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The map label for this gene is pbuE [H]

Identifier: 116332632

GI number: 116332632

Start: 241644

End: 242858

Strand: Reverse

Name: pbuE [H]

Synonym: LBJ_4210

Alternate gene names: 116332632

Gene position: 242858-241644 (Counterclockwise)

Preceding gene: 116332637

Following gene: 116332631

Centisome position: 81.02

GC content: 43.13

Gene sequence:

>1215_bases
ATGTCTTCCCGTTCTATCCGTAACATTCATGTCAAATCCGAAACGATGCTGGTCTTTCTATTGGCAGCTGTTCAATTTAC
CCATATTCTCGATTTTGTAATTATGATGCCTCTGGGAACTTATTTTCAGGAGGCGTTTCATATCAATCCGAGAGAATTTT
CCTTTCTCATTTCTGCATATACCTACAGTGCGTTTATTGCCGGAATCGTAGGTGCATTTTTTATCGATCGTTTCGATCGA
AAATCCGCGGCGATTTTTTTATACGCCGGTTTTATTGCAGGAACCACTTTTTGTGCAATCGCGGATTCGTACTTTTTTCT
TTTGATTGCTAGGATTATTTCCGGTGCATTCGGAGGAATTTTAAGTTCCGTCGTCTTTGCAATTGTGGGGGATGTGATTG
CAATGGATCGAAGGGGGAGAGCGACCGGTGCGATCATGGGAGCTTTTTCCGTCGCTTCTGTGATTGGAATTCCCTTGGGA
CTTAAAGTGGCGGAATACTACGGGTGGAATATGTCTTTTGCCGGGATTGTCCTTTTGAGTCTACCGATTTTGGTTCTGAT
GAATTATCATCTTCCGAATATTCCTCCGTTTCAATCCGCGGAAGTCGATCCGATCTGGAATTTTGTTCGGATTCTTACAT
ATAGAAAGTACATAGCTTCTTATATGCTTATTATGTTTGTGATTCTCGGCGGCTTTACTGTAATTCCGTTTATCGCTCCT
TATATGGAAAGAAATGTGGGGATCCTTAAGGACGACATTCCTTGGATTTATTTCTTCGGCGGCCTTGTTACGTTCTTTTC
TTCCAGAATGATCGGAATCGTTTCGGATAAAATCGGAAAACACAAAGTATTCTATATTCTGGTTTCCTTATCTTTCATTC
CGATCTTTATCATGACAAACCTCGGACAAACTTCGCTTGTGAATGTTCTGATTCTCACGACCGTTTTTATGGTAGTCGTA
TCGGGAAGATGGATCCCTGCTTTGGCCTTGATAACATCCGCGACAGAACCGAGTGATCGAGGGCGTTTTATGACGGTGAT
CTCGGCTTTGCAAAATCTCGCTTCCGGACTCGGGGCTACGATTGGAGGAAGTATTCTTGTTGCCGCAGCAAGCCCGACTG
CTCCCTACCAGAATTACGAGGTGGCGGGATATCTGGCGATGGGATTTAATGTAATTGCTTTGGTGTTGATTTCCAAGGTA
AAAACGGTTTCTTGA

Upstream 100 bases:

>100_bases
TATCTTTCATAAGGAATTTTTTAAAAAAGAAATAAAGTCTTCAATCCATTTTTCAAGTTTTTCTATCTACAGAAAAACCC
ACATTTTGCAAAATGATCCC

Downstream 100 bases:

>100_bases
ACCTGTTCTTCCGGAATTAGTGAAATGATTTAGATATTCATGTTTATTTTAGTAGTTGTTTGTGCTGAACAGTGTATCAC
TCTAAAATTGTAGGAACTCC

Product: sugar permease (arabinose polymer)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSSRSIRNIHVKSETMLVFLLAAVQFTHILDFVIMMPLGTYFQEAFHINPREFSFLISAYTYSAFIAGIVGAFFIDRFDR
KSAAIFLYAGFIAGTTFCAIADSYFFLLIARIISGAFGGILSSVVFAIVGDVIAMDRRGRATGAIMGAFSVASVIGIPLG
LKVAEYYGWNMSFAGIVLLSLPILVLMNYHLPNIPPFQSAEVDPIWNFVRILTYRKYIASYMLIMFVILGGFTVIPFIAP
YMERNVGILKDDIPWIYFFGGLVTFFSSRMIGIVSDKIGKHKVFYILVSLSFIPIFIMTNLGQTSLVNVLILTTVFMVVV
SGRWIPALALITSATEPSDRGRFMTVISALQNLASGLGATIGGSILVAAASPTAPYQNYEVAGYLAMGFNVIALVLISKV
KTVS

Sequences:

>Translated_404_residues
MSSRSIRNIHVKSETMLVFLLAAVQFTHILDFVIMMPLGTYFQEAFHINPREFSFLISAYTYSAFIAGIVGAFFIDRFDR
KSAAIFLYAGFIAGTTFCAIADSYFFLLIARIISGAFGGILSSVVFAIVGDVIAMDRRGRATGAIMGAFSVASVIGIPLG
LKVAEYYGWNMSFAGIVLLSLPILVLMNYHLPNIPPFQSAEVDPIWNFVRILTYRKYIASYMLIMFVILGGFTVIPFIAP
YMERNVGILKDDIPWIYFFGGLVTFFSSRMIGIVSDKIGKHKVFYILVSLSFIPIFIMTNLGQTSLVNVLILTTVFMVVV
SGRWIPALALITSATEPSDRGRFMTVISALQNLASGLGATIGGSILVAAASPTAPYQNYEVAGYLAMGFNVIALVLISKV
KTVS
>Mature_403_residues
SSRSIRNIHVKSETMLVFLLAAVQFTHILDFVIMMPLGTYFQEAFHINPREFSFLISAYTYSAFIAGIVGAFFIDRFDRK
SAAIFLYAGFIAGTTFCAIADSYFFLLIARIISGAFGGILSSVVFAIVGDVIAMDRRGRATGAIMGAFSVASVIGIPLGL
KVAEYYGWNMSFAGIVLLSLPILVLMNYHLPNIPPFQSAEVDPIWNFVRILTYRKYIASYMLIMFVILGGFTVIPFIAPY
MERNVGILKDDIPWIYFFGGLVTFFSSRMIGIVSDKIGKHKVFYILVSLSFIPIFIMTNLGQTSLVNVLILTTVFMVVVS
GRWIPALALITSATEPSDRGRFMTVISALQNLASGLGATIGGSILVAAASPTAPYQNYEVAGYLAMGFNVIALVLISKVK
TVS

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44255; Mature: 44124

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSRSIRNIHVKSETMLVFLLAAVQFTHILDFVIMMPLGTYFQEAFHINPREFSFLISAY
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TYSAFIAGIVGAFFIDRFDRKSAAIFLYAGFIAGTTFCAIADSYFFLLIARIISGAFGGI
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSVVFAIVGDVIAMDRRGRATGAIMGAFSVASVIGIPLGLKVAEYYGWNMSFAGIVLLS
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHH
LPILVLMNYHLPNIPPFQSAEVDPIWNFVRILTYRKYIASYMLIMFVILGGFTVIPFIAP
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YMERNVGILKDDIPWIYFFGGLVTFFSSRMIGIVSDKIGKHKVFYILVSLSFIPIFIMTN
HHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
LGQTSLVNVLILTTVFMVVVSGRWIPALALITSATEPSDRGRFMTVISALQNLASGLGAT
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHH
IGGSILVAAASPTAPYQNYEVAGYLAMGFNVIALVLISKVKTVS
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]