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The map label for this gene is ytzG [H]

Identifier: 116492417

GI number: 116492417

Start: 690173

End: 690892

Strand: Direct

Name: ytzG [H]

Synonym: PEPE_0647

Alternate gene names: 116492417

Gene position: 690173-690892 (Clockwise)

Preceding gene: 116492416

Following gene: 116492419

Centisome position: 37.67

GC content: 38.89

Gene sequence:

>720_bases
ATGCGAGTAGATAAATTTTTACATGACATGAATATGGGAACGCGAACCCAAATTCGAAAAATGATTAAAGACAAGCTAAT
CTTTGTTAACGATACGGTCGTTAAAACTGGGCGAGTTAAAGTGGATCCAGTGGTTGATGAGGTTAGATTAAAAGACCAAG
TGATTGAGTATCAACAATATTTCTATTTCATGATGAACAAACCATTGGGAGTCTTAACTGCTACGGAAGATCGTAAACAA
AAAACCGTTATGGATTTATTCAACAAGGTCGATCTCCGAAATGATTTATTTCCAGTCGGAAGATTGGATAAAGATACCCA
AGGCTTACTGCTAATCACTAATAACGGGCAGTTAGCGCATGATTTATTAGCACCAGAGCGGCACGTTGATAAAACTTACG
AGGCGGTGGTGACTGGAAAAATCACCGATCAAGCTGTCGAAGAATTTCAAAAAGGAATCACCTTAAGGGATGGTACTACG
TTAAAACCAGCGCAACTGATCATTGAACACTATTCAGAATTAGATGATCAAACAGTGGTTAAGGTAGTCCTAAAAGAAGG
AAAGTATCATCAGGTTCGCCGAATGTTTGGTAGCCTAGGTGAGAAAGTGGTCACTCTTCGAAGGACTTCCTTTGGTCCTT
TAGAATTAGATGTGGGATTACTTCCCGGAACTTATCGAGAATTAACGGATGATGAAATTAATCGGTTAGTTGAAGGTTAA

Upstream 100 bases:

>100_bases
TTTATCTCTACTTTGTATTAAAGAGCCGCCTAGCGGATGAAATTGTGGGGGCACGGGCAGCTGGCTTACGTCGAATGCTA
AAGATTAGTTAGGAGTAGAC

Downstream 100 bases:

>100_bases
AAATAAGCTACTATGACAAAAATCATAGTAGCTTATTTTTTTGAAGTTGTTTTAGTGTGAAGGGGAGACGACGAATTAAG
CGATGGGGCAAAACAACCGC

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MRVDKFLHDMNMGTRTQIRKMIKDKLIFVNDTVVKTGRVKVDPVVDEVRLKDQVIEYQQYFYFMMNKPLGVLTATEDRKQ
KTVMDLFNKVDLRNDLFPVGRLDKDTQGLLLITNNGQLAHDLLAPERHVDKTYEAVVTGKITDQAVEEFQKGITLRDGTT
LKPAQLIIEHYSELDDQTVVKVVLKEGKYHQVRRMFGSLGEKVVTLRRTSFGPLELDVGLLPGTYRELTDDEINRLVEG

Sequences:

>Translated_239_residues
MRVDKFLHDMNMGTRTQIRKMIKDKLIFVNDTVVKTGRVKVDPVVDEVRLKDQVIEYQQYFYFMMNKPLGVLTATEDRKQ
KTVMDLFNKVDLRNDLFPVGRLDKDTQGLLLITNNGQLAHDLLAPERHVDKTYEAVVTGKITDQAVEEFQKGITLRDGTT
LKPAQLIIEHYSELDDQTVVKVVLKEGKYHQVRRMFGSLGEKVVTLRRTSFGPLELDVGLLPGTYRELTDDEINRLVEG
>Mature_239_residues
MRVDKFLHDMNMGTRTQIRKMIKDKLIFVNDTVVKTGRVKVDPVVDEVRLKDQVIEYQQYFYFMMNKPLGVLTATEDRKQ
KTVMDLFNKVDLRNDLFPVGRLDKDTQGLLLITNNGQLAHDLLAPERHVDKTYEAVVTGKITDQAVEEFQKGITLRDGTT
LKPAQLIIEHYSELDDQTVVKVVLKEGKYHQVRRMFGSLGEKVVTLRRTSFGPLELDVGLLPGTYRELTDDEINRLVEG

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=34.8739495798319, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1790453, Length=236, Percent_Identity=27.9661016949153, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1787524, Length=246, Percent_Identity=30.8943089430894, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=187, Percent_Identity=33.6898395721925, Blast_Score=89, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27481; Mature: 27481

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDKFLHDMNMGTRTQIRKMIKDKLIFVNDTVVKTGRVKVDPVVDEVRLKDQVIEYQQY
CCHHHHHHHCCCCHHHHHHHHHHHCEEEEECCEEECCCEEECCHHHHHHHHHHHHHHHHH
FYFMMNKPLGVLTATEDRKQKTVMDLFNKVDLRNDLFPVGRLDKDTQGLLLITNNGQLAH
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHH
DLLAPERHVDKTYEAVVTGKITDQAVEEFQKGITLRDGTTLKPAQLIIEHYSELDDQTVV
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCHHHHH
KVVLKEGKYHQVRRMFGSLGEKVVTLRRTSFGPLELDVGLLPGTYRELTDDEINRLVEG
HHHHHCCCHHHHHHHHHHHHHHEEHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHCC
>Mature Secondary Structure
MRVDKFLHDMNMGTRTQIRKMIKDKLIFVNDTVVKTGRVKVDPVVDEVRLKDQVIEYQQY
CCHHHHHHHCCCCHHHHHHHHHHHCEEEEECCEEECCCEEECCHHHHHHHHHHHHHHHHH
FYFMMNKPLGVLTATEDRKQKTVMDLFNKVDLRNDLFPVGRLDKDTQGLLLITNNGQLAH
HHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCHH
DLLAPERHVDKTYEAVVTGKITDQAVEEFQKGITLRDGTTLKPAQLIIEHYSELDDQTVV
HHHCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCHHHHH
KVVLKEGKYHQVRRMFGSLGEKVVTLRRTSFGPLELDVGLLPGTYRELTDDEINRLVEG
HHHHHCCCHHHHHHHHHHHHHHEEHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]