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The map label for this gene is insI1 [C]

Identifier: 116492298

GI number: 116492298

Start: 552310

End: 553239

Strand: Reverse

Name: insI1 [C]

Synonym: PEPE_0498

Alternate gene names: 116492298

Gene position: 553239-552310 (Counterclockwise)

Preceding gene: 116492299

Following gene: 116492296

Centisome position: 30.19

GC content: 40.54

Gene sequence:

>930_bases
TTGTCTAGTATAACCTATTCTGAACGAATTAAAATTGAAACCTTTTGTGAGCTAGGACTGACCAATATCCAAATGGCTGA
GCGGTTAAAACAATCTCCGTCTACGATTTCTTATGAATTATCTCGTTGTCAACCTTATCAAGCTGAATTAGCACAGGCCA
ACGCTGAATATAAACGGGCGCATTGTGGCCGAAAAACTAAACTGAATGCCAAATTAAAGCAAACCATTCTAAACCATTTA
CGTCTAAGCTGGTCACCGGAAGTGATTGCGCATGAGCTTAAACTAGCCACTAAATCTATTTATAATTGGCTATATCAGGG
GAAAATTGAATTCTCCTTGAGTGAACTACCTGAACATGGGCTACGCCAACGGCGTAACCTTGACCAACGTTCTAAATATA
ATCAATCATTAGGGCGTTCAATTGAACAACGACCAATTGTGATTAACCAACGTAACCGCATCGGTGATTTTGAAATAGAT
ACAGTGGTTGGCCCTCGTGGACATAGCAAGGCTGCTTTATTAACGCTAATTGATCGCAAATCACGTTTTCTTTGGGCTTA
CCGATTAAAAGATCGGACAGCGGCGACTGTTAATGAAGCCCTAACTATGTTTCTAACAACTTTTAAGAGACCGGTACACA
CCTTTACTGTCGATCGTGGAACTGAGTTTAGCAATCTAGTGTCATTTGAACCACAATACGGTATTCAGACCTATTACTGC
CATGCTTACACGCCCGCTGAACGCGGCAGTAATGAACGATTCAATCGGAACTTACGTTATTTTTACCCTAAGGGGACTTG
TTTTGAGCACATTAGTGCTCAAGATTTAACAACGACGTTACTCGAAATTAACCAACGCCCCCTTAAAGTACTTAACTGGC
AAACACCTTACCAAGTGATGCTGACTAATTTGTCAAAAAATTCGGATTAA

Upstream 100 bases:

>100_bases
TGGTAGATTGTAAAATTAATCCGAACGCTGTTCGGACAAAAAAGATCAGCTTCCTTTAAAATGGTGTTTACCACAAACCC
ATTTTTTAGGAGCTGATCTT

Downstream 100 bases:

>100_bases
ATTTGCAATCTACCATATTAATAAACAGCTAATATAGATAGTTCCTCCAATTTAAAAATAAATTTTGCTTAAAAACTAGT
TAAACCAAGTGATAATTCTT

Product: IS30 family transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSSITYSERIKIETFCELGLTNIQMAERLKQSPSTISYELSRCQPYQAELAQANAEYKRAHCGRKTKLNAKLKQTILNHL
RLSWSPEVIAHELKLATKSIYNWLYQGKIEFSLSELPEHGLRQRRNLDQRSKYNQSLGRSIEQRPIVINQRNRIGDFEID
TVVGPRGHSKAALLTLIDRKSRFLWAYRLKDRTAATVNEALTMFLTTFKRPVHTFTVDRGTEFSNLVSFEPQYGIQTYYC
HAYTPAERGSNERFNRNLRYFYPKGTCFEHISAQDLTTTLLEINQRPLKVLNWQTPYQVMLTNLSKNSD

Sequences:

>Translated_309_residues
MSSITYSERIKIETFCELGLTNIQMAERLKQSPSTISYELSRCQPYQAELAQANAEYKRAHCGRKTKLNAKLKQTILNHL
RLSWSPEVIAHELKLATKSIYNWLYQGKIEFSLSELPEHGLRQRRNLDQRSKYNQSLGRSIEQRPIVINQRNRIGDFEID
TVVGPRGHSKAALLTLIDRKSRFLWAYRLKDRTAATVNEALTMFLTTFKRPVHTFTVDRGTEFSNLVSFEPQYGIQTYYC
HAYTPAERGSNERFNRNLRYFYPKGTCFEHISAQDLTTTLLEINQRPLKVLNWQTPYQVMLTNLSKNSD
>Mature_308_residues
SSITYSERIKIETFCELGLTNIQMAERLKQSPSTISYELSRCQPYQAELAQANAEYKRAHCGRKTKLNAKLKQTILNHLR
LSWSPEVIAHELKLATKSIYNWLYQGKIEFSLSELPEHGLRQRRNLDQRSKYNQSLGRSIEQRPIVINQRNRIGDFEIDT
VVGPRGHSKAALLTLIDRKSRFLWAYRLKDRTAATVNEALTMFLTTFKRPVHTFTVDRGTEFSNLVSFEPQYGIQTYYCH
AYTPAERGSNERFNRNLRYFYPKGTCFEHISAQDLTTTLLEINQRPLKVLNWQTPYQVMLTNLSKNSD

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=326, Percent_Identity=30.0613496932515, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1790736, Length=326, Percent_Identity=30.0613496932515, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1787671, Length=326, Percent_Identity=30.0613496932515, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 36011; Mature: 35879

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSITYSERIKIETFCELGLTNIQMAERLKQSPSTISYELSRCQPYQAELAQANAEYKRA
CCCCCCHHCEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHCCHHHHHH
HCGRKTKLNAKLKQTILNHLRLSWSPEVIAHELKLATKSIYNWLYQGKIEFSLSELPEHG
HCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHCCHHH
LRQRRNLDQRSKYNQSLGRSIEQRPIVINQRNRIGDFEIDTVVGPRGHSKAALLTLIDRK
HHHHHCCHHHHHHHHHHCCCHHHCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCC
SRFLWAYRLKDRTAATVNEALTMFLTTFKRPVHTFTVDRGTEFSNLVSFEPQYGIQTYYC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEECCCCCCCEEEEE
HAYTPAERGSNERFNRNLRYFYPKGTCFEHISAQDLTTTLLEINQRPLKVLNWQTPYQVM
EEECHHHCCCCHHHCCCCEEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
LTNLSKNSD
EEHHCCCCC
>Mature Secondary Structure 
SSITYSERIKIETFCELGLTNIQMAERLKQSPSTISYELSRCQPYQAELAQANAEYKRA
CCCCCHHCEEHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHCCCHHHHHHHCCHHHHHH
HCGRKTKLNAKLKQTILNHLRLSWSPEVIAHELKLATKSIYNWLYQGKIEFSLSELPEHG
HCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEHHHCCHHH
LRQRRNLDQRSKYNQSLGRSIEQRPIVINQRNRIGDFEIDTVVGPRGHSKAALLTLIDRK
HHHHHCCHHHHHHHHHHCCCHHHCCEEEECCCCCCCEEEEEEECCCCCCCHHHHHHHHCC
SRFLWAYRLKDRTAATVNEALTMFLTTFKRPVHTFTVDRGTEFSNLVSFEPQYGIQTYYC
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHEECCCCCCCEEEEE
HAYTPAERGSNERFNRNLRYFYPKGTCFEHISAQDLTTTLLEINQRPLKVLNWQTPYQVM
EEECHHHCCCCHHHCCCCEEECCCCCHHHHCCHHHHHHHHHHHCCCCCEEECCCCCHHHH
LTNLSKNSD
EEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8393068 [H]