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The map label for this gene is fruA [H]

Identifier: 116491956

GI number: 116491956

Start: 167981

End: 169870

Strand: Direct

Name: fruA [H]

Synonym: PEPE_0142

Alternate gene names: 116491956

Gene position: 167981-169870 (Clockwise)

Preceding gene: 116491955

Following gene: 116491957

Centisome position: 9.17

GC content: 36.08

Gene sequence:

>1890_bases
ATGACAAAATATACAATTATTGCAGCTACTGGCTGTCCAACTGGAATTGCCCACACCTATATGGCCCAAGAAGCGTTAGA
AGAGGCCGCCAAGAAACGAGGAGTTTCAATTAAAGTAGAAACACATGGACAAAGCGGTACAGAAAATCAATTTACGCCTG
AAGAAATTAGCAACGCTAAAGGAGTCATTATAGCAGCTGATAAAGACGTTGATATTGAAAGATTTGATGGAAAAAGATTA
ATTAATGTTTCAGTAACTAAAGGAATGAAAGAACCGGATGAATTAGTTAGTAAGATATTGGACGATGATGTGCCGATATA
CCACAGTAATGGCCAATATAATAAAGAAGATCAAATCAGTACAACTAATGGAACTTTCTGGCACAGTATCTATGTAGACT
TAATGAACGGTGTTTCGCATATGCTTCCTTTGGTTGTTGCTGGAGGGGTATTAACAGCTATTTCCTTTTTCTGGGGTATC
AATTCTGCCGACCCGAATAGCGCTCAATTTAATTCATTTGCTCAACTGCTAAATACGATTGGCGGATTTGCAATGAATTT
GATGGTACCCGTTTTATGTGCCTATATTGCTGAAGCAATTGGAAAAAGATCTGGTTTAGTAGTTGGTTTTGCAACGGGAA
TGATTGCTTATACCAATGGTACTGGATTCTTAGGAGGAATTGTCGGAGGATTTCTTGCAGGATACACAGTGGTTTTATTA
CAGCATTTATTTAAAAAATTGCCTAAATCTTTAGATGGTTTAAAACAAATTTTTCTATTTCCGGTTATTGGAGTTTTTAT
TTCCGGTGCGATTATGTGGTATATGTCGGTACCGATGAAAGATATTAATACCGGCATGATGGCGTTTTTAAAAGGAATGG
AAAATTCTAGTCCATTAGTTTTGGGATTAATTATTGGAATTATGTGTGCTTCTGATATGGGTGGTCCAATCAATAAAGCT
GCATACCTAACTGGTACAGCTTTGCTTGCACAAGGAAATTACTTTTTCATGGCTGGAGTATCTGCAGCCTGCATAGCTCC
GCCATTAGCTACAGGCTTTTCAGTACTTTTTAACCGTAAAGCATACTCTGAAAATGAGCGAAGTGCTGGTTATGTTAACT
TCTTATTAGGATCAACGCATATTACAGAAGGAGCAATACCTTTTGCGGCTAAAAATCCGTTAATTAATATTCCAATTTTT
ATGGTTGGATCTTCCATCGCAGCAATTTTAACTTATACAACTAGAATTAGCGTTCCAGCACCACATGGTGGTTTTATCGT
TCTACCTTTGGTTAATAAACCATTTTTATGGGTTTTGTTTATTGTAATTGGAGCAATTGTTTCGGGGCTATTACTTTCAT
TAGCTGATGGTCATAAAGCTCACGTTGGAGAAAAAACATTAGAAGATAAAAGAGAAGTATCAAATGAGCGTAAAGTGAAT
GACGAAAGTTTAGATAGTATCCTCACTGTAGATAATATTAAAGTTAATGTTGATGTTGAATCTAGAGATGAACTATTACG
GTATCTATCAAACTTATCTGTAAGTAACGGATATAGTTCAGATGCAAATGCGGTATACAATAAATATTTAGCACGTGAGT
CAGCAGGATCTACCGGGATGGAAAAAGGCATCGCTATTCCACATGCTCAAGATAATACAATTAATACGTCTGCAATGTTA
GTGCTGAAATTAAAACAACCGATTGAATGGAAAACTTTTGATAACCAACCCGTTGATACGGTAATTTCGTTTTTAATTCC
TGAACGTGACCAAGGTAATCATTTACGATATCTTTCATCGGTTTCTAAGTTACTAATGCATGAAAGTTTTGTTCAAGAAC
TAAAAAATGCTAAATCGGTCGAAGAAATAGCGGCACTATTCAAAAAATAA

Upstream 100 bases:

>100_bases
CTGGAAGCTCAACTGCATTTAAAGCAGGATTAACTGATTTTTCAGACGTTGAGGAGTTAAACGAGCAAATTAAGATTGAA
ACTGTTAGGAGGGATAGTAA

Downstream 100 bases:

>100_bases
AAATTAAGGCTACTTCAGTTTAAAATGAAAGCGTTTGACAAAGTGGTACCAACCAGTTAAAATAGGTTTTGTTGAATGAT
ACCAACCAGTACAGATGAGA

Product: phosphotransferase system fructose-specific component IIB

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MTKYTIIAATGCPTGIAHTYMAQEALEEAAKKRGVSIKVETHGQSGTENQFTPEEISNAKGVIIAADKDVDIERFDGKRL
INVSVTKGMKEPDELVSKILDDDVPIYHSNGQYNKEDQISTTNGTFWHSIYVDLMNGVSHMLPLVVAGGVLTAISFFWGI
NSADPNSAQFNSFAQLLNTIGGFAMNLMVPVLCAYIAEAIGKRSGLVVGFATGMIAYTNGTGFLGGIVGGFLAGYTVVLL
QHLFKKLPKSLDGLKQIFLFPVIGVFISGAIMWYMSVPMKDINTGMMAFLKGMENSSPLVLGLIIGIMCASDMGGPINKA
AYLTGTALLAQGNYFFMAGVSAACIAPPLATGFSVLFNRKAYSENERSAGYVNFLLGSTHITEGAIPFAAKNPLINIPIF
MVGSSIAAILTYTTRISVPAPHGGFIVLPLVNKPFLWVLFIVIGAIVSGLLLSLADGHKAHVGEKTLEDKREVSNERKVN
DESLDSILTVDNIKVNVDVESRDELLRYLSNLSVSNGYSSDANAVYNKYLARESAGSTGMEKGIAIPHAQDNTINTSAML
VLKLKQPIEWKTFDNQPVDTVISFLIPERDQGNHLRYLSSVSKLLMHESFVQELKNAKSVEEIAALFKK

Sequences:

>Translated_629_residues
MTKYTIIAATGCPTGIAHTYMAQEALEEAAKKRGVSIKVETHGQSGTENQFTPEEISNAKGVIIAADKDVDIERFDGKRL
INVSVTKGMKEPDELVSKILDDDVPIYHSNGQYNKEDQISTTNGTFWHSIYVDLMNGVSHMLPLVVAGGVLTAISFFWGI
NSADPNSAQFNSFAQLLNTIGGFAMNLMVPVLCAYIAEAIGKRSGLVVGFATGMIAYTNGTGFLGGIVGGFLAGYTVVLL
QHLFKKLPKSLDGLKQIFLFPVIGVFISGAIMWYMSVPMKDINTGMMAFLKGMENSSPLVLGLIIGIMCASDMGGPINKA
AYLTGTALLAQGNYFFMAGVSAACIAPPLATGFSVLFNRKAYSENERSAGYVNFLLGSTHITEGAIPFAAKNPLINIPIF
MVGSSIAAILTYTTRISVPAPHGGFIVLPLVNKPFLWVLFIVIGAIVSGLLLSLADGHKAHVGEKTLEDKREVSNERKVN
DESLDSILTVDNIKVNVDVESRDELLRYLSNLSVSNGYSSDANAVYNKYLARESAGSTGMEKGIAIPHAQDNTINTSAML
VLKLKQPIEWKTFDNQPVDTVISFLIPERDQGNHLRYLSSVSKLLMHESFVQELKNAKSVEEIAALFKK
>Mature_628_residues
TKYTIIAATGCPTGIAHTYMAQEALEEAAKKRGVSIKVETHGQSGTENQFTPEEISNAKGVIIAADKDVDIERFDGKRLI
NVSVTKGMKEPDELVSKILDDDVPIYHSNGQYNKEDQISTTNGTFWHSIYVDLMNGVSHMLPLVVAGGVLTAISFFWGIN
SADPNSAQFNSFAQLLNTIGGFAMNLMVPVLCAYIAEAIGKRSGLVVGFATGMIAYTNGTGFLGGIVGGFLAGYTVVLLQ
HLFKKLPKSLDGLKQIFLFPVIGVFISGAIMWYMSVPMKDINTGMMAFLKGMENSSPLVLGLIIGIMCASDMGGPINKAA
YLTGTALLAQGNYFFMAGVSAACIAPPLATGFSVLFNRKAYSENERSAGYVNFLLGSTHITEGAIPFAAKNPLINIPIFM
VGSSIAAILTYTTRISVPAPHGGFIVLPLVNKPFLWVLFIVIGAIVSGLLLSLADGHKAHVGEKTLEDKREVSNERKVND
ESLDSILTVDNIKVNVDVESRDELLRYLSNLSVSNGYSSDANAVYNKYLARESAGSTGMEKGIAIPHAQDNTINTSAMLV
LKLKQPIEWKTFDNQPVDTVISFLIPERDQGNHLRYLSSVSKLLMHESFVQELKNAKSVEEIAALFKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=452, Percent_Identity=39.8230088495575, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1786951, Length=439, Percent_Identity=32.8018223234624, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87082348, Length=441, Percent_Identity=31.7460317460317, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1790386, Length=302, Percent_Identity=38.0794701986755, Blast_Score=189, Evalue=7e-49,
Organism=Escherichia coli, GI1788729, Length=386, Percent_Identity=27.2020725388601, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1790387, Length=102, Percent_Identity=50, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789597, Length=148, Percent_Identity=33.7837837837838, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI2367327, Length=139, Percent_Identity=23.7410071942446, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788730, Length=95, Percent_Identity=35.7894736842105, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790390, Length=97, Percent_Identity=38.1443298969072, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI48994992, Length=143, Percent_Identity=27.972027972028, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788726, Length=149, Percent_Identity=26.8456375838926, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 67993; Mature: 67862

