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The map label for this gene is glnA [H]

Identifier: 116492507

GI number: 116492507

Start: 775762

End: 777102

Strand: Direct

Name: glnA [H]

Synonym: PEPE_0739

Alternate gene names: 116492507

Gene position: 775762-777102 (Clockwise)

Preceding gene: 116492506

Following gene: 116492508

Centisome position: 42.34

GC content: 38.4

Gene sequence:

>1341_bases
ATGGCAAAGAAAAATTATTCGCAAGCAGATATTCGTCAGATGGCAAAGGATGAAAATGTACGTTTTCTCCGATTAATGTT
TACAGATCTTTTTGGAATAATTAAGAACGTTGAAGTACCAATTAGTCAATTGGACAAACTATTAGATAATAAATTGATGT
TTGATGGTTCCTCAATTGACGGGTTTGTTCGGATTGAAGAAAGTGACATGTATTTATACCCAGATCTTTCTACTTGGATG
GTTTTCCCATGGGGAAGCGAACATGGCAAGGTGGCTCGCATTATTTGTGAAGTATACTCAAATGATCGTAAACCATTCGT
GGGTGATCCACGTAACAATTTAATTCGAGTACTCCAAGAGATGAAGGATGCAGGATTTACTGATTTTAATATCGGACCTG
AACCTGAGTTTTTCTTGTTGAAATTAGATGAAAATGGTAAACCAACCACTAATTTAAATGATAAAGGTAGTTACTTTGAT
TTAGCTCCTGTTGATTTAGGTGAAAACTGCCGTCGTGATATTGTTTTGGAACTTGAAAATATGGGCTTTGATGTTGAAGC
TTCTCATCATGAAGTTGCTCCAGGACAACACGAAATTGACTTTAAATACGCCGATGCTTTGACCGCTGCCGATAACATTC
AAACCTTTAAGTTGGTTGTTAAGACAGTTGCCCGTAAATATAACCTGCATGCTACATTTATGCCTAAACCTATGGATGGA
ATCAATGGTTCAGGGATGCATTTAAACATGTCACTTTTCAATAAGGAAGGCAATGCTTTCTATGACGAAAAGGGTGACTT
ACAACTTTCTCAAAATGCTTACTGGTTCCTTGGTGGACTATTGAAGCATGCTCGTAGTTATACGGCCGTATGTAACCCAA
TTGTTAACTCGTACAAACGTTTAGTTCCTGGATATGAAGCTCCAGTATACGTTGCTTGGTCAGGTTCAAACCGTTCACCA
CTTATTCGCGTTCCTTCAAGTAAGGGACTCTCAACTCGTTTTGAAGTTCGAAGCGTCGATCCAGCTGCTAACCCATACTT
AGCAATTGCATCAGTATTGGAAGCAGGCTTAGATGGCATTAGAAACAAGATTGAACCAGAAGATTCCGTTGATCGTAATA
TCTATCGAATGAACATTCAAGAACGTAATGAAGAGCATATTACAGATCTACCTTCAACATTACACAATGCTTTGAAGGAA
TTCCAAAATGATGATGTAATGCGTAAGGCATTAGGGGATCACATTTTCCAAAGCTTCCTCGAAGCTAAGAAGTTAGAATG
GGCTTCTTACCGTCAAGAAGTGACACAATGGGAACGTGATCAATATCTCGAAATGTTCTAG

Upstream 100 bases:

>100_bases
GAATTACTTCAAACTGGAAGGCTATCGGATGATTCTGGCCCAGCCTTTCCAATTAAACGAGTTTAGTATAAAAAAATAAA
GTGGGGCGTTACATACAGTT

Downstream 100 bases:

>100_bases
ACAAATATCTATAATAAGAGTTATAACACGGTTAATCGTGTTGTAACTCTTTTTTTGTGAAGAATGTTTAAATTTATTGT
CGAATTCCTTTATTTATTTG

Product: L-glutamine synthetase

Products: NA

Alternate protein names: GS; Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MAKKNYSQADIRQMAKDENVRFLRLMFTDLFGIIKNVEVPISQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWM
VFPWGSEHGKVARIICEVYSNDRKPFVGDPRNNLIRVLQEMKDAGFTDFNIGPEPEFFLLKLDENGKPTTNLNDKGSYFD
LAPVDLGENCRRDIVLELENMGFDVEASHHEVAPGQHEIDFKYADALTAADNIQTFKLVVKTVARKYNLHATFMPKPMDG
INGSGMHLNMSLFNKEGNAFYDEKGDLQLSQNAYWFLGGLLKHARSYTAVCNPIVNSYKRLVPGYEAPVYVAWSGSNRSP
LIRVPSSKGLSTRFEVRSVDPAANPYLAIASVLEAGLDGIRNKIEPEDSVDRNIYRMNIQERNEEHITDLPSTLHNALKE
FQNDDVMRKALGDHIFQSFLEAKKLEWASYRQEVTQWERDQYLEMF

Sequences:

>Translated_446_residues
MAKKNYSQADIRQMAKDENVRFLRLMFTDLFGIIKNVEVPISQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWM
VFPWGSEHGKVARIICEVYSNDRKPFVGDPRNNLIRVLQEMKDAGFTDFNIGPEPEFFLLKLDENGKPTTNLNDKGSYFD
LAPVDLGENCRRDIVLELENMGFDVEASHHEVAPGQHEIDFKYADALTAADNIQTFKLVVKTVARKYNLHATFMPKPMDG
INGSGMHLNMSLFNKEGNAFYDEKGDLQLSQNAYWFLGGLLKHARSYTAVCNPIVNSYKRLVPGYEAPVYVAWSGSNRSP
LIRVPSSKGLSTRFEVRSVDPAANPYLAIASVLEAGLDGIRNKIEPEDSVDRNIYRMNIQERNEEHITDLPSTLHNALKE
FQNDDVMRKALGDHIFQSFLEAKKLEWASYRQEVTQWERDQYLEMF
>Mature_445_residues
AKKNYSQADIRQMAKDENVRFLRLMFTDLFGIIKNVEVPISQLDKLLDNKLMFDGSSIDGFVRIEESDMYLYPDLSTWMV
FPWGSEHGKVARIICEVYSNDRKPFVGDPRNNLIRVLQEMKDAGFTDFNIGPEPEFFLLKLDENGKPTTNLNDKGSYFDL
APVDLGENCRRDIVLELENMGFDVEASHHEVAPGQHEIDFKYADALTAADNIQTFKLVVKTVARKYNLHATFMPKPMDGI
NGSGMHLNMSLFNKEGNAFYDEKGDLQLSQNAYWFLGGLLKHARSYTAVCNPIVNSYKRLVPGYEAPVYVAWSGSNRSPL
IRVPSSKGLSTRFEVRSVDPAANPYLAIASVLEAGLDGIRNKIEPEDSVDRNIYRMNIQERNEEHITDLPSTLHNALKEF
QNDDVMRKALGDHIFQSFLEAKKLEWASYRQEVTQWERDQYLEMF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=464, Percent_Identity=28.0172413793103, Blast_Score=150, Evalue=2e-36,
Organism=Escherichia coli, GI1790301, Length=437, Percent_Identity=40.045766590389, Blast_Score=282, Evalue=3e-77,
Organism=Escherichia coli, GI87081870, Length=457, Percent_Identity=27.3522975929978, Blast_Score=150, Evalue=1e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51043; Mature: 50912

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKNYSQADIRQMAKDENVRFLRLMFTDLFGIIKNVEVPISQLDKLLDNKLMFDGSSID
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLSTWMVFPWGSEHGKVARIICEVYSNDRKPFVGDPRNNLIRVLQE
EEEEEECCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MKDAGFTDFNIGPEPEFFLLKLDENGKPTTNLNDKGSYFDLAPVDLGENCRRDIVLELEN
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECC
MGFDVEASHHEVAPGQHEIDFKYADALTAADNIQTFKLVVKTVARKYNLHATFMPKPMDG
CCCCEECCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCC
INGSGMHLNMSLFNKEGNAFYDEKGDLQLSQNAYWFLGGLLKHARSYTAVCNPIVNSYKR
CCCCCEEEEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGYEAPVYVAWSGSNRSPLIRVPSSKGLSTRFEVRSVDPAANPYLAIASVLEAGLDGI
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RNKIEPEDSVDRNIYRMNIQERNEEHITDLPSTLHNALKEFQNDDVMRKALGDHIFQSFL
HHCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EAKKLEWASYRQEVTQWERDQYLEMF
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKKNYSQADIRQMAKDENVRFLRLMFTDLFGIIKNVEVPISQLDKLLDNKLMFDGSSID
CCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCC
GFVRIEESDMYLYPDLSTWMVFPWGSEHGKVARIICEVYSNDRKPFVGDPRNNLIRVLQE
EEEEEECCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
MKDAGFTDFNIGPEPEFFLLKLDENGKPTTNLNDKGSYFDLAPVDLGENCRRDIVLELEN
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEEEECC
MGFDVEASHHEVAPGQHEIDFKYADALTAADNIQTFKLVVKTVARKYNLHATFMPKPMDG
CCCCEECCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHHHHHHHCCEEEECCCCCCC
INGSGMHLNMSLFNKEGNAFYDEKGDLQLSQNAYWFLGGLLKHARSYTAVCNPIVNSYKR
CCCCCEEEEEEEEECCCCEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPGYEAPVYVAWSGSNRSPLIRVPSSKGLSTRFEVRSVDPAANPYLAIASVLEAGLDGI
HCCCCCCCEEEEECCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RNKIEPEDSVDRNIYRMNIQERNEEHITDLPSTLHNALKEFQNDDVMRKALGDHIFQSFL
HHCCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
EAKKLEWASYRQEVTQWERDQYLEMF
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA