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The map label for this gene is ssb [H]

Identifier: 116491826

GI number: 116491826

Start: 9377

End: 9904

Strand: Direct

Name: ssb [H]

Synonym: PEPE_0008

Alternate gene names: 116491826

Gene position: 9377-9904 (Clockwise)

Preceding gene: 116491825

Following gene: 116491827

Centisome position: 0.51

GC content: 41.48

Gene sequence:

>528_bases
ATGATTAACCGAACAGTGCTTGTCGGACGCCTAACAAGGGACCCTGAACTACGATATACAGGTAGTGGAGCTGCAGTAGT
AACTTTTACAGTTGCTGTTAATCGTCAGTTTACTAATTCACAAGGTGAACGCGAAGCTGACTTTATTAATTGTGTTATGT
GGCGTAAAGCTGCAGAAAACTTTGCTAACTTCACTCGGAAGGGTTCATTAGTTGGTATCGACGGACGGATTCAAACCCGT
TCTTATGAAAACCAACAAGGCCAACGAGTTTATGTTACCGAGGTTGTAGCCGAAAACTTCTCTCTTCTTGAATCACGTTC
ATCTTCTGAACGTCGTCAAGGTGGAGAAAATAATTTTGGCAATAATCAATCTGCCCCTTCACAACAACCAACATCATCCG
GAAATCCATTTGACGGAGGACAATCGAATAATAGTGGTGCACAACAACCTAACAATTCGAACCCTAATGACCCATTTGCT
AATAGCGGTCAGTCAATTGATATTTCTGACGATGATTTACCATTCTAA

Upstream 100 bases:

>100_bases
ACGAATTTGATCGTCTATCTAAGATTGCTGACGGAATTCTTCGTCACATGATCGTTGTACGTTAATAATAACAACTAAAA
ACTAGGAAGGAGCGGATATT

Downstream 100 bases:

>100_bases
AATAATAGAGGAGGGCAATCATTATGGCATTTCAAAAACGTGGTGGCCGTCGTCGTCGTAAAGTCGACTACATTGCTGCA
AACCATATTGAATACATCGA

Product: single-stranded DNA-binding protein

Products: NA

Alternate protein names: SSB; Helix-destabilizing protein [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MINRTVLVGRLTRDPELRYTGSGAAVVTFTVAVNRQFTNSQGEREADFINCVMWRKAAENFANFTRKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVAENFSLLESRSSSERRQGGENNFGNNQSAPSQQPTSSGNPFDGGQSNNSGAQQPNNSNPNDPFA
NSGQSIDISDDDLPF

Sequences:

>Translated_175_residues
MINRTVLVGRLTRDPELRYTGSGAAVVTFTVAVNRQFTNSQGEREADFINCVMWRKAAENFANFTRKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVAENFSLLESRSSSERRQGGENNFGNNQSAPSQQPTSSGNPFDGGQSNNSGAQQPNNSNPNDPFA
NSGQSIDISDDDLPF
>Mature_175_residues
MINRTVLVGRLTRDPELRYTGSGAAVVTFTVAVNRQFTNSQGEREADFINCVMWRKAAENFANFTRKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVAENFSLLESRSSSERRQGGENNFGNNQSAPSQQPTSSGNPFDGGQSNNSGAQQPNNSNPNDPFA
NSGQSIDISDDDLPF

Specific function: This protein is essential for replication of the chromosome. It is also involved in DNA recombination and repair [H]

COG id: COG0629

COG function: function code L; Single-stranded DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SSB domain [H]

Homologues:

Organism=Escherichia coli, GI1790494, Length=98, Percent_Identity=37.7551020408163, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: 1622 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000424
- InterPro:   IPR011344 [H]

Pfam domain/function: PF00436 SSB [H]

EC number: NA

Molecular weight: Translated: 19175; Mature: 19175

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50935 SSB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRTVLVGRLTRDPELRYTGSGAAVVTFTVAVNRQFTNSQGEREADFINCVMWRKAAEN
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
FANFTRKGSLVGIDGRIQTRSYENQQGQRVYVTEVVAENFSLLESRSSSERRQGGENNFG
HHHHHHCCCEEEECCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCCCCC
NNQSAPSQQPTSSGNPFDGGQSNNSGAQQPNNSNPNDPFANSGQSIDISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC
>Mature Secondary Structure
MINRTVLVGRLTRDPELRYTGSGAAVVTFTVAVNRQFTNSQGEREADFINCVMWRKAAEN
CCCCEEEEEECCCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHH
FANFTRKGSLVGIDGRIQTRSYENQQGQRVYVTEVVAENFSLLESRSSSERRQGGENNFG
HHHHHHCCCEEEECCEEECCCCCCCCCCEEEEHHHHHHHHHHHHHCCHHHHHCCCCCCCC
NNQSAPSQQPTSSGNPFDGGQSNNSGAQQPNNSNPNDPFANSGQSIDISDDDLPF
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663927 [H]