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The map label for this gene is rafP [H]

Identifier: 116492311

GI number: 116492311

Start: 562111

End: 564039

Strand: Direct

Name: rafP [H]

Synonym: PEPE_0512

Alternate gene names: 116492311

Gene position: 562111-564039 (Clockwise)

Preceding gene: 116492309

Following gene: 116492312

Centisome position: 30.68

GC content: 40.02

Gene sequence:

>1929_bases
ATGCAGGAAGAACATAATTATAAAATGGGGTTAGGAGGTCGCCTAATCTATGGTTTTGGTGCTTTTGGTAATGATGCTTT
CTATAGTATCCTCTCCGGATATTTAATTATCTTCATTACTTCACACCTTTTTGATACCGGTAACAAAGCACTAGATAATC
GAATGGTCAGTTTGGTGACCTTGATTATTATGGTGTTACGAATCGTTGAGCTATTCATTGATCCCTTTATCGGTAACGCA
ATTGACCGAACTAAAACACGCTGGGGTCATTTCCGGCCATGGGTTGTAGTCGGTGGGACCGTCTCTTCAATTATTCTCTT
GCTATTATTTACTAATTTAGGCGGCCTCTATGCTAAAAACGCAATGATTTATTTGGTCGTTTTTGCAATTTTGTATATTA
CGATGGATATTTTCTATTCGTTTAAAGACGTTGGTTTTTGGTCAATGTTACCTTCCTTGACCACTGATTCACGAGAACGC
GAAAAAACTGCTACTTTTGCCCGCTTAGGGTCAACTATTGGTGGTGGCTTGGTTGGTGTGTTGGTCATGCCGGCAGTTAT
CTTCTTTTCTGCCAAGGCGACTAGTACTGGCGATAATCGTGGTTGGTTCATTTTTGCCTTGATCATCTGCTTGATAGCTT
TAATTTCAGCCTGGGGTGTTGGCTTAGGTACACGTGAAGTTGATAGTGATATTCGTAAAAATAAACAAGATACAGTTGGT
GTGATGGAGATTTTTAAGGCACTGGCTAAAAATGATCAGTTGCTATGGGCAGCTTTAGCCTATTTATTCTATGGTGTGGG
CATTAATATTTTAGGTTCACTGGAAGTCTATTATTTCACGTATATTATGGGTAAGCCGAAATCATTTTCTATTCTATCGA
TCATTAATATTTTCTTAGGTTTGATTGCTACATCGCTATTTCCAGTATTATCGAAAAAATTCAGTCGTAAAGGTGTTTTT
GCTGGCTGTTTAGTGTTCATGCTAGGTGGTATTGCGATTTTTACCATTGCTGGCAGTAATTTATGGCTGGTTCTATTAGC
GGCAACTATGTTTGGGTTCCCGCAACAGATGGTCTTTTTAGTAGTTTTGATGGTAATCACGGATTCTGTTGAATACGGTC
AATTGAAGCTGGGGCATCGTGATGAGTCGCTGGCTTTATCAGTGCGGCCGTTGATCGATAAATTTGGTGGCGCTATCTCT
AACGGGGTTGTTGGCCAAATTGCCATTATTTCTGGTATGACGACTGGTGCGACTGCTTCTTCTATCACTGCTGCAGGACA
ATTACATTTTAAATTAACGATGTTTGCCTTTCCCGCGTTAATGCTACTTATCGCTATTGGCATTTTTTCAAAGCAAATAT
TCTTAACCGAAGAAAAACACGCCGAAATTGTGGCTGAACTTGAAAGAACCTGGAGAACAAAGTTCGATAATACCACTGAT
CAGGTAGCAGAAAAAGTAGTTACATCACTTGATTTAGCTACACCAATCGCTGGGCAAGTGATTCCACTTGCCCAAGTCAA
TGATCCAACTTTTGCGGCTGGAACGTTAGGTGACGGATTTGCTATTAAACCTAGTGATGGCCGAATATTAGCTCCATTTG
ATGCAACGGTACGTCAAGTATTTACCACACGACATGCAGTTGGCTTAGTCGGTGATAATGGGATCGTCTTATTGATCCAT
ATTGGTTTGGGAACTGTTAAACTTAGAGGAACGGGATTTATTTCTTATGTTGAGGAGGGGCAGCATGTACAACAAGGGGA
TGAATTACTTGAGTTTTGGGATCCAACGATCAAACAAGCTGGTTTAGATGATACGGTTATTATGACAGTGACTAATTCAA
CCGAATTCACTATGATGGATTGGTTAGTCAAGCCAGGTCAAGCCGTTAAAGCAACTGATAATATTTTACAGTTGCATACT
AAAGCATAA

