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The map label for this gene is scrB

Identifier: 116492317

GI number: 116492317

Start: 574186

End: 575691

Strand: Direct

Name: scrB

Synonym: PEPE_0519

Alternate gene names: 116492317

Gene position: 574186-575691 (Clockwise)

Preceding gene: 116492313

Following gene: 116492318

Centisome position: 31.34

GC content: 49.0

Gene sequence:

>1506_bases
ATGATATGGAATCGTAAAACCCGTTATACCCCTTATGAACAGTGGCCAGCAACTAAGCTACCACAGCTCGTTGCCCAGGC
ACGCCAGTCTAAGTGGCGGATGCAACATCATATTCAGCCTACATCAGGTTTGTTAAATGACCCCAATGGCTTTTCATACT
TTGATGGTCAGTGGCACCTGTTCTACCAAGTCTTTCCGTTCGGTCCGGTACACGGACTGAAGTCATGGCAACACGTGACC
TCTAAGAACTTGGTGGATTGGCATGATGAGGGCTTAGCGATTCGACCAGATACGCCGTACGACTCACACGGCGCGTACAC
GGGTACAGCACTACCAATTGATGATCAGTTATTTATTATGTATACGGGTAATGTCCGTACTGCCGACTGGCAACGTGAAT
CGTATCAATTAGGTGCCTGGATGGACACGGATAATCACATTAAAAAGCTGTCCCGGCCACTAATTGCGCATGCTCCGGCT
GGGTACACGAGTTCGTTTCGTGATCCGGACTTGATCCGGAATGACCACGGCTACTACGCGCTGATCGGTGCGCAGACGAC
TACTGAGATCGGTGCTATCTTGGTTTATTTCTCAAAGGATCTGACCACCTGGACGTGTCAAGGCGAGTTGAACGTTCCCG
CAAATGCGCGGGGTTATATGATTGAGTGTCCAAATCTGGTCTGGATCGATCAACAGCCCGTCTTATTGTTCTGTCCCCAA
GGGTTATCGCAAGCAACGATCCCGTATCAGAATATTTATCCGAACATGTACTTAGTTGCCGATCAATTGAACCTAGCTCA
GGCACAATTCACTGAACCCCACGCCCTGACGCAATTGGATGACGGCTTTGACGTGTATGCCACGCAGGCAATCAATGCGC
CGGATGGCCGCGCGTTAGCCGTCAGCTGGATTGGGTTACCTGAAATTTCCTATCCAACTGATCGTGAAAATTGGGCCCAT
TGTTTGAGCTTAGTTAAGGAACTAACGCTTAAAGATGGGCACCTCTATCAAAATCCAGTGGCAGCGGTGGATGATTTGCG
GACGACGGCGCATGACCTAGTCTTTGAGCAACAACGGGCGACGGTGGCCGCTTTGAATGGCAGCTTTGAACTCTTACTGA
CGGTTCCCGCTGATAAAACGGTCACGGTTAACATTGCTGATCAGCAGGAAAGTGGCCAGCTGCAGGTGACTGTTGATGCC
AACCATGGTCAGGTTATGATTGATCGGCGGCATACGGGGAATTCGTTTGCTGAAGATTATGGTCAGACTCGTCAAGTTGA
GTTAACGGCCCATAAGACGATAAAAATCCGGCTAATCATAGATGTGTCAGTCTTTGAATGCTATATTGATAACGGCTATT
CCGTGATGACCGGCCGTTTCTTCTTAAACGCTACCCCGAGTCGTCTTAACGTTCAAGGTGACACTACGGCAGTTACCGGT
AAAGTCTGGGAATGGCGCCAATCAGAGCATACTGGAGTCGATAACAATGAAACCAAAATTAAATGA

Upstream 100 bases:

>100_bases
ATGAAAATATAATATAAAAATGTCAAACGATTGACATATCGGTGTAAGCGGTTTTATAATAGTGACAATTAAGTCGAACG
TTAAAAAGGAGCTCATCGTC

Downstream 100 bases:

>100_bases
TGTTGCGAAGCTAGCGGGGGTCTCAGCGACAACCGTTTCTCGGGTTATTAATAACCATGGCTACCTGAGTAGTCAGACTA
AAGAAAAAGTTTTCGCGGCG

Product: beta-fructofuranosidase

Products: NA

Alternate protein names: Sucrase; Invertase

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQARQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRNDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQATIPYQNIYPNMYLVADQLNLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK

Sequences:

>Translated_501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQARQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRNDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQATIPYQNIYPNMYLVADQLNLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK
>Mature_501_residues
MIWNRKTRYTPYEQWPATKLPQLVAQARQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHLFYQVFPFGPVHGLKSWQHVT
SKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIMYTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPA
GYTSSFRDPDLIRNDHGYYALIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
GLSQATIPYQNIYPNMYLVADQLNLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALAVSWIGLPEISYPTDRENWAH
CLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRATVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDA
NHGQVMIDRRHTGNSFAEDYGQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
KVWEWRQSEHTGVDNNETKIK

Specific function: Unknown

COG id: COG1621

COG function: function code G; Beta-fructosidases (levanase/invertase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 32 family

Homologues:

Organism=Saccharomyces cerevisiae, GI6322029, Length=505, Percent_Identity=22.970297029703, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SCRB_PEDPE (P43471)

Other databases:

- EMBL:   Z32771
- EMBL:   L32093
- PIR:   S44258
- ProteinModelPortal:   P43471
- SMR:   P43471
- BRENDA:   3.2.1.26
- GO:   GO:0005737
- InterPro:   IPR008985
- InterPro:   IPR001362
- InterPro:   IPR018053
- InterPro:   IPR013189
- InterPro:   IPR013148
- InterPro:   IPR006232
- SMART:   SM00640
- TIGRFAMs:   TIGR01322

Pfam domain/function: PF08244 Glyco_hydro_32C; PF00251 Glyco_hydro_32N; SSF49899 ConA_like_lec_gl

EC number: =3.2.1.26

Molecular weight: Translated: 56690; Mature: 56690

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS00609 GLYCOSYL_HYDROL_F32

Important sites: ACT_SITE 47-47 BINDING 63-63 BINDING 222-222

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIWNRKTRYTPYEQWPATKLPQLVAQARQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHL
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEE
FYQVFPFGPVHGLKSWQHVTSKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIM
EEEEECCCCCHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCCEEEEE
YTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPAGYTSSFRDPDLIRNDHGYYA
EECCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCCCCCCCCCCCCCEECCCCEEE
LIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
EEECCCCCCCEEEEEEEECCCEEEEEECEECCCCCCCEEEEECCCEEEECCCCEEEECCC
GLSQATIPYQNIYPNMYLVADQLNLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALA
CCCCCCCCHHHCCCCEEEEEECCCHHHHHCCCCCHHEECCCCCEEEEEEEECCCCCCEEE
VSWIGLPEISYPTDRENWAHCLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCC
TVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDANHGQVMIDRRHTGNSFAEDY
EEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHC
GQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
CCCEEEEEEEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCEEEEC
KVWEWRQSEHTGVDNNETKIK
CEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIWNRKTRYTPYEQWPATKLPQLVAQARQSKWRMQHHIQPTSGLLNDPNGFSYFDGQWHL
CCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCEEE
FYQVFPFGPVHGLKSWQHVTSKNLVDWHDEGLAIRPDTPYDSHGAYTGTALPIDDQLFIM
EEEEECCCCCHHHHHHHHHHCCCCEEECCCCEEECCCCCCCCCCCCCCEEECCCCEEEEE
YTGNVRTADWQRESYQLGAWMDTDNHIKKLSRPLIAHAPAGYTSSFRDPDLIRNDHGYYA
EECCEEECCCCCCCEEEEEEECCHHHHHHHCCCEEEECCCCCCCCCCCCCCEECCCCEEE
LIGAQTTTEIGAILVYFSKDLTTWTCQGELNVPANARGYMIECPNLVWIDQQPVLLFCPQ
EEECCCCCCCEEEEEEEECCCEEEEEECEECCCCCCCEEEEECCCEEEECCCCEEEECCC
GLSQATIPYQNIYPNMYLVADQLNLAQAQFTEPHALTQLDDGFDVYATQAINAPDGRALA
CCCCCCCCHHHCCCCEEEEEECCCHHHHHCCCCCHHEECCCCCEEEEEEEECCCCCCEEE
VSWIGLPEISYPTDRENWAHCLSLVKELTLKDGHLYQNPVAAVDDLRTTAHDLVFEQQRA
EEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHCCC
TVAALNGSFELLLTVPADKTVTVNIADQQESGQLQVTVDANHGQVMIDRRHTGNSFAEDY
EEEEECCCEEEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCEEEEEECCCCCHHHHHC
GQTRQVELTAHKTIKIRLIIDVSVFECYIDNGYSVMTGRFFLNATPSRLNVQGDTTAVTG
CCCEEEEEEEEEEEEEEEEEEEEEEEEEECCCEEEEEEEEEEECCCCEEEECCCCEEEEC
KVWEWRQSEHTGVDNNETKIK
CEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA