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The map label for this gene is pncA [H]

Identifier: 116492993

GI number: 116492993

Start: 1230837

End: 1231394

Strand: Reverse

Name: pncA [H]

Synonym: PEPE_1245

Alternate gene names: 116492993

Gene position: 1231394-1230837 (Counterclockwise)

Preceding gene: 116492994

Following gene: 116492992

Centisome position: 67.2

GC content: 38.35

Gene sequence:

>558_bases
ATGAAAAAATCAAATGAAGCGTTACTTGTAATTGATTATACGAATGATTTTGTGGCATCGAATGGTGCGTTAACTACAGG
AGTAGTTGGACAAAAGATTGAATCAGAGATTATCAAGTTGGCGAACCAAGAGCTACGTGAAGACGGTTGGGTGTTTTTGC
CAACTGATGTACATGTAGAAGGCGATCCATATCATCCAGAAACTAAGTTATTTCCTGCACACAATCTCAAAGGAACATGG
GGACGTGATTTTTACGGGCAGTTGCAGGATTGGTACGATTTAAATAAAGCTAATCAACGAGTTAAAATCCTTGATAAAAC
TCGTTATAGTGCTTTTACTGGAACGGACCTAGATATGCAACTAAGAGCTCGTAAAATTGATACAGTACGGCTAGTGGGGG
TATGTACGGATATTTGTATCCTTCATACTGCCGTTGATGCATATAATTTAGGCTATCGAATTATTATTAATGAAAAAGGT
GTGGCAACCTTTACAGAACATGGTCAAGAATGGGCACTAGAACATTTTAAGAATGCACTAGGTGCTCAGGTTGAATAG

Upstream 100 bases:

>100_bases
GCTATTTTTAACTATCAATACCATCTTAAAAACGTTGATGCAATCTACCAACGGTGTGGTATCGAAGCATAATTTAGATT
AAAAGGGATGAGGAATTTTG

Downstream 100 bases:

>100_bases
GATTAATATAAATGGGAACCGCTGAGAAAATTATTTTTTCAGGGGTTCCTTTTTATGTGGTAGTCAAAAATCAGCCTAAT
TTATGATAGAATGAAGTATT

Product: amidase

Products: nicotinate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKKSNEALLVIDYTNDFVASNGALTTGVVGQKIESEIIKLANQELREDGWVFLPTDVHVEGDPYHPETKLFPAHNLKGTW
GRDFYGQLQDWYDLNKANQRVKILDKTRYSAFTGTDLDMQLRARKIDTVRLVGVCTDICILHTAVDAYNLGYRIIINEKG
VATFTEHGQEWALEHFKNALGAQVE

Sequences:

>Translated_185_residues
MKKSNEALLVIDYTNDFVASNGALTTGVVGQKIESEIIKLANQELREDGWVFLPTDVHVEGDPYHPETKLFPAHNLKGTW
GRDFYGQLQDWYDLNKANQRVKILDKTRYSAFTGTDLDMQLRARKIDTVRLVGVCTDICILHTAVDAYNLGYRIIINEKG
VATFTEHGQEWALEHFKNALGAQVE
>Mature_185_residues
MKKSNEALLVIDYTNDFVASNGALTTGVVGQKIESEIIKLANQELREDGWVFLPTDVHVEGDPYHPETKLFPAHNLKGTW
GRDFYGQLQDWYDLNKANQRVKILDKTRYSAFTGTDLDMQLRARKIDTVRLVGVCTDICILHTAVDAYNLGYRIIINEKG
VATFTEHGQEWALEHFKNALGAQVE

Specific function: Unknown

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.19

Molecular weight: Translated: 20957; Mature: 20957

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSNEALLVIDYTNDFVASNGALTTGVVGQKIESEIIKLANQELREDGWVFLPTDVHVE
CCCCCCEEEEEECCCCEEECCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEC
GDPYHPETKLFPAHNLKGTWGRDFYGQLQDWYDLNKANQRVKILDKTRYSAFTGTDLDMQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCEE
LRARKIDTVRLVGVCTDICILHTAVDAYNLGYRIIINEKGVATFTEHGQEWALEHFKNAL
ECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHCCHHHHHHHHHHHH
GAQVE
CCCCC
>Mature Secondary Structure
MKKSNEALLVIDYTNDFVASNGALTTGVVGQKIESEIIKLANQELREDGWVFLPTDVHVE
CCCCCCEEEEEECCCCEEECCCCEEECHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEC
GDPYHPETKLFPAHNLKGTWGRDFYGQLQDWYDLNKANQRVKILDKTRYSAFTGTDLDMQ
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCEECCCCCCEE
LRARKIDTVRLVGVCTDICILHTAVDAYNLGYRIIINEKGVATFTEHGQEWALEHFKNAL
ECCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEHHHCCHHHHHHHHHHHH
GAQVE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nicotinamide; H2O

Specific reaction: nicotinamide + H2O = nicotinate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]