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The map label for this gene is htrB [H]

Identifier: 116329392

GI number: 116329392

Start: 3401011

End: 3401913

Strand: Direct

Name: htrB [H]

Synonym: LBL_2849

Alternate gene names: 116329392

Gene position: 3401011-3401913 (Clockwise)

Preceding gene: 116329387

Following gene: 116329395

Centisome position: 94.09

GC content: 40.86

Gene sequence:

>903_bases
TTGATTCGTTATATACCGTCATTTATCTTTGTTTATTTATTCTATCTTCCGTTTCGAATCCTACCATATCGTTTTTGTCT
TTTGTATGGAAGGTTCCTAGTTTTTCTTCTTTATCCGTTTGCAAAAAAACACAGAAAGATCGCCTACGAAAATATTACTC
ACGCGTTCCCCGAATATACGGAAACACAAAAGAAAGAACTCGTTTGGAAGAGCATTCTTCACATTGGAAATCTTGTCGCG
GGCACTTTGTTCGCTCCTCGTTTGAATCAAAAATGGATGGATCGTTATCTCGTTTATGATCCCGAATCTTTAGCGATCGA
AAAGAAAACGAACGAAGAAGGCATCGGAGTCGTTTTGATCTCGGGTCATTTCGGAACTTGGGAAATTTTAGTTCAATTTA
TGGGAATTCGTATGAAAGGCGGAGGGATTTATAAAAAAGTAAGAAATCCGTTCGTGGATAAATTGATCTACAAACTCAGA
ACGAAAAACGGAATCAAACTCGTTTCTACGGAAGAATCCAGTCAAGTAACCAAGATGCTAAAGCAAGGTTATTGGGTAGG
ATTCGGTTCAGACCAAAACGCCGGCAAAGTCGGTATTTTTGTCAACTTTTTCAATCGTCCCGCATCCACTTATCAAGGTC
CCGCTTTGATGGCTTATCTCACAGGAGCAAAAATGCTTTTGTATTCGGTTCTCTGTGGTGAGAATGGAAAGGTAATTGTT
CGAGTCAAAGACCTAGGTTGCGTGGACAAAAAAGCGTTTCCGGATCGTGAAACGGCCATTCGTCATTACACAGAGGTCTG
GACAAAAGCCTTAGAAGAAGAAGTAAAGCTTTATCCTGAACAATACTTCTGGGTCCATCGGCGCTGGAGAACGAAACCGG
GAGATTTCCCGGGTCAAATTTGA

Upstream 100 bases:

>100_bases
ACGAAATCACTTCTTCTATCTTCTAAACAGTCGATCTTTTGCTTGTCAATCCAGACAGTAATCGTTCCTTTTGTATTAAA
AGAATGATCTCGGAGTCCTC

Downstream 100 bases:

>100_bases
GTTATCATAGTTAGCAATGCACTGCCTCAAATACTAACATAACGTGAGTTCGATAAGTTTGTAATAAAAAAATCAAACCT
TCGATTGCGAGAAGGTTCAG

Product: lauroyl/myristoyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MIRYIPSFIFVYLFYLPFRILPYRFCLLYGRFLVFLLYPFAKKHRKIAYENITHAFPEYTETQKKELVWKSILHIGNLVA
GTLFAPRLNQKWMDRYLVYDPESLAIEKKTNEEGIGVVLISGHFGTWEILVQFMGIRMKGGGIYKKVRNPFVDKLIYKLR
TKNGIKLVSTEESSQVTKMLKQGYWVGFGSDQNAGKVGIFVNFFNRPASTYQGPALMAYLTGAKMLLYSVLCGENGKVIV
RVKDLGCVDKKAFPDRETAIRHYTEVWTKALEEEVKLYPEQYFWVHRRWRTKPGDFPGQI

Sequences:

>Translated_300_residues
MIRYIPSFIFVYLFYLPFRILPYRFCLLYGRFLVFLLYPFAKKHRKIAYENITHAFPEYTETQKKELVWKSILHIGNLVA
GTLFAPRLNQKWMDRYLVYDPESLAIEKKTNEEGIGVVLISGHFGTWEILVQFMGIRMKGGGIYKKVRNPFVDKLIYKLR
TKNGIKLVSTEESSQVTKMLKQGYWVGFGSDQNAGKVGIFVNFFNRPASTYQGPALMAYLTGAKMLLYSVLCGENGKVIV
RVKDLGCVDKKAFPDRETAIRHYTEVWTKALEEEVKLYPEQYFWVHRRWRTKPGDFPGQI
>Mature_300_residues
MIRYIPSFIFVYLFYLPFRILPYRFCLLYGRFLVFLLYPFAKKHRKIAYENITHAFPEYTETQKKELVWKSILHIGNLVA
GTLFAPRLNQKWMDRYLVYDPESLAIEKKTNEEGIGVVLISGHFGTWEILVQFMGIRMKGGGIYKKVRNPFVDKLIYKLR
TKNGIKLVSTEESSQVTKMLKQGYWVGFGSDQNAGKVGIFVNFFNRPASTYQGPALMAYLTGAKMLLYSVLCGENGKVIV
RVKDLGCVDKKAFPDRETAIRHYTEVWTKALEEEVKLYPEQYFWVHRRWRTKPGDFPGQI

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=284, Percent_Identity=26.7605633802817, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082094, Length=285, Percent_Identity=24.2105263157895, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35008; Mature: 35008

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYIPSFIFVYLFYLPFRILPYRFCLLYGRFLVFLLYPFAKKHRKIAYENITHAFPEYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETQKKELVWKSILHIGNLVAGTLFAPRLNQKWMDRYLVYDPESLAIEKKTNEEGIGVVLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCHHHEEECCCCCCCCEEEEE
SGHFGTWEILVQFMGIRMKGGGIYKKVRNPFVDKLIYKLRTKNGIKLVSTEESSQVTKML
ECCCCHHHHHHHHHCCEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
KQGYWVGFGSDQNAGKVGIFVNFFNRPASTYQGPALMAYLTGAKMLLYSVLCGENGKVIV
HCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
RVKDLGCVDKKAFPDRETAIRHYTEVWTKALEEEVKLYPEQYFWVHRRWRTKPGDFPGQI
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MIRYIPSFIFVYLFYLPFRILPYRFCLLYGRFLVFLLYPFAKKHRKIAYENITHAFPEYT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ETQKKELVWKSILHIGNLVAGTLFAPRLNQKWMDRYLVYDPESLAIEKKTNEEGIGVVLI
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEEECCHHHEEECCCCCCCCEEEEE
SGHFGTWEILVQFMGIRMKGGGIYKKVRNPFVDKLIYKLRTKNGIKLVSTEESSQVTKML
ECCCCHHHHHHHHHCCEECCCCHHHHHCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
KQGYWVGFGSDQNAGKVGIFVNFFNRPASTYQGPALMAYLTGAKMLLYSVLCGENGKVIV
HCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEE
RVKDLGCVDKKAFPDRETAIRHYTEVWTKALEEEVKLYPEQYFWVHRRWRTKPGDFPGQI
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]