Upstream 100 bases:

>100_bases
GATAAAGATTACTATGGGCAACCCAATAGACAGGAATTATAATGAGCTTTGTTAATGAAAACGGATACAAAAAATGATTT
GAAGGGCAGGTAATTCATTT

Downstream 100 bases:

>100_bases
TGTGCCACTGGTTATCATTTAATTTTAAAATTAGTAATTCCCCGAGGAGGAAATAAAATGTCATTAATTACGGTTGATCA
AGCGAATCGCGTTTTTCATT

Product: Na+/xyloside symporter related transporter

Products: Li (I) [Cytoplasm]; melibiose [Cytoplasm]; Na (I) [Cytoplasm]; melibiose [Cytoplasm]; Proton [Cytoplasm]; melibiose [Cytoplasm] [C]

Alternate protein names: Raffinose permease; Putative phosphotransferase enzyme EIIA component; Putative PTS system EIIA component [H]

Number of amino acids: Translated: 642; Mature: 642

Protein sequence:

>642_residues
MQEEHNYKMGLGGRLIYGFGAFGNDAFYSILSGYLIIFITSHLFDTGNKALDNRMVSLVTLIIMVLRIVELFIDPFIGNA
IDRTKTRWGHFRPWVVVGGTVSSIILLLLFTNLGGLYAKNAMIYLVVFAILYITMDIFYSFKDVGFWSMLPSLTTDSRER
EKTATFARLGSTIGGGLVGVLVMPAVIFFSAKATSTGDNRGWFIFALIICLIALISAWGVGLGTREVDSDIRKNKQDTVG
VMEIFKALAKNDQLLWAALAYLFYGVGINILGSLEVYYFTYIMGKPKSFSILSIINIFLGLIATSLFPVLSKKFSRKGVF
AGCLVFMLGGIAIFTIAGSNLWLVLLAATMFGFPQQMVFLVVLMVITDSVEYGQLKLGHRDESLALSVRPLIDKFGGAIS
NGVVGQIAIISGMTTGATASSITAAGQLHFKLTMFAFPALMLLIAIGIFSKQIFLTEEKHAEIVAELERTWRTKFDNTTD
QVAEKVVTSLDLATPIAGQVIPLAQVNDPTFAAGTLGDGFAIKPSDGRILAPFDATVRQVFTTRHAVGLVGDNGIVLLIH
IGLGTVKLRGTGFISYVEEGQHVQQGDELLEFWDPTIKQAGLDDTVIMTVTNSTEFTMMDWLVKPGQAVKATDNILQLHT
KA

Sequences:

>Translated_642_residues
MQEEHNYKMGLGGRLIYGFGAFGNDAFYSILSGYLIIFITSHLFDTGNKALDNRMVSLVTLIIMVLRIVELFIDPFIGNA
IDRTKTRWGHFRPWVVVGGTVSSIILLLLFTNLGGLYAKNAMIYLVVFAILYITMDIFYSFKDVGFWSMLPSLTTDSRER
EKTATFARLGSTIGGGLVGVLVMPAVIFFSAKATSTGDNRGWFIFALIICLIALISAWGVGLGTREVDSDIRKNKQDTVG
VMEIFKALAKNDQLLWAALAYLFYGVGINILGSLEVYYFTYIMGKPKSFSILSIINIFLGLIATSLFPVLSKKFSRKGVF
AGCLVFMLGGIAIFTIAGSNLWLVLLAATMFGFPQQMVFLVVLMVITDSVEYGQLKLGHRDESLALSVRPLIDKFGGAIS
NGVVGQIAIISGMTTGATASSITAAGQLHFKLTMFAFPALMLLIAIGIFSKQIFLTEEKHAEIVAELERTWRTKFDNTTD
QVAEKVVTSLDLATPIAGQVIPLAQVNDPTFAAGTLGDGFAIKPSDGRILAPFDATVRQVFTTRHAVGLVGDNGIVLLIH
IGLGTVKLRGTGFISYVEEGQHVQQGDELLEFWDPTIKQAGLDDTVIMTVTNSTEFTMMDWLVKPGQAVKATDNILQLHT
KA
>Mature_642_residues
MQEEHNYKMGLGGRLIYGFGAFGNDAFYSILSGYLIIFITSHLFDTGNKALDNRMVSLVTLIIMVLRIVELFIDPFIGNA
IDRTKTRWGHFRPWVVVGGTVSSIILLLLFTNLGGLYAKNAMIYLVVFAILYITMDIFYSFKDVGFWSMLPSLTTDSRER
EKTATFARLGSTIGGGLVGVLVMPAVIFFSAKATSTGDNRGWFIFALIICLIALISAWGVGLGTREVDSDIRKNKQDTVG
VMEIFKALAKNDQLLWAALAYLFYGVGINILGSLEVYYFTYIMGKPKSFSILSIINIFLGLIATSLFPVLSKKFSRKGVF
AGCLVFMLGGIAIFTIAGSNLWLVLLAATMFGFPQQMVFLVVLMVITDSVEYGQLKLGHRDESLALSVRPLIDKFGGAIS
NGVVGQIAIISGMTTGATASSITAAGQLHFKLTMFAFPALMLLIAIGIFSKQIFLTEEKHAEIVAELERTWRTKFDNTTD
QVAEKVVTSLDLATPIAGQVIPLAQVNDPTFAAGTLGDGFAIKPSDGRILAPFDATVRQVFTTRHAVGLVGDNGIVLLIH
IGLGTVKLRGTGFISYVEEGQHVQQGDELLEFWDPTIKQAGLDDTVIMTVTNSTEFTMMDWLVKPGQAVKATDNILQLHT
KA

Specific function: Responsible For Melibiose Transport. It Is Capable Of Using Hydrogen, Sodium, And Lithium Cations As Coupling Cations For Cotransport, Depending On The Particular Sugar Transported (Symport System). [C]

COG id: COG2211

COG function: function code G; Na+/melibiose symporter and related transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1790561, Length=473, Percent_Identity=27.2727272727273, Blast_Score=154, Evalue=2e-38,
Organism=Escherichia coli, GI1790159, Length=149, Percent_Identity=37.5838926174497, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1788757, Length=131, Percent_Identity=33.587786259542, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI145693206, Length=484, Percent_Identity=23.1404958677686, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786894, Length=111, Percent_Identity=39.6396396396396, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI48994989, Length=495, Percent_Identity=23.2323232323232, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787902, Length=425, Percent_Identity=22.8235294117647, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87082306, Length=433, Percent_Identity=22.1709006928406, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1786466, Length=354, Percent_Identity=24.2937853107345, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001927
- InterPro:   IPR018043
- InterPro:   IPR001127 [H]

Pfam domain/function: PF07690 MFS_1; PF00358 PTS_EIIA_1 [H]

