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The map label for this gene is dhaT [H]

Identifier: 116492080

GI number: 116492080

Start: 321556

End: 322725

Strand: Reverse

Name: dhaT [H]

Synonym: PEPE_0276

Alternate gene names: 116492080

Gene position: 322725-321556 (Counterclockwise)

Preceding gene: 116492081

Following gene: 116492066

Centisome position: 17.61

GC content: 41.79

Gene sequence:

>1170_bases
ATGAAAGAAAATTTTGATTTCCTTATGCCTAGTGTCAACTTCTTTGGCTCTGGAGTTATTGCAAAGATTGGTGACCGCGC
TAAGATGGTCAATATGTCTAAAGTATTGATCGTTACTGATAAATTTTTGGAAAAAATGGAAAATGGACCCGTTGAACAAA
CCCTTCGTTCACTTGATCAAGCGGGTGTCGATTACGTTATTTACAACGGAGTAGAGCCTAATCCTAAAGTTAGAAATATC
CAAGAAGCAAAACAAGTTTATTTGGATAATGACTGTGATGGAATTATCACTATTGGTGGTGGTTCAGCTCATGATGCTGG
AAAAGGTACTGGTATTATTTTGACGAATGGCGACGATATCACCAAACTTGCTGGAATCGAAACGCTCGAAAATGCACTTC
CACCATTGATCGCGGTTAATACTACTGCAGGAACTGCTTCAGAATTAACTCGTCACTGCGTTATTACCAACGAAGAAACG
CATCTTAAGTTCGTAGTGGTTTCGTGGCGTAATGTTCCCCTCGTATCATTTAATGATCCAAAATTAATGCTCGGGGTTCC
ACCTGCTCTAACTGCTGCAACTGGGATGGACACATTTGTCCAAGCCATTGAACCTTACGTTTCTACTAACCGCAACGATA
TTACTGATGGTCAATGTCTCCAAGCTATCAAGTTAGTGGAAGAAAGCCTTCGTGAAGCTGTATCTAACGGTGAAAACCTA
GAAGCTAGAACTAAGATGGTAGAAGCTGAAATGTTGGGAGGGATGGCTTTCAATAATGCCGATCTTGGTTACGTACATGC
TATGGCCCATCAATTAGGTGGACAATACGACGCTCCTCATGGCGTTTGCTGTGCTATCCTATTACCAATTGTAGAACGCT
TCAACTTAGTTGCTTGCCCTGATCGTTTCGCAGACTTAGCTGTCGCCATGGGTGAAAATATCGACGGCCTTTCCATTAGA
GCTGCCGCTGATAAAGCTATTGATGCCATGCAACAACTTAACGACGATGTTGGTATTCCAAGAAGTATCAAAGATTTGGG
TGCTGATCCTAAAGATTTCGAACTAATGGCTGAAAATGCTTTGAAGGATGGCAATGCCTTCTCCAACCCACGCAAAGCTA
CTAAGCAACAAATTGTGGAACTTTTCCAACAAGCTTACGACGCAAAATAA

Upstream 100 bases:

>100_bases
AAACGTTTAACTTGCGATTATATCAACGATTGTAAACGGATACATTACGTGTTAATATTAGCTCGTAAGTAAGAAAATAC
ATAAGAAGAAGGCGTTTAAA

Downstream 100 bases:

>100_bases
TTACTCAACAAAAAAAGATGAACTGAGATTTCTGATTCAGAATTCTCGTTCATCTTTTTTATTTATTTGGCAACTTTTTT
ATTACTTTTAACACTAAAGG

Product: 1,3-propanediol dehydrogenase

Products: NA

Alternate protein names: 1,3-propanediol oxidoreductase; 3-hydroxypropionaldehyde reductase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKENFDFLMPSVNFFGSGVIAKIGDRAKMVNMSKVLIVTDKFLEKMENGPVEQTLRSLDQAGVDYVIYNGVEPNPKVRNI
QEAKQVYLDNDCDGIITIGGGSAHDAGKGTGIILTNGDDITKLAGIETLENALPPLIAVNTTAGTASELTRHCVITNEET
HLKFVVVSWRNVPLVSFNDPKLMLGVPPALTAATGMDTFVQAIEPYVSTNRNDITDGQCLQAIKLVEESLREAVSNGENL
EARTKMVEAEMLGGMAFNNADLGYVHAMAHQLGGQYDAPHGVCCAILLPIVERFNLVACPDRFADLAVAMGENIDGLSIR
AAADKAIDAMQQLNDDVGIPRSIKDLGADPKDFELMAENALKDGNAFSNPRKATKQQIVELFQQAYDAK

Sequences:

>Translated_389_residues
MKENFDFLMPSVNFFGSGVIAKIGDRAKMVNMSKVLIVTDKFLEKMENGPVEQTLRSLDQAGVDYVIYNGVEPNPKVRNI
QEAKQVYLDNDCDGIITIGGGSAHDAGKGTGIILTNGDDITKLAGIETLENALPPLIAVNTTAGTASELTRHCVITNEET
HLKFVVVSWRNVPLVSFNDPKLMLGVPPALTAATGMDTFVQAIEPYVSTNRNDITDGQCLQAIKLVEESLREAVSNGENL
EARTKMVEAEMLGGMAFNNADLGYVHAMAHQLGGQYDAPHGVCCAILLPIVERFNLVACPDRFADLAVAMGENIDGLSIR
AAADKAIDAMQQLNDDVGIPRSIKDLGADPKDFELMAENALKDGNAFSNPRKATKQQIVELFQQAYDAK
>Mature_389_residues
MKENFDFLMPSVNFFGSGVIAKIGDRAKMVNMSKVLIVTDKFLEKMENGPVEQTLRSLDQAGVDYVIYNGVEPNPKVRNI
QEAKQVYLDNDCDGIITIGGGSAHDAGKGTGIILTNGDDITKLAGIETLENALPPLIAVNTTAGTASELTRHCVITNEET
HLKFVVVSWRNVPLVSFNDPKLMLGVPPALTAATGMDTFVQAIEPYVSTNRNDITDGQCLQAIKLVEESLREAVSNGENL
EARTKMVEAEMLGGMAFNNADLGYVHAMAHQLGGQYDAPHGVCCAILLPIVERFNLVACPDRFADLAVAMGENIDGLSIR
AAADKAIDAMQQLNDDVGIPRSIKDLGADPKDFELMAENALKDGNAFSNPRKATKQQIVELFQQAYDAK

Specific function: Most active with substrates containing two primary alcohol groups separated by one or two carbon atoms. In the physiological direction, 3-hydroxypropionaldehyde is the preferred substrate [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=397, Percent_Identity=25.1889168765743, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI48994951, Length=379, Percent_Identity=43.7994722955145, Blast_Score=330, Evalue=1e-91,
Organism=Escherichia coli, GI1789163, Length=375, Percent_Identity=39.7333333333333, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082107, Length=381, Percent_Identity=35.1706036745407, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1787493, Length=409, Percent_Identity=29.8288508557457, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1789386, Length=361, Percent_Identity=25.4847645429363, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=414, Percent_Identity=24.3961352657005, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321181, Length=385, Percent_Identity=42.0779220779221, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI24657991, Length=426, Percent_Identity=25.8215962441315, Blast_Score=124, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.202 [H]

Molecular weight: Translated: 42057; Mature: 42057

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENFDFLMPSVNFFGSGVIAKIGDRAKMVNMSKVLIVTDKFLEKMENGPVEQTLRSLDQ
CCCCCCHHCCCHHHHCCCHHHHHCCCHHEECCCEEEEEEHHHHHHHCCCCHHHHHHHHHH
AGVDYVIYNGVEPNPKVRNIQEAKQVYLDNDCDGIITIGGGSAHDAGKGTGIILTNGDDI
CCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCH
TKLAGIETLENALPPLIAVNTTAGTASELTRHCVITNEETHLKFVVVSWRNVPLVSFNDP
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEEEECCCCEEEEEEEEECCCEEEEECCC
KLMLGVPPALTAATGMDTFVQAIEPYVSTNRNDITDGQCLQAIKLVEESLREAVSNGENL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EARTKMVEAEMLGGMAFNNADLGYVHAMAHQLGGQYDAPHGVCCAILLPIVERFNLVACP
HHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEECC
DRFADLAVAMGENIDGLSIRAAADKAIDAMQQLNDDVGIPRSIKDLGADPKDFELMAENA
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHH
LKDGNAFSNPRKATKQQIVELFQQAYDAK
HCCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKENFDFLMPSVNFFGSGVIAKIGDRAKMVNMSKVLIVTDKFLEKMENGPVEQTLRSLDQ
CCCCCCHHCCCHHHHCCCHHHHHCCCHHEECCCEEEEEEHHHHHHHCCCCHHHHHHHHHH
AGVDYVIYNGVEPNPKVRNIQEAKQVYLDNDCDGIITIGGGSAHDAGKGTGIILTNGDDI
CCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCH
TKLAGIETLENALPPLIAVNTTAGTASELTRHCVITNEETHLKFVVVSWRNVPLVSFNDP
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHEEEECCCCEEEEEEEEECCCEEEEECCC
KLMLGVPPALTAATGMDTFVQAIEPYVSTNRNDITDGQCLQAIKLVEESLREAVSNGENL
EEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
EARTKMVEAEMLGGMAFNNADLGYVHAMAHQLGGQYDAPHGVCCAILLPIVERFNLVACP
HHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEECC
DRFADLAVAMGENIDGLSIRAAADKAIDAMQQLNDDVGIPRSIKDLGADPKDFELMAENA
HHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHH
LKDGNAFSNPRKATKQQIVELFQQAYDAK
HCCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7721705 [H]