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS51094 PTS_EIIA_TYPE_2 ; PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKYTIIAATGCPTGIAHTYMAQEALEEAAKKRGVSIKVETHGQSGTENQFTPEEISNAK
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCC
GVIIAADKDVDIERFDGKRLINVSVTKGMKEPDELVSKILDDDVPIYHSNGQYNKEDQIS
CEEEEECCCCCCEECCCCEEEEEEHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCC
TTNGTFWHSIYVDLMNGVSHMLPLVVAGGVLTAISFFWGINSADPNSAQFNSFAQLLNTI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
GGFAMNLMVPVLCAYIAEAIGKRSGLVVGFATGMIAYTNGTGFLGGIVGGFLAGYTVVLL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
QHLFKKLPKSLDGLKQIFLFPVIGVFISGAIMWYMSVPMKDINTGMMAFLKGMENSSPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHH
LGLIIGIMCASDMGGPINKAAYLTGTALLAQGNYFFMAGVSAACIAPPLATGFSVLFNRK
HHHHHHHHHHHCCCCCCCCHHHHHHHEEEECCCEEEEECCCHHHHCCHHHHHHHHHHCCH
AYSENERSAGYVNFLLGSTHITEGAIPFAAKNPLINIPIFMVGSSIAAILTYTTRISVPA
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHEEECCC
PHGGFIVLPLVNKPFLWVLFIVIGAIVSGLLLSLADGHKAHVGEKTLEDKREVSNERKVN
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCC
DESLDSILTVDNIKVNVDVESRDELLRYLSNLSVSNGYSSDANAVYNKYLARESAGSTGM
HHHHHHEEEECCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKGIAIPHAQDNTINTSAMLVLKLKQPIEWKTFDNQPVDTVISFLIPERDQGNHLRYLSS
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
VSKLLMHESFVQELKNAKSVEEIAALFKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure 
TKYTIIAATGCPTGIAHTYMAQEALEEAAKKRGVSIKVETHGQSGTENQFTPEEISNAK
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHCCCC
GVIIAADKDVDIERFDGKRLINVSVTKGMKEPDELVSKILDDDVPIYHSNGQYNKEDQIS
CEEEEECCCCCCEECCCCEEEEEEHHCCCCCHHHHHHHHHCCCCCEEECCCCCCCCCCCC
TTNGTFWHSIYVDLMNGVSHMLPLVVAGGVLTAISFFWGINSADPNSAQFNSFAQLLNTI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
GGFAMNLMVPVLCAYIAEAIGKRSGLVVGFATGMIAYTNGTGFLGGIVGGFLAGYTVVLL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEECCCCHHHHHHHHHHHHHHHHHH
QHLFKKLPKSLDGLKQIFLFPVIGVFISGAIMWYMSVPMKDINTGMMAFLKGMENSSPLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHH
LGLIIGIMCASDMGGPINKAAYLTGTALLAQGNYFFMAGVSAACIAPPLATGFSVLFNRK
HHHHHHHHHHHCCCCCCCCHHHHHHHEEEECCCEEEEECCCHHHHCCHHHHHHHHHHCCH
AYSENERSAGYVNFLLGSTHITEGAIPFAAKNPLINIPIFMVGSSIAAILTYTTRISVPA
HCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHEEECCC
PHGGFIVLPLVNKPFLWVLFIVIGAIVSGLLLSLADGHKAHVGEKTLEDKREVSNERKVN
CCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHCCC
DESLDSILTVDNIKVNVDVESRDELLRYLSNLSVSNGYSSDANAVYNKYLARESAGSTGM
HHHHHHEEEECCEEEEEECCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
EKGIAIPHAQDNTINTSAMLVLKLKQPIEWKTFDNQPVDTVISFLIPERDQGNHLRYLSS
CCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHH
VSKLLMHESFVQELKNAKSVEEIAALFKK
HHHHHHHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]