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The map label for this gene is tilS

Identifier: 116329484

GI number: 116329484

Start: 3516651

End: 3517928

Strand: Direct

Name: tilS

Synonym: LBL_2955

Alternate gene names: 116329484

Gene position: 3516651-3517928 (Clockwise)

Preceding gene: 116329481

Following gene: 116329485

Centisome position: 97.29

GC content: 36.07

Gene sequence:

>1278_bases
ATGAGAGATAAGATCTCCGAATCCACACGAAATATTTTTGATACGGTCTGGAAAAGAATCTTTCCTTTTCACGAGATGAT
ACTATCTCGTCCCGCTGTTCTTTCCTATTCCGGCGGGAAAGATTCTTCCCTTTTGCTTCATTTTTATTTTTGGCTTTGGG
CCGAGAAAAAAATTCCAGTACCTTGCATTTATCACTTAGATCATTCGATTCGATTCAATTTGGAACAGGAAAAAAAAATC
CTCGATTATACGGAGAGCACGTTTCCATTCCCGAATTTATTCAAAAAAAAAAATATTCCAGCTTTATCTCAAAAGTTAGG
CAAAACACTCGAAGAAACCGGAAGAGCTTTCAGATACAAGGATCTGGAAAAAATTTCGAATCAATACGAAGGTTATATTG
TTACGGGCCATCATTCTACAGATTATCTAGAAACCGTTTTTCTTAATCTGATTCGAGGAGGTGGTTGGAATTCTTTACGA
ACCTTAGGTTGGTATGAAAAAAATCGGTTTAGACCTCTTTTTGCTTTTACTGAAGACGAAATCAAAACGATTTCGCAATC
CGAATCTTGGCCCATCTTCGAAGACGAATCTAATCAAAGTAACGAATATCTTAGAAATCGAATTCGAAATTATATCTTAC
CTTTACTGTTACAAGAAGGAGCGGACCCAGATCGAATTTATAAAAACTTTCATCGAATGGAAAAACCTACTTCTAAAATT
CTTCTTAAAGAAGTTTCATCTCATAAAACTCCTTCTTTTTTGAAAATTGATGTATGGGTCCTGAACGATCTTTCCGAAAG
AGAAAGAAAATTTTTTATAGATCGTTATTTACGGTCTTTAAATCTACATCCGACGACTCGGAACTTTTTTCAAGATCTTA
TGGATTGTTTGAAAAAGATGAATTCTTTCGGTATTGAGAACAAAGAAGCGTGGTTTTGGAAATCGTCCTCTTACGACCTT
TACGTGATCCCTAAAAACTCACTCTGTCTAAAAAAATTTAAATTAGAATCGAAAAACATGATACTTAAATGGAATGGAAG
CCAAAAAAAAATTTCTCCTGGATTCATTCCAGGTTTATGCTCTCCCGGCGCCAAAATTCGCAAAAATGGGATGAGTATTG
AAATTTCCGAAATCTTGCGACAAAAAGAGATTCCGGTTCCAGTTAGAAAAATGCTACCCATACTTTATAGGGAAGGGAAA
GTTGATGTGATCTGTCTGAGTCTTTGGGATCCGAGATTAGGGGACATTGTAGCGGACAGGAGTCGAAATTTTATCTGA

Upstream 100 bases:

>100_bases
TCTCTAAAATTCACAAATTAATCGAAAATCAAAAGCGGGTGTACAATATTACTCTTGCAGCAATCCGAAGAAGCCGTTAT
ACCTGCATTAACCAGTTCAG

Downstream 100 bases:

>100_bases
TTTTCAGGAGCTCGGAATATGAACGAGGATCCTCAAAAAAAAGACGAACCATTTTTTAAGGACGTCGAGTTCAAAGCATC
CTACGGAGAGGCGAATCAAA

Product: PP family ATPase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRDKISESTRNIFDTVWKRIFPFHEMILSRPAVLSYSGGKDSSLLLHFYFWLWAEKKIPVPCIYHLDHSIRFNLEQEKKI
LDYTESTFPFPNLFKKKNIPALSQKLGKTLEETGRAFRYKDLEKISNQYEGYIVTGHHSTDYLETVFLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFTEDEIKTISQSESWPIFEDESNQSNEYLRNRIRNYILPLLLQEGADPDRIYKNFHRMEKPTSKI
LLKEVSSHKTPSFLKIDVWVLNDLSERERKFFIDRYLRSLNLHPTTRNFFQDLMDCLKKMNSFGIENKEAWFWKSSSYDL
YVIPKNSLCLKKFKLESKNMILKWNGSQKKISPGFIPGLCSPGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILYREGK
VDVICLSLWDPRLGDIVADRSRNFI

Sequences:

>Translated_425_residues
MRDKISESTRNIFDTVWKRIFPFHEMILSRPAVLSYSGGKDSSLLLHFYFWLWAEKKIPVPCIYHLDHSIRFNLEQEKKI
LDYTESTFPFPNLFKKKNIPALSQKLGKTLEETGRAFRYKDLEKISNQYEGYIVTGHHSTDYLETVFLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFTEDEIKTISQSESWPIFEDESNQSNEYLRNRIRNYILPLLLQEGADPDRIYKNFHRMEKPTSKI
LLKEVSSHKTPSFLKIDVWVLNDLSERERKFFIDRYLRSLNLHPTTRNFFQDLMDCLKKMNSFGIENKEAWFWKSSSYDL
YVIPKNSLCLKKFKLESKNMILKWNGSQKKISPGFIPGLCSPGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILYREGK
VDVICLSLWDPRLGDIVADRSRNFI
>Mature_425_residues
MRDKISESTRNIFDTVWKRIFPFHEMILSRPAVLSYSGGKDSSLLLHFYFWLWAEKKIPVPCIYHLDHSIRFNLEQEKKI
LDYTESTFPFPNLFKKKNIPALSQKLGKTLEETGRAFRYKDLEKISNQYEGYIVTGHHSTDYLETVFLNLIRGGGWNSLR
TLGWYEKNRFRPLFAFTEDEIKTISQSESWPIFEDESNQSNEYLRNRIRNYILPLLLQEGADPDRIYKNFHRMEKPTSKI
LLKEVSSHKTPSFLKIDVWVLNDLSERERKFFIDRYLRSLNLHPTTRNFFQDLMDCLKKMNSFGIENKEAWFWKSSSYDL
YVIPKNSLCLKKFKLESKNMILKWNGSQKKISPGFIPGLCSPGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILYREGK
VDVICLSLWDPRLGDIVADRSRNFI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=201, Percent_Identity=28.8557213930348, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_LEPBJ (Q04W48)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799630.1
- ProteinModelPortal:   Q04W48
- STRING:   Q04W48
- GeneID:   4410540
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0128
- eggNOG:   COG0037
- HOGENOM:   HBG110208
- OMA:   RIAPFHE
- ProtClustDB:   CLSK573376
- BioCyc:   LBOR355277:LBJ_0128-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 50239; Mature: 50239

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDKISESTRNIFDTVWKRIFPFHEMILSRPAVLSYSGGKDSSLLLHFYFWLWAEKKIPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
PCIYHLDHSIRFNLEQEKKILDYTESTFPFPNLFKKKNIPALSQKLGKTLEETGRAFRYK
CEEEECCCCEEECCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
DLEKISNQYEGYIVTGHHSTDYLETVFLNLIRGGGWNSLRTLGWYEKNRFRPLFAFTEDE
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCCEEEEECHHH
IKTISQSESWPIFEDESNQSNEYLRNRIRNYILPLLLQEGADPDRIYKNFHRMEKPTSKI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
LLKEVSSHKTPSFLKIDVWVLNDLSERERKFFIDRYLRSLNLHPTTRNFFQDLMDCLKKM
HHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NSFGIENKEAWFWKSSSYDLYVIPKNSLCLKKFKLESKNMILKWNGSQKKISPGFIPGLC
HHCCCCCCCEEEEECCCCEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
SPGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILYREGKVDVICLSLWDPRLGDIVADR
CCCHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCC
SRNFI
CCCCC
>Mature Secondary Structure
MRDKISESTRNIFDTVWKRIFPFHEMILSRPAVLSYSGGKDSSLLLHFYFWLWAEKKIPV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCC
PCIYHLDHSIRFNLEQEKKILDYTESTFPFPNLFKKKNIPALSQKLGKTLEETGRAFRYK
CEEEECCCCEEECCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCCHHHHH
DLEKISNQYEGYIVTGHHSTDYLETVFLNLIRGGGWNSLRTLGWYEKNRFRPLFAFTEDE
HHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHCCCCCEEEEECHHH
IKTISQSESWPIFEDESNQSNEYLRNRIRNYILPLLLQEGADPDRIYKNFHRMEKPTSKI
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHH
LLKEVSSHKTPSFLKIDVWVLNDLSERERKFFIDRYLRSLNLHPTTRNFFQDLMDCLKKM
HHHHHHCCCCCCEEEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
NSFGIENKEAWFWKSSSYDLYVIPKNSLCLKKFKLESKNMILKWNGSQKKISPGFIPGLC
HHCCCCCCCEEEEECCCCEEEEECCCCHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCC
SPGAKIRKNGMSIEISEILRQKEIPVPVRKMLPILYREGKVDVICLSLWDPRLGDIVADR
CCCHHHHHCCCCEEHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHCC
SRNFI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA