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The map label for this gene is ispA [C]

Identifier: 116492587

GI number: 116492587

Start: 825674

End: 826564

Strand: Direct

Name: ispA [C]

Synonym: PEPE_0820

Alternate gene names: 116492587

Gene position: 825674-826564 (Clockwise)

Preceding gene: 116492586

Following gene: 116492588

Centisome position: 45.06

GC content: 37.82

Gene sequence:

>891_bases
ATGAATAGTAATTTTGATGACTTTGTCAAACAAAACGTAGAATTACTTGATCAGCACATCGATGAATTGTTTGATAGTGA
TTCAACATTGAATACCGATTTAAAAATGATGATGAAGTATTCAATTGACGCTGGTGGCAAGCGAGTTCGTCCGTTAGTTT
TATTGGCAATTTTGAAAGCATTCGGAAAGACCCCTAATTACGATATCCTAACAGTCGCAGCAGCTCTCGAAGCTGTACAT
ACATACTCGTTGATTCATGATGATTTACCCGCAATGGATAACGATGATTTACGGCGTGGACGACCAACCAGCCATAAGAA
GTTTGGTGAGGCAGAGGCTATCTTGGCTGGTGATGCACTTTTAACGGTGGCCTTTCAATGGATTAGCTCAACCAATTTAA
AGGATCGGATTAAAAGTGAATTGGTGTTATCCTTGGCAAGTGATGCTGGGGCTTCAGGGATGATTGCTGGCCAATTATTG
GATATTCAAGGAAATCAGCGACATTATTCCTTAGCTGAACTCAAACATTTACATCATCTTAAAACCGGCAAAATGCTATT
ATTCCCAGCGGAAGCGGCGTTGATAATGAAGCCAAGCAAACTTCAAGTTGCCGAATTGGTTAAAGATTTTATGGCAAACT
TTGGTTTAGCCTTTCAAATCCATGACGATATAATTGATGTAACAGAAAGTACAGCGATCCTTGGTAAAACGGCTGGAAAA
GATGTAGATTTAAATAAAAATACGTACGTTAACTTACTCGGGTTAAGTGGAGCAAAAGAAGAATTAAATCAGGTTCTTCA
AAAATGTGAACAAGACTTAACCTCCTTAGGTGAATTACAGCCAGAAATGGATTTGATGATTTTAGAAGGTTTTTTAACGT
ATTTAAAATAA

Upstream 100 bases:

>100_bases
GAATTACAAGAAACCCTAAAAAATGCTGAAAAAACTCTTACAAAAGTTATGCAAGACAATGGTGAAGAGACTAACTTAGA
TTTGGGGACAGACGGAGAAA

Downstream 100 bases:

>100_bases
TGGAGAATTAACGATGGCAAAAGAACGCGTAGATGTATTGCTGGTAGAACAGGGACTTTTTGATACCCGTGAAAAAGCAA
AAAGAGCCGTAATGGCCGGA

Product: farnesyl-diphosphate synthase

Products: NA

Alternate protein names: FPP synthase; (2E,6E)-farnesyl diphosphate synthase; Geranyltranstransferase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MNSNFDDFVKQNVELLDQHIDELFDSDSTLNTDLKMMMKYSIDAGGKRVRPLVLLAILKAFGKTPNYDILTVAAALEAVH
TYSLIHDDLPAMDNDDLRRGRPTSHKKFGEAEAILAGDALLTVAFQWISSTNLKDRIKSELVLSLASDAGASGMIAGQLL
DIQGNQRHYSLAELKHLHHLKTGKMLLFPAEAALIMKPSKLQVAELVKDFMANFGLAFQIHDDIIDVTESTAILGKTAGK
DVDLNKNTYVNLLGLSGAKEELNQVLQKCEQDLTSLGELQPEMDLMILEGFLTYLK

Sequences:

>Translated_296_residues
MNSNFDDFVKQNVELLDQHIDELFDSDSTLNTDLKMMMKYSIDAGGKRVRPLVLLAILKAFGKTPNYDILTVAAALEAVH
TYSLIHDDLPAMDNDDLRRGRPTSHKKFGEAEAILAGDALLTVAFQWISSTNLKDRIKSELVLSLASDAGASGMIAGQLL
DIQGNQRHYSLAELKHLHHLKTGKMLLFPAEAALIMKPSKLQVAELVKDFMANFGLAFQIHDDIIDVTESTAILGKTAGK
DVDLNKNTYVNLLGLSGAKEELNQVLQKCEQDLTSLGELQPEMDLMILEGFLTYLK
>Mature_296_residues
MNSNFDDFVKQNVELLDQHIDELFDSDSTLNTDLKMMMKYSIDAGGKRVRPLVLLAILKAFGKTPNYDILTVAAALEAVH
TYSLIHDDLPAMDNDDLRRGRPTSHKKFGEAEAILAGDALLTVAFQWISSTNLKDRIKSELVLSLASDAGASGMIAGQLL
DIQGNQRHYSLAELKHLHHLKTGKMLLFPAEAALIMKPSKLQVAELVKDFMANFGLAFQIHDDIIDVTESTAILGKTAGK
DVDLNKNTYVNLLGLSGAKEELNQVLQKCEQDLTSLGELQPEMDLMILEGFLTYLK

Specific function: Unknown

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=273, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1786623, Length=291, Percent_Identity=41.2371134020619, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1789578, Length=270, Percent_Identity=28.1481481481481, Blast_Score=104, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17505681, Length=296, Percent_Identity=25, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24651612, Length=276, Percent_Identity=26.8115942028986, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.10 [H]

Molecular weight: Translated: 32715; Mature: 32715

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSNFDDFVKQNVELLDQHIDELFDSDSTLNTDLKMMMKYSIDAGGKRVRPLVLLAILKA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
FGKTPNYDILTVAAALEAVHTYSLIHDDLPAMDNDDLRRGRPTSHKKFGEAEAILAGDAL
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHCCCCHHHHCCHHH
LTVAFQWISSTNLKDRIKSELVLSLASDAGASGMIAGQLLDIQGNQRHYSLAELKHLHHL
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
KTGKMLLFPAEAALIMKPSKLQVAELVKDFMANFGLAFQIHDDIIDVTESTAILGKTAGK
CCCCEEEEECCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCC
DVDLNKNTYVNLLGLSGAKEELNQVLQKCEQDLTSLGELQPEMDLMILEGFLTYLK
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
>Mature Secondary Structure
MNSNFDDFVKQNVELLDQHIDELFDSDSTLNTDLKMMMKYSIDAGGKRVRPLVLLAILKA
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
FGKTPNYDILTVAAALEAVHTYSLIHDDLPAMDNDDLRRGRPTSHKKFGEAEAILAGDAL
HCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHCCCCHHHHCCHHH
LTVAFQWISSTNLKDRIKSELVLSLASDAGASGMIAGQLLDIQGNQRHYSLAELKHLHHL
HHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHH
KTGKMLLFPAEAALIMKPSKLQVAELVKDFMANFGLAFQIHDDIIDVTESTAILGKTAGK
CCCCEEEEECCEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCCC
DVDLNKNTYVNLLGLSGAKEELNQVLQKCEQDLTSLGELQPEMDLMILEGFLTYLK
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8486607; 7918490 [H]