Click here to switch to the map view.

The map label for this gene is proS

Identifier: 116492650

GI number: 116492650

Start: 882678

End: 884393

Strand: Direct

Name: proS

Synonym: PEPE_0884

Alternate gene names: 116492650

Gene position: 882678-884393 (Clockwise)

Preceding gene: 116492649

Following gene: 116492651

Centisome position: 48.17

GC content: 38.64

Gene sequence:

>1716_bases
ATGAAACAATCAAAAATTCTTATTTCTACTGTAAAGGAAGTCCCAAATGATGCCGAAGTTTTAAGTCATAAAATGATGCT
TCGGGCAGGATACATCCGTCAAATTTCGGCCGGAATGTATGCATACTTACCATTAGCTTATAAAGTGATTAGTAAGATTG
AAAAAATCGTGCGTGAAGAAATGGAAGCAATCGACGCCGTTGAAATGTTAACTCCAGCGGTTTTACCAGCTGAATTATGG
AAGCAATCAGGCCGTTATGAAACATATGGTCAAGAACTCTATAAATTTAAGAATCGTCATGATCGTGATTTTATTCTTGG
ACCAACACACGAAGAAACCATGACTACATTGATTCGAGACGAAGTTAAATCATACAAGAAACTTCCACTTTCTTTATACC
AAATCCAAATGAAATATCGCGATGAAGATCGTCCTCGTTATGGATTACTACGCGGCCGTGAATTTTTAATGAAAGATGCT
TATTCTTTCCACGCTGATGAAGAAACGTTAGACCAAAGTTTCCGTGATTTCGAAAAAGCTTACCAAAACATTTTTAGACG
TTGTGGTCTTAATTTCCGTGAAATCGTGGGTGACGCCGGAGCAATGGGTGGTCGAGATTCCAAAGAATTTTCAGCTATTG
CTTCTATTGGTGAAGATACAATCGCTTACTCAGAAGAAAGCGATTATGCTGCTAACTTAGAAATGGCAAGTAGTGTCTAC
ACAGATCTTCAAATGCATGAAAATCAAGAAGAACTTACTAAAGTGGCTACTGATGATGCGCATTCAATTGATGAAGTTGC
TGCTAAGCTAGATGTGGATAGCAACCGTTTAATTAAAACTATGGTTTTAATTGTCGATGAAGCACCTGTTTTGGCTTTAC
TACGTGGTAATGATCAACTAAATGAAGTTAAGCTTACAAATCTTTTGCACGCTGATGAGGTTCGAGAAGCAACGGAGGAA
GAAGCCTTTGAACTTCTAGGTGCGCACGTGGGTTCGCTTGGTCCAGTACTTGAAAACAAACCAGAAAATTTAAAGATTGT
AGCAGATAAATATATTGAACAAATGGTTAACAGCGTTGTTGGTGCTAATGAAGATGGTTTCCATTTGAAAAATGTTAATG
TTGAACGCGATTTTACCGTTGATGAATATGCTGATATTCGTACTGTCCGTGAAGGCGAACTTGCTCCGGATGGTAAGGGT
GCTTTGAAATTCACTAAGGGAATCGAAATTGGTCATATCTTCAAGATTGGAACAAAGTACTCTGAAGCTTTGGATGCTAA
GGTACTAGATGAAAACGGACGAGCAATCCCAGTAATTATGGGTTGTTATGGAATTGGGGTGAGTCGTTTACTATCAGCTG
TTTCTGAACAACAATCGGATGAAAATGGGCTTGTATGGCCTAAGAATATTGCTCCTTATGATGTCCATGTAATTCCAGTT
AATGCCAAGAATGCGGAACAAATGGAAATTGCTGACCAAATTAACGGAGAACTAACTAAAGCAGGTTACGATGTATTAGT
AGATGATCGTAAGGAACGTGCCGGAGTTAAGTTTGCGGATTCAGACTTAATTGGAATCCCACTTCGTGTTACCATTGGTA
AGAAAGCTTCTGAAGGCATTGTTGAAGTCAAGTTACGTAAGACAGGGGAAGCAGTCGAAGTTAAGGTTGCGGAACTAAAT
AACACGGTAGAAATTTTATTAAACCAAGCTCAATAA

Upstream 100 bases:

>100_bases
AATTGGCTTTGGATTTTTAATGATATTAATGCTGTTAGTTACTTGGAATGATATTCAAAGATACTTTTTTTAAATTAACT
TAAGGAGTTCAAATTCAATT

Downstream 100 bases:

>100_bases
AAGTAAATAAACCAATTAAAGCTGGGGAATCCCAGCTTTAATTATTTGTAGCAAATATTTTTAATGGATTAGTAACATCA
GGGGGACCAAAGAGTGAGTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MKQSKILISTVKEVPNDAEVLSHKMMLRAGYIRQISAGMYAYLPLAYKVISKIEKIVREEMEAIDAVEMLTPAVLPAELW
KQSGRYETYGQELYKFKNRHDRDFILGPTHEETMTTLIRDEVKSYKKLPLSLYQIQMKYRDEDRPRYGLLRGREFLMKDA
YSFHADEETLDQSFRDFEKAYQNIFRRCGLNFREIVGDAGAMGGRDSKEFSAIASIGEDTIAYSEESDYAANLEMASSVY
TDLQMHENQEELTKVATDDAHSIDEVAAKLDVDSNRLIKTMVLIVDEAPVLALLRGNDQLNEVKLTNLLHADEVREATEE
EAFELLGAHVGSLGPVLENKPENLKIVADKYIEQMVNSVVGANEDGFHLKNVNVERDFTVDEYADIRTVREGELAPDGKG
ALKFTKGIEIGHIFKIGTKYSEALDAKVLDENGRAIPVIMGCYGIGVSRLLSAVSEQQSDENGLVWPKNIAPYDVHVIPV
NAKNAEQMEIADQINGELTKAGYDVLVDDRKERAGVKFADSDLIGIPLRVTIGKKASEGIVEVKLRKTGEAVEVKVAELN
NTVEILLNQAQ

Sequences:

>Translated_571_residues
MKQSKILISTVKEVPNDAEVLSHKMMLRAGYIRQISAGMYAYLPLAYKVISKIEKIVREEMEAIDAVEMLTPAVLPAELW
KQSGRYETYGQELYKFKNRHDRDFILGPTHEETMTTLIRDEVKSYKKLPLSLYQIQMKYRDEDRPRYGLLRGREFLMKDA
YSFHADEETLDQSFRDFEKAYQNIFRRCGLNFREIVGDAGAMGGRDSKEFSAIASIGEDTIAYSEESDYAANLEMASSVY
TDLQMHENQEELTKVATDDAHSIDEVAAKLDVDSNRLIKTMVLIVDEAPVLALLRGNDQLNEVKLTNLLHADEVREATEE
EAFELLGAHVGSLGPVLENKPENLKIVADKYIEQMVNSVVGANEDGFHLKNVNVERDFTVDEYADIRTVREGELAPDGKG
ALKFTKGIEIGHIFKIGTKYSEALDAKVLDENGRAIPVIMGCYGIGVSRLLSAVSEQQSDENGLVWPKNIAPYDVHVIPV
NAKNAEQMEIADQINGELTKAGYDVLVDDRKERAGVKFADSDLIGIPLRVTIGKKASEGIVEVKLRKTGEAVEVKVAELN
NTVEILLNQAQ
>Mature_571_residues
MKQSKILISTVKEVPNDAEVLSHKMMLRAGYIRQISAGMYAYLPLAYKVISKIEKIVREEMEAIDAVEMLTPAVLPAELW
KQSGRYETYGQELYKFKNRHDRDFILGPTHEETMTTLIRDEVKSYKKLPLSLYQIQMKYRDEDRPRYGLLRGREFLMKDA
YSFHADEETLDQSFRDFEKAYQNIFRRCGLNFREIVGDAGAMGGRDSKEFSAIASIGEDTIAYSEESDYAANLEMASSVY
TDLQMHENQEELTKVATDDAHSIDEVAAKLDVDSNRLIKTMVLIVDEAPVLALLRGNDQLNEVKLTNLLHADEVREATEE
EAFELLGAHVGSLGPVLENKPENLKIVADKYIEQMVNSVVGANEDGFHLKNVNVERDFTVDEYADIRTVREGELAPDGKG
ALKFTKGIEIGHIFKIGTKYSEALDAKVLDENGRAIPVIMGCYGIGVSRLLSAVSEQQSDENGLVWPKNIAPYDVHVIPV
NAKNAEQMEIADQINGELTKAGYDVLVDDRKERAGVKFADSDLIGIPLRVTIGKKASEGIVEVKLRKTGEAVEVKVAELN
NTVEILLNQAQ

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=38.7234042553192, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1786392, Length=576, Percent_Identity=46.5277777777778, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI115532348, Length=216, Percent_Identity=37.037037037037, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=547, Percent_Identity=29.2504570383912, Blast_Score=229, Evalue=6e-61,
Organism=Drosophila melanogaster, GI24656200, Length=215, Percent_Identity=33.4883720930233, Blast_Score=152, Evalue=6e-37,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PEDPA (Q03FS9)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804385.1
- ProteinModelPortal:   Q03FS9
- SMR:   Q03FS9
- STRING:   Q03FS9
- GeneID:   4417844
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0884
- NMPDR:   fig|278197.10.peg.786
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q03FS9
- BioCyc:   PPEN278197:PEPE_0884-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 64007; Mature: 64007

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSKILISTVKEVPNDAEVLSHKMMLRAGYIRQISAGMYAYLPLAYKVISKIEKIVREE
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MEAIDAVEMLTPAVLPAELWKQSGRYETYGQELYKFKNRHDRDFILGPTHEETMTTLIRD
HHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EVKSYKKLPLSLYQIQMKYRDEDRPRYGLLRGREFLMKDAYSFHADEETLDQSFRDFEKA
HHHHHHCCCHHEEHEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YQNIFRRCGLNFREIVGDAGAMGGRDSKEFSAIASIGEDTIAYSEESDYAANLEMASSVY
HHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
TDLQMHENQEELTKVATDDAHSIDEVAAKLDVDSNRLIKTMVLIVDEAPVLALLRGNDQL
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCEEEEEECCCCC
NEVKLTNLLHADEVREATEEEAFELLGAHVGSLGPVLENKPENLKIVADKYIEQMVNSVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEHHHHHHHHHHHHHH
GANEDGFHLKNVNVERDFTVDEYADIRTVREGELAPDGKGALKFTKGIEIGHIFKIGTKY
CCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCHH
SEALDAKVLDENGRAIPVIMGCYGIGVSRLLSAVSEQQSDENGLVWPKNIAPYDVHVIPV
HHHHCHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEE
NAKNAEQMEIADQINGELTKAGYDVLVDDRKERAGVKFADSDLIGIPLRVTIGKKASEGI
CCCCCHHHHHHHHHCCCEECCCCCEEECCCHHHCCCEECCCCEEEEEEEEEECCCCCCCE
VEVKLRKTGEAVEVKVAELNNTVEILLNQAQ
EEEEEECCCCEEEEEEHHCCCEEEHHEECCC
>Mature Secondary Structure
MKQSKILISTVKEVPNDAEVLSHKMMLRAGYIRQISAGMYAYLPLAYKVISKIEKIVREE
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
MEAIDAVEMLTPAVLPAELWKQSGRYETYGQELYKFKNRHDRDFILGPTHEETMTTLIRD
HHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
EVKSYKKLPLSLYQIQMKYRDEDRPRYGLLRGREFLMKDAYSFHADEETLDQSFRDFEKA
HHHHHHCCCHHEEHEEEHHCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
YQNIFRRCGLNFREIVGDAGAMGGRDSKEFSAIASIGEDTIAYSEESDYAANLEMASSVY
HHHHHHHCCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHH
TDLQMHENQEELTKVATDDAHSIDEVAAKLDVDSNRLIKTMVLIVDEAPVLALLRGNDQL
HHHHHCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCEEEEEECCCCC
NEVKLTNLLHADEVREATEEEAFELLGAHVGSLGPVLENKPENLKIVADKYIEQMVNSVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCEEEHHHHHHHHHHHHHH
GANEDGFHLKNVNVERDFTVDEYADIRTVREGELAPDGKGALKFTKGIEIGHIFKIGTKY
CCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCEEEEEEECCHH
SEALDAKVLDENGRAIPVIMGCYGIGVSRLLSAVSEQQSDENGLVWPKNIAPYDVHVIPV
HHHHCHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCEEEEEEE
NAKNAEQMEIADQINGELTKAGYDVLVDDRKERAGVKFADSDLIGIPLRVTIGKKASEGI
CCCCCHHHHHHHHHCCCEECCCCCEEECCCHHHCCCEECCCCEEEEEEEEEECCCCCCCE
VEVKLRKTGEAVEVKVAELNNTVEILLNQAQ
EEEEEECCCCEEEEEEHHCCCEEEHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA