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The map label for this gene is dapF

Identifier: 116329548

GI number: 116329548

Start: 3600961

End: 3601806

Strand: Direct

Name: dapF

Synonym: LBL_3025

Alternate gene names: 116329548

Gene position: 3600961-3601806 (Clockwise)

Preceding gene: 116329546

Following gene: 116329550

Centisome position: 99.63

GC content: 42.55

Gene sequence:

>846_bases
GTGGCCGCGCTTAAGTTTACAAAAATGGAAGGAATTGGTAACGACTATATTTATATCGATTCTACTCAGGCTAATATTCG
CCTGACTCCGGAGCAGATTCAAAAAATATCCAATCGTAATTTTGGAATTGGGAGTGACGGGGTTATCTTTATTCGTAATT
CGAAACAAGGCGATTTTATGATGGACATGTACAATTCGGATGGAAGTTCCTCTGAGATGTGCGGAAATGGAATTCGTTGT
GTCGCCAAATACATCTACGATCACGGTTTAACGAATTCTAAAAATCCAAAAATCGAAACCGGCGCAGGTGTTTTAGAAGT
GGATCTCAAGATTGGATCGGGTAATAAGGTCGATTTTGTCAGCGTTAACATGGGAAAACCGATTCTTGTCCCGTCCAAAA
TTCCTGTCAATTGGAAGGATGAAGAAGCGATCATCGATCAAACATTTGAGATCGCGGGAAAAAATTTAAAATTTACCGCG
GTAAGTATGGGAAATCCACATTGTGTAATTTTTGTGGATGATTGTGATCAATTTCCCGTGACTGGAATTGGGCCTTTGAT
CGAACGGCATCCTATTTTTCCGAAACGAATAAATGTGGAGTTTGTTACGGTTCGCGGAAAGGATCATTTCTATCAAAGAA
CCTGGGAAAGAGGCGCGGGAGAAACACTTGCGTGTGGAACCGGAGCCTGTGCAGTGACGGTTGCCGGAAATTTAACGGGA
AAATCGGGAAAGGAAGTGAAGATCGATTTGAGAGGTGGGACTCTTAGAATCCAATGGCAGGAATCCGGAAACGTTTTGAT
GACCGGGCCTGCAAAAGAGATTTTCTCGGGGGAAATAGAAGTTTAG

Upstream 100 bases:

>100_bases
CGAAAAAGCCAGAAAAAAGTACATGACTTTTTCTTGTTTTTGAATTCCCTCGGGTAACAGTCTAAGAAAATATAGGATTC
AATTTATCGTAGGAGTTCTT

Downstream 100 bases:

>100_bases
AATTTTCTAAACTACTTGGGTTCTTTGCAGATTTTCCATTCTCACTCGAACTTCGTCCGAAAGTTTTTTTAAATTATCTT
CCAGTTTTCCCTCGAGATAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MAALKFTKMEGIGNDYIYIDSTQANIRLTPEQIQKISNRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRC
VAKYIYDHGLTNSKNPKIETGAGVLEVDLKIGSGNKVDFVSVNMGKPILVPSKIPVNWKDEEAIIDQTFEIAGKNLKFTA
VSMGNPHCVIFVDDCDQFPVTGIGPLIERHPIFPKRINVEFVTVRGKDHFYQRTWERGAGETLACGTGACAVTVAGNLTG
KSGKEVKIDLRGGTLRIQWQESGNVLMTGPAKEIFSGEIEV

Sequences:

>Translated_281_residues
MAALKFTKMEGIGNDYIYIDSTQANIRLTPEQIQKISNRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRC
VAKYIYDHGLTNSKNPKIETGAGVLEVDLKIGSGNKVDFVSVNMGKPILVPSKIPVNWKDEEAIIDQTFEIAGKNLKFTA
VSMGNPHCVIFVDDCDQFPVTGIGPLIERHPIFPKRINVEFVTVRGKDHFYQRTWERGAGETLACGTGACAVTVAGNLTG
KSGKEVKIDLRGGTLRIQWQESGNVLMTGPAKEIFSGEIEV
>Mature_280_residues
AALKFTKMEGIGNDYIYIDSTQANIRLTPEQIQKISNRNFGIGSDGVIFIRNSKQGDFMMDMYNSDGSSSEMCGNGIRCV
AKYIYDHGLTNSKNPKIETGAGVLEVDLKIGSGNKVDFVSVNMGKPILVPSKIPVNWKDEEAIIDQTFEIAGKNLKFTAV
SMGNPHCVIFVDDCDQFPVTGIGPLIERHPIFPKRINVEFVTVRGKDHFYQRTWERGAGETLACGTGACAVTVAGNLTGK
SGKEVKIDLRGGTLRIQWQESGNVLMTGPAKEIFSGEIEV

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=281, Percent_Identity=38.0782918149466, Blast_Score=213, Evalue=1e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_LEPBJ (Q04W97)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799581.1
- ProteinModelPortal:   Q04W97
- SMR:   Q04W97
- STRING:   Q04W97
- GeneID:   4411183
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0071
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   Q04W97
- ProtClustDB:   PRK00450
- BioCyc:   LBOR355277:LBJ_0071-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30737; Mature: 30605

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 74-74 ACT_SITE 225-225

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALKFTKMEGIGNDYIYIDSTQANIRLTPEQIQKISNRNFGIGSDGVIFIRNSKQGDFM
CCCEEEEEECCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCCCEE
MDMYNSDGSSSEMCGNGIRCVAKYIYDHGLTNSKNPKIETGAGVLEVDLKIGSGNKVDFV
EEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCEEEEE
SVNMGKPILVPSKIPVNWKDEEAIIDQTFEIAGKNLKFTAVSMGNPHCVIFVDDCDQFPV
EECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCC
TGIGPLIERHPIFPKRINVEFVTVRGKDHFYQRTWERGAGETLACGTGACAVTVAGNLTG
CCCCHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCC
KSGKEVKIDLRGGTLRIQWQESGNVLMTGPAKEIFSGEIEV
CCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHCCCCCCC
>Mature Secondary Structure 
AALKFTKMEGIGNDYIYIDSTQANIRLTPEQIQKISNRNFGIGSDGVIFIRNSKQGDFM
CCEEEEEECCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCCCCCCEEEEECCCCCCEE
MDMYNSDGSSSEMCGNGIRCVAKYIYDHGLTNSKNPKIETGAGVLEVDLKIGSGNKVDFV
EEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCCCCCEECCCCEEEEEEEECCCCEEEEE
SVNMGKPILVPSKIPVNWKDEEAIIDQTFEIAGKNLKFTAVSMGNPHCVIFVDDCDQFPV
EECCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCCCCCC
TGIGPLIERHPIFPKRINVEFVTVRGKDHFYQRTWERGAGETLACGTGACAVTVAGNLTG
CCCCHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHCCCCCEEEECCCCEEEEEEECCCC
KSGKEVKIDLRGGTLRIQWQESGNVLMTGPAKEIFSGEIEV
CCCCEEEEEECCCEEEEEECCCCCEEEECCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA