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The map label for this gene is mazG [H]

Identifier: 116329403

GI number: 116329403

Start: 3421365

End: 3422255

Strand: Direct

Name: mazG [H]

Synonym: LBL_2868

Alternate gene names: 116329403

Gene position: 3421365-3422255 (Clockwise)

Preceding gene: 116329400

Following gene: 116329404

Centisome position: 94.66

GC content: 38.16

Gene sequence:

>891_bases
TTGAAAAAGAGTGCAGATTCAGCTTGGAAATTAAAAGTTGGAACAAATCTCCCGTCGTTTTTGGAGGGAACTCATATGCA
GGCAAAATCACTCGCAGAGGAAATTGGTAAACTTCAAGACATCACAGCCACTCTTCGAGGAGAAAACGGATGTCCTTGGG
ATAAGGAACAGGACCATCAAACTTTGATTCCTTATCTCATTGAAGAATCCCAAGAAGTCATCGAAGCAATCTTAAAAAAC
GACGATGAACTTCTCAAAGAAGAATTAGGTGACCTATTATTTCAAGTTGTGTTTCATGCAAGACTTGCTGAAGAAAGACA
TTCATTCGATTTAGGAGACGTTGCAAAAAGCGTTTCCGATAAACTCATCTTCAGACATCCTCACGTTTTTAGACCGGAAG
AACTCACCCTTTCTTCTTCTCAGGAAGTTTTGGAAAATTGGGAGAAAATCAAGGATAAAGAAAAGAAGAAATCAAACGAT
TCATCCATTTTTTCGAACATCCCGGAGAATTTCTCTTCCCTTTTAAAAGCGGAAAAATATCAAAAGAAAGCCGCCAAAGT
CGGATTCGATTGGGAGAACATTTCAGATGTGCAAGGAAAAGTTAAAGAAGAAATGGAAGAATTCTTAATCGAATTCGGTC
GTGTCAAAGCGAACGGAAGCAATCAGGTTCGTATCGAAGAAGAATTCGGAGATTTATTATTCAGTTTAGTAAATCTAGGA
AGGCATCTTGGAATTTCCTCCGAATCCGCACTCACAAGGACGAATGCGAAATTTAAGAAAAGATTTCAATACATAGAACA
AACCTTAAAAAACCGAAATAAAACCCCGAAAGAATCCGATTTAAAAGAAATGGATCGGCTTTGGAATGAGGCAAAAGAAT
CGGAAACATGA

Upstream 100 bases:

>100_bases
TAAGACTTTTTGAACGTATCATAAAGGACCTATAAAGTATTTTTTTGAACTATACTTCAACTTTACTGCTCCCGTCAATC
CAATCCCGATTTCAAAACAA

Downstream 100 bases:

>100_bases
ACGGACCGGATTCACTCGAAAAAAGAATCGAACGAACCGAAACTTTGATTTCTATCCTCTCCAAAGAGTTTTTTTTGAAG
TTAAAATCGGATTCGGAAGA

Product: nucleoside triphosphate pyrophosphohydrolase

Products: NA

Alternate protein names: NTP-PPase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKKSADSAWKLKVGTNLPSFLEGTHMQAKSLAEEIGKLQDITATLRGENGCPWDKEQDHQTLIPYLIEESQEVIEAILKN
DDELLKEELGDLLFQVVFHARLAEERHSFDLGDVAKSVSDKLIFRHPHVFRPEELTLSSSQEVLENWEKIKDKEKKKSND
SSIFSNIPENFSSLLKAEKYQKKAAKVGFDWENISDVQGKVKEEMEEFLIEFGRVKANGSNQVRIEEEFGDLLFSLVNLG
RHLGISSESALTRTNAKFKKRFQYIEQTLKNRNKTPKESDLKEMDRLWNEAKESET

Sequences:

>Translated_296_residues
MKKSADSAWKLKVGTNLPSFLEGTHMQAKSLAEEIGKLQDITATLRGENGCPWDKEQDHQTLIPYLIEESQEVIEAILKN
DDELLKEELGDLLFQVVFHARLAEERHSFDLGDVAKSVSDKLIFRHPHVFRPEELTLSSSQEVLENWEKIKDKEKKKSND
SSIFSNIPENFSSLLKAEKYQKKAAKVGFDWENISDVQGKVKEEMEEFLIEFGRVKANGSNQVRIEEEFGDLLFSLVNLG
RHLGISSESALTRTNAKFKKRFQYIEQTLKNRNKTPKESDLKEMDRLWNEAKESET
>Mature_296_residues
MKKSADSAWKLKVGTNLPSFLEGTHMQAKSLAEEIGKLQDITATLRGENGCPWDKEQDHQTLIPYLIEESQEVIEAILKN
DDELLKEELGDLLFQVVFHARLAEERHSFDLGDVAKSVSDKLIFRHPHVFRPEELTLSSSQEVLENWEKIKDKEKKKSND
SSIFSNIPENFSSLLKAEKYQKKAAKVGFDWENISDVQGKVKEEMEEFLIEFGRVKANGSNQVRIEEEFGDLLFSLVNLG
RHLGISSESALTRTNAKFKKRFQYIEQTLKNRNKTPKESDLKEMDRLWNEAKESET

Specific function: Involved in the regulation of bacterial cell survival under conditions of nutritional stress. Regulates the MazEF toxin- antitoxin (TA) module that mediates programmed cell death (PCD). This is achieved by lowering the cellular concentration of (p)ppGpp p

COG id: COG1694

COG function: function code R; Predicted pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nucleoside triphosphate pyrophosphohydrolase family [H]

Homologues:

Organism=Escherichia coli, GI1789144, Length=263, Percent_Identity=43.7262357414449, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004518
- InterPro:   IPR011551 [H]

Pfam domain/function: PF03819 MazG [H]

EC number: =3.6.1.8 [H]

Molecular weight: Translated: 34062; Mature: 34062

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSADSAWKLKVGTNLPSFLEGTHMQAKSLAEEIGKLQDITATLRGENGCPWDKEQDHQ
CCCCCCCCEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TLIPYLIEESQEVIEAILKNDDELLKEELGDLLFQVVFHARLAEERHSFDLGDVAKSVSD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KLIFRHPHVFRPEELTLSSSQEVLENWEKIKDKEKKKSNDSSIFSNIPENFSSLLKAEKY
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHH
QKKAAKVGFDWENISDVQGKVKEEMEEFLIEFGRVKANGSNQVRIEEEFGDLLFSLVNLG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
RHLGISSESALTRTNAKFKKRFQYIEQTLKNRNKTPKESDLKEMDRLWNEAKESET
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKSADSAWKLKVGTNLPSFLEGTHMQAKSLAEEIGKLQDITATLRGENGCPWDKEQDHQ
CCCCCCCCEEEEECCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
TLIPYLIEESQEVIEAILKNDDELLKEELGDLLFQVVFHARLAEERHSFDLGDVAKSVSD
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
KLIFRHPHVFRPEELTLSSSQEVLENWEKIKDKEKKKSNDSSIFSNIPENFSSLLKAEKY
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHCHHHHHHHHHHHHH
QKKAAKVGFDWENISDVQGKVKEEMEEFLIEFGRVKANGSNQVRIEEEFGDLLFSLVNLG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHH
RHLGISSESALTRTNAKFKKRFQYIEQTLKNRNKTPKESDLKEMDRLWNEAKESET
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]