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The map label for this gene is guaC

Identifier: 116492389

GI number: 116492389

Start: 649596

End: 650573

Strand: Direct

Name: guaC

Synonym: PEPE_0595

Alternate gene names: 116492389

Gene position: 649596-650573 (Clockwise)

Preceding gene: 116492388

Following gene: 116492391

Centisome position: 35.45

GC content: 40.49

Gene sequence:

>978_bases
ATGAAAGTATTTGACTATGAAGACATCCAATTAATTCCAGCAAAATGTATCGTCCGTAGTAGAACCGAATGTGATCCTAC
AGTGGTCTTGGGAGAACATCGTTTTAAACTACCCGTGGTTCCAGCTAATATGCAAACTATCATCAATGAAGAGATTGCTG
AAAAGTTAGCTGAGGATGGATACTTCTACATTATGCATCGTTTTGAACCTGAAAGAAGAATGGACTTTGTTAAAAAGATG
AAAGACAAAGGCTTGATCAGTTCAATTAGTGTTGGGGTTAAAGACGATGAATATGCACTAATTGATCAACTTGCTGAAGA
AAATCTTACTCCTGATTACATTACCATCGACGTGGCTCATGGCCATGCTCAAAGTGTAATCGACATGATCCACTACATTA
AAGAAAAATTACCAGCTGCTTTCGTGATTGCAGGAAACGTTGGAACGCAAGAAGGAGTCCGTGAACTGGAAAATGCTGGT
GCCGATGCAACTAAAGTCGGAATCGGACCTGGTAAAGTATGTATTACTAAGATTAAGACCGGCTTTGGAACTGGTGGTTG
GCAATTATCAGCTCTCCGTTGGTGTGCTAAAGTGGCTCGTAAGCCATTAATCGCTGATGGAGGAATTCGGACTCATGGTG
ATATTGCTAAATCAATTCGATTTGGTGCAACTATGGTGATGATTGGTTCATTATTTGCCGGACATATCGAATCACCAGGT
GAAACTAAGGTGGAAGATGGCGTGAAGTATAAAGAATACTTCGGTAGTGCTTCGCAATACCAAAAGGGTGAAGCTAAAAA
CGTTGAAGGTAAGAAGATTTGGATCCATCAACGTGGACATTTACGTGATACACTCCAAGCAATGCGTGAAGATCTCCAAT
CATCGATTTCCTATGCGGGCGGTAGAGATTTAGAAGCCATCCGTAAGGTCGATTATGTGATTGTGAAGAATTCGATTTTT
AATGGTGATGTATTGTAA

Upstream 100 bases:

>100_bases
AGAAAACGAACTTTAAATGCTTGTTTTATATTTAAAGTTAGTGTATATTATTTCGTGGTGACAAAAGTACACCAAATTTA
TGCACTTCGAGGGGACAAAA

Downstream 100 bases:

>100_bases
AAAAGAGTGTGAGCGAAACTTGGCTGGTTTCGCTCATACATCTCCAACACCAAAATCTAAAAAAATAACTCAACTTAAGC
GCAAATCTGAACTAAAGGTT

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MKVFDYEDIQLIPAKCIVRSRTECDPTVVLGEHRFKLPVVPANMQTIINEEIAEKLAEDGYFYIMHRFEPERRMDFVKKM
KDKGLISSISVGVKDDEYALIDQLAEENLTPDYITIDVAHGHAQSVIDMIHYIKEKLPAAFVIAGNVGTQEGVRELENAG
ADATKVGIGPGKVCITKIKTGFGTGGWQLSALRWCAKVARKPLIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHIESPG
ETKVEDGVKYKEYFGSASQYQKGEAKNVEGKKIWIHQRGHLRDTLQAMREDLQSSISYAGGRDLEAIRKVDYVIVKNSIF
NGDVL

Sequences:

>Translated_325_residues
MKVFDYEDIQLIPAKCIVRSRTECDPTVVLGEHRFKLPVVPANMQTIINEEIAEKLAEDGYFYIMHRFEPERRMDFVKKM
KDKGLISSISVGVKDDEYALIDQLAEENLTPDYITIDVAHGHAQSVIDMIHYIKEKLPAAFVIAGNVGTQEGVRELENAG
ADATKVGIGPGKVCITKIKTGFGTGGWQLSALRWCAKVARKPLIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHIESPG
ETKVEDGVKYKEYFGSASQYQKGEAKNVEGKKIWIHQRGHLRDTLQAMREDLQSSISYAGGRDLEAIRKVDYVIVKNSIF
NGDVL
>Mature_325_residues
MKVFDYEDIQLIPAKCIVRSRTECDPTVVLGEHRFKLPVVPANMQTIINEEIAEKLAEDGYFYIMHRFEPERRMDFVKKM
KDKGLISSISVGVKDDEYALIDQLAEENLTPDYITIDVAHGHAQSVIDMIHYIKEKLPAAFVIAGNVGTQEGVRELENAG
ADATKVGIGPGKVCITKIKTGFGTGGWQLSALRWCAKVARKPLIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHIESPG
ETKVEDGVKYKEYFGSASQYQKGEAKNVEGKKIWIHQRGHLRDTLQAMREDLQSSISYAGGRDLEAIRKVDYVIVKNSIF
NGDVL

