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The map label for this gene is pbpF [H]

Identifier: 116492440

GI number: 116492440

Start: 710987

End: 713140

Strand: Direct

Name: pbpF [H]

Synonym: PEPE_0672

Alternate gene names: 116492440

Gene position: 710987-713140 (Clockwise)

Preceding gene: 116492439

Following gene: 116492441

Centisome position: 38.8

GC content: 38.86

Gene sequence:

>2154_bases
ATGAATATGAAAAAAATAGGGAGTGCGATTAAAGCATTCTTACAAAAGATTGGTGCATTTTTAAAACCCGTGTGGGATAA
AATTCACCCGTTCATCCATAAGTACTGGCACAGATATCAGCTTACGCGATGGATTATTGTAGTAGTTTTAGCAGTTGTAT
TTTTCTCAAGTGCGATTTTAACTTATCAAGCGAAGACTGCTAATGTAAAAAATTTAAAAGCAGCTTTATCCCAGCCTACT
TTAGTTTATGATAAAAATAATGCTAAAGCTGGAAGTTTATATTCTCAAAAAGGAACCTGGACAAATCTAGATAATATCTC
ACCTAACGTTCAAAATGCCGTAATTGCGACGGAAGACCGAAACTTTTATAAGGAATATGGTTTTTCAATTAAGGGAATCG
GTAGAGCGTTTGTGATGTACGGGGTCAATAAATTACTTGGTCGAGATTATATATCTGGTGGGGGAAGTACTTTAACCCAA
CAATTAGTAAAAAATGCCTTTCTATCACAGCAACAGACCTTTACTAGAAAGGCTAAAGAGTTATTTTTATCAATTGAAGT
TGAAAATGTTTATTCAAAAAAAGATATCTTAGCAATGTACTTGAATAACGCCTATTTTGGAAATGGGGTTTGGGGAGTAC
AGGATGCTGCTGAGAGGTATTTCGGGGTCAGTGCTTCGGAACTATCGGTTCCCCAAGCGGCAACCTTGGCAGGGATGCTG
ACTAATCCGAGTGGCTATAATCCGATCGATCATCCAAAAAATGCCTTTTCACGCCGTAATGTAGTTTTAAGTTTGATGGC
GGAAACCGGTAAGATATCCAAGGCCGACGCCAAATCCTATGCTCAAACAGCTATCTCAACCAGTGATAATTACAATAGTG
AGGACGGTTATAAGTATCCATACTACTTTGATGCAGTAATCGATGAAGCCATTAGCGAGTATGGATTGACGGAGTCGGAT
ATTATGAATCGCGGGTATAAAATTTATACGTATCTGGATCAGGATAGTCAGCAAGGTATGCAAGATACTTTTGACAACTC
CGCTAATTTTCCGGTTGATGCTACAGATGGTACTAAAGTACAGGCTGCATCTGTTGCAGTAAATGCCAAAAATGGTGGTG
TAGAAGCGGTAGTTGGTGGTCGTGGTAATCATGTCTTTAGAGGCTATAACCGAGCTACCCAGATTAAACGCCAACCTGGC
TCAACCTTAAAACCTATTGCAGTTTACGCCCCAGCTTTAGCGAGTGGATATAGTTATAGTTCTATGTTAGAAGATAAACT
TCAAACATATGGAACTAATAAATATAAACCTGAAAATTACAATAACGAATATGCAGGAAAGATTGCGATGTACCAAGCAC
TTGAACAAAGTAAAAATGCGCCTGCGGTATGGTTATTAAATAAAATTGGTGTAGATAAGGGTTACGATTACACCAAGAAA
TTCGGCTTAAACCCAAGCAAAGATGATAAGAACCTAGCGATGGCTCTTGGTGGATTGACGAATGGTGTATCGCCAATTCA
ACTAGCTTCTGCTTATACTGCATTTGCTAATAATGGAACGTTGTCGAAGACTGGATTTATTCGCAAAATTGTAGATGCTT
CTGGAAATACGATCGTAAATAACACCACGGAATCCAAACGAGTTATTTCAAAAAAGGTCGCTAAAGAAATGACCAGTATG
ATGTTAGGTGTTTATGGTGACAGTTCTGGCACAGGTTATGGGGCAGAACCTTTTGGTTATACAATTGCTGGTAAAACAGG
TAGTACTGAAGCAGATACAGGAACTAGTCCATCAGATGCAACCCGTGACAAATGGATGGTAGGATATACAAAGGATGTTG
TAGTTGCTACTTGGGAAGGGTTTGATGACACAAACAAGACCCATCATCTGGAAAATATTACGGGAGTCGGCATGAATAGT
ATGTTCAAGAGTGAAATGAGTGCCATTTTACCAAATACGAAAGGTACTTCATTTGACGTTAAATCAGCTTCTACTCTAGC
TAATAGTGGAAACAATGTTACATCAAACGTTTGGGACAATATTGAAGACGGAACTTCTTCCATCACAGATAGTATTTCTC
GAGGAGCTAGTGGGGTCAAAGAGAAAGCGGGAGAATTCTTCAACGAAGCTAAAAAGTTCATTACCGGGAATTGA

Upstream 100 bases:

>100_bases
TAGGTTATTAAGACGAAGTTAAAAAGGGCAATCAACCTCGGTAGAATAACATTGATATGATAAACTATTAAAGTATCGTA
ATCAGCAACGAGGTAAATGT

Downstream 100 bases:

>100_bases
TTTTTATATTAAAAACAAGGCCTAAATGATATAATTAAGATGTTAATTTCAGAGGAGGCGTACTATGGCTGTAAACATTT
ATGATACTGCAAACCAAATG

Product: membrane carboxypeptidase (penicillin-binding protein)

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MNMKKIGSAIKAFLQKIGAFLKPVWDKIHPFIHKYWHRYQLTRWIIVVVLAVVFFSSAILTYQAKTANVKNLKAALSQPT
LVYDKNNAKAGSLYSQKGTWTNLDNISPNVQNAVIATEDRNFYKEYGFSIKGIGRAFVMYGVNKLLGRDYISGGGSTLTQ
QLVKNAFLSQQQTFTRKAKELFLSIEVENVYSKKDILAMYLNNAYFGNGVWGVQDAAERYFGVSASELSVPQAATLAGML
TNPSGYNPIDHPKNAFSRRNVVLSLMAETGKISKADAKSYAQTAISTSDNYNSEDGYKYPYYFDAVIDEAISEYGLTESD
IMNRGYKIYTYLDQDSQQGMQDTFDNSANFPVDATDGTKVQAASVAVNAKNGGVEAVVGGRGNHVFRGYNRATQIKRQPG
STLKPIAVYAPALASGYSYSSMLEDKLQTYGTNKYKPENYNNEYAGKIAMYQALEQSKNAPAVWLLNKIGVDKGYDYTKK
FGLNPSKDDKNLAMALGGLTNGVSPIQLASAYTAFANNGTLSKTGFIRKIVDASGNTIVNNTTESKRVISKKVAKEMTSM
MLGVYGDSSGTGYGAEPFGYTIAGKTGSTEADTGTSPSDATRDKWMVGYTKDVVVATWEGFDDTNKTHHLENITGVGMNS
MFKSEMSAILPNTKGTSFDVKSASTLANSGNNVTSNVWDNIEDGTSSITDSISRGASGVKEKAGEFFNEAKKFITGN

Sequences:

>Translated_717_residues
MNMKKIGSAIKAFLQKIGAFLKPVWDKIHPFIHKYWHRYQLTRWIIVVVLAVVFFSSAILTYQAKTANVKNLKAALSQPT
LVYDKNNAKAGSLYSQKGTWTNLDNISPNVQNAVIATEDRNFYKEYGFSIKGIGRAFVMYGVNKLLGRDYISGGGSTLTQ
QLVKNAFLSQQQTFTRKAKELFLSIEVENVYSKKDILAMYLNNAYFGNGVWGVQDAAERYFGVSASELSVPQAATLAGML
TNPSGYNPIDHPKNAFSRRNVVLSLMAETGKISKADAKSYAQTAISTSDNYNSEDGYKYPYYFDAVIDEAISEYGLTESD
IMNRGYKIYTYLDQDSQQGMQDTFDNSANFPVDATDGTKVQAASVAVNAKNGGVEAVVGGRGNHVFRGYNRATQIKRQPG
STLKPIAVYAPALASGYSYSSMLEDKLQTYGTNKYKPENYNNEYAGKIAMYQALEQSKNAPAVWLLNKIGVDKGYDYTKK
FGLNPSKDDKNLAMALGGLTNGVSPIQLASAYTAFANNGTLSKTGFIRKIVDASGNTIVNNTTESKRVISKKVAKEMTSM
MLGVYGDSSGTGYGAEPFGYTIAGKTGSTEADTGTSPSDATRDKWMVGYTKDVVVATWEGFDDTNKTHHLENITGVGMNS
MFKSEMSAILPNTKGTSFDVKSASTLANSGNNVTSNVWDNIEDGTSSITDSISRGASGVKEKAGEFFNEAKKFITGN
>Mature_717_residues
MNMKKIGSAIKAFLQKIGAFLKPVWDKIHPFIHKYWHRYQLTRWIIVVVLAVVFFSSAILTYQAKTANVKNLKAALSQPT
LVYDKNNAKAGSLYSQKGTWTNLDNISPNVQNAVIATEDRNFYKEYGFSIKGIGRAFVMYGVNKLLGRDYISGGGSTLTQ
QLVKNAFLSQQQTFTRKAKELFLSIEVENVYSKKDILAMYLNNAYFGNGVWGVQDAAERYFGVSASELSVPQAATLAGML
TNPSGYNPIDHPKNAFSRRNVVLSLMAETGKISKADAKSYAQTAISTSDNYNSEDGYKYPYYFDAVIDEAISEYGLTESD
IMNRGYKIYTYLDQDSQQGMQDTFDNSANFPVDATDGTKVQAASVAVNAKNGGVEAVVGGRGNHVFRGYNRATQIKRQPG
STLKPIAVYAPALASGYSYSSMLEDKLQTYGTNKYKPENYNNEYAGKIAMYQALEQSKNAPAVWLLNKIGVDKGYDYTKK
FGLNPSKDDKNLAMALGGLTNGVSPIQLASAYTAFANNGTLSKTGFIRKIVDASGNTIVNNTTESKRVISKKVAKEMTSM
MLGVYGDSSGTGYGAEPFGYTIAGKTGSTEADTGTSPSDATRDKWMVGYTKDVVVATWEGFDDTNKTHHLENITGVGMNS
MFKSEMSAILPNTKGTSFDVKSASTLANSGNNVTSNVWDNIEDGTSSITDSISRGASGVKEKAGEFFNEAKKFITGN

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=533, Percent_Identity=30.9568480300188, Blast_Score=190, Evalue=3e-49,
Organism=Escherichia coli, GI87082258, Length=309, Percent_Identity=33.0097087378641, Blast_Score=156, Evalue=4e-39,
Organism=Escherichia coli, GI1788867, Length=545, Percent_Identity=28.6238532110092, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1789601, Length=177, Percent_Identity=38.9830508474576, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 78241; Mature: 78241

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMKKIGSAIKAFLQKIGAFLKPVWDKIHPFIHKYWHRYQLTRWIIVVVLAVVFFSSAIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYQAKTANVKNLKAALSQPTLVYDKNNAKAGSLYSQKGTWTNLDNISPNVQNAVIATEDR
EEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
NFYKEYGFSIKGIGRAFVMYGVNKLLGRDYISGGGSTLTQQLVKNAFLSQQQTFTRKAKE
HHHHHHCCCEEHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFLSIEVENVYSKKDILAMYLNNAYFGNGVWGVQDAAERYFGVSASELSVPQAATLAGML
HEEEEEHHHHHCHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHEE
TNPSGYNPIDHPKNAFSRRNVVLSLMAETGKISKADAKSYAQTAISTSDNYNSEDGYKYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECC
YYFDAVIDEAISEYGLTESDIMNRGYKIYTYLDQDSQQGMQDTFDNSANFPVDATDGTKV
EEHHHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCHHCCHHHHCCCCCCCCEECCCCCEE
QAASVAVNAKNGGVEAVVGGRGNHVFRGYNRATQIKRQPGSTLKPIAVYAPALASGYSYS
EEEEEEEECCCCCEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCHHHCCHHHHCCCCHH
SMLEDKLQTYGTNKYKPENYNNEYAGKIAMYQALEQSKNAPAVWLLNKIGVDKGYDYTKK
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCHHHH
FGLNPSKDDKNLAMALGGLTNGVSPIQLASAYTAFANNGTLSKTGFIRKIVDASGNTIVN
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEC
NTTESKRVISKKVAKEMTSMMLGVYGDSSGTGYGAEPFGYTIAGKTGSTEADTGTSPSDA
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TRDKWMVGYTKDVVVATWEGFDDTNKTHHLENITGVGMNSMFKSEMSAILPNTKGTSFDV
CCCCEEEEECCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEC
KSASTLANSGNNVTSNVWDNIEDGTSSITDSISRGASGVKEKAGEFFNEAKKFITGN
CHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNMKKIGSAIKAFLQKIGAFLKPVWDKIHPFIHKYWHRYQLTRWIIVVVLAVVFFSSAIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYQAKTANVKNLKAALSQPTLVYDKNNAKAGSLYSQKGTWTNLDNISPNVQNAVIATEDR
EEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCC
NFYKEYGFSIKGIGRAFVMYGVNKLLGRDYISGGGSTLTQQLVKNAFLSQQQTFTRKAKE
HHHHHHCCCEEHHHHHHHHHHHHHHHCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LFLSIEVENVYSKKDILAMYLNNAYFGNGVWGVQDAAERYFGVSASELSVPQAATLAGML
HEEEEEHHHHHCHHHHHHHHHCCCEECCCCCCHHHHHHHHCCCCHHHCCCCHHHHHHHEE
TNPSGYNPIDHPKNAFSRRNVVLSLMAETGKISKADAKSYAQTAISTSDNYNSEDGYKYP
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCEECC
YYFDAVIDEAISEYGLTESDIMNRGYKIYTYLDQDSQQGMQDTFDNSANFPVDATDGTKV
EEHHHHHHHHHHHHCCCHHHHHHCCEEEEEEECCCHHCCHHHHCCCCCCCCEECCCCCEE
QAASVAVNAKNGGVEAVVGGRGNHVFRGYNRATQIKRQPGSTLKPIAVYAPALASGYSYS
EEEEEEEECCCCCEEEEECCCCCCEEECCCHHHHHHCCCCCCCCCHHHCCHHHHCCCCHH
SMLEDKLQTYGTNKYKPENYNNEYAGKIAMYQALEQSKNAPAVWLLNKIGVDKGYDYTKK
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHCCCCCCCHHHH
FGLNPSKDDKNLAMALGGLTNGVSPIQLASAYTAFANNGTLSKTGFIRKIVDASGNTIVN
CCCCCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEC
NTTESKRVISKKVAKEMTSMMLGVYGDSSGTGYGAEPFGYTIAGKTGSTEADTGTSPSDA
CCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCC
TRDKWMVGYTKDVVVATWEGFDDTNKTHHLENITGVGMNSMFKSEMSAILPNTKGTSFDV
CCCCEEEEECCCEEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCEEC
KSASTLANSGNNVTSNVWDNIEDGTSSITDSISRGASGVKEKAGEFFNEAKKFITGN
CHHHHHHCCCCCCCHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]