSQQVMLK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LIDKGWTREEAYDTVQPIAMMVWNTGQHFMDLLLENDKIKSILDDEEIRAIFNYQYHLKN
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
VDAIYQRCGIEA
HHHHHHHHCCCC
>Mature Secondary Structure
MIDRYARPEMQKIWNRQSQFQSWLDVEIAVVKAWNKDGKVPDQDLELIEKKAKFDLDRID
CCCCCCCCHHHHHHCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHCCHHHHH
ELESQTHHDVIAFTRSVSESLGEERKWIHYGLTSTDVVDTAQSLRLKAANEIISADLETL
HHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTTLKELSIKYKDLIAVGRTHGIHAEPTTIGLKFLRWYSEINRNIERFNHARKNIEVGKI
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
SGAVGTFANITPQIEEWVCQMLGIGHQEISTQILPRDLHTEYLSVLALIASQIEEIATEI
CCHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RHLQRTEVNEMREGFAKGQKGSSAMPHKRNPIGSENVSGLSRVVRGEMITAFENMNLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHH
RDISHSSAERVILPDSTALVDYILTRMNRILNNLVVNEDEVQKDLEMTHGLIFSQQVMLK
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LIDKGWTREEAYDTVQPIAMMVWNTGQHFMDLLLENDKIKSILDDEEIRAIFNYQYHLKN
HHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
VDAIYQRCGIEA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12397186 [H]