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI50541954, Length=338, Percent_Identity=32.8402366863905, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541952, Length=338, Percent_Identity=32.8402366863905, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541948, Length=338, Percent_Identity=32.8402366863905, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI50541956, Length=338, Percent_Identity=32.8402366863905, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI156104880, Length=338, Percent_Identity=29.8816568047337, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI217035150, Length=332, Percent_Identity=31.9277108433735, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI217035146, Length=332, Percent_Identity=31.9277108433735, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI217035148, Length=332, Percent_Identity=31.9277108433735, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI34328930, Length=332, Percent_Identity=31.9277108433735, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI156616279, Length=332, Percent_Identity=31.9277108433735, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI217035152, Length=332, Percent_Identity=31.9277108433735, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI34328928, Length=332, Percent_Identity=31.9277108433735, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI66933016, Length=325, Percent_Identity=29.8461538461538, Blast_Score=125, Evalue=4e-29,
Organism=Escherichia coli, GI1786293, Length=337, Percent_Identity=30.5637982195846, Blast_Score=150, Evalue=8e-38,
Organism=Escherichia coli, GI1788855, Length=220, Percent_Identity=36.8181818181818, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560440, Length=323, Percent_Identity=33.1269349845201, Blast_Score=159, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71994385, Length=310, Percent_Identity=31.6129032258064, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71994389, Length=236, Percent_Identity=34.7457627118644, Blast_Score=100, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6323464, Length=184, Percent_Identity=34.7826086956522, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322012, Length=184, Percent_Identity=34.2391304347826, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6323585, Length=184, Percent_Identity=34.2391304347826, Blast_Score=103, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319352, Length=138, Percent_Identity=32.6086956521739, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641071, Length=200, Percent_Identity=37, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24641073, Length=200, Percent_Identity=37, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI28571163, Length=200, Percent_Identity=37, Blast_Score=118, Evalue=6e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_PEDPA (Q03GJ0)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804124.1
- ProteinModelPortal:   Q03GJ0
- SMR:   Q03GJ0
- STRING:   Q03GJ0
- GeneID:   4418216
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0595
- NMPDR:   fig|278197.10.peg.547
- eggNOG:   COG0516
- HOGENOM:   HBG298985
- OMA:   PDYITID
- PhylomeDB:   Q03GJ0
- ProtClustDB:   PRK05458
- BioCyc:   PPEN278197:PEPE_0595-MONOMER
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 36062; Mature: 36062

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVFDYEDIQLIPAKCIVRSRTECDPTVVLGEHRFKLPVVPANMQTIINEEIAEKLAEDG
CCCCCCCCCEEEHHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCC
YFYIMHRFEPERRMDFVKKMKDKGLISSISVGVKDDEYALIDQLAEENLTPDYITIDVAH
EEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
GHAQSVIDMIHYIKEKLPAAFVIAGNVGTQEGVRELENAGADATKVGIGPGKVCITKIKT
CHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEECC
GFGTGGWQLSALRWCAKVARKPLIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHIESPG
CCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ETKVEDGVKYKEYFGSASQYQKGEAKNVEGKKIWIHQRGHLRDTLQAMREDLQSSISYAG
CCCHHCCCHHHHHCCCHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
GRDLEAIRKVDYVIVKNSIFNGDVL
CCCHHHHHHHHEEEEECCCCCCCCC
>Mature Secondary Structure
MKVFDYEDIQLIPAKCIVRSRTECDPTVVLGEHRFKLPVVPANMQTIINEEIAEKLAEDG
CCCCCCCCCEEEHHHHHHHCCCCCCCEEEECCCEEEEEECCCHHHHHHHHHHHHHHHCCC
YFYIMHRFEPERRMDFVKKMKDKGLISSISVGVKDDEYALIDQLAEENLTPDYITIDVAH
EEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEECC
GHAQSVIDMIHYIKEKLPAAFVIAGNVGTQEGVRELENAGADATKVGIGPGKVCITKIKT
CHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEECC
GFGTGGWQLSALRWCAKVARKPLIADGGIRTHGDIAKSIRFGATMVMIGSLFAGHIESPG
CCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ETKVEDGVKYKEYFGSASQYQKGEAKNVEGKKIWIHQRGHLRDTLQAMREDLQSSISYAG
CCCHHCCCHHHHHCCCHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCC
GRDLEAIRKVDYVIVKNSIFNGDVL
CCCHHHHHHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA