Click here to switch to the map view.

The map label for this gene is fabI [H]

Identifier: 116492631

GI number: 116492631

Start: 867490

End: 868248

Strand: Direct

Name: fabI [H]

Synonym: PEPE_0865

Alternate gene names: 116492631

Gene position: 867490-868248 (Clockwise)

Preceding gene: 116492630

Following gene: 116492632

Centisome position: 47.34

GC content: 37.81

Gene sequence:

>759_bases
TTGGAAGGAATTCTTAGTGGAAAAACCATTGTCATTATGGGTGTAGCGAATAAGAACAGTATTGCTTGGGGTTGTACAAA
AGCAATCATGGATCAAGGCGCAAAAGTTGTATTAACTTACCAAAATGACCGGATTAAAAAAAGTTTAAATAAAATTGTGG
ACGATTCAATCGCATTGGTTGAATGTGATGTTTCAGAAGATGCCAATATTGAAAAGGCCTTTAAACAAATTGAAGATAAA
TTTGGTAAGGTTGATGGTATTATTCATGCGATTGCCTTTGCTGACAAGGAAACTCTTGAAGGTGGAGTTTTAAATACCAA
AAAAGAAGGTTACAATCTAGCTCAAGATGTTAGTGCTTACTCATTGATTGCTGTTTCACGCTATGGTGCGGAAATCATGA
ACGAAAAGGGTAGTATTGTGACGCTTACATACTTCGGCTCAACTCGTGCAATTCCTAACTACAACATGATGGGAATTGCT
AAAGCTTCACTTGAATCCAGCGTACGCTACTTGGCAAGTGATTTAGGTGACCGTCATATTCGAGTTAATGCTATTTCAGC
TGGTGCAATTAAGACACTTGCTGTTACAGGTATCAAGCATCATCGTGACTTGCTTAAAGAATCTCAAAGCCGTACAGTCG
ATGGTGAAAATGTAAGTACTAGTGAAGTTGGAAATGTCGCTGCATTCCTTGTTAGTGATATGGCAACTGGTGTAACCGGT
GATGTTATTTACGTTGATAAAGGCGTACATTTAATTTAA

Upstream 100 bases:

>100_bases
TTAGTTACAGAAGTAAATCGTTTAAGTCAATTTTCAGCTGATGAACTCGTGCAGCGACGACATGAACGATTTAGTAAATT
CTAGAAGAGGGGTTATGCAG

Downstream 100 bases:

>100_bases
GCTAAATAAAAAGATTAAGGTGGTTACCTTAATCTTTTTTTGTATCCTCAAATTGTCAGATATAGATAGATTTTTTGAGA
AATTGACTAGATGTAATTGC

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241 [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MEGILSGKTIVIMGVANKNSIAWGCTKAIMDQGAKVVLTYQNDRIKKSLNKIVDDSIALVECDVSEDANIEKAFKQIEDK
FGKVDGIIHAIAFADKETLEGGVLNTKKEGYNLAQDVSAYSLIAVSRYGAEIMNEKGSIVTLTYFGSTRAIPNYNMMGIA
KASLESSVRYLASDLGDRHIRVNAISAGAIKTLAVTGIKHHRDLLKESQSRTVDGENVSTSEVGNVAAFLVSDMATGVTG
DVIYVDKGVHLI

Sequences:

>Translated_252_residues
MEGILSGKTIVIMGVANKNSIAWGCTKAIMDQGAKVVLTYQNDRIKKSLNKIVDDSIALVECDVSEDANIEKAFKQIEDK
FGKVDGIIHAIAFADKETLEGGVLNTKKEGYNLAQDVSAYSLIAVSRYGAEIMNEKGSIVTLTYFGSTRAIPNYNMMGIA
KASLESSVRYLASDLGDRHIRVNAISAGAIKTLAVTGIKHHRDLLKESQSRTVDGENVSTSEVGNVAAFLVSDMATGVTG
DVIYVDKGVHLI
>Mature_252_residues
MEGILSGKTIVIMGVANKNSIAWGCTKAIMDQGAKVVLTYQNDRIKKSLNKIVDDSIALVECDVSEDANIEKAFKQIEDK
FGKVDGIIHAIAFADKETLEGGVLNTKKEGYNLAQDVSAYSLIAVSRYGAEIMNEKGSIVTLTYFGSTRAIPNYNMMGIA
KASLESSVRYLASDLGDRHIRVNAISAGAIKTLAVTGIKHHRDLLKESQSRTVDGENVSTSEVGNVAAFLVSDMATGVTG
DVIYVDKGVHLI

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787545, Length=252, Percent_Identity=43.2539682539683, Blast_Score=228, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24644339, Length=255, Percent_Identity=22.7450980392157, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: =1.3.1.9 [H]

Molecular weight: Translated: 27062; Mature: 27062

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGILSGKTIVIMGVANKNSIAWGCTKAIMDQGAKVVLTYQNDRIKKSLNKIVDDSIALV
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEE
ECDVSEDANIEKAFKQIEDKFGKVDGIIHAIAFADKETLEGGVLNTKKEGYNLAQDVSAY
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHH
SLIAVSRYGAEIMNEKGSIVTLTYFGSTRAIPNYNMMGIAKASLESSVRYLASDLGDRHI
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
RVNAISAGAIKTLAVTGIKHHRDLLKESQSRTVDGENVSTSEVGNVAAFLVSDMATGVTG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DVIYVDKGVHLI
CEEEEECCCCCC
>Mature Secondary Structure
MEGILSGKTIVIMGVANKNSIAWGCTKAIMDQGAKVVLTYQNDRIKKSLNKIVDDSIALV
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCEEEE
ECDVSEDANIEKAFKQIEDKFGKVDGIIHAIAFADKETLEGGVLNTKKEGYNLAQDVSAY
EECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCHHHHHHHH
SLIAVSRYGAEIMNEKGSIVTLTYFGSTRAIPNYNMMGIAKASLESSVRYLASDLGDRHI
HHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCEE
RVNAISAGAIKTLAVTGIKHHRDLLKESQSRTVDGENVSTSEVGNVAAFLVSDMATGVTG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
DVIYVDKGVHLI
CEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659 [H]