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The map label for this gene is lacL [H]

Identifier: 116492013

GI number: 116492013

Start: 243306

End: 245186

Strand: Reverse

Name: lacL [H]

Synonym: PEPE_0205

Alternate gene names: 116492013

Gene position: 245186-243306 (Counterclockwise)

Preceding gene: 116492020

Following gene: 116492012

Centisome position: 13.38

GC content: 41.1

Gene sequence:

>1881_bases
ATGCAAGCAAATCTTCAGTGGCTTGATGATCCTGAAGTTTTTCGAGTCAATCAACTACCTGCCCATAGCGACCACCATTA
CTATCGTGATTTAGACGAAATCCGTACAGGTAGCAGCTTTATTAAAAGTCTCAACGGTCCTTGGCGTTTTCACTTTGCGT
CCACCCCGTCTGAACGTCCGCTCCATTTTTATTCACCTGATTTTGATAGTGCTGATTTTGATACGATCCAAGTACCAGGG
CATATCGAATTAGCAGGCTACAGTCAAATTCAATATATCAACACTCTCTACCCTTGGGAAGGAAAAATTTATCGTCGTCC
CCCTTACACACTTAATCAAGACCAATTAGAAGTTGGATTATTTAGCGATGCTGCTGATAACAGTGTCGGCTCTTATTTAA
AACACTTTGACCTTGATGATTCATTTAAAAATCAGCGAGTTATCATTCAATTTCAAGGAGTTGAAGAAGCATTATACGTT
TGGTTGAATGGCCATTTTATTGGTTATGCTGAAGATAGCTTCACTCCTTCAGAATTTGATTTAACCCCTTATCTTCAAGA
CCAAGATAATCTTTTAGCTGTACAGGTTTATAAAAGAAGTACGGCAGCCTTTATTGAAGATCAAGATATGTACCGCTTTT
CTGGTATTTTTCGTGATGTTAATCTCCTCGCTCTTCCGGCCGCCCATGTTGTGGATTTAGACATCAAACCGGAGGTAAAA
GATGATTTTGAGCGCGGTACCTTGGCAGTTACTGCTGACGTTTCTGGTACTGATGCTATCTTAGAACTAACCGTTACGGA
TCAAAATAAACAGGTAGTGGCCAGTCAGACCCAAAGCGGAACGGGTAAGGTCCTGTTTGATTCCATGCCGTTTGATAATT
TGAAACTATGGTCACCAACAAACCCCACCTTGTATCAGTTGTTGATTAAAGTCTACAACAATCAGCATCAGCTCTTGGAA
GTCGTTCCTTATCAATTTGGTTTTCGGCGAGTGGAATTACGTGAGGATAAAGTCATTTACGTTAATAACCAACGTTTGAT
TCTCAACGGGGTTAATCGCCACGAGTGGAATGCACATAGTGGGCGTGTCATCACCATGCAAGATATGCAGAACGATATTC
AGACAATGCTTGATAATAATATCAACGCTGTTCGAACTTGCCATTATCCTGATCAACTACCGTGGTACGAACTATGCGAT
CAAGCCGGTCTTTACGTTATGGCTGAAAATAACCTAGAATCTCATGGTTCATGGCAAAAAATGGGGGCTGTTGAACCTTC
CTATAACGTTCCTGGGGATAATCCTCATTGGTTAGCAGCAGTCGTTGATCGGGCTCGTTCCAACTATGAATGGTTCAAAA
ACCACCCATCCATCATTTTCTGGTCATTAGGCAACGAATCCTTTGCTGGTGAAGATATTGCGGCTATGCAAACTTTCTAT
AAAGAACATGACGCATCTCGACTGGTTCATTATGAAGGTGTCGTTCGTACTCCAGAATTACGCGATCGTATTTCTGATGT
AGAAAGCCGTATGTACGAAACTCCAGCTAACATCGCTAGCTATCTAGAAAATAATCCCGATAAACCTTTCTTAAACTGTG
AATATATGCATGATATGGGAAACTCTTTAGGGGGCATGCAATCGTATAATGATTTAATTGATAAATACCCAATGTATCAA
GGTGGCTTCATTTGGGACTTCATTGATCAAGCTCTGTTAGTTCATGATCCAATCACCCAGCAAGACGTCCTACGATACGG
TGGTGATTTCGATGAACGCCATTCCGACTATGAGTTTTCTGGTGATGGATTACTATTTGCTGATCGAACCCCTAAACCAG
CAATGCAGGAGGTGAAATACTATTATGGATTACACAAATAA

Upstream 100 bases:

>100_bases
TTGGTTAAATTTTAACTAAAACAACGTATACAAATTTAGGGTTATGTTCTATTATTTCATTGTAAGCGTTTTATATTTTG
GTTACGAAAGGAGTTTTATT

Downstream 100 bases:

>100_bases
TCAATTACACGTCATATATGGCGATGCCAGTTTAGGATTAGAAGGTCCTAACTTTCACTACCTTTTTAGTTACGAACGAG
GCGGGCTTGAATCTTTAGTG

Product: Beta-galactosidase

Products: NA

Alternate protein names: Beta-gal large subunit; Lactase large subunit [H]

Number of amino acids: Translated: 626; Mature: 626

Protein sequence:

>626_residues
MQANLQWLDDPEVFRVNQLPAHSDHHYYRDLDEIRTGSSFIKSLNGPWRFHFASTPSERPLHFYSPDFDSADFDTIQVPG
HIELAGYSQIQYINTLYPWEGKIYRRPPYTLNQDQLEVGLFSDAADNSVGSYLKHFDLDDSFKNQRVIIQFQGVEEALYV
WLNGHFIGYAEDSFTPSEFDLTPYLQDQDNLLAVQVYKRSTAAFIEDQDMYRFSGIFRDVNLLALPAAHVVDLDIKPEVK
DDFERGTLAVTADVSGTDAILELTVTDQNKQVVASQTQSGTGKVLFDSMPFDNLKLWSPTNPTLYQLLIKVYNNQHQLLE
VVPYQFGFRRVELREDKVIYVNNQRLILNGVNRHEWNAHSGRVITMQDMQNDIQTMLDNNINAVRTCHYPDQLPWYELCD
QAGLYVMAENNLESHGSWQKMGAVEPSYNVPGDNPHWLAAVVDRARSNYEWFKNHPSIIFWSLGNESFAGEDIAAMQTFY
KEHDASRLVHYEGVVRTPELRDRISDVESRMYETPANIASYLENNPDKPFLNCEYMHDMGNSLGGMQSYNDLIDKYPMYQ
GGFIWDFIDQALLVHDPITQQDVLRYGGDFDERHSDYEFSGDGLLFADRTPKPAMQEVKYYYGLHK

Sequences:

>Translated_626_residues
MQANLQWLDDPEVFRVNQLPAHSDHHYYRDLDEIRTGSSFIKSLNGPWRFHFASTPSERPLHFYSPDFDSADFDTIQVPG
HIELAGYSQIQYINTLYPWEGKIYRRPPYTLNQDQLEVGLFSDAADNSVGSYLKHFDLDDSFKNQRVIIQFQGVEEALYV
WLNGHFIGYAEDSFTPSEFDLTPYLQDQDNLLAVQVYKRSTAAFIEDQDMYRFSGIFRDVNLLALPAAHVVDLDIKPEVK
DDFERGTLAVTADVSGTDAILELTVTDQNKQVVASQTQSGTGKVLFDSMPFDNLKLWSPTNPTLYQLLIKVYNNQHQLLE
VVPYQFGFRRVELREDKVIYVNNQRLILNGVNRHEWNAHSGRVITMQDMQNDIQTMLDNNINAVRTCHYPDQLPWYELCD
QAGLYVMAENNLESHGSWQKMGAVEPSYNVPGDNPHWLAAVVDRARSNYEWFKNHPSIIFWSLGNESFAGEDIAAMQTFY
KEHDASRLVHYEGVVRTPELRDRISDVESRMYETPANIASYLENNPDKPFLNCEYMHDMGNSLGGMQSYNDLIDKYPMYQ
GGFIWDFIDQALLVHDPITQQDVLRYGGDFDERHSDYEFSGDGLLFADRTPKPAMQEVKYYYGLHK
>Mature_626_residues
MQANLQWLDDPEVFRVNQLPAHSDHHYYRDLDEIRTGSSFIKSLNGPWRFHFASTPSERPLHFYSPDFDSADFDTIQVPG
HIELAGYSQIQYINTLYPWEGKIYRRPPYTLNQDQLEVGLFSDAADNSVGSYLKHFDLDDSFKNQRVIIQFQGVEEALYV
WLNGHFIGYAEDSFTPSEFDLTPYLQDQDNLLAVQVYKRSTAAFIEDQDMYRFSGIFRDVNLLALPAAHVVDLDIKPEVK
DDFERGTLAVTADVSGTDAILELTVTDQNKQVVASQTQSGTGKVLFDSMPFDNLKLWSPTNPTLYQLLIKVYNNQHQLLE
VVPYQFGFRRVELREDKVIYVNNQRLILNGVNRHEWNAHSGRVITMQDMQNDIQTMLDNNINAVRTCHYPDQLPWYELCD
QAGLYVMAENNLESHGSWQKMGAVEPSYNVPGDNPHWLAAVVDRARSNYEWFKNHPSIIFWSLGNESFAGEDIAAMQTFY
KEHDASRLVHYEGVVRTPELRDRISDVESRMYETPANIASYLENNPDKPFLNCEYMHDMGNSLGGMQSYNDLIDKYPMYQ
GGFIWDFIDQALLVHDPITQQDVLRYGGDFDERHSDYEFSGDGLLFADRTPKPAMQEVKYYYGLHK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=386, Percent_Identity=22.7979274611399, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1786539, Length=635, Percent_Identity=36.5354330708661, Blast_Score=366, Evalue=1e-102,
Organism=Escherichia coli, GI48994920, Length=595, Percent_Identity=33.9495798319328, Blast_Score=330, Evalue=2e-91,
Organism=Escherichia coli, GI1787903, Length=546, Percent_Identity=23.2600732600733, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24655438, Length=555, Percent_Identity=21.981981981982, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI62471735, Length=555, Percent_Identity=21.981981981982, Blast_Score=82, Evalue=9e-16,
Organism=Drosophila melanogaster, GI19922582, Length=555, Percent_Identity=21.981981981982, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 72334; Mature: 72334

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1 ; PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQANLQWLDDPEVFRVNQLPAHSDHHYYRDLDEIRTGSSFIKSLNGPWRFHFASTPSERP
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCHHHHHCCHHHHHHCCCCEEEEECCCCCCCC
LHFYSPDFDSADFDTIQVPGHIELAGYSQIQYINTLYPWEGKIYRRPPYTLNQDQLEVGL
EEEECCCCCCCCCCEEECCCEEEECCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEE
FSDAADNSVGSYLKHFDLDDSFKNQRVIIQFQGVEEALYVWLNGHFIGYAEDSFTPSEFD
ECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEEEECCCCCCCCCC
LTPYLQDQDNLLAVQVYKRSTAAFIEDQDMYRFSGIFRDVNLLALPAAHVVDLDIKPEVK
CCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCC
DDFERGTLAVTADVSGTDAILELTVTDQNKQVVASQTQSGTGKVLFDSMPFDNLKLWSPT
CCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCEEEECCC
NPTLYQLLIKVYNNQHQLLEVVPYQFGFRRVELREDKVIYVNNQRLILNGVNRHEWNAHS
CHHHHHHHHHHHCCCHHHHHHCCHHHCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCC
GRVITMQDMQNDIQTMLDNNINAVRTCHYPDQLPWYELCDQAGLYVMAENNLESHGSWQK
CEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCEEEEECCCCCCCCCHHH
MGAVEPSYNVPGDNPHWLAAVVDRARSNYEWFKNHPSIIFWSLGNESFAGEDIAAMQTFY
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
KEHDASRLVHYEGVVRTPELRDRISDVESRMYETPANIASYLENNPDKPFLNCEYMHDMG
HHHCCCCEEEECCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEHHHHHCC
NSLGGMQSYNDLIDKYPMYQGGFIWDFIDQALLVHDPITQQDVLRYGGDFDERHSDYEFS
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHCHHEECCCCCHHHHHHHCCCCCCCCCCCEEC
GDGLLFADRTPKPAMQEVKYYYGLHK
CCEEEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MQANLQWLDDPEVFRVNQLPAHSDHHYYRDLDEIRTGSSFIKSLNGPWRFHFASTPSERP
CCCCCCCCCCCCEEEEECCCCCCCCHHHHCHHHHHCCHHHHHHCCCCEEEEECCCCCCCC
LHFYSPDFDSADFDTIQVPGHIELAGYSQIQYINTLYPWEGKIYRRPPYTLNQDQLEVGL
EEEECCCCCCCCCCEEECCCEEEECCCCEEEEEEEECCCCCEEEECCCCCCCCCCEEEEE
FSDAADNSVGSYLKHFDLDDSFKNQRVIIQFQGVEEALYVWLNGHFIGYAEDSFTPSEFD
ECCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEEEECCEEEEEECCCCCCCCCC
LTPYLQDQDNLLAVQVYKRSTAAFIEDQDMYRFSGIFRDVNLLALPAAHVVDLDIKPEVK
CCCCCCCCCCEEEEEEEECCCCEEECCCHHHHHHHHHHCCEEEEECCCEEEEECCCCCCC
DDFERGTLAVTADVSGTDAILELTVTDQNKQVVASQTQSGTGKVLFDSMPFDNLKLWSPT
CCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCEEEECCC
NPTLYQLLIKVYNNQHQLLEVVPYQFGFRRVELREDKVIYVNNQRLILNGVNRHEWNAHS
CHHHHHHHHHHHCCCHHHHHHCCHHHCCEEEEECCCCEEEECCCEEEEECCCCCCCCCCC
GRVITMQDMQNDIQTMLDNNINAVRTCHYPDQLPWYELCDQAGLYVMAENNLESHGSWQK
CEEEEHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHCCEEEEECCCCCCCCCHHH
MGAVEPSYNVPGDNPHWLAAVVDRARSNYEWFKNHPSIIFWSLGNESFAGEDIAAMQTFY
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHH
KEHDASRLVHYEGVVRTPELRDRISDVESRMYETPANIASYLENNPDKPFLNCEYMHDMG
HHHCCCCEEEECCCEECHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEHHHHHCC
NSLGGMQSYNDLIDKYPMYQGGFIWDFIDQALLVHDPITQQDVLRYGGDFDERHSDYEFS
HHCCCCHHHHHHHHHCCCCCCCCHHHHHHCHHEECCCCCHHHHHHHCCCCCCCCCCCEEC
GDGLLFADRTPKPAMQEVKYYYGLHK
CCEEEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1624440 [H]