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The map label for this gene is pykF [H]

Identifier: 116332670

GI number: 116332670

Start: 284675

End: 286129

Strand: Reverse

Name: pykF [H]

Synonym: LBJ_4253

Alternate gene names: 116332670

Gene position: 286129-284675 (Counterclockwise)

Preceding gene: 116332671

Following gene: 116332666

Centisome position: 95.45

GC content: 46.12

Gene sequence:

>1455_bases
ATGAAGAGCGAATTCTCCGTAATTAGAAAAACGAAAATTATTTGTACCATCGGGCCTGCAACCGCCGATAAAAAAATGAT
TCAGGCGCTTGCCGAAGCCGGGATGAACGTGGCGCGTCTAAACATGTCCCACGGAAATCACGATTTTCATAGAAGTATTA
TTCGTAATATTAAGTCTCTCAATAAGGACATTCTAAAAAATCCGATCGCGATTCTACTTGATACACAAGGACCGGAGATC
AGAACGGGAGACCTTCAGGTGGATCACCTAGATCTAAAGGTGGGCGAGACGTTCGTCTTTCATATCATTCCCGGGGAAGA
ATCCGAGGAACAATCCGTGTTTGTTAACTATAAGGATATCGTAAAAGATTTAAAAGTGGGCGATCCTGTGACCGTAGACA
ACGGACTCATCAACCTTATCGTAGAAGAGATTAACGACTCGGCGCTCAAGTGTAAGGTTCTAGACGGAGGAAAATTAGGT
TCCAGAAAACACATCAATCTTCCCGGAATTCGAGTGAATCTTCCGTCGATCACGCCGAAGGATCACAAGGATATCCTATT
CGGATTGGAAGAGGACGTGGACTTTATCGCTCTTTCTTTCGTTCGTTCCGCCGAAGACATCAATCAATTGAAACAAATCA
TAGAAGAAAACGAAGGACACGCACAGGTTATCGCAAAGATAGAGGACCAAGAAGCCGTTCGTAACATGAAAGAAATCGTG
GAAGTTTCGGACGGTGTGATGGTCGCGAGAGGGGATTTGGGGGTGGAAGTTCCAATCGAGGAATTGCCGATCCTTCAAAG
GGCGATCATCAAAGAATGTGCCCTCAAAGGGAAAAGAGTCATCGTCGCGACTCATCTTTTGGAATCGATGATTAACAATC
CTTCTCCTACAAGAGCCGAAGTCACCGACGTCGCGAATGCGGTCTATGAAGAGGTGGACGCCATCATGTTATCGGGAGAA
ACCGCCGCCGGTAAGTTTCCGATCCGATGTGTGGAGATGATGGACAAGATAGCACAACGTGTCGAGAAGGCAGGCGGTGT
CGATTACGTTAAGGACAAAATTCCTCAGGATAAAAAGGAACAGATGGCGAGGTCCGCTGCGGAACTTGCGGATTCCCTTA
AATGTCCTGCGATTATCGTCATTACGAGAAGGGGAACTACGGCGCTCAACGTAGCAGGTTTTCATCCGCATTATCCTTTG
ATCTACGCATTTACCAATATGACTACGGTTAGGCGTAAACTCTGGCTCACTCGCGGCGTAATTCCATATCGAATCGATTT
TTCCAAGGATCCTGAAAAAACGATCCGGCTCGCGATCGAAACTCTCAAAAAGGCGGGAAAAATCGAGGATGGGGACCAGG
TTGTGATTTTATCGGATATCATCGCCGGAGAAGATCGAGTGGAGACGATCCAAATCCGCGAAGTAAAAACATATTTTAAT
GCGGAAAAGCTTTGA

Upstream 100 bases:

>100_bases
ATTTGTTCGACTTTTGTATTTCTTTGGAAGCTTTTCTACGGAAAACCATGCCTAAAATCTGAATTGTCCTAAGGTCCGCT
CTCCCGAATAAAGGTACTAC

Downstream 100 bases:

>100_bases
AATTGAAAATCCTTCTGATTCTCAACATGCGAATATCAATCAGTAGTATTTCGCGATCATCAATTTGAATTTCGTAAAAA
CTAAAAAAACGAAATCTGAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 484; Mature: 484

Protein sequence:

>484_residues
MKSEFSVIRKTKIICTIGPATADKKMIQALAEAGMNVARLNMSHGNHDFHRSIIRNIKSLNKDILKNPIAILLDTQGPEI
RTGDLQVDHLDLKVGETFVFHIIPGEESEEQSVFVNYKDIVKDLKVGDPVTVDNGLINLIVEEINDSALKCKVLDGGKLG
SRKHINLPGIRVNLPSITPKDHKDILFGLEEDVDFIALSFVRSAEDINQLKQIIEENEGHAQVIAKIEDQEAVRNMKEIV
EVSDGVMVARGDLGVEVPIEELPILQRAIIKECALKGKRVIVATHLLESMINNPSPTRAEVTDVANAVYEEVDAIMLSGE
TAAGKFPIRCVEMMDKIAQRVEKAGGVDYVKDKIPQDKKEQMARSAAELADSLKCPAIIVITRRGTTALNVAGFHPHYPL
IYAFTNMTTVRRKLWLTRGVIPYRIDFSKDPEKTIRLAIETLKKAGKIEDGDQVVILSDIIAGEDRVETIQIREVKTYFN
AEKL

Sequences:

>Translated_484_residues
MKSEFSVIRKTKIICTIGPATADKKMIQALAEAGMNVARLNMSHGNHDFHRSIIRNIKSLNKDILKNPIAILLDTQGPEI
RTGDLQVDHLDLKVGETFVFHIIPGEESEEQSVFVNYKDIVKDLKVGDPVTVDNGLINLIVEEINDSALKCKVLDGGKLG
SRKHINLPGIRVNLPSITPKDHKDILFGLEEDVDFIALSFVRSAEDINQLKQIIEENEGHAQVIAKIEDQEAVRNMKEIV
EVSDGVMVARGDLGVEVPIEELPILQRAIIKECALKGKRVIVATHLLESMINNPSPTRAEVTDVANAVYEEVDAIMLSGE
TAAGKFPIRCVEMMDKIAQRVEKAGGVDYVKDKIPQDKKEQMARSAAELADSLKCPAIIVITRRGTTALNVAGFHPHYPL
IYAFTNMTTVRRKLWLTRGVIPYRIDFSKDPEKTIRLAIETLKKAGKIEDGDQVVILSDIIAGEDRVETIQIREVKTYFN
AEKL
>Mature_484_residues
MKSEFSVIRKTKIICTIGPATADKKMIQALAEAGMNVARLNMSHGNHDFHRSIIRNIKSLNKDILKNPIAILLDTQGPEI
RTGDLQVDHLDLKVGETFVFHIIPGEESEEQSVFVNYKDIVKDLKVGDPVTVDNGLINLIVEEINDSALKCKVLDGGKLG
SRKHINLPGIRVNLPSITPKDHKDILFGLEEDVDFIALSFVRSAEDINQLKQIIEENEGHAQVIAKIEDQEAVRNMKEIV
EVSDGVMVARGDLGVEVPIEELPILQRAIIKECALKGKRVIVATHLLESMINNPSPTRAEVTDVANAVYEEVDAIMLSGE
TAAGKFPIRCVEMMDKIAQRVEKAGGVDYVKDKIPQDKKEQMARSAAELADSLKCPAIIVITRRGTTALNVAGFHPHYPL
IYAFTNMTTVRRKLWLTRGVIPYRIDFSKDPEKTIRLAIETLKKAGKIEDGDQVVILSDIIAGEDRVETIQIREVKTYFN
AEKL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=490, Percent_Identity=39.3877551020408, Blast_Score=336, Evalue=4e-92,
Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=38.6831275720165, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=38.6831275720165, Blast_Score=335, Evalue=6e-92,
Organism=Homo sapiens, GI10835121, Length=493, Percent_Identity=39.1480730223124, Blast_Score=326, Evalue=4e-89,
Organism=Homo sapiens, GI32967597, Length=493, Percent_Identity=39.1480730223124, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=42.1602787456446, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=42.1602787456446, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=40.2542372881356, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=40.2542372881356, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=39.5348837209302, Blast_Score=166, Evalue=5e-41,
Organism=Escherichia coli, GI1787965, Length=455, Percent_Identity=40.8791208791209, Blast_Score=341, Evalue=8e-95,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=34.7280334728033, Blast_Score=249, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI17544584, Length=480, Percent_Identity=39.5833333333333, Blast_Score=338, Evalue=4e-93,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=41.7355371900826, Blast_Score=328, Evalue=5e-90,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=41.7355371900826, Blast_Score=327, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=41.7355371900826, Blast_Score=327, Evalue=9e-90,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=41.7355371900826, Blast_Score=327, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6319279, Length=489, Percent_Identity=37.6278118609407, Blast_Score=311, Evalue=1e-85,
Organism=Saccharomyces cerevisiae, GI6324923, Length=488, Percent_Identity=35.0409836065574, Blast_Score=293, Evalue=3e-80,
Organism=Drosophila melanogaster, GI24648964, Length=492, Percent_Identity=40.0406504065041, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI28571814, Length=492, Percent_Identity=40.0406504065041, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24648966, Length=410, Percent_Identity=37.5609756097561, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24581235, Length=495, Percent_Identity=34.7474747474747, Blast_Score=276, Evalue=3e-74,
Organism=Drosophila melanogaster, GI24646914, Length=267, Percent_Identity=34.8314606741573, Blast_Score=165, Evalue=5e-41,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 53816; Mature: 53816

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSEFSVIRKTKIICTIGPATADKKMIQALAEAGMNVARLNMSHGNHDFHRSIIRNIKSL
CCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
NKDILKNPIAILLDTQGPEIRTGDLQVDHLDLKVGETFVFHIIPGEESEEQSVFVNYKDI
HHHHHCCCEEEEEECCCCCEEECCEEEEEEEEEECCEEEEEEECCCCCCCCEEEEEHHHH
VKDLKVGDPVTVDNGLINLIVEEINDSALKCKVLDGGKLGSRKHINLPGIRVNLPSITPK
HHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCC
DHKDILFGLEEDVDFIALSFVRSAEDINQLKQIIEENEGHAQVIAKIEDQEAVRNMKEIV
CCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
EVSDGVMVARGDLGVEVPIEELPILQRAIIKECALKGKRVIVATHLLESMINNPSPTRAE
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHH
VTDVANAVYEEVDAIMLSGETAAGKFPIRCVEMMDKIAQRVEKAGGVDYVKDKIPQDKKE
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
QMARSAAELADSLKCPAIIVITRRGTTALNVAGFHPHYPLIYAFTNMTTVRRKLWLTRGV
HHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCC
IPYRIDFSKDPEKTIRLAIETLKKAGKIEDGDQVVILSDIIAGEDRVETIQIREVKTYFN
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHC
AEKL
CCCC
>Mature Secondary Structure
MKSEFSVIRKTKIICTIGPATADKKMIQALAEAGMNVARLNMSHGNHDFHRSIIRNIKSL
CCCHHHHHHEEEEEEEECCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHH
NKDILKNPIAILLDTQGPEIRTGDLQVDHLDLKVGETFVFHIIPGEESEEQSVFVNYKDI
HHHHHCCCEEEEEECCCCCEEECCEEEEEEEEEECCEEEEEEECCCCCCCCEEEEEHHHH
VKDLKVGDPVTVDNGLINLIVEEINDSALKCKVLDGGKLGSRKHINLPGIRVNLPSITPK
HHHHCCCCCEEECCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCC
DHKDILFGLEEDVDFIALSFVRSAEDINQLKQIIEENEGHAQVIAKIEDQEAVRNMKEIV
CCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECHHHHHHHHHHHH
EVSDGVMVARGDLGVEVPIEELPILQRAIIKECALKGKRVIVATHLLESMINNPSPTRAE
HHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCCCHHH
VTDVANAVYEEVDAIMLSGETAAGKFPIRCVEMMDKIAQRVEKAGGVDYVKDKIPQDKKE
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCHHH
QMARSAAELADSLKCPAIIVITRRGTTALNVAGFHPHYPLIYAFTNMTTVRRKLWLTRGV
HHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCC
IPYRIDFSKDPEKTIRLAIETLKKAGKIEDGDQVVILSDIIAGEDRVETIQIREVKTYFN
CEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCEEEEEEEHHHHHHC
AEKL
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]