EC number: NA

Molecular weight: Translated: 70095; Mature: 70095

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS00639 THIOL_PROTEASE_HIS ; PS00872 NA_GALACTOSIDE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEEHNYKMGLGGRLIYGFGAFGNDAFYSILSGYLIIFITSHLFDTGNKALDNRMVSLVT
CCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LIIMVLRIVELFIDPFIGNAIDRTKTRWGHFRPWVVVGGTVSSIILLLLFTNLGGLYAKN
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHH
AMIYLVVFAILYITMDIFYSFKDVGFWSMLPSLTTDSREREKTATFARLGSTIGGGLVGV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
LVMPAVIFFSAKATSTGDNRGWFIFALIICLIALISAWGVGLGTREVDSDIRKNKQDTVG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
VMEIFKALAKNDQLLWAALAYLFYGVGINILGSLEVYYFTYIMGKPKSFSILSIINIFLG
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHH
LIATSLFPVLSKKFSRKGVFAGCLVFMLGGIAIFTIAGSNLWLVLLAATMFGFPQQMVFL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCHHHHHHH
VVLMVITDSVEYGQLKLGHRDESLALSVRPLIDKFGGAISNGVVGQIAIISGMTTGATAS
HHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHCCHHCCCCCEEEEEEECCCCCCCHH
SITAAGQLHFKLTMFAFPALMLLIAIGIFSKQIFLTEEKHAEIVAELERTWRTKFDNTTD
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHCCCCCHH
QVAEKVVTSLDLATPIAGQVIPLAQVNDPTFAAGTLGDGFAIKPSDGRILAPFDATVRQV
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCEEECCCCCCEECCHHHHHHHH
FTTRHAVGLVGDNGIVLLIHIGLGTVKLRGTGFISYVEEGQHVQQGDELLEFWDPTIKQA
HHHHHHEEEECCCCEEEEEEECCCEEEEECCCEEHHHHCCCHHHHHHHHHHHCCHHHHHC
GLDDTVIMTVTNSTEFTMMDWLVKPGQAVKATDNILQLHTKA
CCCCEEEEEEECCCCEEHHHHHHCCCCCEEHHHHHHEEECCC
>Mature Secondary Structure
MQEEHNYKMGLGGRLIYGFGAFGNDAFYSILSGYLIIFITSHLFDTGNKALDNRMVSLVT
CCCCCCCEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LIIMVLRIVELFIDPFIGNAIDRTKTRWGHFRPWVVVGGTVSSIILLLLFTNLGGLYAKN
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHCCCHHHHH
AMIYLVVFAILYITMDIFYSFKDVGFWSMLPSLTTDSREREKTATFARLGSTIGGGLVGV
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHCHHHHHH
LVMPAVIFFSAKATSTGDNRGWFIFALIICLIALISAWGVGLGTREVDSDIRKNKQDTVG
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHH
VMEIFKALAKNDQLLWAALAYLFYGVGINILGSLEVYYFTYIMGKPKSFSILSIINIFLG
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHH
LIATSLFPVLSKKFSRKGVFAGCLVFMLGGIAIFTIAGSNLWLVLLAATMFGFPQQMVFL
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCHHHHHHH
VVLMVITDSVEYGQLKLGHRDESLALSVRPLIDKFGGAISNGVVGQIAIISGMTTGATAS
HHHHHHHCCCCCCEEEECCCCCCCEEEHHHHHHHHCCHHCCCCCEEEEEEECCCCCCCHH
SITAAGQLHFKLTMFAFPALMLLIAIGIFSKQIFLTEEKHAEIVAELERTWRTKFDNTTD
HHHHCCEEEEEHHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHCCCCCHH
QVAEKVVTSLDLATPIAGQVIPLAQVNDPTFAAGTLGDGFAIKPSDGRILAPFDATVRQV
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCEEECCCCCCEECCHHHHHHHH
FTTRHAVGLVGDNGIVLLIHIGLGTVKLRGTGFISYVEEGQHVQQGDELLEFWDPTIKQA
HHHHHHEEEECCCCEEEEEEECCCEEEEECCCEEHHHHCCCHHHHHHHHHHHCCHHHHHC
GLDDTVIMTVTNSTEFTMMDWLVKPGQAVKATDNILQLHTKA
CCCCEEEEEEECCCCEEHHHHHHCCCCCEEHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Li (I) [Periplasm]; melibiose [Periplasm]; Na (I) [Periplasm]; melibiose [Periplasm]; Proton [Periplasm]; melibiose [Periplasm] [C]

Specific reaction: Li (I) [Periplasm] + melibiose [Periplasm] = Li (I) [Cytoplasm] + melibiose [Cytoplasm] Na (I) [Periplasm] + melibiose [Periplasm] = Na (I) [Cytoplasm] + melibiose [Cytoplasm] Proton [Periplasm] + melibiose [Periplasm] = Proton [Cytoplasm] + melibiose [Cy

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA