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The map label for this gene is grpE

Identifier: 116329212

GI number: 116329212

Start: 3138240

End: 3138893

Strand: Reverse

Name: grpE

Synonym: LBL_2645

Alternate gene names: 116329212

Gene position: 3138893-3138240 (Counterclockwise)

Preceding gene: 116329213

Following gene: 116329211

Centisome position: 86.84

GC content: 44.65

Gene sequence:

>654_bases
ATGGCCGAAACATCGAATTCGGAAAATAAAACTTCGGAAGAAGCAAAGGCCAGCGAGAAAAATTCTCGGTCAATAACATT
AGAGGAAACTAAATTAGAGAATATGAATTCTGAAGAATCAACACAAACAACTGAATCGACGCAGGCTCAAGCTGCCGAAG
CCGCGGATTCGGAACTTTCGCTGCAAAGCGAATTAGATGCCGCAAAAAAAGAGGTTGAATCCTTGAAGGATTCTTGGGCT
AGGGAAAGAGCCGAATTCCAAAACTTCAAACGTCGTTCCGCACAGGAATTTGTTTCGATCCGTAAGGAAGCGGTCAAGTC
TCTTGTGAGCGGTTTTTTGAATCCGATCGATAACTTGGAAAGGGTCGGCGCGACTCAAAGTCCATCGGAGGAATTAAAAC
CTTTTGTGGAAGGTGTTGCGATGATTCTCAAGGAGTTTTACGCCGTATTAGAAAAATCGAATGTAATTCGTTTCGATCCG
AAGGGGGAATCTTTCGATCCGATGTCGATGGAAGCTCTTTCTTCGGAAGAGGGGGACCAATACTCGGAAGAAACCGTCAT
AGACGTGTATCAAGCCGGCTATTACTACAAGGAAAACGAAGACAAGTTTACGCTTCGGCCTGCAAGGGTTCGAATCGGTA
AACCGAAATCATGA

Upstream 100 bases:

>100_bases
CAGAGAATGGATTACAACAGAGCCTTACCGCTCGTCGACTTTACGTCGAAACTCGTCTCCGAAATGGTGACTCGCATTAG
TAAATAAGGGAGAGTAATGT

Downstream 100 bases:

>100_bases
TTAGAATCTCAGGGAAATATAAAGGAGAAAAACAATGTCCAAAGAAAAGATTATAGGTATCGATTTAGGAACTACTAACT
CGGTCGTTTCCGTTATGGAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MAETSNSENKTSEEAKASEKNSRSITLEETKLENMNSEESTQTTESTQAQAAEAADSELSLQSELDAAKKEVESLKDSWA
RERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQSPSEELKPFVEGVAMILKEFYAVLEKSNVIRFDP
KGESFDPMSMEALSSEEGDQYSEETVIDVYQAGYYYKENEDKFTLRPARVRIGKPKS

Sequences:

>Translated_217_residues
MAETSNSENKTSEEAKASEKNSRSITLEETKLENMNSEESTQTTESTQAQAAEAADSELSLQSELDAAKKEVESLKDSWA
RERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQSPSEELKPFVEGVAMILKEFYAVLEKSNVIRFDP
KGESFDPMSMEALSSEEGDQYSEETVIDVYQAGYYYKENEDKFTLRPARVRIGKPKS
>Mature_216_residues
AETSNSENKTSEEAKASEKNSRSITLEETKLENMNSEESTQTTESTQAQAAEAADSELSLQSELDAAKKEVESLKDSWAR
ERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLERVGATQSPSEELKPFVEGVAMILKEFYAVLEKSNVIRFDPK
GESFDPMSMEALSSEEGDQYSEETVIDVYQAGYYYKENEDKFTLRPARVRIGKPKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_LEPBJ (Q04VC9)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799899.1
- ProteinModelPortal:   Q04VC9
- SMR:   Q04VC9
- STRING:   Q04VC9
- GeneID:   4409332
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0432
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   EVEPNSV
- ProtClustDB:   PRK14146
- BioCyc:   LBOR355277:LBJ_0432-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 24439; Mature: 24308

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETSNSENKTSEEAKASEKNSRSITLEETKLENMNSEESTQTTESTQAQAAEAADSELS
CCCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQSELDAAKKEVESLKDSWARERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGATQSPSEELKPFVEGVAMILKEFYAVLEKSNVIRFDPKGESFDPMSMEALSSEEGDQ
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHCCCCCCC
YSEETVIDVYQAGYYYKENEDKFTLRPARVRIGKPKS
HHHHHHHHHHHCCCEEECCCCCEEECHHHEECCCCCC
>Mature Secondary Structure 
AETSNSENKTSEEAKASEKNSRSITLEETKLENMNSEESTQTTESTQAQAAEAADSELS
CCCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
LQSELDAAKKEVESLKDSWARERAEFQNFKRRSAQEFVSIRKEAVKSLVSGFLNPIDNLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVGATQSPSEELKPFVEGVAMILKEFYAVLEKSNVIRFDPKGESFDPMSMEALSSEEGDQ
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHHHHCCCCCCC
YSEETVIDVYQAGYYYKENEDKFTLRPARVRIGKPKS
HHHHHHHHHHHCCCEEECCCCCEEECHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA