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The map label for this gene is accIM [H]

Identifier: 116329163

GI number: 116329163

Start: 3065165

End: 3066802

Strand: Reverse

Name: accIM [H]

Synonym: LBL_2590

Alternate gene names: 116329163

Gene position: 3066802-3065165 (Counterclockwise)

Preceding gene: 116329164

Following gene: 116329162

Centisome position: 84.85

GC content: 39.07

Gene sequence:

>1638_bases
ATGTCTCAAGAGGTAAATCGAAAAAATAAAAATAAATTTCTAGGACAGTTTTTTACACCCGAAAGGGTTGCCGGTTTCCT
GGTAGATTGGATTTTGGGGGCTGAACGTATCACTTCCCAAGAAGGTTTGAAACGTATACTGGACCCTGCAATTGGAAACG
GAATATTTTTCGAAAGCGTTCTTGATAAACTTCCTAACCTCGATGCCGAATGGGTTGGCTTTGATTTGGATGCACAATGT
CTGAGCGCTAGCCGTAGTGCACTTGAAAATAGGATCTCGAAAGCTAGCATACTTAGTTTTTACGATCGCGATTTTTTGTT
ACAGAAGGAGAATCAAAAATTTGATGCCATTCTATGTAATCCTCCGTATCGAAAGATCAGTGATAAAAATTATTCTAGAG
AATTGATTCAGCAATTCGAAGGGAAATCAGAAAGAAAACTTCCTGGAACGGCGAATCTTTATGTTTTCTTTTTATTGAAA
TGTTTAAATCTAATCAATGTAGGAGGTCGGGCGGCTTTTCTCGTTCCTCAAGATTTTTTCAATTCCGGTTACGGAGTTTT
TATTAAGTCTGCATTACAAGAATCAGGTTTGTTGCATTCCTTATTTCTTTTTTCCCCTCAGGACAGTCTTTTTGACGAAG
CGATTACAAGTTCTTGTATTCTTCTATTAGAAAATTCTGAAAAAGAAAAGAAGTCGGGATTTTATTGGACGAGGCTCAAG
CCCGGGTTCTTTTCCGATACATCCAAACTTCCTCTAAGTTCCGTCGAATCGATTCAGACGAATTGGATTTCCTTTCCCGA
TCCGGAAGAAAAATGGAGTCCAATCTTTCATCGACTCGAAAAGAAAACTTATACCGGCGAAAAAAAAGCGGATTTTGAAA
GAAAAAAATTCAACCATTTCGTCCCGTTGACGGAGTTCGGAAAATTTACGAGAGGAATAGCGACCGGAGATAATAATTTC
TTTCTGTTTACGAAAGAGATGGTGGAGGCTTCCGGAATCCCCGAAAAACATTTTAAAACTTGTATTCCAAAATCTCAATA
TGCAAGAAATAGAATATTTTTGCATTCCGATTGGGAGGAGTTGAGTCGAAAAGGCGCTAAGGTCTGGTTGTTGGACGTGA
AGTTGGAATTCGATCCGAAAGATTCCCTCGCGTTGCAGAATCATTTACAATCCGGAATTACGAGAGGAGTCCATAAGAGG
TTTCTACCTTCTAGAAGAAAAAACTGGTACACTCAGGAATCAAAATCTGCATGTCCGATTCTTGCTTCCAGCTTTCATAG
GACCGAAATCAGGATTGTCAGGAATTTTAGCAATGTCGTTCATTTAACTTGTTTTCACGGATTCAAGCCGGCTTTCAATA
TGGAAGAATGGGTGGATGCACTTTATGCATATCTCATTTCTTCGGTTTCTAAAAAGGATCTGGAAGCCAGAAGAAGAGAA
TACGCAAGAGGTCTTTGGAAGGCGGAGCCGGGAGATCTCAATTCTCTTTGGGTTCCTGATTTCAGAAGACTTGATCCTCA
AGTACAAAAAGAATTACAGATTTTGGTCTCCGATTTGAAACAAGCCCGTTTTTCTTCTGAAAAGGAAAATTTCATTCTGC
ATAGAATTGATTCGATTTTTAAGGAAGGATTGATCTGA

Upstream 100 bases:

>100_bases
CTGAAACTTTTGTCTTTGACTGATATCGAGCTGTTGGATATAGAAACATAATTGATTTTGTTTGAAGCTACTAATGAATG
ATTGGAATTGTACGGTATAA

Downstream 100 bases:

>100_bases
TCCGCTTGTGGAAGAAGTCAAAATACACACATCTGGATTGACTCCTGCACGGTTCGAGGGGGTGCCATATCAGAGCGTGT
CCCCAAACCTGAAAAGAAAT

Product: methylase

Products: NA

Alternate protein names: M.AccI; Adenine-specific methyltransferase AccI [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSQEVNRKNKNKFLGQFFTPERVAGFLVDWILGAERITSQEGLKRILDPAIGNGIFFESVLDKLPNLDAEWVGFDLDAQC
LSASRSALENRISKASILSFYDRDFLLQKENQKFDAILCNPPYRKISDKNYSRELIQQFEGKSERKLPGTANLYVFFLLK
CLNLINVGGRAAFLVPQDFFNSGYGVFIKSALQESGLLHSLFLFSPQDSLFDEAITSSCILLLENSEKEKKSGFYWTRLK
PGFFSDTSKLPLSSVESIQTNWISFPDPEEKWSPIFHRLEKKTYTGEKKADFERKKFNHFVPLTEFGKFTRGIATGDNNF
FLFTKEMVEASGIPEKHFKTCIPKSQYARNRIFLHSDWEELSRKGAKVWLLDVKLEFDPKDSLALQNHLQSGITRGVHKR
FLPSRRKNWYTQESKSACPILASSFHRTEIRIVRNFSNVVHLTCFHGFKPAFNMEEWVDALYAYLISSVSKKDLEARRRE
YARGLWKAEPGDLNSLWVPDFRRLDPQVQKELQILVSDLKQARFSSEKENFILHRIDSIFKEGLI

Sequences:

>Translated_545_residues
MSQEVNRKNKNKFLGQFFTPERVAGFLVDWILGAERITSQEGLKRILDPAIGNGIFFESVLDKLPNLDAEWVGFDLDAQC
LSASRSALENRISKASILSFYDRDFLLQKENQKFDAILCNPPYRKISDKNYSRELIQQFEGKSERKLPGTANLYVFFLLK
CLNLINVGGRAAFLVPQDFFNSGYGVFIKSALQESGLLHSLFLFSPQDSLFDEAITSSCILLLENSEKEKKSGFYWTRLK
PGFFSDTSKLPLSSVESIQTNWISFPDPEEKWSPIFHRLEKKTYTGEKKADFERKKFNHFVPLTEFGKFTRGIATGDNNF
FLFTKEMVEASGIPEKHFKTCIPKSQYARNRIFLHSDWEELSRKGAKVWLLDVKLEFDPKDSLALQNHLQSGITRGVHKR
FLPSRRKNWYTQESKSACPILASSFHRTEIRIVRNFSNVVHLTCFHGFKPAFNMEEWVDALYAYLISSVSKKDLEARRRE
YARGLWKAEPGDLNSLWVPDFRRLDPQVQKELQILVSDLKQARFSSEKENFILHRIDSIFKEGLI
>Mature_544_residues
SQEVNRKNKNKFLGQFFTPERVAGFLVDWILGAERITSQEGLKRILDPAIGNGIFFESVLDKLPNLDAEWVGFDLDAQCL
SASRSALENRISKASILSFYDRDFLLQKENQKFDAILCNPPYRKISDKNYSRELIQQFEGKSERKLPGTANLYVFFLLKC
LNLINVGGRAAFLVPQDFFNSGYGVFIKSALQESGLLHSLFLFSPQDSLFDEAITSSCILLLENSEKEKKSGFYWTRLKP
GFFSDTSKLPLSSVESIQTNWISFPDPEEKWSPIFHRLEKKTYTGEKKADFERKKFNHFVPLTEFGKFTRGIATGDNNFF
LFTKEMVEASGIPEKHFKTCIPKSQYARNRIFLHSDWEELSRKGAKVWLLDVKLEFDPKDSLALQNHLQSGITRGVHKRF
LPSRRKNWYTQESKSACPILASSFHRTEIRIVRNFSNVVHLTCFHGFKPAFNMEEWVDALYAYLISSVSKKDLEARRREY
ARGLWKAEPGDLNSLWVPDFRRLDPQVQKELQILVSDLKQARFSSEKENFILHRIDSIFKEGLI

Specific function: This methylase recognizes the double-stranded sequence GTMKAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the AccI endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 63056; Mature: 62924

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQEVNRKNKNKFLGQFFTPERVAGFLVDWILGAERITSQEGLKRILDPAIGNGIFFESV
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDKLPNLDAEWVGFDLDAQCLSASRSALENRISKASILSFYDRDFLLQKENQKFDAILCN
HHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEC
PPYRKISDKNYSRELIQQFEGKSERKLPGTANLYVFFLLKCLNLINVGGRAAFLVPQDFF
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
NSGYGVFIKSALQESGLLHSLFLFSPQDSLFDEAITSSCILLLENSEKEKKSGFYWTRLK
CCCCHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHEEEEEECCCCHHHCCEEEEECC
PGFFSDTSKLPLSSVESIQTNWISFPDPEEKWSPIFHRLEKKTYTGEKKADFERKKFNHF
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
VPLTEFGKFTRGIATGDNNFFLFTKEMVEASGIPEKHFKTCIPKSQYARNRIFLHSDWEE
CCHHHHHHHHCCEECCCCCEEEEEHHHHHHCCCCHHHHHHHCCHHHHHHCEEEEECCHHH
LSRKGAKVWLLDVKLEFDPKDSLALQNHLQSGITRGVHKRFLPSRRKNWYTQESKSACPI
HHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCH
LASSFHRTEIRIVRNFSNVVHLTCFHGFKPAFNMEEWVDALYAYLISSVSKKDLEARRRE
HHHHCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARGLWKAEPGDLNSLWVPDFRRLDPQVQKELQILVSDLKQARFSSEKENFILHRIDSIF
HHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KEGLI
HHCCC
>Mature Secondary Structure 
SQEVNRKNKNKFLGQFFTPERVAGFLVDWILGAERITSQEGLKRILDPAIGNGIFFESV
CCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LDKLPNLDAEWVGFDLDAQCLSASRSALENRISKASILSFYDRDFLLQKENQKFDAILCN
HHHCCCCCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEEC
PPYRKISDKNYSRELIQQFEGKSERKLPGTANLYVFFLLKCLNLINVGGRAAFLVPQDFF
CCCHHCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHH
NSGYGVFIKSALQESGLLHSLFLFSPQDSLFDEAITSSCILLLENSEKEKKSGFYWTRLK
CCCCHHHHHHHHHHCCCCHHHHEECCCHHHHHHHHHHHEEEEEECCCCHHHCCEEEEECC
PGFFSDTSKLPLSSVESIQTNWISFPDPEEKWSPIFHRLEKKTYTGEKKADFERKKFNHF
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
VPLTEFGKFTRGIATGDNNFFLFTKEMVEASGIPEKHFKTCIPKSQYARNRIFLHSDWEE
CCHHHHHHHHCCEECCCCCEEEEEHHHHHHCCCCHHHHHHHCCHHHHHHCEEEEECCHHH
LSRKGAKVWLLDVKLEFDPKDSLALQNHLQSGITRGVHKRFLPSRRKNWYTQESKSACPI
HHCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCH
LASSFHRTEIRIVRNFSNVVHLTCFHGFKPAFNMEEWVDALYAYLISSVSKKDLEARRRE
HHHHCCHHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARGLWKAEPGDLNSLWVPDFRRLDPQVQKELQILVSDLKQARFSSEKENFILHRIDSIF
HHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
KEGLI
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1368703 [H]