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The map label for this gene is hetN [H]

Identifier: 116332477

GI number: 116332477

Start: 35409

End: 36212

Strand: Direct

Name: hetN [H]

Synonym: LBJ_4033

Alternate gene names: 116332477

Gene position: 35409-36212 (Clockwise)

Preceding gene: 116332475

Following gene: 116332478

Centisome position: 11.81

GC content: 43.78

Gene sequence:

>804_bases
ATGAATCCTTCGTTCTGGACAGGCAAAACCATCGTAATGACCGGAGGTTCTTCCGGTATTGGAGAAGCCGTCCTCGGCGC
TCTTTCTCGTATCCGTTGTAATCTTCTCAATCTTTCCAGAACCCAGCCTGAGCTTTTAAAACGAAAGAATAACCTTCCTG
CAAATCTGATTCATATTCCGACCGACTTGAGTTCGGAAAAGGAAATCGATAAGGCAGTCAAAAAAATTTCCAAAGACTTT
CGGGGTATAGACGTTCTTTTTGCAAATGCGGGAGTGACTACACATTCCCGTTTTGACGGAACGAGAATCGAAACCTTTCG
CAGGACGTTCGATATCAATTTTTTCGGTCCTATCTATCTTATCCAAAGATTGCTTCCGCAGATTAAACTGACCAAAGGTT
CCGTGGTCGCGACCTCCACCGTAAGTGGTCTTTATGGAATTCCGGGAAGAAGCGCGTATTCTTCCTCTAAGTCCGCACTT
CACGCAGCTTTGGAGGCGGCAAGAATAGAACTTTCGGAAGAAGGGTTGTCGTTTATCATTTTTTGCCCTCCTTATACAAA
GACGAAACTTCGAACATCCGGTTTGGACGGAGACGGTAACGTTTTGAAAGAAGATCACTATTCCGGTAAGAGGATCAAAT
CTCCACAAGAGGTTGCGTCTAAGATGATCGATTCGGTGGAGGATCCGGAATCGAGACTAGTCATCATGGATAGTTCCGGA
TTCTTTTTGAAATGGTTGCGTAATATCGCTCCCGCTTTTTTGGAGCGAACTTTATTCAAAAAACTTTATAAAGATTTTCA
CTAA

Upstream 100 bases:

>100_bases
GGGAAGTGTGTGCTATGTGGGAGGATGCAACGGAATGTTCTTCTTTTCCCTTGAAAAAATTCTTGAATCCCTCTTTCTTA
TGTCGAGAATGCTCTGTAAC

Downstream 100 bases:

>100_bases
AAGGAAAGCATGGAAACTAGAAGCGTTGTAAAAGAATTTCAGTTCGACTATCCTCTGATGAGAGTTTGGAATGCGGTCAC
CGTAAACGAAGAACTGGTCC

Product: Short chain dehydrogenase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MNPSFWTGKTIVMTGGSSGIGEAVLGALSRIRCNLLNLSRTQPELLKRKNNLPANLIHIPTDLSSEKEIDKAVKKISKDF
RGIDVLFANAGVTTHSRFDGTRIETFRRTFDINFFGPIYLIQRLLPQIKLTKGSVVATSTVSGLYGIPGRSAYSSSKSAL
HAALEAARIELSEEGLSFIIFCPPYTKTKLRTSGLDGDGNVLKEDHYSGKRIKSPQEVASKMIDSVEDPESRLVIMDSSG
FFLKWLRNIAPAFLERTLFKKLYKDFH

Sequences:

>Translated_267_residues
MNPSFWTGKTIVMTGGSSGIGEAVLGALSRIRCNLLNLSRTQPELLKRKNNLPANLIHIPTDLSSEKEIDKAVKKISKDF
RGIDVLFANAGVTTHSRFDGTRIETFRRTFDINFFGPIYLIQRLLPQIKLTKGSVVATSTVSGLYGIPGRSAYSSSKSAL
HAALEAARIELSEEGLSFIIFCPPYTKTKLRTSGLDGDGNVLKEDHYSGKRIKSPQEVASKMIDSVEDPESRLVIMDSSG
FFLKWLRNIAPAFLERTLFKKLYKDFH
>Mature_267_residues
MNPSFWTGKTIVMTGGSSGIGEAVLGALSRIRCNLLNLSRTQPELLKRKNNLPANLIHIPTDLSSEKEIDKAVKKISKDF
RGIDVLFANAGVTTHSRFDGTRIETFRRTFDINFFGPIYLIQRLLPQIKLTKGSVVATSTVSGLYGIPGRSAYSSSKSAL
HAALEAARIELSEEGLSFIIFCPPYTKTKLRTSGLDGDGNVLKEDHYSGKRIKSPQEVASKMIDSVEDPESRLVIMDSSG
FFLKWLRNIAPAFLERTLFKKLYKDFH

Specific function: May be involved in repressing heterocyst differentiation and may be essential for preventing all vegetative cells from differentiating [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI20149619, Length=261, Percent_Identity=26.8199233716475, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI7706318, Length=201, Percent_Identity=26.3681592039801, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI4503817, Length=195, Percent_Identity=26.6666666666667, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI40807363, Length=253, Percent_Identity=23.7154150197628, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI142976729, Length=267, Percent_Identity=23.2209737827715, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI32455239, Length=174, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI5031765, Length=174, Percent_Identity=26.4367816091954, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI4758504, Length=239, Percent_Identity=28.0334728033473, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI7657478, Length=181, Percent_Identity=27.6243093922652, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI210032110, Length=260, Percent_Identity=24.2307692307692, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1786701, Length=184, Percent_Identity=32.0652173913043, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71997402, Length=264, Percent_Identity=25, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17570069, Length=267, Percent_Identity=28.0898876404494, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17567345, Length=173, Percent_Identity=26.0115606936416, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=196, Percent_Identity=30.1020408163265, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17508895, Length=193, Percent_Identity=26.9430051813472, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538486, Length=210, Percent_Identity=27.1428571428571, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322779, Length=230, Percent_Identity=30.4347826086957, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322226, Length=190, Percent_Identity=27.8947368421053, Blast_Score=69, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6322227, Length=198, Percent_Identity=26.7676767676768, Blast_Score=62, Evalue=6e-11,
Organism=Drosophila melanogaster, GI21358495, Length=266, Percent_Identity=26.6917293233083, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI21357041, Length=212, Percent_Identity=31.1320754716981, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28571526, Length=195, Percent_Identity=28.2051282051282, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24644339, Length=196, Percent_Identity=27.0408163265306, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI23397609, Length=187, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24580925, Length=188, Percent_Identity=27.1276595744681, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21356301, Length=197, Percent_Identity=25.3807106598985, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24643142, Length=190, Percent_Identity=27.3684210526316, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI28571387, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281364494, Length=200, Percent_Identity=25.5, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 29660; Mature: 29660

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSFWTGKTIVMTGGSSGIGEAVLGALSRIRCNLLNLSRTQPELLKRKNNLPANLIHIP
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEECC
TDLSSEKEIDKAVKKISKDFRGIDVLFANAGVTTHSRFDGTRIETFRRTFDINFFGPIYL
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
IQRLLPQIKLTKGSVVATSTVSGLYGIPGRSAYSSSKSALHAALEAARIELSEEGLSFII
HHHHHHHHEECCCCEEEEHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEE
FCPPYTKTKLRTSGLDGDGNVLKEDHYSGKRIKSPQEVASKMIDSVEDPESRLVIMDSSG
ECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
FFLKWLRNIAPAFLERTLFKKLYKDFH
HHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPSFWTGKTIVMTGGSSGIGEAVLGALSRIRCNLLNLSRTQPELLKRKNNLPANLIHIP
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCEEEECC
TDLSSEKEIDKAVKKISKDFRGIDVLFANAGVTTHSRFDGTRIETFRRTFDINFFGPIYL
CCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHH
IQRLLPQIKLTKGSVVATSTVSGLYGIPGRSAYSSSKSALHAALEAARIELSEEGLSFII
HHHHHHHHEECCCCEEEEHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEE
FCPPYTKTKLRTSGLDGDGNVLKEDHYSGKRIKSPQEVASKMIDSVEDPESRLVIMDSSG
ECCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
FFLKWLRNIAPAFLERTLFKKLYKDFH
HHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8157596; 11759840 [H]