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The map label for this gene is birA [H]

Identifier: 116329162

GI number: 116329162

Start: 3063797

End: 3064555

Strand: Reverse

Name: birA [H]

Synonym: LBL_2589

Alternate gene names: 116329162

Gene position: 3064555-3063797 (Counterclockwise)

Preceding gene: 116329163

Following gene: 116329161

Centisome position: 84.79

GC content: 42.82

Gene sequence:

>759_bases
ATGCAGAACGTATTACTTCAACCGGAAAAGGGAATTTTTATGGATTCCGTAACCTCCACAAACTCGGTCATTAAAAGTCG
GGAGTTCCCACACGGAACTTGGATCGTAGCGAAGGAGCAGACCGCTGGAAGAGGAAGAAAGGATCGAACTTGGGAAGTGT
TTGGTGAAGAGTCTTTGATTTTTTCCGGGAAAATCGGTCTAGAAAATTTCGACGGAGGACCGGGACTTTCACTTTTTATA
GGCACCGCAGTTTGTAAGGCGATTCTTTCCGTGTACCCGGAACTCACTCGGGAAATCAAAATCAAATGGCCGAATGATTT
ATATCGTGGTAGCAAAAAGATTGCAGGAATTCTAATAGAGACGGAGGTCGAAGGTTCTTCCGCCACTTTGATCGTAGGAA
TCGGAATGAACTTGTATGGAAAAAGGGAATCGATACCGGCCCATCTTGCGGACGCCGATTATTTGAATACGAATCCGAAT
CGAGCGGGTAAAAAAAACGAGATCATAGAAAAGTTGATACCATTTTTGAATGAAGCGATACTTCTTTGGAAAGATACGGA
AGGAAGAAAAAAGGAACTTATTTTGTTAAACGAACTTCTTCTTTGGAAAGGACAATCCGTCAGTTACACCGAAGATGGAC
AATTGCGAACGGGAGTATTGGAGGGACTTAATGAAAACGGTTCCTTAATCTTAAAAACTCCTTCCGGGATTAAGTTGGAT
TTCATGGATAGTCCCGAGGACTTCCGCTCCCTAAATTAA

Upstream 100 bases:

>100_bases
ATCTCGTCCGTTCATTCAAATTATCATTTCGTAAGAAACGATATTAATTTGGCTTTTTTTCTACTTGCGTAAACCAAATG
ACCTCGCGATGGTAACGGTC

Downstream 100 bases:

>100_bases
AATGCTTCTAGTCGTCGATGTGGGTAATACGAATACCGTCTTTGGGATTTTCGAAAACGGAAAAAACGTTCCTCTGTTTC
ATAAACGAACCGTAACCAGG

Product: biotin--[acetyl-CoA-carboxylase] ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MQNVLLQPEKGIFMDSVTSTNSVIKSREFPHGTWIVAKEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDGGPGLSLFI
GTAVCKAILSVYPELTREIKIKWPNDLYRGSKKIAGILIETEVEGSSATLIVGIGMNLYGKRESIPAHLADADYLNTNPN
RAGKKNEIIEKLIPFLNEAILLWKDTEGRKKELILLNELLLWKGQSVSYTEDGQLRTGVLEGLNENGSLILKTPSGIKLD
FMDSPEDFRSLN

Sequences:

>Translated_252_residues
MQNVLLQPEKGIFMDSVTSTNSVIKSREFPHGTWIVAKEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDGGPGLSLFI
GTAVCKAILSVYPELTREIKIKWPNDLYRGSKKIAGILIETEVEGSSATLIVGIGMNLYGKRESIPAHLADADYLNTNPN
RAGKKNEIIEKLIPFLNEAILLWKDTEGRKKELILLNELLLWKGQSVSYTEDGQLRTGVLEGLNENGSLILKTPSGIKLD
FMDSPEDFRSLN
>Mature_252_residues
MQNVLLQPEKGIFMDSVTSTNSVIKSREFPHGTWIVAKEQTAGRGRKDRTWEVFGEESLIFSGKIGLENFDGGPGLSLFI
GTAVCKAILSVYPELTREIKIKWPNDLYRGSKKIAGILIETEVEGSSATLIVGIGMNLYGKRESIPAHLADADYLNTNPN
RAGKKNEIIEKLIPFLNEAILLWKDTEGRKKELILLNELLLWKGQSVSYTEDGQLRTGVLEGLNENGSLILKTPSGIKLD
FMDSPEDFRSLN

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=133, Percent_Identity=35.3383458646617, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24644246, Length=236, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 27998; Mature: 27998

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNVLLQPEKGIFMDSVTSTNSVIKSREFPHGTWIVAKEQTAGRGRKDRTWEVFGEESLI
CCCCEECCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCEEE
FSGKIGLENFDGGPGLSLFIGTAVCKAILSVYPELTREIKIKWPNDLYRGSKKIAGILIE
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCCEEEEEEEE
TEVEGSSATLIVGIGMNLYGKRESIPAHLADADYLNTNPNRAGKKNEIIEKLIPFLNEAI
EECCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
LLWKDTEGRKKELILLNELLLWKGQSVSYTEDGQLRTGVLEGLNENGSLILKTPSGIKLD
EEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCEEE
FMDSPEDFRSLN
ECCCCHHHHCCC
>Mature Secondary Structure
MQNVLLQPEKGIFMDSVTSTNSVIKSREFPHGTWIVAKEQTAGRGRKDRTWEVFGEESLI
CCCCEECCCCCCEEECCCCHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCEEEECCCEEE
FSGKIGLENFDGGPGLSLFIGTAVCKAILSVYPELTREIKIKWPNDLYRGSKKIAGILIE
EECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHCCCEEEEEEEE
TEVEGSSATLIVGIGMNLYGKRESIPAHLADADYLNTNPNRAGKKNEIIEKLIPFLNEAI
EECCCCCEEEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCEE
LLWKDTEGRKKELILLNELLLWKGQSVSYTEDGQLRTGVLEGLNENGSLILKTPSGIKLD
EEEECCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCCEEEECCCCCEEE
FMDSPEDFRSLN
ECCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]