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The map label for this gene is rafR [H]

Identifier: 116492310

GI number: 116492310

Start: 561046

End: 561876

Strand: Reverse

Name: rafR [H]

Synonym: PEPE_0511

Alternate gene names: 116492310

Gene position: 561876-561046 (Counterclockwise)

Preceding gene: 116492314

Following gene: 116492308

Centisome position: 30.66

GC content: 38.39

Gene sequence:

>831_bases
ATGAATGGTGAATATAAAACTTTAGCGAATAAAAGCTTCGAAAGTAATGTACTCTTTTTCGGGCAAGAGGCTTGCCTACC
GAATTACACTTACAAAGGTAATAATGTCAGAGACAGCTATGTTATCCATTACATCCAGGAGGGTAAAGGTACCTTTGCTG
CAGCTAACCATCCAGCAACCGTATTAAAAGCCGGCGATATTTTTATACTACCTAAGGGAACACCTTGTTTCTATCAAGCT
GACAATGACCAACCGTGGAAATACTTTTGGATCGGTTTTTCCGGTATCCGTATCGAAGCAATGCTTTCTGGTTCTTTATT
AGCTCAGAAATGCTACTTACGCCAAGTACAAAATGGCCAATTTTACGCCGATCTCTCTGAACTTTATAAAGTTTTACACA
TCCCTAATTCATTGATAAATGATGTTCTATTAGGGTCTTTGATCTATCGAATGTTCTATGACTTATTGCGCTGGTATCCC
GCTGACGCCACCAACATAAAAGTAAAGTCAACTGAACAATTTAACTTAGCTGTTTCTTACTTGCAAGAAAATTATAGTAC
TGGTTGTACCATTATGGACCTCTGTCATTATCTTAATTTATCACGAAGCTATCTCTACACCTTGTTTAAAACGCACGCTA
ATACCTCGCCACAAAAATTACTAACTAAATTGCGCTTAGAAGATGCTAAACAGCGTCTAAGCACTAGTAATAATTCAGTG
CAAAGCATTGCCAATATGGTAGGCTACAAGGATAGCTTCACTTTCTCCAAGGCATTTAAACGCTACAGTGGTGCTAGCCC
CAGCTATTATCGTAAAAGCATTGGAATCTAA

Upstream 100 bases:

>100_bases
CATGTTTTTCTAAATAGCAGTATAGTGTAGCTATTTATTAATTAGTTGAAAAACATTTTCACATATTTAGCAAGATATTT
TAAGTAAATGGAGAACTCAT

Downstream 100 bases:

>100_bases
ATCATAATGACCACTATGTTGCCCGTGTGATTTGTCTTTCTGCTAATGCTATCGAATTACATAAGGAATAGTTGACCTGT
AAAAATTATGGTACCACCGT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNGEYKTLANKSFESNVLFFGQEACLPNYTYKGNNVRDSYVIHYIQEGKGTFAAANHPATVLKAGDIFILPKGTPCFYQA
DNDQPWKYFWIGFSGIRIEAMLSGSLLAQKCYLRQVQNGQFYADLSELYKVLHIPNSLINDVLLGSLIYRMFYDLLRWYP
ADATNIKVKSTEQFNLAVSYLQENYSTGCTIMDLCHYLNLSRSYLYTLFKTHANTSPQKLLTKLRLEDAKQRLSTSNNSV
QSIANMVGYKDSFTFSKAFKRYSGASPSYYRKSIGI

Sequences:

>Translated_276_residues
MNGEYKTLANKSFESNVLFFGQEACLPNYTYKGNNVRDSYVIHYIQEGKGTFAAANHPATVLKAGDIFILPKGTPCFYQA
DNDQPWKYFWIGFSGIRIEAMLSGSLLAQKCYLRQVQNGQFYADLSELYKVLHIPNSLINDVLLGSLIYRMFYDLLRWYP
ADATNIKVKSTEQFNLAVSYLQENYSTGCTIMDLCHYLNLSRSYLYTLFKTHANTSPQKLLTKLRLEDAKQRLSTSNNSV
QSIANMVGYKDSFTFSKAFKRYSGASPSYYRKSIGI
>Mature_276_residues
MNGEYKTLANKSFESNVLFFGQEACLPNYTYKGNNVRDSYVIHYIQEGKGTFAAANHPATVLKAGDIFILPKGTPCFYQA
DNDQPWKYFWIGFSGIRIEAMLSGSLLAQKCYLRQVQNGQFYADLSELYKVLHIPNSLINDVLLGSLIYRMFYDLLRWYP
ADATNIKVKSTEQFNLAVSYLQENYSTGCTIMDLCHYLNLSRSYLYTLFKTHANTSPQKLLTKLRLEDAKQRLSTSNNSV
QSIANMVGYKDSFTFSKAFKRYSGASPSYYRKSIGI

Specific function: Involved in the regulation of the raffinose-operon [H]

COG id: COG2207

COG function: function code K; AraC-type DNA-binding domain-containing proteins

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 31504; Mature: 31504

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGEYKTLANKSFESNVLFFGQEACLPNYTYKGNNVRDSYVIHYIQEGKGTFAAANHPAT
CCCCCCHHHCCCCCCCEEEEECCCCCCCCEECCCCCCHHHEEEEEECCCCCEEECCCCEE
VLKAGDIFILPKGTPCFYQADNDQPWKYFWIGFSGIRIEAMLSGSLLAQKCYLRQVQNGQ
EEEECCEEEEECCCCEEEECCCCCCEEEEEEEECCEEEEEECCHHHHHHHHHHHHHCCCC
FYADLSELYKVLHIPNSLINDVLLGSLIYRMFYDLLRWYPADATNIKVKSTEQFNLAVSY
EEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
LQENYSTGCTIMDLCHYLNLSRSYLYTLFKTHANTSPQKLLTKLRLEDAKQRLSTSNNSV
HHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
QSIANMVGYKDSFTFSKAFKRYSGASPSYYRKSIGI
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MNGEYKTLANKSFESNVLFFGQEACLPNYTYKGNNVRDSYVIHYIQEGKGTFAAANHPAT
CCCCCCHHHCCCCCCCEEEEECCCCCCCCEECCCCCCHHHEEEEEECCCCCEEECCCCEE
VLKAGDIFILPKGTPCFYQADNDQPWKYFWIGFSGIRIEAMLSGSLLAQKCYLRQVQNGQ
EEEECCEEEEECCCCEEEECCCCCCEEEEEEEECCEEEEEECCHHHHHHHHHHHHHCCCC
FYADLSELYKVLHIPNSLINDVLLGSLIYRMFYDLLRWYPADATNIKVKSTEQFNLAVSY
EEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
LQENYSTGCTIMDLCHYLNLSRSYLYTLFKTHANTSPQKLLTKLRLEDAKQRLSTSNNSV
HHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHH
QSIANMVGYKDSFTFSKAFKRYSGASPSYYRKSIGI
HHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA