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The map label for this gene is ubiE [H]

Identifier: 116329200

GI number: 116329200

Start: 3117273

End: 3117974

Strand: Reverse

Name: ubiE [H]

Synonym: LBL_2630

Alternate gene names: 116329200

Gene position: 3117974-3117273 (Counterclockwise)

Preceding gene: 116329201

Following gene: 116329199

Centisome position: 86.26

GC content: 39.74

Gene sequence:

>702_bases
ATGATATTATCCGGAAACTTTGAAAATCAGAGAAGGTTCTTCGATCTATTTTCAAAAAGTTACGGTTTTATGGAAATTCT
CACGTTCGGATTCGCAAATCGTGCGAGAAAACGTTTCCTGAAAATTTTGGATCTCACAAACGACGGAATCGTTTGCGATC
TGATGTGCGGAAACGGAAACAATATCGGAATTTTAAGAAAGAATTTTCGTTGTAAAAGGATCATAGGCGTCGACGTTTCG
AGCGGAATGATTCAAGTCGCGCGCGATCGTTTCGGGGAAGAGAATATCTTTTACGTTACCGAAAACGCGCTTGCTTCTTC
AATTCCGTCCAGTCATTGCGATTTTGTGACTTGTAGTTTCGGACTCAAAACGCTAAGACCCGAGCAACGAATTCTTCTGA
TAGACGAGGTGTATCGGATTTTAAAACCGTCCGGTACTTTCGTTTTTATGGAGTTATCCAAACCGGTCGGATTTGTTTCC
GTCTTCTGGAAATTTTATTTTCTGTTTTTTCTGCCTTGGATGGCTAGATTGTTTTCGTATCCGTTTGTACGGAAAAAATA
TTTAATGGATTCGATTTCCGATTTCGGAAGTATTTTTTCCGAGGAAGAATTTTGTCGTAAGGTTTTTTCGAAGTTAAAAT
TCTTTCGTTGGTATGGAGGAATTGTGACCGGAGTTTTGGGAGAAAAAATGGAGACGCCATGA

Upstream 100 bases:

>100_bases
ATCGCCACGTTCTGAACAACGTCAAGAAAAAAGTAGAATCCGAACGAACTCATTCGCATTTTGAAAACGATTAATTCTGA
TTTAAGAATATACTAAAAAT

Downstream 100 bases:

>100_bases
CCGTTTCGATTTTGAGAGATTGTGATTTTGTCAATTCAAGGGAGAGCAATATGAACATCGTCTTTTCTAGAAGCTGTCTC
AAAAATATCTTAGAACGATT

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MILSGNFENQRRFFDLFSKSYGFMEILTFGFANRARKRFLKILDLTNDGIVCDLMCGNGNNIGILRKNFRCKRIIGVDVS
SGMIQVARDRFGEENIFYVTENALASSIPSSHCDFVTCSFGLKTLRPEQRILLIDEVYRILKPSGTFVFMELSKPVGFVS
VFWKFYFLFFLPWMARLFSYPFVRKKYLMDSISDFGSIFSEEEFCRKVFSKLKFFRWYGGIVTGVLGEKMETP

Sequences:

>Translated_233_residues
MILSGNFENQRRFFDLFSKSYGFMEILTFGFANRARKRFLKILDLTNDGIVCDLMCGNGNNIGILRKNFRCKRIIGVDVS
SGMIQVARDRFGEENIFYVTENALASSIPSSHCDFVTCSFGLKTLRPEQRILLIDEVYRILKPSGTFVFMELSKPVGFVS
VFWKFYFLFFLPWMARLFSYPFVRKKYLMDSISDFGSIFSEEEFCRKVFSKLKFFRWYGGIVTGVLGEKMETP
>Mature_233_residues
MILSGNFENQRRFFDLFSKSYGFMEILTFGFANRARKRFLKILDLTNDGIVCDLMCGNGNNIGILRKNFRCKRIIGVDVS
SGMIQVARDRFGEENIFYVTENALASSIPSSHCDFVTCSFGLKTLRPEQRILLIDEVYRILKPSGTFVFMELSKPVGFVS
VFWKFYFLFFLPWMARLFSYPFVRKKYLMDSISDFGSIFSEEEFCRKVFSKLKFFRWYGGIVTGVLGEKMETP

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27109; Mature: 27109

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILSGNFENQRRFFDLFSKSYGFMEILTFGFANRARKRFLKILDLTNDGIVCDLMCGNGN
CEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
NIGILRKNFRCKRIIGVDVSSGMIQVARDRFGEENIFYVTENALASSIPSSHCDFVTCSF
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCCEEEECC
GLKTLRPEQRILLIDEVYRILKPSGTFVFMELSKPVGFVSVFWKFYFLFFLPWMARLFSY
CHHHCCCCHHEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PFVRKKYLMDSISDFGSIFSEEEFCRKVFSKLKFFRWYGGIVTGVLGEKMETP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MILSGNFENQRRFFDLFSKSYGFMEILTFGFANRARKRFLKILDLTNDGIVCDLMCGNGN
CEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
NIGILRKNFRCKRIIGVDVSSGMIQVARDRFGEENIFYVTENALASSIPSSHCDFVTCSF
EEEEEECCCEEEEEEEECCCCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCCCCEEEECC
GLKTLRPEQRILLIDEVYRILKPSGTFVFMELSKPVGFVSVFWKFYFLFFLPWMARLFSY
CHHHCCCCHHEEEHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCC
PFVRKKYLMDSISDFGSIFSEEEFCRKVFSKLKFFRWYGGIVTGVLGEKMETP
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]